Immunological detection of a GarV-Type virus in Argentine garlic cultivars
A cDNA library representing the genomes of several garlic viruses was generated using a viral RNA mixture as template. Analysis of randomly selected clones allowed the identification of different vital genomic sequences. On the basis of amino acid and nucleotide sequence comparisons, one of them was...
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American Phytopathological Society
1997
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| LEADER | 10182caa a22010697a 4500 | ||
|---|---|---|---|
| 001 | PAPER-3187 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203239.0 | ||
| 008 | 190411s1997 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0030880097 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a PLDID | ||
| 100 | 1 | |a Helguera, M. | |
| 245 | 1 | 0 | |a Immunological detection of a GarV-Type virus in Argentine garlic cultivars |
| 260 | |b American Phytopathological Society |c 1997 | ||
| 270 | 1 | 0 | |m Mentaberry, A.; IIIG, Biologia Molecular (INGEBI-CONICET), Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina; email: armenta@proteus.dna.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Ausubel, F.M., Brent, J.A., Kingston, R., Moore, D., Seidman, J.D., Smith, J.A., Struthl, K., (1994) Current Protocols in Molecular Biology, , Karen Joanne ed. John Wiley & Sons, New York | ||
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| 504 | |a Conci, V.C., Nome, S.F., Virus free garlic (Allium sativum L.) plants obtained by thermotherapy and meristem tip culture (1991) J. Phytopathol., 132, pp. 186-192 | ||
| 504 | |a Conci, V.C., Nome, S.F., Milne, R.G., Filamentous viruses of garlic in Argentina (1992) Plant Dis., 76, pp. 594-596 | ||
| 504 | |a Dellecole, B., Lot, H., Viroses de Tail: I Mise en évidence et essais de caractérisation par immunoélectromicroscopie d'un complexe de trois virus chez différentes population d'ail atteintes de mosaïque (1981) Agronomie, 1, pp. 763-769 | ||
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| 504 | |a Hull, R., Purification, biophysical and biochemical characterization of viruses with especial reference to plant viruses (1985) Virology: A Practical Approach, pp. 1-14. , B. W. J. Mahy, ed. IRL Press, Oxford | ||
| 504 | |a Kanyuka, K.V., Vishnichenko, V.K., Leva, K.E., Kondrikov, D.Y., Ryabov, E.V., Zavriev, S.K., Nucleotide sequence of shallot virus X RNA reveals a 5′-proximal cistron closely related to those of potexviruses and a unique arrangement of the 3′- Proximal cistrons (1992) J. Gen. Virol., 73, pp. 2553-2560 | ||
| 504 | |a Kobayashi, K., Rabinowicz, P., Bravo-Almon-Acid, F., Helguera, M., Conci, V., Lot, H., Mentaberry, A.N., Coat protein gene sequences of garlic and onion isolates of the onion yellow dwarf potyvirus (OYDV) Arch. Virol., 141, pp. 2277-2287 | ||
| 504 | |a Koch, M., Salomon, R., Serological detection of onion yellow dwarf virus in garlic (1994) Plant Dis., 78, pp. 785-788 | ||
| 504 | |a Maniatis, T., Fritsch, E.F., Sambrook, J., (1988) Molecular Cloning: A Laboratory Manual, , Cold Spring Harbor Laboratory Press, New York | ||
| 504 | |a Milne, R.G., Luisoni, E., Rapid immune electron microscopy of virus preparations (1977) Methods Virol., 6, pp. 265-281 | ||
| 504 | |a Murphy, F.A., Fauquet, C.M., Bishop, D.H.L., Ghabrial, S.A., Martelli, G.P., Mayo, A., Summers, M.D., Virus taxonomy (1995) 6th Rep. Int. Commit. Taxonomy Viruses, , Springer-Verlag, Vienna | ||
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| 504 | |a Nagakubo, T., Kubo, M., Oeda, K., Nucleotide sequences of 3′ regions of two major viruses from mosaic-diseases in garlic: Molecular evidence of mixed infection by a potyvirus and a carlavirus (1994) Phytopathology, 84, pp. 640-645 | ||
| 504 | |a Sanger, F., Nicklen, S., Coulson, A., DNA sequencing with chain-terminating inhibitors (1977) Proc. Natl. Acad. Sci. USA, 74, pp. 5463-5467 | ||
| 504 | |a Sumi, S., Tsuneyoshi, T., Furutani, H., Novel rod-shaped viruses isolated from garlic, Allium sativum, possessing a unique genome organization (1993) J. Gen. Virol., 72, pp. 9-14 | ||
| 504 | |a Tsuneyoshi, T., Sumi, S., Differentiation among garlic viruses in mixed infections based on RT-PCR procedures and direct tissue blotting immunoassays (1996) Phytopathology, 86, pp. 253-259 | ||
| 504 | |a Van Dijk, P., Carlavirus isolates from cultivated Allium sp. represent three viruses (1993) Neth. J. Plant. Pathol., 99, pp. 233-257 | ||
| 504 | |a Van Dijk, P., Survey and characterization of Potyviruses and their strains of Allium sp (1993) Neth. J. Plant. Pathol., 99 (SUPPL. 2), pp. 1-48 | ||
| 504 | |a Van Dijk, P., Van der Vlugt, R.A.A., New mite-borne virus isolates from rakkyo, shallot and wild leek species (1994) Eur. J. Plant. Pathol., 100, pp. 269-277 | ||
| 504 | |a Van Dijk, P., Verbeck, M., Bos, L., Mite-borne virus isolates from cultivated Allium sp. and their classification into two new rymoviruses in the family Potyviridae (1991) Neth. J. Plant. Pathol., 97, pp. 381-399 | ||
| 504 | |a Walkey, D.G.A., Virus Diseases (1990) Onions and Allied Crops, 2, pp. 191-212. , Rabinowitch, H. D., and Brewster, J. L., eds. CRC Press, Boca Ratón, FL | ||
| 504 | |a Walkey, D.G.A., Antill, D.N., Agronomic evaluation of virus-free and virus infected garlic (1989) J. Hortic. Sci., 64, pp. 53-64 | ||
| 504 | |a Walkey, D.G.A., Webb, M.J.W., Bolland, C.J., Miller, A., Production of virus- Free garlic (Allium sativum L.) and shallot (A. ascalonicum L.) by meristem-tip culture (1987) J. Hortic. Sci., 62, pp. 211-220 | ||
| 504 | |a Yamashita, K., Purification and serology of garlic mite-borne mosaic virus (GMbMV) (1993) Ann. Phytopathol. Soc. Jpn., 59, p. 57 | ||
| 504 | |a Yamashita, K., Sakai, J., Hanada, K., Leek yellow stripe virus (LYSV) isolated from garlic and its relationship to garlic mosaic virus (GMV) (1995) Ann. Phytopathol. Soc. Jpn., 61, p. 273 | ||
| 520 | 3 | |a A cDNA library representing the genomes of several garlic viruses was generated using a viral RNA mixture as template. Analysis of randomly selected clones allowed the identification of different vital genomic sequences. On the basis of amino acid and nucleotide sequence comparisons, one of them was assigned to garlic virus A (GarV-A), a novel flexuous, rod-shaped virus recently reported in Japan. The coat protein (CP) of this virus was expressed in Escherichia colt cells and used as immunogen to produce polyclonal antibodies. The expression protein was recognized by an antiserum detecting garlic mite-borne filamentous virus, but did not react with antibodies specific for other garlic viruses. Antibodies raised against the vital CP reacted with extracts infected with garlic mite-borne viruses and fail to recognize preparations of onion yellow dwarf petyvirus, leek yellow stripe potyvirus, and carnation latent carlavirus. The same antibodies decorated vital particles exhibiting a modal length of 586 nm in immuno electron microscopy with decoration assays. Double-antibody enzyme-linked immunosorbent assays and tissue printing assays performed with these antibodies allowed detection of GarV-A in most garlic cultivars used in Argentina. |l eng | |
| 593 | |a Inst. de Invest. en Ing. Genet. y B., Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina | ||
| 593 | |a Inst. de Fitopatologia y Fisiol. V., Camino 60 Cuadras Km 5 1/2, CP 5014, Córdoba, Argentina | ||
| 593 | |a INGEBI-CONICET, FCEyN-UBA, Argentina | ||
| 593 | |a IFFIVE-INTA, Argentina | ||
| 690 | 1 | 0 | |a ACARI |
| 690 | 1 | 0 | |a ALLIUM CEPA |
| 690 | 1 | 0 | |a ALLIUM PORRUM |
| 690 | 1 | 0 | |a ALLIUM SATIVUM |
| 690 | 1 | 0 | |a CARLAVIRUS |
| 690 | 1 | 0 | |a DIANTHUS CARYOPHYLLUS |
| 690 | 1 | 0 | |a ESCHERICHIA |
| 690 | 1 | 0 | |a GARLIC MITE-BORNE FILAMENTOUS VIRUS |
| 690 | 1 | 0 | |a GARLIC VIRUS A |
| 690 | 1 | 0 | |a LEEK YELLOW STRIPE POTYVIRUS |
| 690 | 1 | 0 | |a POTYVIRUS |
| 690 | 1 | 0 | |a ACARI |
| 690 | 1 | 0 | |a ALLEXIVIRUS |
| 690 | 1 | 0 | |a ALLIUM CEPA |
| 690 | 1 | 0 | |a ALLIUM PORRUM |
| 690 | 1 | 0 | |a ALLIUM SATIVUM |
| 690 | 1 | 0 | |a CARLAVIRUS |
| 690 | 1 | 0 | |a DIANTHUS CARYOPHYLLUS |
| 690 | 1 | 0 | |a ESCHERICHIA COLI |
| 690 | 1 | 0 | |a GARLIC MITE-BORNE FILAMENTOUS VIRUS |
| 690 | 1 | 0 | |a GARLIC VIRUS A |
| 690 | 1 | 0 | |a LEEK YELLOW STRIPE VIRUS |
| 690 | 1 | 0 | |a ONION YELLOW DWARF VIRUS |
| 690 | 1 | 0 | |a POTYVIRUS |
| 651 | 4 | |a ARGENTINA (FISH) | |
| 700 | 1 | |a Bravo-Almonacid, F. | |
| 700 | 1 | |a Kobayashi, K. | |
| 700 | 1 | |a Rabinowicz, P.D. | |
| 700 | 1 | |a Conci, V. | |
| 700 | 1 | |a Mentaberry, A. | |
| 773 | 0 | |d American Phytopathological Society, 1997 |g v. 81 |h pp. 1005-1010 |k n. 9 |p PLANT DIS. |x 01912917 |t Plant Disease | |
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