DNA supercoiling and osmoresistance in Bacillus subtilis 168
The importance of the DNA structure for the expression of the osmotic response (osmotolerance) was investigated in Bacillus subtilis 168. Plasmid pUB110 DNA was used as a reporter of the chromosomal DNA topology, and analyses were performed in chloroquine agarose gels. Plasmidic DNA obtained from cu...
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| Formato: | Capítulo de libro |
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1997
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 11677caa a22011177a 4500 | ||
|---|---|---|---|
| 001 | PAPER-3152 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203237.0 | ||
| 008 | 190411s1997 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0030657007 | |
| 024 | 7 | |2 cas |a Anti-Bacterial Agents; Chloroquine, 54-05-7; Culture Media; DNA Gyrase, EC 5.99.1.-; DNA Topoisomerases, Type II, EC 5.99.1.3; DNA, Bacterial; DNA, Superhelical; Novobiocin, 303-81-1 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a CUMID | ||
| 100 | 1 | |a Alice, A.F. | |
| 245 | 1 | 0 | |a DNA supercoiling and osmoresistance in Bacillus subtilis 168 |
| 260 | |c 1997 | ||
| 270 | 1 | 0 | |m Sanchez-Rivas, C.; Departmento de Quimica Biologica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon II, Piso 4. (1428) Buenos Aires, Argentina; email: sanchez@qb.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Birnboim, H., Doly, J., A rapid alkaline extraction procedure for screening recombinant plasmid DNA (1979) Nucleic Acid Res, 7, pp. 897-903 | ||
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| 504 | |a Gellert, M., O'Dea, M.H., Itoh, T., Tomizawa, J.I., Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase (1976) Proc Natl Acad Sci USA, 73, pp. 4474-4478 | ||
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| 504 | |a Kunst, F., Rapoport, G., Salt stress is an environmental signal affecting degradative enzyme synthesis in Bacillus subtilis (1995) J Bacteriol, 177, pp. 2403-2407 | ||
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| 504 | |a Owen-Hughes, T.A., Pavitt, G.D., Santos, D.S., Sidebotham, J.M., Hulton, C.S.J., Hinton, J.C.D., Higgins, C.F., The chromatin-associated protein H-NS interacts with curved DNA to influence DNA topology and gene expression (1992) Cell, 71, pp. 255-265 | ||
| 504 | |a Pruss, G.J., Drlica, K., DNA supercoiling and prokaryotic transcription (1989) Cell, 56, pp. 521-523 | ||
| 504 | |a Rubinstein, C.P., Moratinos, L.C., Coso, O.A., Sanchez-Rivas, C., Improvements in the transformation of Bacillus subtilis protoplasts with plasmid DNA (1988) FEMS Microbiol Lett, 56, pp. 67-70 | ||
| 504 | |a Ruzal, S.M., Sanchez-Rivas, C., Physiological and genetic characterization of the osmotic stress response in Bacillus subtilis (1994) Can J Microbiol, 40, pp. 140-144 | ||
| 504 | |a Ruzal, S.M., Alice, A.F., Sanchez-Rivas, C., Osmotolerance of spores from Bacillus subtilis and the effect of ssp mutations (1994) Microbiology, 140, pp. 2173-2177 | ||
| 504 | |a Salti, V., Le Hégarat, F., Hirschbein, L., Isolation and characterization of small heat-stable acid soluble DNA-binding proteins from Bacillus subtilis (1985) J Gen Microbiol, 131, pp. 581-590 | ||
| 504 | |a Schaeffer, P., Millet, J., Aubert, J., Catabolic repression of bacterial sporulation (1965) Proc Natl Acad Sci USA, 54, pp. 701-711 | ||
| 504 | |a Sheehan, B.J., Foster, T.J., Dorman, C.J., Park, S., Stewart, G.S.A.B., Osmotic and growth-phase dependent regulation of the eta gene of Staphylococcus aureus: A role for DNA supercoiling (1992) Mol Gen Genet, 232, pp. 49-57 | ||
| 504 | |a Tanaka, H., Yasuzawa, K., Kohno, K., Goshima, N., Kano, Y., Saiki, T., Imamoto, F., Role of HU proteins in forming and constraining supercoils of chromosomal DNA in Escherichia coli (1995) Mol Gen Genet, 248, pp. 518-526 | ||
| 504 | |a Wang, J.Y., Syvanen, M., DNA twist as a transcriptional sensor for environmental changes (1992) Mol Microbiol, 6, pp. 1861-1866 | ||
| 504 | |a Whatmore, A.M., Chudek, J.A., Reed, R.H., The effects of osmotic upshock on the intracellular solute pools of Bacillus subtilis (1990) J Gen Microbiol, 136, pp. 2527-2535 | ||
| 520 | 3 | |a The importance of the DNA structure for the expression of the osmotic response (osmotolerance) was investigated in Bacillus subtilis 168. Plasmid pUB110 DNA was used as a reporter of the chromosomal DNA topology, and analyses were performed in chloroquine agarose gels. Plasmidic DNA obtained from cultures in Schaeffer medium (D) taken in those periods in which B. subtilis is able to express osmotolerance (early stationary phase or from germinating spores) or from adapted cultures to hyperosmotic medium (DN) presented a higher level of negative supercoiling than DNA samples from vegetative cultures, normally refractory to induction of osmotolerance. The involvement of the DNA gyrase was investigated through the sensitivity to novobiocin, an antibiotic inhibitor of its activity and the behavior of a gyrB1 mutant strain (RG1). In the wild-type strain, the addition of a sublethal concentration of novobiocin (0.5 μg/ml) to the hyperosmotic medium relaxed DNA and inhibited growth. Moreover, already growing cultures in DN medium and later submitted to the same antibiotic presented a relaxed DNA and stopped growing. The RG1 mutant strain submitted to similar novobiocin treatments displayed normal growth in DN novobiocin medium. These results pointed to the requirement of a highly negative supercoiled DNA structure involving the gyrase activity in osmotic response. |l eng | |
| 593 | |a Departmento de Quim. Biol., Universidad de Buenos Aires, Pabellón II, (1428) Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a DNA TOPOISOMERASE (ATP HYDROLYSING) |
| 690 | 1 | 0 | |a NOVOBIOCIN |
| 690 | 1 | 0 | |a PLASMID DNA |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a BACILLUS SUBTILIS |
| 690 | 1 | 0 | |a BACTERIAL GROWTH |
| 690 | 1 | 0 | |a BACTERIUM MUTANT |
| 690 | 1 | 0 | |a DNA SUPERCOILING |
| 690 | 1 | 0 | |a ENZYME ACTIVITY |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a OSMOREGULATION |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a ANTI-BACTERIAL AGENTS |
| 690 | 1 | 0 | |a BACILLUS SUBTILIS |
| 690 | 1 | 0 | |a CHLOROQUINE |
| 690 | 1 | 0 | |a CULTURE MEDIA |
| 690 | 1 | 0 | |a DNA GYRASE |
| 690 | 1 | 0 | |a DNA TOPOISOMERASES, TYPE II |
| 690 | 1 | 0 | |a DNA, BACTERIAL |
| 690 | 1 | 0 | |a DNA, SUPERHELICAL |
| 690 | 1 | 0 | |a GENES, REPORTER |
| 690 | 1 | 0 | |a NOVOBIOCIN |
| 690 | 1 | 0 | |a OSMOTIC PRESSURE |
| 690 | 1 | 0 | |a PLASMIDS |
| 690 | 1 | 0 | |a SPORES, BACTERIAL |
| 690 | 1 | 0 | |a BACILLUS SUBTILIS |
| 690 | 1 | 0 | |a BACTERIA (MICROORGANISMS) |
| 690 | 1 | 0 | |a BACTERIUM SUBTILIS |
| 700 | 1 | |a Sanchez-Rivas, C. | |
| 773 | 0 | |d 1997 |g v. 35 |h pp. 309-315 |k n. 5 |p CURR. MICROBIOL. |x 03438651 |w (AR-BaUEN)CENRE-160 |t Current Microbiology | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030657007&doi=10.1007%2fs002849900260&partnerID=40&md5=7cae31a657542a2f239e1aaca0acb76d |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s002849900260 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_03438651_v35_n5_p309_Alice |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v35_n5_p309_Alice |y Registro en la Biblioteca Digital |
| 961 | |a paper_03438651_v35_n5_p309_Alice |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 64105 | ||