Differential regulation of putrescine uptake in trypanosoma cruzi and other trypanosomatids

Putrescine uptake in Trypanosoma cruzi epimastigotes is 10 to 50-fold higher than in Leishmania mexicana or Crithidia fasciculata. Polyamine transport in all these trypanosomatids is an energy-dependent process strongly inhibited by the presence of 2,4-dinitrophenol or KCN. Putrescine uptake in T. c...

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Autor principal: Gonzalez, N.S
Otros Autores: Ceriani, C., Algranati, I.D
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1992
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Acceso en línea:Registro en Scopus
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024 7 |2 scopus  |a 2-s2.0-0026451048 
024 7 |2 cas  |a Eflornithine, 70052-12-9; Putrescine, 110-60-1 
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100 1 |a Gonzalez, N.S. 
245 1 0 |a Differential regulation of putrescine uptake in trypanosoma cruzi and other trypanosomatids 
260 |c 1992 
270 1 0 |m Gonzalez, N.S.; Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, A. Machado 151, 1405 Buenos Aires, Argentina 
506 |2 openaire  |e Política editorial 
504 |a Pegg, (1986) Biochem. J, 234, pp. 249-262 
504 |a Russell, (1989) The Physiology of Polyamines, 1, pp. 255-279. , U. Bachrach, Y.M. Heimer, 2nd ed, C.R.C. Press Inc, Boca Ratón, FL 
504 |a Jänne, Pösö, Raina, (1978) Biochim. Biophys. Acta, 473, pp. 241-293 
504 |a Pegg, (1988) Cancer Res, 48, pp. 759-774 
504 |a Olson, Russell, (1980) Cancer Res, 40, pp. 4373-4380 
504 |a Brandt, Pierce, Fausto, (1972) Biochim. Biophys. Acta, 279, pp. 184-193 
504 |a Sogani, Matsushita, Mueller, Raben, (1972) Biochim. Biophys. Acta, 279, pp. 377-386 
504 |a Oka, Borellini, (1989) Ornithine Decarboxylase: Biology, Enzymology and Molecular Genetics, pp. 7-19. , S. Hayashi, Pergamon Press, New York 
504 |a Pohjanpelto, (1976) J. Cell Biol, 68, pp. 512-520 
504 |a Kano, Oka, (1976) J. Biol. Chem, 251, pp. 2795-2800 
504 |a Bethell, Pegg, (1981) J. Cell Physiol, 109, pp. 461-468 
504 |a McCann, Pegg, Sjoerdsma, (1987) Inhibition of Polyamine Metabolism. Biological Significance and Basis for New Therapies, , Academic Press, New York 
504 |a Pera, Kramer, Sufrin, Porter, (1986) Cancer Res, 46, pp. 1148-1154 
504 |a Gonzalez, Sánchez, Sferco, Algranati, (1991) Biochem. Biophys. Res. Commun, 180, pp. 797-804 
504 |a Seiler, Dezeure, Polyamine transport in mammalian cells (1990) International Journal of Biochemistry, 20, pp. 211-218 
504 |a Khan, Quemener, Moulinoux, (1991) Cell Biol. Int. Rep, 15, pp. 9-24 
504 |a Ceriani, Gonzalez, Algranati, (1992) FEBS Lett, 301, pp. 261-264 
504 |a Rinehart, Jr., Chen, (1984) J. Biol. Chem, 259, pp. 4750-4756 
504 |a Alhonen-Hongisto, Sëppanen, Jänne, (1980) Biochem. J, 192, pp. 941-945 
504 |a Kakinuma, Hoshino, Igarashi, (1988) Eur. J. Biochem, 176, pp. 409-414 
504 |a Mc Cann, Bacchi, Clarkson, Jr., Seed, Nathan, Amole, Hutner, Sjoerdsma, (1981) Med. Biol, 59, pp. 434-440 
504 |a Balaña Fouce, Escribano, Alunda, (1991) Mol. Cell. Biochem, 107, pp. 127-133 
504 |a Algranati, Sánchez, Gonzalez, (1990) The Biology and Chemistry of Polyamines, pp. 137-146. , S.H. Goldemberg, I.D. Algranati, ICSU-IRL Press, Oxford 
520 3 |a Putrescine uptake in Trypanosoma cruzi epimastigotes is 10 to 50-fold higher than in Leishmania mexicana or Crithidia fasciculata. Polyamine transport in all these trypanosomatids is an energy-dependent process strongly inhibited by the presence of 2,4-dinitrophenol or KCN. Putrescine uptake in T. cruzi and L. mexicana was markedly decreased by the proton ionophore carbonylcyanide m-chlorophenylhydrazone but it was not affected by ouabain, a Na+-K+ pump inhibitor. The depletion of intracellular polyamines by treatment of parasite cultures with α-difluoromethylornithine elicited a marked induction of putrescine uptake in L. mexicana and C. fasciculata by increasing considerably the Vmax of this process. Conversely, the uptake of putrescine in T. cruzi was essentially unchanged by the same treatment. The differential regulation of putrescine transport in T. cruzi might be related to some distinctive features of polyamine metabolism in this parasite. © 1992.  |l eng 
593 |a Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, A. Machado 151, 1405 Buenos Aires, Argentina 
690 1 0 |a 2,4 DINITROPHENOL 
690 1 0 |a CARBONYL CYANIDE CHLOROPHENYLHYDRAZONE 
690 1 0 |a POTASSIUM CYANIDE 
690 1 0 |a PUTRESCINE 
690 1 0 |a AMINE TRANSPORT 
690 1 0 |a ARTICLE 
690 1 0 |a EPIMASTIGOTE 
690 1 0 |a LEISHMANIA MEXICANA 
690 1 0 |a NONHUMAN 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a TRYPANOSOMA CRUZI 
690 1 0 |a ANIMAL 
690 1 0 |a BIOLOGICAL TRANSPORT 
690 1 0 |a CRITHIDIA FASCICULATA 
690 1 0 |a EFLORNITHINE 
690 1 0 |a KINETICS 
690 1 0 |a LEISHMANIA MEXICANA 
690 1 0 |a PUTRESCINE 
690 1 0 |a SUPPORT, NON-U.S. GOV'T 
690 1 0 |a TEMPERATURE 
690 1 0 |a TRYPANOSOMA CRUZI 
690 1 0 |a CRITHIDIA FASCICULATA 
690 1 0 |a LEISHMANIA MEXICANA 
690 1 0 |a STROPHANTHUS GRATUS 
690 1 0 |a TRYPANOSOMA 
690 1 0 |a TRYPANOSOMA CRUZI 
690 1 0 |a TRYPANOSOMATIDAE 
650 1 7 |2 spines  |a HOMEOSTASIS 
700 1 |a Ceriani, C. 
700 1 |a Algranati, I.D. 
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