Early inhibition of DNA synthesis in the developing rat cerebral cortex by the purine analogues olomoucine and roscovitine

The effects of the cyclin-dependent kinase (CDK) inhibitors olomoucine and roscovitine on DNA synthesis were studied using short time incubation (30-90 minutes). Both purine analogues at concentrations from 1-100 μM decreased the DNA synthesis of rat brain cortex in a dose-dependent manner and the m...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autor principal: Yakisich, J.S
Otros Autores: Sidén, A., Vargas, V.I, Eneroth, P., Cruz, M.
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Academic Press Inc. 1998
Acceso en línea:Registro en Scopus
DOI
Handle
Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
LEADER 05779caa a22008177a 4500
001 PAPER-2986
003 AR-BaUEN
005 20230518203227.0
008 190411s1998 xx ||||fo|||| 00| 0 eng|d
024 7 |2 scopus  |a 2-s2.0-0032562099 
024 7 |2 cas  |a cyclin dependent kinase, 150428-23-2 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a BBRCA 
100 1 |a Yakisich, J.S. 
245 1 0 |a Early inhibition of DNA synthesis in the developing rat cerebral cortex by the purine analogues olomoucine and roscovitine 
260 |b Academic Press Inc.  |c 1998 
270 1 0 |m Yakisich, J.S.; Applied Biochemistry, Clinical Research Center, Huddinge University Hospital, S-141 86 Huddinge, Sweden 
506 |2 openaire  |e Política editorial 
504 |a Vesely, J., Havlicek, L., Strnad, M., Blow, J.J., Donella-Deana, A., Pinna, L., Letham, D.S., Meijer, L., (1994) Eur. J. Biochem., 224, pp. 771-786 
504 |a Meijer, L., Borgne, A., Mulner, O., Chong, J.P.J., Blow, J.J., Inagaki, N., Inagaki, M., Moulinoux, J-P., (1997) Eur. J. Biochem., 243, pp. 527-536 
504 |a Glab, N., Labidi, B., Qin, L-X., Trehin, C., Bergounioux, C., Meijer, L., (1994) FEBS Lett., 353, pp. 207-211 
504 |a Abraham, R.T., Acquarone, M., Andersen, A., Asensi, A., Belle, R., Berger, F., Bergounioux, C., Verlhac, M.H., (1995) Biol. Cell., 83, pp. 105-120 
504 |a Meijer, L., (1996) Trends Cell Biol, 6, pp. 393-397 
504 |a Alperin, D.M., Idoyaga-Vargas, V.P., Carminatti, H., (1986) J. Neurochem., 47, pp. 355-362 
504 |a Labarca, C., Paigen, K., (1980) Anal. Biochem., 102, pp. 344-352 
504 |a Nurse, P., Bisset, Y., (1981) Nature, 292, pp. 558-560 
504 |a Gadbois, D.M., Hamaguchi, J.R., Swank, R.A., Bradbury, M.E., (1992) Biochem. Biophys. Res. Comm., 184, pp. 80-85 
504 |a Veerana, S.K.T., Amin, N., Grant, P., Albers, R.W., Pant, H.C., (1996) Neurochem. Res., 21, pp. 629-636 
504 |a Hall, L.L., Th'Ng, J.P.H., Guo, X.W., Teplitz, R.L., Bradbury, M.E., (1996) Cancer Res., 56, pp. 3551-3559 
504 |a Yakisich, J.S., Radrizzani, M., Idoyaga Vargas, V., (1994) Neurosci. Lett., 180, pp. 17-20 
504 |a Blow, J.J., Laskey, R.A., (1988) Nature, 332, pp. 546-548 
504 |a Chong, J.P.J., Mahbubani, H.M., Khoo, C-Y., Blow, J.J., (1995) Nature, 375, pp. 418-421 
504 |a Shields, R., (1977) Nature, 267, pp. 704-707 
504 |a McAuley, A., Werb, Z., Mirkes, P.E., (1993) Development, 117, pp. 873-883 
504 |a Weinert, T.A., Hartwell, L.H., (1988) Science, 241, pp. 317-322 
504 |a Hartwell, L.H., Weinert, T.A., (1989) Science, 246, pp. 629-634 
504 |a Hartwell, L.H., Kastan, M.B., (1994) Science, 266, pp. 1821-1828 
520 3 |a The effects of the cyclin-dependent kinase (CDK) inhibitors olomoucine and roscovitine on DNA synthesis were studied using short time incubation (30-90 minutes). Both purine analogues at concentrations from 1-100 μM decreased the DNA synthesis of rat brain cortex in a dose-dependent manner and the maximum effect occurred within 30 min of incubation. Staurosporine, another potent CDK inhibitor did not affect the DNA synthesis in the concentration range 1-250 nM. These results indicate that olomoucine and roscovitine block DNA synthesis by a mechanism independent of CDK inhibition. We propose that the cellullar effects of olomuocine and roscovitine on the cell cycle are at least in part due to this early inhibitory effect on DNA synthesis.  |l eng 
536 |a Detalles de la financiación: Universidad de Buenos Aires 
536 |a Detalles de la financiación: Karolinska Institutet, KI 
536 |a Detalles de la financiación: This study was supported by grants from the Karolinska Institute, the Swedish Medical Association and Consejo Nacional de Investi-gaciones CientõB ficas y TeÂcnicas (CONICET) as well as the University of Buenos Aires. The access to resources at the Clinical Research Center, Novum, is gratefully acknowledged. 
593 |a Applied Biochemistry, Clinical Research Center, Huddinge University Hospital, S-141 86, Huddinge, Sweden 
593 |a Department of Neurology, Karolinska Institute, Huddinge University Hospital, S-141 86, Huddinge, Sweden 
593 |a Inst. Invest. Bioquimicas Fund. C., Fac. de Ciencias Exactas y Naturales, Buenos Aires, Argentina 
690 1 0 |a CYCLIN DEPENDENT KINASE 
690 1 0 |a CYCLIN DEPENDENT KINASE INHIBITOR 
690 1 0 |a PURINE DERIVATIVE 
690 1 0 |a ANIMAL CELL 
690 1 0 |a ANIMAL TISSUE 
690 1 0 |a ARTICLE 
690 1 0 |a BRAIN CORTEX 
690 1 0 |a CELL CYCLE 
690 1 0 |a DNA SYNTHESIS INHIBITION 
690 1 0 |a NONHUMAN 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a RAT 
690 1 0 |a TIME 
690 1 0 |a ANIMALIA 
700 1 |a Sidén, A. 
700 1 |a Vargas, V.I. 
700 1 |a Eneroth, P. 
700 1 |a Cruz, M. 
773 0 |d Academic Press Inc., 1998  |g v. 243  |h pp. 674-677  |k n. 3  |p Biochem. Biophys. Res. Commun.  |x 0006291X  |w (AR-BaUEN)CENRE-905  |t Biochemical and Biophysical Research Communications 
856 4 1 |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032562099&doi=10.1006%2fbbrc.1998.8157&partnerID=40&md5=1cf9873abe912d94c2ad72255ff7dbbd  |y Registro en Scopus 
856 4 0 |u https://doi.org/10.1006/bbrc.1998.8157  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_0006291X_v243_n3_p674_Yakisich  |y Handle 
856 4 0 |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0006291X_v243_n3_p674_Yakisich  |y Registro en la Biblioteca Digital 
961 |a paper_0006291X_v243_n3_p674_Yakisich  |b paper  |c PE 
962 |a info:eu-repo/semantics/article  |a info:ar-repo/semantics/artículo  |b info:eu-repo/semantics/publishedVersion 
963 |a VARI 
999 |c 63939