Isolation, partial characterization and biological activity of mannosyl glycopeptides from seminal plasma
Affinity chromatography on Concanavalin-A Sepharose, followed by gel filtration and hydrophobic interaction chromatography, permits the isolation of low molecular weight N-glycosidically linked oligomannosidic glycopeptides (MGp) from the autoproteolysis products of human seminal plasma. The monosac...
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| Otros Autores: | , , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
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1998
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 06892caa a22010937a 4500 | ||
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| 001 | PAPER-2909 | ||
| 003 | AR-BaUEN | ||
| 005 | 20241101091310.0 | ||
| 008 | 190411s1998 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0031803090 | |
| 024 | 7 | |2 cas |a Glycopeptides; Mannose, 31103-86-3; Oligosaccharides | |
| 030 | |a GLJOE | ||
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 100 | 1 | |a Lopes, C.H.G.L. | |
| 245 | 1 | 0 | |a Isolation, partial characterization and biological activity of mannosyl glycopeptides from seminal plasma |
| 260 | |c 1998 | ||
| 270 | 1 | 0 | |m Brandelli, A.; ICTA-UFRGS, Av. Bento Goncalves 9500, 91501-970 Porto Alegre, Brazil; email: abrand@vortex.ufrgs.br |
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| 504 | |a Tesarik, J., Mendoza, C., Carreras, A., (1991) Fertil Steril, 56, pp. 113-118 | ||
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| 504 | |a Sharon, N., Lis, H., (1989) Science, 246, pp. 227-234 | ||
| 506 | |2 openaire |e Política editorial | ||
| 520 | 3 | |a Affinity chromatography on Concanavalin-A Sepharose, followed by gel filtration and hydrophobic interaction chromatography, permits the isolation of low molecular weight N-glycosidically linked oligomannosidic glycopeptides (MGp) from the autoproteolysis products of human seminal plasma. The monosaccharide composition of MGp showed only mannose, N-acetylglucosamine and a small amount of galactose. Structural studies were carried out by methylation analysis and chromium trioxide oxidation, and results were consistent with the structures accepted for high-mannose N-glycans. MGp was capable of inhibiting the sperm acrosomal exocytosis mediated by sperm- surface receptors. These data suggest that MGp act as a 'decapacitation' factor preventing premature sperm exocytosis. |l eng | |
| 536 | |a Detalles de la financiación: Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: This work has received support from CNPq, Brasil, and CONICET, Argentina. | ||
| 593 | |a Departamento de Bioquímica, Instituto de Biociências, Univ. Federal do Rio Grande do Sul, 90050-170 Porto Alegre, Brazil | ||
| 593 | |a Depto. de Química Organica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina | ||
| 593 | |a Depto. de Cie. dos Alimentos, ICTA, Univ. Federal do Rio Grande do Sul, 91501 970 Porto Alegre, Brazil | ||
| 593 | |a ICTA-UFRGS, Av. Bento Gonçalves 9500, 91501-970 Porto Alegre, Brazil | ||
| 650 | 1 | 7 | |2 spines |a SEMEN |
| 650 | 1 | 7 | |2 spines |a SEMEN |
| 690 | 1 | 0 | |a CARBOHYDRATE |
| 690 | 1 | 0 | |a EXOCYTOSIS |
| 690 | 1 | 0 | |a GLYCOCONJUGATE |
| 690 | 1 | 0 | |a HUMAN |
| 690 | 1 | 0 | |a SPERM |
| 690 | 1 | 0 | |a GLYCOPEPTIDE |
| 690 | 1 | 0 | |a MANNOSE |
| 690 | 1 | 0 | |a ACROSOME |
| 690 | 1 | 0 | |a AFFINITY CHROMATOGRAPHY |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CARBOHYDRATE ANALYSIS |
| 690 | 1 | 0 | |a EXOCYTOSIS |
| 690 | 1 | 0 | |a HUMAN |
| 690 | 1 | 0 | |a MALE |
| 690 | 1 | 0 | |a NORMAL HUMAN |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a SEMINAL PLASMA |
| 690 | 1 | 0 | |a ACROSOME REACTION |
| 690 | 1 | 0 | |a CARBOHYDRATE CONFORMATION |
| 690 | 1 | 0 | |a CHROMATOGRAPHY, AFFINITY |
| 690 | 1 | 0 | |a CHROMATOGRAPHY, GAS |
| 690 | 1 | 0 | |a CHROMATOGRAPHY, GEL |
| 690 | 1 | 0 | |a EXOCYTOSIS |
| 690 | 1 | 0 | |a GLYCOPEPTIDES |
| 690 | 1 | 0 | |a HUMANS |
| 690 | 1 | 0 | |a MALE |
| 690 | 1 | 0 | |a MANNOSE |
| 690 | 1 | 0 | |a OLIGOSACCHARIDES |
| 700 | 1 | |a Mazzini, M.N. | |
| 700 | 1 | |a Tortorella, H. | |
| 700 | 1 | |a Konrath, R.A. | |
| 700 | 1 | |a Brandelli, Adriano | |
| 773 | 0 | |d 1998 |g v. 15 |h pp. 477-481 |k n. 5 |p Glycoconjugate J. |x 02820080 |w (AR-BaUEN)CENRE-581 |t Glycoconjugate Journal | |
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