Stabilizing crude and ultrafiltrated α-amylase and α-glucosidase from Aspergillus oryzae by trehalose
Thermal resistance of freeze-dried α-amylase and α-glucosidase in trehalose matrices (1 to 20 % w/v) stored at 90 °C and relative humidities (RH) between 0 and 44 % was studied. At RH values up to 33 %, 10 % (w/v) trehalose was necessary to retain at least 50 % of α-amylase activity. For α-glucosida...
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| Otros Autores: | , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1998
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 04777caa a22006737a 4500 | ||
|---|---|---|---|
| 001 | PAPER-2853 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203219.0 | ||
| 008 | 190411s1998 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0031715787 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a BTECE | ||
| 100 | 1 | |a Terebiznik, M.R. | |
| 245 | 1 | 0 | |a Stabilizing crude and ultrafiltrated α-amylase and α-glucosidase from Aspergillus oryzae by trehalose |
| 260 | |c 1998 | ||
| 270 | 1 | 0 | |m Terebiznik, M.R.; Dto. de Industrias FCEyN, Dto. de Ingenieria Quimica, Fl, Universidad de Buenos Aires C., Buenos Aires (1428), Argentina; email: mauricio@di.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Aguilera, J.M., Karel, M., (1997) Critical Rev Food Sci Nutr, 37, pp. 287-309 | ||
| 504 | |a Bergmeyer, H.U., Grassl, M., Reagents for enzymatic analysis: Enzymes α-glucosidase (1983) Methods of Enzymatic Analysis, 2, pp. 205-206. , HU Bergmeyer ed 3rd. edition Verlag Chemie GmbH, Weinheim | ||
| 504 | |a Cardona, S., Schebor, C., Buera, M., Karel, M., Chirife, J., (1997) J Food Sci, 62, pp. 105-112 | ||
| 504 | |a Colaco, C., Sen, S., Thangavelu, M., Pinder, S., Roser, B., (1992) Bio/technology, 10, pp. 1007-1011 | ||
| 504 | |a Karmas, R., Buera, M.P., Karel, M., (1992) J Agric Food Chem, 1, pp. 873-879 | ||
| 504 | |a Mazzobre, M.F., Buera, M.P., Chirife, J., (1997) Lebensm-Wiss-Technol, 30, pp. 324-339 | ||
| 504 | |a Rossi, S., Buera, M.P., Moreno, S., Chirife, J., (1998) Biotechnol Prog, 13, pp. 609-616 | ||
| 504 | |a Schebor, C., Buera, M.P., Chirife, J., (1996) J Food Eng, 30, pp. 269-282 | ||
| 504 | |a Scher, M., (1993) Food Processing, pp. 325-326. , April | ||
| 504 | |a Smith, B.W., Roe, J.H., (1949) J Biol Chem, 179, pp. 53-58 | ||
| 504 | |a Terebiznik, M.R., Pilosof, A.M.R., Moreno, S., (1996) J Food Biochem, 17, pp. 341-354 | ||
| 504 | |a Terebiznik, M.R., Buera, M.P., Pilosof, A.M.R., (1997) Lebensm-Wiss-Technol, 30, pp. 513-518 | ||
| 504 | |a Terebiznik, M.R., Buera, M.P., Pilosof, A.M.R., (1998) Lebensm-Wiss-Technol, 31, pp. 143-149 | ||
| 520 | 3 | |a Thermal resistance of freeze-dried α-amylase and α-glucosidase in trehalose matrices (1 to 20 % w/v) stored at 90 °C and relative humidities (RH) between 0 and 44 % was studied. At RH values up to 33 %, 10 % (w/v) trehalose was necessary to retain at least 50 % of α-amylase activity. For α-glucosidase, 10 % (w/v) trehalose was effective only at 0 % RH. Ultrafiltration of the crude enzymatic fermentation extracts enhanced enzyme stability per se. However, ultrafiltration in combination with 1% (w/v) trehalose retained 74 % of α-glucosidase and 95 % of α-amylase activities. |l eng | |
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires | ||
| 536 | |a Detalles de la financiación: International Foundation for Science | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: The authors acknowledge financial support from the University of Buenos Aires, International Foundation for Science (Sweden), Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina and Agencia Nacional de Promociones Científicas y Tecnológicas de la República Argentina. | ||
| 593 | |a Dto. de Industrias, FCEyN, C. Universitaria, (1428) Buenos Aires, Argentina | ||
| 593 | |a Dto. de Ingeniería Química, Fl. Universidad de Buenos Aires, C. Universitaria, (1428) Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a ALPHA GLUCOSIDASE |
| 690 | 1 | 0 | |a AMYLASE |
| 690 | 1 | 0 | |a ENZYME STABILITY |
| 690 | 1 | 0 | |a EXTRACTIVE FERMENTATION |
| 690 | 1 | 0 | |a FREEZE DRYING |
| 690 | 1 | 0 | |a HEAT RESISTANCE |
| 690 | 1 | 0 | |a HUMIDITY |
| 690 | 1 | 0 | |a MICROBE METABOLISM |
| 690 | 1 | 0 | |a TREHALOSE |
| 690 | 1 | 0 | |a ULTRAFILTRATION |
| 690 | 1 | 0 | |a ASPERGILLUS ORYZAE |
| 700 | 1 | |a Zylberman, V. | |
| 700 | 1 | |a Buera, M.P. | |
| 700 | 1 | |a Pilosof, A.M.R. | |
| 773 | 0 | |d 1998 |g v. 12 |h pp. 683-687 |k n. 9 |p Biotechnol. Tech. |x 0951208X |w (AR-BaUEN)CENRE-1441 |t Biotechnology Techniques | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031715787&doi=10.1023%2fA%3a1008860819947&partnerID=40&md5=93209988233239ebff7c179644aa2a27 |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1023/A:1008860819947 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_0951208X_v12_n9_p683_Terebiznik |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0951208X_v12_n9_p683_Terebiznik |y Registro en la Biblioteca Digital |
| 961 | |a paper_0951208X_v12_n9_p683_Terebiznik |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 63806 | ||