BAPTA-AM blocks both voltage-gated and Ca2+-activated K+ currents in cultured bovine chromaffin cells
The effects of the membrane permeant Ca2+ chelator BAPTA-AM on voltage-gated Na+, Ca2+, K+ (I(Na), I(Ca), I(K), respectively) and Ca2+-activated K+ (I(KCa)) currents in cultured bovine chromaffin cells were investigated using the whole-cell patch-clamp technique. Superfusion with BAPTA-AM (50 μM) in...
Guardado en:
| Autor principal: | |
|---|---|
| Otros Autores: | |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
Lippincott Williams and Wilkins
1998
|
| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 05130caa a22007457a 4500 | ||
|---|---|---|---|
| 001 | PAPER-2850 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203219.0 | ||
| 008 | 190411s1998 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0032569613 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a NERPE | ||
| 100 | 1 | |a Urbano, F.J. | |
| 245 | 1 | 0 | |a BAPTA-AM blocks both voltage-gated and Ca2+-activated K+ currents in cultured bovine chromaffin cells |
| 260 | |b Lippincott Williams and Wilkins |c 1998 | ||
| 270 | 1 | 0 | |m Urbano, F.J.; Lab. de Fisiologia y Biologia Molec., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428-Buenos Aires, Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Uneyama, H., Ueno, S., Akaike, N.J., (1993) Neurophysiol, 69, pp. 1044-1196 | ||
| 504 | |a Hunt, J.M., Redman, R.S., Silinky, E.M., (1994) Br J Pharmacol, 111, pp. 753-758 | ||
| 504 | |a Linden, D.J., Smeyne, M., Connor, J.A., (1994) J Neurophysiol, 71, pp. 1992-1998 | ||
| 504 | |a Chiamvimonvat, N., Kargacin, M.E., Clark, R.B., Duff, H.J., (1995) J Physiol (Lond), 483, pp. 307-318 | ||
| 504 | |a Tsien, R.Y., (1981) Nature, 290, pp. 527-528 | ||
| 504 | |a Niesen, C., Charlton, M.P., Carlen, P.L., (1991) Brain Res, 555, pp. 319-325 | ||
| 504 | |a Tymianski, M., Charlton, M.P., Carlen, P.L., Tator, C.H., (1994) J Neurophysiol, 72, pp. 1973-1992 | ||
| 504 | |a Tymianski, M., Spigelman, I., Zhang, L., (1994) J Cerebr Blood Flow Metab, 14, pp. 911-923 | ||
| 504 | |a Tymianski, M., Wallace, M.C., Spigelman, I., (1993) Neuron, 11, pp. 221-235 | ||
| 504 | |a Watkins, C.S., Mathie, A., (1996) Br J Pharmacology, 118, pp. 1772-1778 | ||
| 504 | |a Fenwick, E.M., Marty, A., Neher, E., (1982) J Physiol (Lond), 331, pp. 599-635 | ||
| 504 | |a Marty, A., Neher, E., (1985) J Physiol (Lond), 367, pp. 117-141 | ||
| 504 | |a Araque, A., Urbano, F.J., Cervenansky, C., (1995) J Neurosci Res, 42, pp. 539-546 | ||
| 504 | |a Moro, M.A., Lopez, M.G., Gandia, L., (1990) Anal Biochem, 185, pp. 243-248 | ||
| 504 | |a Hamill, O.P., Marty, A., Neher, E., (1981) Pflügers Arch, 391, pp. 85-100 | ||
| 504 | |a Araque, A., Buno, W., (1995) Pflügers Arch, 430, pp. 541-551 | ||
| 504 | |a Winslow, J.L., Duffy, S.N., Charlton, M.P., (1994) J Neurophysiol, 72, pp. 1769-1793 | ||
| 504 | |a Robitaille, R., Charlton, M.P., (1992) J Neurosci, 12, pp. 297-305 | ||
| 504 | |a Hille, B., (1992) Ionic Channels of Excitable Membranes, 2nd Edn., , Sunderland, MA: Sinauer Associates Inc | ||
| 504 | |a Valenzuela, C., Delpon, E., Tamkun, M.M., (1995) Biophys J, 69, pp. 418-427 | ||
| 504 | |a Cambray-Deakin, M.A., Burgoyne, R.D., (1992) Dev Brain Res, 66, pp. 25-32 | ||
| 520 | 3 | |a The effects of the membrane permeant Ca2+ chelator BAPTA-AM on voltage-gated Na+, Ca2+, K+ (I(Na), I(Ca), I(K), respectively) and Ca2+-activated K+ (I(KCa)) currents in cultured bovine chromaffin cells were investigated using the whole-cell patch-clamp technique. Superfusion with BAPTA-AM (50 μM) induced a rapid (< 60 s) and reversible block of both I(KCa) and I(K) (~50%), without affecting either I(Ca) or I(Na). Preincubation with BAPTA-AM (50 μM, 30 min) or cell loading with the non- permeable active form of BAPTA (10 mM in the pipette solution) permanently blocked I(KCa). BAPTA-AM superfusion (50 μM) also blocked I(K) (~53%) after BAPTA-loading or BAPTA-AM preincubation. In conclusion, we show a fast and reversible block of I(KCa) and I(K) by BAPTA-AM, acting directly on K+ channels before it operates as a Ca2+ chelator, in cultured bovine chromaffin cells. |l eng | |
| 593 | |a Inst. Santiago Ramón y Cajal, CSIC, Avenida Doctor Arce 37, 28002-Madrid, Spain | ||
| 593 | |a Lab. de Fisiol. y Biol. Molecular, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428-Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a BAPTA |
| 690 | 1 | 0 | |a BAPTA-AM |
| 690 | 1 | 0 | |a EXTRACELLULAR BLOCK |
| 690 | 1 | 0 | |a OPEN CHANNEL BLOCK |
| 690 | 1 | 0 | |a POTASSIUM CURRENT BLOCK |
| 690 | 1 | 0 | |a ANIMAL CELL |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CALCIUM TRANSPORT |
| 690 | 1 | 0 | |a CATTLE |
| 690 | 1 | 0 | |a CHANNEL GATING |
| 690 | 1 | 0 | |a CHROMAFFIN CELL |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PATCH CLAMP |
| 690 | 1 | 0 | |a POTASSIUM CURRENT |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 700 | 1 | |a Buño, W. | |
| 773 | 0 | |d Lippincott Williams and Wilkins, 1998 |g v. 9 |h pp. 3403-3407 |k n. 15 |p NeuroReport |x 09594965 |w (AR-BaUEN)CENRE-6274 |t NeuroReport | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032569613&doi=10.1097%2f00001756-199810260-00013&partnerID=40&md5=89e45658459863ed4051c13cce60407c |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1097/00001756-199810260-00013 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_09594965_v9_n15_p3403_Urbano |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09594965_v9_n15_p3403_Urbano |y Registro en la Biblioteca Digital |
| 961 | |a paper_09594965_v9_n15_p3403_Urbano |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 63803 | ||