L-arginine uptake and L-phosphoarginine synthesis in Trypanosoma cruzi
A very specific L-arginine transporter showing high affinity has been characterized in Trypanosoma cruzi epimastigotes. Uptake was found to be dependent on L-arginine concentration and it was saturable. Values for maximum velocity and Km ranged between 48.1-57.5 pmol·min-1 per 3 x 107 cells and betw...
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Society of Protozoologists
1999
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| LEADER | 09274caa a22009257a 4500 | ||
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| 001 | PAPER-2560 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203159.0 | ||
| 008 | 190411s1999 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0033230875 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a JEMIE | ||
| 100 | 1 | |a Pereira, C.A. | |
| 245 | 1 | 0 | |a L-arginine uptake and L-phosphoarginine synthesis in Trypanosoma cruzi |
| 260 | |b Society of Protozoologists |c 1999 | ||
| 270 | 1 | 0 | |m Torres, H.N.; CONICET, Facultad de Cie. Exactas y Naturales, Universidad de Buenos Aires, Obligado 2490, 1428 Buenos Aires, Argentina; email: torres@proteus.dna.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Camargo, E.P., Silva, S., Roitman, I., De Souza, W., Jankevicius, J.V., Dollet, M., Enzymes of the ornithine-arginine metabolism in Trypanosomatids of genus Phytomonas (1987) J. Protozool., 34, pp. 439-441 | ||
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| 504 | |a Dixon, M., Webb, E.C., (1964) Enzymes, 2d Ed., pp. 67-70. , Longmans, Green & Co., London | ||
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| 504 | |a France, R.M., Cellers, D.S., Grossman, S.H., Purification, characterization, and hydrodynamic properties of arginine kinase from Gulf Shrimp (Penaeus aztecus) (1997) Arch. Biochem. Biophys., 345, pp. 73-78 | ||
| 504 | |a Goldberg, S.S., Pereira, A.A., Chiari, E., Mares-Guia, M., Gazzinelli, G., Comparative kinetics of arginine and lysine transport by epimastigotes and trypomastigotes from two strains of Trypanosoma cruzi (1976) J. Protozool., 23, pp. 179-186 | ||
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| 504 | |a Hunter, K.J., Le Quesne, S.A., Fairlamb, A.H., Identification and biosynthesis of N1, N9-bis(glutathionyl)aminopropyl-cadaverine (homotrypanothione) in Trypanosoma cruzi (1994) Eur. J. Biochem., 226, pp. 1019-1027 | ||
| 504 | |a Kandpal, M., Fouce, R.B., Pal, A., Guru, P.Y., Tekwani, B.L., Kinetics and molecular characteristics of arginine transport by Leishmania donovani promastigotes (1995) Mol. Biochem. Parasitol., 71, pp. 193-201 | ||
| 504 | |a Le Quesne, S.A., Fairlamb, A.L., Regulation of a high-affinity diamine transport system in Trypanosoma cruzi epimastigotes (1996) Biochem. J., 316, pp. 481-486 | ||
| 504 | |a Le Quesne, S.A., Fairlamb, A.H., Measurement of polyamine transport (1997) Methods in Molecular Biology, 79, pp. 149-156. , Morgan, D. (ed.), Humana Press Inc., Totowa, N. J | ||
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| 504 | |a McGivan, J.D., Pastor-Anglada, M., Regulatory and molecular aspects of mammalian amino acid transport (1994) Biochem. J., 299, pp. 321-334 | ||
| 504 | |a Morrison, J.F., Arginine kinase and other guanidino kinases (1973) The Enzymes, 8, pp. 457-486. , Boyer, P.D. (ed.), Academic Press, New York | ||
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| 504 | |a Paveto, C., Pereira, C., Espinosa, J., Montagna, A.E., Farber, M., Esteva, M., Flawiá, M.M., Torres, H.N., The nitric oxide pathway in Trypanosoma cruzi (1995) J. Biol. Chem., 270, pp. 16576-16579 | ||
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| 504 | |a Richards, D.E., Rega, A.F., Garrahan, P.J., Two classes of sites for ATP in the Ca2+-ATPase from human red cell membranes (1978) Biochim. Biophys. Acta., 511, pp. 194-201 | ||
| 504 | |a Tabor, C.W., Tabor, H., Polyamines (1984) Ann. Rev. Biochem., 53, pp. 749-790 | ||
| 504 | |a White, M.F., The transport of cationic amino acids across the plasma membrane of mammalian cells (1985) Biochim. Biophys. Acta, 822, pp. 355-374 | ||
| 504 | |a White, M.F., Christensen, H.N., Cationic amino acid transport into culture animal cells. II Transport system barely perceptible in ordinary hepatocytes, but active in hepatoma cell lines (1982) J. Biol. Chem., 257, pp. 4450-4457 | ||
| 504 | |a Yoshida, N., Camargo, E.P., Urotelism and ammonotelism in trypanosomatids (1978) J. Bacteriol., 136, pp. 1184-1185 | ||
| 504 | |a Yu, L., Fengaro, P.E., Niederberg, M., Burke, T.J., Schrier, R.W., Nitric oxide: A mediator in rat tubular hypoxia/reoxygenation injury (1994) Proc. Natl. Acad. Sci. USA, 91, pp. 1691-1695 | ||
| 520 | 3 | |a A very specific L-arginine transporter showing high affinity has been characterized in Trypanosoma cruzi epimastigotes. Uptake was found to be dependent on L-arginine concentration and it was saturable. Values for maximum velocity and Km ranged between 48.1-57.5 pmol·min-1 per 3 x 107 cells and between 4.2-5.5 μM, respectively The calculated activation energy and Q10 were 31.1 KJ·mol-1, and 1.7, respectively. Uptake velocity significantly increased when cells were preincubated in the absence of L-arginine. Cells retained the labeled amino acid independently of the presence or absence of exogenous L-arginine. The specificity of L-arginine uptake was demonstrated by competition assays in the presence of 80-fold molar excess of natural amino acids and several L-arginine derivatives. The highest levels of inhibition were caused by L-homoarginine. D-arginine, L-canavanine, L-ornithine, and L-citrulline, L-arginine uptake by T. cruzi epimastigotes was not affected by the presence of potassium or sodium ions in the incubation mixture or by pH changes in the range between 5.5-8.5. The major product of L-arginine uptake was characterized as phosphoarginine. Moreover, arginine kinase activity was detected in soluble extracts from T. cruzi epimastigotes. |l eng | |
| 593 | |a Inst. Invest. Ing. Genetica y B., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Obligado 2490, 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a AMINO ACID TRANSPORT |
| 690 | 1 | 0 | |a ARGININE KINASE |
| 690 | 1 | 0 | |a CANAVANINE |
| 690 | 1 | 0 | |a EPIMASTIGOTES |
| 690 | 1 | 0 | |a HOMOARGININE |
| 690 | 1 | 0 | |a PHOSPHAGEN |
| 690 | 1 | 0 | |a AMINO ACID SYNTHESIS |
| 690 | 1 | 0 | |a AMINO ACID TRANSPORT |
| 690 | 1 | 0 | |a ARGININE |
| 690 | 1 | 0 | |a CANAVANINE |
| 690 | 1 | 0 | |a CITRULLINE |
| 690 | 1 | 0 | |a ENERGY METABOLISM |
| 690 | 1 | 0 | |a HOMOARGININE |
| 690 | 1 | 0 | |a MICROBE METABOLISM |
| 690 | 1 | 0 | |a ORNITHINE |
| 690 | 1 | 0 | |a PHOSPHOARGININE |
| 690 | 1 | 0 | |a TRYPANOSOMA |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 700 | 1 | |a Alonso, G.D. | |
| 700 | 1 | |a Paveto, M.C. | |
| 700 | 1 | |a Flawiá, M.M. | |
| 700 | 1 | |a Torres, H.N. | |
| 773 | 0 | |d Society of Protozoologists, 1999 |g v. 46 |h pp. 566-570 |k n. 6 |p J. Eukaryotic Microbiol. |x 10665234 |t Journal of Eukaryotic Microbiology | |
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| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_10665234_v46_n6_p566_Pereira |y Handle |
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| 999 | |c 63513 | ||