HEp-2 cell line as an experimental model to evaluate genotoxic effects of pentavalent inorganic arsenic
Early detection of toxic events induced by xenobiotics is necessary for a proper assessment of human risk after the exposure to those agents. The aim of this work was to evaluate the cell line HEp-2 as an experimental model to determine the genotoxic effects of sodium arsenate. To this end, we deter...
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Sociedad Argentina de Genetica
2017
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| LEADER | 15779caa a22012377a 4500 | ||
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| 001 | PAPER-25388 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518205725.0 | ||
| 008 | 190410s2017 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-85057638265 | |
| 024 | 7 | |2 cas |a arsenate sodium, 7631-89-2; glutathione, 70-18-8; glutathione transferase, 50812-37-8 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a BAGAB | ||
| 100 | 1 | |a Andrioli, N.B. | |
| 245 | 1 | 0 | |a HEp-2 cell line as an experimental model to evaluate genotoxic effects of pentavalent inorganic arsenic |
| 260 | |b Sociedad Argentina de Genetica |c 2017 | ||
| 270 | 1 | 0 | |m Andrioli, N.B.; Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Ecología, Genética y Evolución, Grupo de Investigación en Biología Evolutiva (GIBE), CONICET-Universidad de Buenos Aires, Instituto Instituto de Ecología, Genética y Evolución de Buenos Aires (IEGEBA)Argentina; email: nancyandrioli@gmail.com |
| 506 | |2 openaire |e Política editorial | ||
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| 520 | 3 | |a Early detection of toxic events induced by xenobiotics is necessary for a proper assessment of human risk after the exposure to those agents. The aim of this work was to evaluate the cell line HEp-2 as an experimental model to determine the genotoxic effects of sodium arsenate. To this end, we determined the metabolic activity cells by the MTT test on seven concentrations of arsenate that range from 27 to 135,000 μM, obtaining the median lethal concentration (LC50), the lowest observed effect concentration (LOEC), and the not observed effect concentration (NOEC) of sodium arsenate at 24 h of exposition. According to the cytotoxic response obtained, we evaluated the genotoxic effect of the 27 and 270 μM concentrations by using the micronucleus assay and chromosomal aberrations test. We found a statistically significant increase (p<0.05) in the frequency of micronuclei between control cultures and those exposed to the highest concentration of sodium arsenate. Furthermore, the frequencies of nucleoplasmic bridges and tripolar mitosis were significantly higher in cell cultures exposed to the above concentrations compared to the control cultures (p<0.05). The participation of the glutathione system as response to the arsenate exposition was also analyzed, and a statistically significant increase in the glutathione content was found in those cells exposed to 27 μM of arsenate. The Glutathione S-transferase activity did not increase in the exposed cells compared to control cells, suggesting that the arsenate reduction involved other metabolic pathways in the HEp-2 cells. These results confirm that, under the conditions carried out in this study, sodium arsenate is genotoxic for HEp-2 cells. Therefore, we suggest that this cell line would be a good model for the assessment of the cytotoxic and genotoxic effects of xenobiotics on human cells. © 2017 Sociedad Argentina de Genetica. All rights reserved. |l eng | |
| 593 | |a Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Ecología, Genética y Evolución, Grupo de Investigación en Biología Evolutiva (GIBE), CONICET-Universidad de Buenos Aires, Instituto Instituto de Ecología, Genética y Evolución de Buenos Aires (IEGEBA), Buenos Aires, Argentina | ||
| 593 | |a Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Laboratorio de Enzimología, Estrés y Metabolismo (LEEM), CONICET-Universidad de Buenos Aires, Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a CYTOTOXICITY |
| 690 | 1 | 0 | |a GENOTOXICITY |
| 690 | 1 | 0 | |a GLUTATHIONE |
| 690 | 1 | 0 | |a HEP-2 CELL LINE |
| 690 | 1 | 0 | |a ARSENATE SODIUM |
| 690 | 1 | 0 | |a GLUTATHIONE |
| 690 | 1 | 0 | |a GLUTATHIONE TRANSFERASE |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CHROMOSOME ABERRATION |
| 690 | 1 | 0 | |a CONCENTRATION (PARAMETERS) |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a CYTOTOXICITY |
| 690 | 1 | 0 | |a ENZYME ACTIVITY |
| 690 | 1 | 0 | |a EVALUATION STUDY |
| 690 | 1 | 0 | |a EXPERIMENTAL MODEL |
| 690 | 1 | 0 | |a GENOTOXICITY |
| 690 | 1 | 0 | |a HEP-2 CELL LINE |
| 690 | 1 | 0 | |a HUMAN |
| 690 | 1 | 0 | |a HUMAN CELL |
| 690 | 1 | 0 | |a LC50 |
| 690 | 1 | 0 | |a LOWEST OBSERVED EFFECT CONCENTRATION |
| 690 | 1 | 0 | |a MICRONUCLEUS |
| 690 | 1 | 0 | |a MICRONUCLEUS TEST |
| 690 | 1 | 0 | |a MTT ASSAY |
| 700 | 1 | |a Chaufan, G. | |
| 700 | 1 | |a Coalova, I. | |
| 700 | 1 | |a Ríos De Molina, M.C. | |
| 700 | 1 | |a Mudry, M.D. | |
| 773 | 0 | |d Sociedad Argentina de Genetica, 2017 |g v. 28 |h pp. 15-24 |k n. 2 |p BAG J. Basic Appl. Genet. |x 16660390 |w (AR-BaUEN)CENRE-9490 |t BAG - Journal of Basic and Applied Genetics | |
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