VIP induces the decidualization program and conditions the immunoregulation of the implantation process

The decidualization process involves phenotype and functional changes on endometrial cells and the modulation of mediators with immunoregulatory properties as the vasoactive intestinal peptide (VIP). We investigate VIP contribution to the decidualization program and to immunoregulation throughout th...

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Autor principal: Grasso, E.
Otros Autores: Gori, S., Paparini, D., Soczewski, E., Fernández, L., Gallino, L., Salamone, G., Martinez, G., Irigoyen, M., Ruhlmann, C., Pérez Leirós, C., Ramhorst, R.
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Elsevier Ireland Ltd 2018
Acceso en línea:Registro en Scopus
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LEADER 17314caa a22019097a 4500
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024 7 |2 scopus  |a 2-s2.0-85023634349 
024 7 |2 cas  |a dibutyrylcytidine 3',5' phosphate, 64649-87-2; interleukin 8, 114308-91-7; medroxyprogesterone, 520-85-4; prolactin, 12585-34-1, 50647-00-2, 9002-62-4; vasoactive intestinal polypeptide, 37221-79-7; Biomarkers; Culture Media, Conditioned; Vasoactive Intestinal Peptide 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a MCEND 
100 1 |a Grasso, E. 
245 1 0 |a VIP induces the decidualization program and conditions the immunoregulation of the implantation process 
260 |b Elsevier Ireland Ltd  |c 2018 
270 1 0 |m Ramhorst, R.; Laboratory of Immunopharmacology, School of Sciences, University of Buenos Aires, IQUIBICEN-CONICET, Int. Guiraldes 2160, Ciudad Universitaria, Pabellón 2 Piso 4, Argentina; email: rramhorst@qb.fcen.uba.ar 
506 |2 openaire  |e Política editorial 
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520 3 |a The decidualization process involves phenotype and functional changes on endometrial cells and the modulation of mediators with immunoregulatory properties as the vasoactive intestinal peptide (VIP). We investigate VIP contribution to the decidualization program and to immunoregulation throughout the human embryo implantation process. The decidualization of Human endometrial stromal cell line (HESC) with Medroxyprogesterone-dibutyryl-cAMP increased VIP/VPAC-receptors system. In fact, VIP could induce decidualization increasing differentiation markers (IGFBP1, PRL, KLF13/KLF9 ratio, CXCL12, CXCL8 and CCL2) and allowing Blastocyst-like spheroids (BLS) invasion in an in vitro model of embryo implantation. Focus on the tolerogenic effects, decidualized cells induced a semi-mature profile on maternal dendritic cells; restrained CD4+ cells recruitment while increased regulatory T-cells recruitment. Interestingly, the human blastocyst conditioned media from developmentally impaired embryos diminished the invasion and T-regulatory cells recruitment in these settings. These evidences suggest that VIP contributes to the implantation process inducing decidualization, allowing BLS invasion and favoring a tolerogenic micro-environment. © 2017 Elsevier B.V.  |l eng 
536 |a Detalles de la financiación: Universidad de Buenos Aires, UBACyT 20020130100040BA, 20020090200034 
536 |a Detalles de la financiación: National Council for Scientific Research, PIP 602/2012-2015 
536 |a Detalles de la financiación: PICT, 2013-1632, 2014-0657 
536 |a Detalles de la financiación: This work was funded by the National Agency of Sciences and Technology ANPCyT (PICT, 2013-1632 and 2014-0657), National Research Council CONICET (PIP 602/2012-2015) and University of Buenos Aires (UBACyT 20020130100040BA and 20020090200034). 
593 |a Immunopharmacology Laboratory, School of Sciences, University of Buenos Aires, IQUIBICEN-CONICET, Argentina 
593 |a Institute of Experimental Medicine IMEX-CONICET, National Academy of Sciences, Buenos Aires, Argentina 
593 |a Instituto de Fertilidad San Isidro, Buenos Aires, Argentina 
690 1 0 |a DECIDUALIZATION 
690 1 0 |a IMMUNOREGULATION 
690 1 0 |a IMPLANTATION 
690 1 0 |a VIP 
690 1 0 |a CYSTEINE RICH PROTEIN 61 
690 1 0 |a DIBUTYRYLCYTIDINE 3',5' PHOSPHATE 
690 1 0 |a INTERLEUKIN 8 
690 1 0 |a MEDROXYPROGESTERONE 
690 1 0 |a MEMBRANE PROTEIN 
690 1 0 |a MONOCYTE CHEMOTACTIC PROTEIN 1 
690 1 0 |a PROLACTIN 
690 1 0 |a PROTEIN KLF13 
690 1 0 |a STROMAL CELL DERIVED FACTOR 1 
690 1 0 |a UNCLASSIFIED DRUG 
690 1 0 |a VASOACTIVE INTESTINAL POLYPEPTIDE 
690 1 0 |a VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 
690 1 0 |a BIOLOGICAL MARKER 
690 1 0 |a VASOACTIVE INTESTINAL POLYPEPTIDE 
690 1 0 |a ARTICLE 
690 1 0 |a BLASTOCYST 
690 1 0 |a CD4+ T LYMPHOCYTE 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a DECIDUALIZATION 
690 1 0 |a DENDRITIC CELL 
690 1 0 |a HUMAN 
690 1 0 |a HUMAN CELL 
690 1 0 |a IMMUNOREGULATION 
690 1 0 |a IN VITRO STUDY 
690 1 0 |a NIDATION 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a PROTEIN EXPRESSION 
690 1 0 |a REGULATORY T LYMPHOCYTE 
690 1 0 |a STROMAL CELL LINE 
690 1 0 |a TUMOR SPHEROID 
690 1 0 |a BIOLOGICAL MODEL 
690 1 0 |a CELL LINE 
690 1 0 |a CONDITIONED MEDIUM 
690 1 0 |a CYTOLOGY 
690 1 0 |a DECIDUA 
690 1 0 |a DRUG EFFECT 
690 1 0 |a ENDOMETRIUM 
690 1 0 |a FEMALE 
690 1 0 |a IMMUNOLOGICAL TOLERANCE 
690 1 0 |a IMMUNOLOGY 
690 1 0 |a METABOLISM 
690 1 0 |a NIDATION 
690 1 0 |a PHARMACOLOGY 
690 1 0 |a STROMA CELL 
690 1 0 |a TROPHOBLAST 
690 1 0 |a TUMOR MICROENVIRONMENT 
690 1 0 |a BIOMARKERS 
690 1 0 |a BLASTOCYST 
690 1 0 |a CELL LINE 
690 1 0 |a CELLULAR MICROENVIRONMENT 
690 1 0 |a CULTURE MEDIA, CONDITIONED 
690 1 0 |a DECIDUA 
690 1 0 |a EMBRYO IMPLANTATION 
690 1 0 |a ENDOMETRIUM 
690 1 0 |a FEMALE 
690 1 0 |a HUMANS 
690 1 0 |a IMMUNE TOLERANCE 
690 1 0 |a MODELS, BIOLOGICAL 
690 1 0 |a STROMAL CELLS 
690 1 0 |a TROPHOBLASTS 
690 1 0 |a VASOACTIVE INTESTINAL PEPTIDE 
700 1 |a Gori, S. 
700 1 |a Paparini, D. 
700 1 |a Soczewski, E. 
700 1 |a Fernández, L. 
700 1 |a Gallino, L. 
700 1 |a Salamone, G. 
700 1 |a Martinez, G. 
700 1 |a Irigoyen, M. 
700 1 |a Ruhlmann, C. 
700 1 |a Pérez Leirós, C. 
700 1 |a Ramhorst, R. 
773 0 |d Elsevier Ireland Ltd, 2018  |g v. 460  |h pp. 63-72  |p Mol. Cell. Endocrinol.  |x 03037207  |w (AR-BaUEN)CENRE-6138  |t Molecular and Cellular Endocrinology 
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856 4 0 |u https://doi.org/10.1016/j.mce.2017.07.006  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_03037207_v460_n_p63_Grasso  |y Handle 
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