Characterization of nutrient status of Halamphora luciae (Bacillariophyceae) using matrix-assisted ultraviolet laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)
The effects of N and P depletion on the production and structural characterization of the cellular carbohydrate polymers of the estuarine diatom Halamphora luciae in batch culture were examined using matrix-assisted laser desorption-ionization time-of flight mass spectrometry (MALDI-TOF MS) compleme...
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2018
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| LEADER | 20138caa a22014657a 4500 | ||
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| 003 | AR-BaUEN | ||
| 005 | 20230518205709.0 | ||
| 008 | 190410s2018 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-85049651213 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a EJPHE | ||
| 100 | 1 | |a Daglio, Y. | |
| 245 | 1 | 0 | |a Characterization of nutrient status of Halamphora luciae (Bacillariophyceae) using matrix-assisted ultraviolet laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) |
| 260 | |b Taylor and Francis Ltd. |c 2018 | ||
| 270 | 1 | 0 | |m Erra-Balsells, R.; Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación en Hidratos de Carbono (CIHIDECAR), Facultad de Ciencias Exactas y Naturales Pabellón II, 3er P, Ciudad UniversitariaArgentina; email: erra@qo.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 520 | 3 | |a The effects of N and P depletion on the production and structural characterization of the cellular carbohydrate polymers of the estuarine diatom Halamphora luciae in batch culture were examined using matrix-assisted laser desorption-ionization time-of flight mass spectrometry (MALDI-TOF MS) complemented with monosaccharide composition determination and structural analyses by methylation of aqueous extracted product. The MALDI MS analysis of the cells showed a similar profile in control and N- and P-depleted media, with a displacement to higher molecular weight for cells grown in depleted media. In the monosaccharide analyses, both nutrient depletion and culture ageing led to an increase in glucose content, indicating that MALDI-TOF MS in whole cells was detecting the changes in chrysolaminarin. The maxima for the ions from f/2-P and to a lesser extent in f/2-N were displaced to higher m/z values indicating a higher degree of polymerization (DP). Methylation analysis confirmed the presence of chrysolaminarin, a (1→3)-β-d-glucan with branching in C2 and C6, where the glucan backbone had a substitution every four glucose residues. The (1→3)-β-d-glucan was also detected in the cingule by fluorescence with aniline blue. © 2018, © 2018 British Phycological Society. |l eng | |
| 536 | |a Detalles de la financiación: National Council for Scientific Research | ||
| 536 | |a Detalles de la financiación: Agencia Nacional de Promoción Científica y Tecnológica, PME 125 | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: 0055BA] and CONICET [PIP 11220080100234, PIP 11220110100208 and PIP 0072CO] and ANPCyT [PICT 2012-0888]. The MALDI Ultraflex II (Bruker) TOF/TOF mass spectrometer was supported by a grant from ANPCYT, PME 125 (CEQUIBIEM, FCEN, UBA). YD is a Research Fellow of the National Research Council of Argentina (CONICET) and MLS, MCM and REB are research members of the same institution. | ||
| 593 | |a Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación en Hidratos de Carbono (CIHIDECAR), Facultad de Ciencias Exactas y Naturales Pabellón II, 3er P, Ciudad Universitaria, Buenos Aires, 1428, Argentina | ||
| 593 | |a Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Orgánica, Pabellón II, 3er P., Ciudad Universitaria, Buenos Aires, 1428, Argentina | ||
| 593 | |a Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Biodiversidad y Biología Experimental, Ciudad Universitaria-Pabellón 2, Buenos Aires, C1428EGA, Argentina | ||
| 690 | 1 | 0 | |a CELLULAR CARBOHYDRATE POLYMERS |
| 690 | 1 | 0 | |a CHRYSOLAMINARIN |
| 690 | 1 | 0 | |a DIATOM CELLS |
| 690 | 1 | 0 | |a HALAMPHORA LUCIAE |
| 690 | 1 | 0 | |a MALDI-TOF MS |
| 690 | 1 | 0 | |a NUTRIENT STRESS |
| 690 | 1 | 0 | |a AQUEOUS SOLUTION |
| 690 | 1 | 0 | |a CARBOHYDRATE |
| 690 | 1 | 0 | |a CELL |
| 690 | 1 | 0 | |a DIATOM |
| 690 | 1 | 0 | |a GLUCOSE |
| 690 | 1 | 0 | |a MASS SPECTROMETRY |
| 690 | 1 | 0 | |a METHYLATION |
| 690 | 1 | 0 | |a MOLECULAR ANALYSIS |
| 690 | 1 | 0 | |a NITROGEN |
| 690 | 1 | 0 | |a NUTRIENT LOSS |
| 690 | 1 | 0 | |a PHOSPHORUS |
| 690 | 1 | 0 | |a POLYMER |
| 690 | 1 | 0 | |a POLYMERIZATION |
| 690 | 1 | 0 | |a POLYSACCHARIDE |
| 690 | 1 | 0 | |a BACILLARIOPHYCEAE |
| 690 | 1 | 0 | |a BACILLARIOPHYTA |
| 700 | 1 | |a Salum, M.L. | |
| 700 | 1 | |a Rodríguez, M.C. | |
| 700 | 1 | |a Erra-Balsells, R. | |
| 700 | 1 | |a Matulewicz, M.C. | |
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