POLAR-guided signalling complex assembly and localization drive asymmetric cell division

Stomatal cell lineage is an archetypal example of asymmetric cell division (ACD), which is necessary for plant survival 1–4 . In Arabidopsis thaliana, the GLYCOGEN SYNTHASE KINASE3 (GSK3)/SHAGGY-like kinase BRASSINOSTEROID INSENSITIVE 2 (BIN2) phosphorylates both the mitogen-activated protein kinase...

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Autor principal: Houbaert, A.
Otros Autores: Zhang, C., Tiwari, M., Wang, K., de Marcos Serrano, A., Savatin, D.V, Urs, M.J, Zhiponova, M.K, Gudesblat, G.E, Vanhoutte, I., Eeckhout, D., Boeren, S., Karimi, M., Betti, C., Jacobs, T., Fenoll, C., Mena, M., de Vries, S., De Jaeger, G., Russinova, E.
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Nature Publishing Group 2018
Acceso en línea:Registro en Scopus
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024 7 |2 cas  |a mitogen activated protein kinase, 142243-02-5; protein kinase, 9026-43-1 
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100 1 |a Houbaert, A. 
245 1 0 |a POLAR-guided signalling complex assembly and localization drive asymmetric cell division 
260 |b Nature Publishing Group  |c 2018 
270 1 0 |m Russinova, E.; Department of Plant Biotechnology and Bioinformatics, Ghent UniversityBelgium; email: eurus@psb.vib-ugent.be 
506 |2 openaire  |e Política editorial 
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520 3 |a Stomatal cell lineage is an archetypal example of asymmetric cell division (ACD), which is necessary for plant survival 1–4 . In Arabidopsis thaliana, the GLYCOGEN SYNTHASE KINASE3 (GSK3)/SHAGGY-like kinase BRASSINOSTEROID INSENSITIVE 2 (BIN2) phosphorylates both the mitogen-activated protein kinase (MAPK) signalling module 5,6 and its downstream target, the transcription factor SPEECHLESS (SPCH) 7 , to promote and restrict ACDs, respectively, in the same stomatal lineage cell. However, the mechanisms that balance these mutually exclusive activities remain unclear. Here we identify the plant-specific protein POLAR as a stomatal lineage scaffold for a subset of GSK3-like kinases that confines them to the cytosol and subsequently transiently polarizes them within the cell, together with BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE (BASL), before ACD. As a result, MAPK signalling is attenuated, enabling SPCH to drive ACD in the nucleus. Moreover, POLAR turnover requires phosphorylation on specific residues, mediated by GSK3. Our study reveals a mechanism by which the scaffolding protein POLAR ensures GSK3 substrate specificity, and could serve as a paradigm for understanding regulation of GSK3 in plants. © 2018, Springer Nature Limited.  |l eng 
593 |a Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium 
593 |a Center for Plant Systems Biology, VIB, Ghent, Belgium 
593 |a College of Life Sciences, Wuhan University, Wuhan, China 
593 |a Facultad de Ciencias Ambientales y Bioquíımica, Universidad de Castilla-la Mancha, Toledo, Spain 
593 |a Laboratory of Biochemistry, Wageningen University, Wageningen, Netherlands 
593 |a Department of Plant Physiology, Biological Faculty, University of Sofia, Sofia, Bulgaria 
593 |a Instituto de Biodiversidad y Biología Experimental y Aplicada, Departamento de Fisiología, Biología Molecular y Celular, Universidad de Buenos Aires, Buenos Aires, Argentina 
593 |a Department of Medicine, University of Perugia, Perugia, Italy 
690 1 0 |a BIN2 PROTEIN 
690 1 0 |a GLYCOGEN SYNTHASE KINASE 3 
690 1 0 |a MITOGEN ACTIVATED PROTEIN KINASE 
690 1 0 |a PLANT PROTEIN 
690 1 0 |a POLAR PROTEIN 
690 1 0 |a PROTEIN KINASE 
690 1 0 |a SPEECHLESS PROTEIN 
690 1 0 |a TRANSCRIPTION FACTOR 
690 1 0 |a UNCLASSIFIED DRUG 
690 1 0 |a ARABIDOPSIS THALIANA 
690 1 0 |a ASYMMETRIC CELL DIVISION 
690 1 0 |a CELL FATE 
690 1 0 |a CELL LINEAGE 
690 1 0 |a CELL NUCLEUS 
690 1 0 |a CELLULAR DISTRIBUTION 
690 1 0 |a COMPLEX FORMATION 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a CYTOSOL 
690 1 0 |a ENZYME SPECIFICITY 
690 1 0 |a LETTER 
690 1 0 |a MAPK SIGNALING 
690 1 0 |a NONHUMAN 
690 1 0 |a PLANT STOMA 
690 1 0 |a POLARIZATION 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a PROTEIN EXPRESSION 
690 1 0 |a PROTEIN PHOSPHORYLATION 
690 1 0 |a SIGNAL TRANSDUCTION 
700 1 |a Zhang, C. 
700 1 |a Tiwari, M. 
700 1 |a Wang, K. 
700 1 |a de Marcos Serrano, A. 
700 1 |a Savatin, D.V. 
700 1 |a Urs, M.J. 
700 1 |a Zhiponova, M.K. 
700 1 |a Gudesblat, G.E. 
700 1 |a Vanhoutte, I. 
700 1 |a Eeckhout, D. 
700 1 |a Boeren, S. 
700 1 |a Karimi, M. 
700 1 |a Betti, C. 
700 1 |a Jacobs, T. 
700 1 |a Fenoll, C. 
700 1 |a Mena, M. 
700 1 |a de Vries, S. 
700 1 |a De Jaeger, G. 
700 1 |a Russinova, E. 
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