Investigation of proteins in samples of a mid-18th century colonial mural painting by MALDI-TOF/MS and LC-ESI/MS (Orbitrap)

In this paper, we report the study of proteinaceous binders in samples from a South American Colonial 18th century painting using two complementary mass spectrometry techniques. Before restoration, seven micro-samples were extracted from representative colors of one of the mural paintings located in...

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Autor principal: Levy, I.K
Otros Autores: Neme Tauil, R., Valacco, M.P, Moreno, S., Siracusano, G., Maier, M.S
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Elsevier Inc. 2018
Acceso en línea:Registro en Scopus
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030 |a MICJA 
100 1 |a Levy, I.K. 
245 1 0 |a Investigation of proteins in samples of a mid-18th century colonial mural painting by MALDI-TOF/MS and LC-ESI/MS (Orbitrap) 
260 |b Elsevier Inc.  |c 2018 
270 1 0 |m Levy, I.K.; UMYMFOR-CONICET and Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, Argentina; email: ivanaklevy@gmail.com 
506 |2 openaire  |e Política editorial 
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504 |a Kuckova, S., Hynek, R., Kodicek, M., Identification of proteinaceous binders used in artworks by MALDI-TOF mass spectrometry (2007) Anal. Bioanal. Chem., 388, pp. 201-206 
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504 |a Calvano, C.D., van der Werf, I.D., Palmisano, F., Sabbatini, L., Identification of lipid- and protein-based binders in paintings by direct on-plate wet chemistry and matrix-assisted laser desorption ionization mass spectrometry (2015) Anal. Bioanal. Chem., 407, pp. 1015-1022 
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504 |a Fremout, W., Dhaenens, M., Saverwyns, S., Sanyova, J., Vandenabeele, P., Deforme, D., Moens, L., Tryptic peptide analysis of protein binders in works of art by liquid chromatography-tandem mass spectrometry (2010) Anal. Chim. Acta, 658, pp. 156-162 
504 |a Solazzo, C., Fitzhugh, W.W., Rolando, C., Tokarski, C., Identification of protein remains in archaeological potsherds by proteomics (2008) Anal. Chem., 80, pp. 4590-4597 
504 |a Kuckova, S., Anca Sandu, I.C., Crhova, M., Hynek, R., Fogas, I., Muralha, V.S., Sandu, A.V., Complementary cross-section-based protocol of investigation of polychrome samples of a 16th century Moravian sculpture by optical, vibrational and mass spectrometric techniques (2013) Microchem. J., 110, pp. 538-544 
504 |a van der Werf, I.D., Calvano, C.D., Palmisano, F., Sabbatini, L., A simple protocol for Matrix Assisted Laser Desorption Ionization- time of flight-mass spectrometry (MALDI-TOF-MS) analysis of lipids and proteins in single microsamples of paintings (2012) Anal. Chim. Acta, 718, pp. 1-10 
504 |a Tripković, T., Charvy, C., Alves, S., Lolić, A.Đ., Baošić, R.M., Nikolic-Mandić, S.D., Tabet, J.C., Electrospray ionization linear trap quadrupole Orbitrap in analysis of old tempera paintings: application to nineteenth-century orthodox icons (2015) Eur. J. Mass Spectrom., 21, pp. 679-692 
504 |a Andreotti, A., Bonaduce, I., Colombini, M.P., Gautier, G., Modugno, F., Ribechini, E., Combined GC/MS analytical procedure for the characterization of glycerolipid, waxy, resinous, and proteinaceous materials in a unique paint microsample (2006) Anal. Chem., 78, pp. 4490-4500 
504 |a Tomasini, E., Castellanos Rodríguez, D., Gómez, B.A., de Faria, D.L.A., Rúa Landa, C., Siracusano, G., Maier, M.S., A multi-analytical investigation of the materials and painting technique of a wall painting from the church of Copacabana de Andamarca (Bolivia) (2016) Microchem. J., 128, pp. 172-180 
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504 |a Fremout, W., Kuckova, S., Crhova, M., Sanyova, J., Saverwyns, S., Hynek, R., Kodicek, M., Moens, L., Classification of protein binders in artist's paints by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry: an evaluation of principal component analysis (PCA) and soft independent modelling of class analogy (SIMCA) (2011) Rapid Commun. Mass Spectrom., 25, pp. 1631-1640 
504 |a Pacheco, F., Arte de la pintura (1982), Las Ediciones de Arte Barcelona; Bella, J., Hulmes, D.J.S., Fibrillar collagens (2017) Fibrous Proteins: Structures and Mechanisms. Subcellular Biochemistry, 82. , D. Parry J. Squire Springer Cham 
520 3 |a In this paper, we report the study of proteinaceous binders in samples from a South American Colonial 18th century painting using two complementary mass spectrometry techniques. Before restoration, seven micro-samples were extracted from representative colors of one of the mural paintings located inside the church of Our Lady of Copacabana de Andamarca built in 1723 in Bolivia. Previous analysis by gas chromatography (GC) of the amino acid derivatives of the extracted protein fraction of three of the samples, suggested the presence of animal glue and egg. In this work, we introduce a methodology that combines a protein extraction procedure with a typical treatment for analysis of proteins as tryptic peptides by mass spectrometry. For this study, we applied in the first stage, MALDI-TOF/MS and then, LC-ESI/MS (Orbitrap), an ultrahigh resolution mass spectrometry, to achieve more reliability in the identification of the protein binders. By LC-ESI/MS (Orbitrap), we detected several peptides from egg white proteins, particularly ovalbumin, ovotransferrin and lysozyme, and egg yolk proteins, vitellogenin-2 and apolipoprotein B, with high confidence peptides each. In all the samples, the presence of collagen from animal provenance was established by MALDI-TOF/MS and LC-ESI/MS (Orbitrap). To our knowledge, this is the first time that protein binders in Andean paintings are identified without ambiguity by MALDI-TOF/MS and LC-ESI/MS (Orbitrap) mass spectrometry using a proteomic approach. © 2018 Elsevier B.V.  |l eng 
536 |a Detalles de la financiación: Universidad de Buenos Aires, 20020170100340BA, 20020130100008BA 
536 |a Detalles de la financiación: Agencia Nacional de Promoción Científica y Tecnológica, PICT-2016-0349 
536 |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas, PIP 11220130100288 
536 |a Detalles de la financiación: The authors are indebted to the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) ( PIP 11220130100288 ), Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) ( PICT-2016-0349 ), and the University of Buenos Aires ( 20020130100008BA and 20020170100340BA ), Argentina, for financial support. The authors are grateful to the Ministerio de Culturas y Turismo of Bolivia for their support. Authors acknowledge the Mass Spectrometry facilities of CEQUIBIEM (Centro de Estudios Químicos y Biológicos por Espectrometría de Masas). I.K.L., S.M., P.V., G.S., and M.S.M. are Research Members of CONICET. Authors would like to thank the reviewers for their comments and suggestions that helped improve the manuscript. 
593 |a UMYMFOR-CONICET and Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, Ciudad Autónoma de Buenos Aires, C1428EGA, Argentina 
593 |a CONICET, Godoy Cruz 2290, Ciudad Autónoma de Buenos Aires, C1425FQB, Argentina 
593 |a Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, Ciudad Autónoma de Buenos Aires, C1428EGA, Argentina 
593 |a Centro de Investigación en Arte, Materia y Cultura (IIAC), Universidad Nacional de Tres de Febrero, Avenida Antártida Argentina 1355, Ciudad Autónoma de Buenos Aires, C1104ACA, Argentina 
690 1 0 |a COLONIAL MURAL PAINTINGS 
690 1 0 |a EGG TEMPERA TECHNIQUE 
690 1 0 |a LC-ESI/MS (ORBITRAP) 
690 1 0 |a MALDI-TOF/MS 
690 1 0 |a PROTEINACEOUS BINDERS 
690 1 0 |a PROTEOMIC TOOLS 
700 1 |a Neme Tauil, R. 
700 1 |a Valacco, M.P. 
700 1 |a Moreno, S. 
700 1 |a Siracusano, G. 
700 1 |a Maier, M.S. 
773 0 |d Elsevier Inc., 2018  |g v. 143  |h pp. 457-466  |p Microchem. J.  |x 0026265X  |w (AR-BaUEN)CENRE-2274  |t Microchemical Journal 
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