Detection of Trypanosoma cruzi infection in naturally infected dogs and cats using serological, parasitological and molecular methods

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Domestic dogs and cats are major domestic reservoir hosts of Trypanosoma cruzi and a risk factor for parasite transmission. In this study we assessed the relative performance of a polymerase chain reaction assay targeted to minicircle DNA (kDNA-PCR) in reference to conventional serological tests, a...

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Autor principal: Enriquez, G.F
Otros Autores: Cardinal, M.V, Orozco, M.M, Schijman, A.G, Gürtler, Ricardo Esteban
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2013
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ARGENTINA
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Biblioteca Central Dr. Luis F. Leloir (FCEN) de Universidad de Buenos Aires
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100 1 |a Enriquez, G.F. 
245 1 0 |a Detection of Trypanosoma cruzi infection in naturally infected dogs and cats using serological, parasitological and molecular methods 
260 |c 2013 
270 1 0 |m Cardinal, M.V.; Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina; email: mvcardinal@ege.fcen.uba.ar 
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504 |a Gürtler, R.E., Cecere, M.C., Rubel, D.N., Petersen, R.M., Schweigmann, N.J., Lauricella, M.A., Bujas, M.A., Wisnivesky-Colli, C., Chagas disease in northwest Argentina: infected dogs as a risk factor for the domestic transmission of Trypanosoma cruzi (1991) Trans. R. Soc. Trop. Med. Hyg., 85, pp. 741-745 
504 |a Gürtler, R.E., Cecere, M.C., Castañera, M.B., Canale, D., Lauricella, M.A., Chuit, R., Cohen, J.E., Segura, E.L., Probability of infection with Trypanosoma cruzi of the vector Triatoma infestans fed on infected humans and dogs in northwest Argentina (1996) Am. J. Trop. Med. Hyg., 55, pp. 24-31 
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506 |2 openaire  |e Política editorial 
520 3 |a Domestic dogs and cats are major domestic reservoir hosts of Trypanosoma cruzi and a risk factor for parasite transmission. In this study we assessed the relative performance of a polymerase chain reaction assay targeted to minicircle DNA (kDNA-PCR) in reference to conventional serological tests, a rapid dipstick test and xenodiagnosis to detect T. cruzi infection in dogs and cats from an endemic rural area in northeastern Argentina. A total of 43 dogs and 13 cats seropositive for T. cruzi by an immunosorbent assay (ELISA) and an indirect hemagglutination assay (IHA), which had been examined by xenodiagnosis, were also tested by kDNA-PCR. kDNA-PCR was nearly as sensitive as xenodiagnosis for detecting T. cruzi-infectious dogs and cats. kDNA-PCR was slightly more sensitive than xenodiagnosis in seropositive dogs (91% versus 86%, respectively) and cats (77% against 54%, respectively), but failed to detect all of the seropositive individuals. ELISA and IHA detected all xenodiagnosis-positive dogs and both outcomes largely agreed (kappa coefficient, κ= 0.92), whereas both assays failed to detect all of the xenodiagnosis-positive cats and their agreement was moderate (κ= 0.68). In dogs, the sensitivity of the dipstick test was 95% and agreed closely with the outcome of conventional serological tests (κ= 0.82). The high sensitivity of kDNA-PCR to detect T. cruzi infections in naturally infected dogs and cats supports its application as a diagnostic tool complementary to serology and may replace the use of xenodiagnosis or hemoculture. © 2013 Elsevier B.V.  |l eng 
536 |a Detalles de la financiación: Universidad de Buenos Aires 
536 |a Detalles de la financiación: Fogarty International Center 
536 |a Detalles de la financiación: National Institute of Environmental Health Sciences 
536 |a Detalles de la financiación: R01 TW05836 
536 |a Detalles de la financiación: This study received financial support from International Development Research Center (Eco-Health Program); the UNICEF/UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR) ; the National Institutes of Health/National Science Foundation Ecology of Infectious Disease program award R01 TW05836 funded by the Fogarty International Center and the National Institute of Environmental Health Sciences (to Uriel Kitron and REG), and University of Buenos Aires . REG, MVC and AGS are members of CONICET Researcher's Career. 
593 |a Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, Buenos Aires, Argentina 
593 |a Laboratorio de Biología Molecular de la Enfermedad de Chagas, Instituto de Investigaciones en Ingeniería Genetica y Biologia Molecular (INGEBI-CONICET), Vuelta de Obligado 2490, Buenos Aires, Argentina 
651 4 |a ARGENTINA 
651 4 |a ARGENTINA 
651 4 |a ARGENTINA 
690 1 0 |a CHAGAS DISEASE 
690 1 0 |a POLYMERASE CHAIN REACTION 
690 1 0 |a RESERVOIR 
690 1 0 |a SERODIAGNOSIS 
690 1 0 |a TRYPANOSOMA CRUZI 
690 1 0 |a XENODIAGNOSIS 
690 1 0 |a MINICIRCLE DNA 
690 1 0 |a CANID 
690 1 0 |a DETECTION METHOD 
690 1 0 |a DISEASE INCIDENCE 
690 1 0 |a DNA 
690 1 0 |a FELID 
690 1 0 |a IMMUNOASSAY 
690 1 0 |a INFECTIVITY 
690 1 0 |a PARASITE TRANSMISSION 
690 1 0 |a POLYMERASE CHAIN REACTION 
690 1 0 |a RISK FACTOR 
690 1 0 |a RURAL AREA 
690 1 0 |a TRYPANOSOMIASIS 
690 1 0 |a ANIMAL EXPERIMENT 
690 1 0 |a ARTICLE 
690 1 0 |a CAT DISEASE 
690 1 0 |a CHAGAS DISEASE 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a CROSS-SECTIONAL STUDY 
690 1 0 |a DIAGNOSTIC TEST ACCURACY STUDY 
690 1 0 |a DOG DISEASE 
690 1 0 |a ENZYME LINKED IMMUNOSORBENT ASSAY 
690 1 0 |a HEMAGGLUTINATION TEST 
690 1 0 |a INDIRECT HEMAGGLUTINATION ASSAY 
690 1 0 |a INTERMETHOD COMPARISON 
690 1 0 |a MOLECULAR DIAGNOSIS 
690 1 0 |a NONHUMAN 
690 1 0 |a PARASITE SERODIAGNOSIS 
690 1 0 |a POLYMERASE CHAIN REACTION 
690 1 0 |a RAPID DIPSTICK TEST 
690 1 0 |a RURAL AREA 
690 1 0 |a SENSITIVITY ANALYSIS 
690 1 0 |a TRYPANOSOMA CRUZI 
690 1 0 |a XENODIAGNOSIS 
690 1 0 |a ANIMALS 
690 1 0 |a CAT DISEASES 
690 1 0 |a CATS 
690 1 0 |a CHAGAS DISEASE 
690 1 0 |a DIAGNOSTIC TESTS, ROUTINE 
690 1 0 |a DOG DISEASES 
690 1 0 |a DOGS 
690 1 0 |a MOLECULAR DIAGNOSTIC TECHNIQUES 
690 1 0 |a PARASITOLOGY 
690 1 0 |a SENSITIVITY AND SPECIFICITY 
690 1 0 |a SEROLOGIC TESTS 
690 1 0 |a TRYPANOSOMA CRUZI 
690 1 0 |a VETERINARY MEDICINE 
690 1 0 |a CANIS FAMILIARIS 
690 1 0 |a TRYPANOSOMA CRUZI 
700 1 |a Cardinal, M.V. 
700 1 |a Orozco, M.M. 
700 1 |a Schijman, A.G. 
700 1 |a Gürtler, Ricardo Esteban 
773 0 |d 2013  |g v. 126  |h pp. 211-217  |k n. 3  |p Acta Trop.  |x 0001706X  |w (AR-BaUEN)CENRE-3547  |t Acta Tropica 
856 4 1 |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-84875945363&doi=10.1016%2fj.actatropica.2013.03.001&partnerID=40&md5=6a5950bb329a649e53979ed2ed75d9a7  |y Registro en Scopus 
856 4 0 |u https://doi.org/10.1016/j.actatropica.2013.03.001  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_0001706X_v126_n3_p211_Enriquez  |y Handle 
856 4 0 |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0001706X_v126_n3_p211_Enriquez  |y Registro en la Biblioteca Digital 
961 |a paper_0001706X_v126_n3_p211_Enriquez  |b paper  |c PE 
962 |a info:eu-repo/semantics/article  |a info:ar-repo/semantics/artículo  |b info:eu-repo/semantics/publishedVersion 
963 |a VARI 
999 |c 85224 

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