The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase
The present work was carried out with the aim to analyze the secretome of Trametes versicolor BAFC 2234 grown on tomato juice medium supplemented with copper and manganese. T. versicolor BAFC 2234 was selected among diverse wood dwelling agaricomycetes from Argentina by its ability to cause a strong...
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| Formato: | Capítulo de libro |
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2013
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 19121caa a22019337a 4500 | ||
|---|---|---|---|
| 001 | PAPER-24167 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518205558.0 | ||
| 008 | 190411s2013 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-84883674268 | |
| 024 | 7 | |2 cas |a copper, 15158-11-9, 7440-50-8; hydrolase, 9027-41-2; laccase, 80498-15-3; lignocellulose, 11132-73-3; manganese, 16397-91-4, 7439-96-5; manganese peroxidase, 114995-15-2; monophenol monooxygenase, 9002-10-2; peroxidase, 9003-99-0 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a JBITD | ||
| 100 | 1 | |a Carabajal, M. | |
| 245 | 1 | 4 | |a The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase |
| 260 | |c 2013 | ||
| 270 | 1 | 0 | |m Levin, L.; University of Buenos Aires, Faculty of Exact and Natural Sciences, Laboratorio de Micología Experimental, Dept. of Biodiv. and Exp. Biology, Intendente Güiraldes 2160, Ciudad Univ., C1428EGA, Argentina; email: lale@bg.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Asgher, M., Bhatti, H.N., Ashraf, M., Legge, R.L., Recent developments in biodegradation of industrial pollutants by white rot fungi and their enzyme system (2008) Biodegradation, 19, pp. 771-783 | ||
| 504 | |a Asgher, M., Nasir Iqbal, H.M., Characterization of a novel manganese peroxidase purified from solid state culture of Trametes versicolor IBL-04 (2011) Bioresources, 6, pp. 4317-4330 | ||
| 504 | |a Baldrian, P., Fungal laccases - occurrence and properties (2006) FEMS Microbiol. Rev., 30, pp. 215-242 | ||
| 504 | |a Barreiro, C., García-Estrada, C., Martín, J.F., Proteomics methodology applied to the analysis of filamentous fungi - new trends for an impressive diverse group of organisms (2011) Tandem Mass Spectrometry - Applications and Principles, pp. 127-160. , InTech, Rijeka, Croatia, J.K. Prasain (Ed.) | ||
| 504 | |a Berrin, J.-G., Navarro, D., Couturier, M., Olivé, C., Grisel, S., Haon, M., Taussac, S., Lesage-Meessen, L., Exploring the natural fungal biodiversity of tropical and temperate forests toward improvement of biomass conversion (2012) Appl. Environ. Microbiol., 78, pp. 6483-6490 | ||
| 504 | |a Bourbonnais, R., Paice, M.G., Reid, I.D., Lanthier, P., Yaguchi, M., Lignin oxidation by laccase isozymes from Trametes versicolor and role of the mediator 2,2-azinobis (3-ethylbenzthiazoline-6-sulfonate) in kraft lignin depolymerization (1995) Appl. Environ. Microbiol., 61, pp. 1876-1880 | ||
| 504 | |a Camarero, S., Sarkar, S., Ruiz-Dueñas, F.J., Martinez, M.J., Martinez, A.T., Description of a versatile peroxidase involved in the natural degradation of lignin that has both manganese peroxidase and lignin peroxidase substrate interaction sites (1999) J. Biol. Chem., 274, pp. 10324-10330 | ||
| 504 | |a Carabajal, M., Ullrich, R., Levin, L., Kluge, M., Hofrichter, M., Degradation of phenol and p-nitrophenol by the white-rot polypore Trametes versicolor (2012) Proceedings Book of the 5th International Symposium on Biosorption and Bioremediation, pp. 69-72 | ||
| 504 | |a Chakroun, H., Bouaziz, M., Dhouib, A., Sayadi, S., Enzymatic oxidative transformation of phenols by Trametes trogii laccases (2012) Environ. Technol., 33, pp. 1977-1985 | ||
| 504 | |a Cullen, D., Kersten, P.J., Enzymology and molecular biology of lignin degradation (2004) The Mycota III: Biochemistry and Molecular Biology, pp. 249-273. , Springer-Verlag, Berlin, Heidelberg, R. Brambl, G.A. Marzluf (Eds.) | ||
| 504 | |a Floudas, D., Binder, M., Riley, R., Barry, K., Blanchette, R.A., Henrissat, B., Martinez, A.T., Hibbett, D.S., The Paleozoic origin of enzymatic lignin decomposition reconstructed from 31 fungal genomes (2012) Science, 336, pp. 1715-1719 | ||
| 504 | |a Gao, D., Du, L., Yang, J., Wu, W.M., Liang, H., A critical review of the application of white rot fungus to environmental pollution control (2010) Crit. Rev. Biotechnol., 30, pp. 70-77 | ||
| 504 | |a Gianazza, E., Isoelectric focusing as a tool for the investigation of posttranslational processing and chemical modifications of proteins (1995) J. Chromatogr., 705, pp. 67-87 | ||
| 504 | |a Grey, R., Höfer, C., Schlosser, D., Degradation of 2-chlorophenol and formation of 2-chloro-1,4-benzoquinone by mycelia and cell-free crude culture liquids of Trametes versicolor in relation to extracellular laccase activity (1998) J. Basic Microbiol., 38, pp. 371-382 | ||
| 504 | |a Hatakka, A., Lignin-modifying enzymes from selected white-rot fungi: production and role in lignin degradation (1994) FEMS Microbiol. Rev., 13, pp. 125-135 | ||
| 504 | |a Hatakka, A., Biodegradation of lignin (2001) Biopolymers, Volume 1: Lignin, Humic Substances and Coal, pp. 129-180. , Wiley-VCH, Weinheim, M. Hofrichter, A. Steinbuchel (Eds.) | ||
| 504 | |a Heinfling, A., Ruiz-Dueñas, F.J., Martinez, M.J., Bergbauer, M., Szewzyk, U., Martinez, A.T., A study on reducing substrates of manganese-oxidizing peroxidases from Pleurotus eryngii and Bjerkandera adusta (1998) FEBS Lett., 428, pp. 141-146 | ||
| 504 | |a Hertog, M.G.L., Hollman, P.C.H., Katan, M.B., Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9 fruits commonly consumed in The Netherlands (1992) J. Agric. Food Chem., 40, pp. 2379-2383 | ||
| 504 | |a Hofrichter, M., Lignin conversion by manganese peroxidase (MnP) (2002) Enzyme Microb. Technol., 30, pp. 454-466 | ||
| 504 | |a Hofrichter, M., Ullrich, R., Pecyna, M., Liers, C., Lundell, T., New and classic families of secreted fungal heme peroxidases (2010) Appl. Microbiol. Biotechnol., 87, pp. 871-897 | ||
| 504 | |a Ji, X.-L., Zhang, W.-T., Gai, Y.-P., Lu, B.-Y., Yuan, C.-Z., Liu, Q.-X., Mu, Z.-M., Patterns of lignocellulose degradation and secretome analysis of Trametes trogii MT (2012) Int. Biodeter. Biodegrad., 75, pp. 55-62 | ||
| 504 | |a Johansson, T., Nyman, P.O., Isozymes of lignin peroxidase and manganese (II) peroxidase from the white-rot basidiomycete Trametes versicolor. I. Isolation of enzyme forms and characterization of physical and catalytic properties (1993) Arch. Biochem. Biophys., 300, pp. 49-56 | ||
| 504 | |a Johansson, T., Welinder, K.G., Nyman, P.O., Isozymes of lignin peroxidase and manganese (II) peroxidase from the white-rot basidiomycete Trametes versicolor. II. Partial sequences, peptide maps, and amino acid and carbohydrate compositions (1993) Arch. Biochem. Biophys., 300, pp. 57-62 | ||
| 504 | |a Jönsson, L., Saloheimo, M., Penttilä, M., Laccase from the white-rot fungus Trametes versicolor: cDNA cloning of lcc1 and expression in Pichia pastoris (1997) Curr. Genet., 32, pp. 425-430 | ||
| 504 | |a Khachik, F., Beecher, G.R., Smith, J.C., Lutein, lycopene, and their oxidative metabolites in chemoprevention of cancer (1995) J. Cell. Biochem., 22, pp. 236-246 | ||
| 504 | |a Kirk, T.K., Croan, S., Tien, M., Murtagh, E., Farrell, R.L., Production of multiple ligninases by Phanerochaete chrysosporium: effect of selected growth conditions and use of mutant strain (1986) Enzyme Microb. Technol., 8, pp. 27-32 | ||
| 504 | |a Kim, Y., Yeo, S., Kum, J., Song, H., Choi, H., Cloning of a manganese peroxidase cDNA gene repressed by manganese in Trametes versicolor (2005) J. Microbiol., 43, pp. 569-571 | ||
| 504 | |a Kim, Y., Nandakumar, M.P., Marten, M.R., Proteomics of filamentous fungi (2007) Trends Biotechnol., 25, pp. 395-400 | ||
| 504 | |a Kulp, W.L., Scientific apparatus and laboratory methods an agar medium for plating L. acidophilus and L. bulgaricus (1927) Science, 25, pp. 512-513 | ||
| 504 | |a Kusters-van Someren, M., Kishi, K., Lundel, T., Gold, M.H., The manganese binding site of manganese peroxidase: characterization of an Asp 179Asn site-directed mutant protein (1995) Biochemistry, 34, pp. 10620-10627 | ||
| 504 | |a Leonowicz, A., Matuszewska, A., Luterek, J., Ziegenhagen, D., Wojtas-Wasilewska, M., Cho, N.S., Hofrichter, M., Rogalski, J., Biodegradation of lignin by white-rot fungi (1999) Fungal Genet. Biol., 27, pp. 175-185 | ||
| 504 | |a Levin, L., Forchiassin, F., Ligninolytic enzymes of the white rot basidiomycete Trametes trogii (2001) Acta Biotechnol., 21, pp. 179-186 | ||
| 504 | |a Liers, C., Ullrich, R., Pecyna, M., Schlosser, D., Hofrichter, M., Production, purification and partial enzymatic and molecular characterization of a laccase from the wood-rotting ascomycete Xylaria polymorpha (2007) Enzyme Microb. Technol., 41, pp. 785-793 | ||
| 504 | |a Liers, C., Bobeth, C., Pecyna, M., Ullrich, R., Hofrichter, M., DyP-like peroxidases of the jelly fungus Auricularia auricula-judae oxidize nonphenolic lignin model compounds and high-redox potential dyes (2010) Appl. Microb. Biotechnol., 85, pp. 1869-1879 | ||
| 504 | |a Lisov, A., Leontievsky, A., Golovleva, L., Hybrid Mn peroxidases from basidiomycetes (2007) Appl. Biochem. Microbiol., 43, pp. 536-543 | ||
| 504 | |a Lundell, T.K., Mäkelä, M.R., Hildén, K., Lignin-modifying enzymes in filamentous basidiomycetes - ecological, functional and phylogenetic review (2010) J. Basic Microbiol., 50, pp. 5-20 | ||
| 504 | |a Marco-Urrea, E., Perez-Trujillo, M., Caminal, G., Vicent, T., Dechlorination of 1,2,3 and 1,2,4-trichlorobenzene by the white-rot fungus Trametes versicolor (2009) J. Hazard. Mater., 166, pp. 1141-1147 | ||
| 504 | |a Martínez, A.T., Molecular biology and structure-function of lignin-degrading heme peroxidases (2002) Enzyme Microb. Technol., 30, pp. 425-444 | ||
| 504 | |a Martínez, A.T., Speranza, M., Ruiz-Dueñas, F.J., Ferreira, P., Camarero, S., Guillén, F., Martínez, M.J., del Río, J.C., Biodegradation of lignocellulosics: microbial, chemical, and enzymatic aspects of the fungal attack of lignin (2005) Int. Microbiol., 8, pp. 195-204 | ||
| 504 | |a Morgenstern, I., Klopman, S., Hibbett, D., Molecular evolution and diversity of lignin degrading heme peroxidases in the agaricomycetes (2008) J. Mol. Evol., 66, pp. 243-257 | ||
| 504 | |a Paice, M.G., Reid, I.D., Bourbonnais, R., Archibald, F.S., Jurasek, L., Manganese peroxidase, produced by Trametes versicolor during pulp bleaching, demethylates and delignifies kraft pulp (1993) Appl. Environ. Microbiol., 59, pp. 260-265 | ||
| 504 | |a Pérez, J., Jeffries, T.W., Roles of manganese and organic acid chelators in regulating lignin degradation and biosynthesis of peroxidases by Phanerochaete chrysosporium (1992) Appl. Environ. Microbiol., 58, pp. 2402-2409 | ||
| 504 | |a Rohene-Soustrade, I., Lung-Escarmant, B., Bono, J.J., Taris, B., Identification and partial characterization of an extracellular manganese-dependent peroxidase in Armillaria ostoyae and Armillaria mellea (1992) Eur. J. Forest Pathol., 22, pp. 227-236 | ||
| 504 | |a Ruiz-Dueñas, F.J., Camarero, S., Perez-Boada, M., Martinez, M.J., Martinez, A.T., A new versatile peroxidase from Pleurotus (2001) Biochem. Soc. Trans., 29, pp. 116-122 | ||
| 504 | |a Ruiz-Dueñas, F., Morales, M., García, E., Miki, Y., Martínez, M., Martínez, A., Substrate oxidation sites in versatile peroxidase and other basidiomycete peroxidases (2009) J. Exp. Bot., 60, pp. 441-452 | ||
| 504 | |a Ryan, D., Leukes, W., Burton, S., Improving the bioremediation of phenolic wastewaters by Trametes versicolor (2007) Bioresour. Technol., 98, pp. 579-587 | ||
| 504 | |a Salvachúa, D., Prieto, A., Martínez, A.T., Martínez, M.J., Characterization of a novel dye-decolorizing peroxidase (DyP)-type enzyme from Irpex lacteus and its application in enzymatic hydrolysis of wheat straw (2013) Appl. Environ. Microbiol., 14, pp. 4316-4324 | ||
| 504 | |a Shimokawa, T., Shoda, M., Sugano, Y., Purification and characterization of two DyP isozymes from Thanatephorus cucumeris Dec 1 specifically expressed in an air-membrane surface bioreactor (2009) J. Biosci. Bioeng., 107, pp. 113-115 | ||
| 504 | |a Sugano, Y., DyP-type peroxidases comprise a novel heme peroxidase family (2009) Cell. Mol. Life Sci., 66, pp. 1387-1403 | ||
| 504 | |a Ullrich, R., Huong, L.M., Hofrichter, M., Laccase from the medicinal mushroom Agaricus blazei: production, purification and characterization (2004) Appl. Microbiol. Biotechnol., 67, pp. 357-363 | ||
| 504 | |a Wariishi, H., Valli, K., Gold, M.H., Manganese (II) oxidation by manganese peroxidase from the basidiomycete Phanerochaete chrysosporium. Kinetic mechanism and role of chelator (1992) J. Biol. Chem., 267, pp. 23688-23695 | ||
| 504 | |a Wesenberg, D., Kyriakides, I., Agathos, S., White-rot fungi and their enzymes for the treatment of industrial dye effluents (2003) Biotechnol. Adv., 22, pp. 161-187 | ||
| 504 | |a Yaver, D.S., Xu, F., Golightly, E.J., Brown, K.M., Brown, S.H., Rey, M.W., Schneider, P., Dalbøge, H., Purification, characterization, molecular cloning, and expression of two laccase genes from the white rot basidiomycete Trametes villosa (1996) Appl. Environ. Microbiol., 62, pp. 834-841 | ||
| 504 | |a Yemendzhiev, H., Gerginova, M., Krastanov, A., Stoilova, I., Alexieva, Z., Growth of Trametes versicolor on phenol (2008) J. Ind. Microbiol. Biotechnol., 35, pp. 1309-1312 | ||
| 504 | |a Zybailov, B., Mosley, A.L., Sardiu, M.A., Coleman, M.K., Florens, L., Washburn, M.P., Statistical analysis of membrane proteome expression changes in Saccharomyces cerevisiae (2006) J. Proteome Res., 5, pp. 2339-2347 | ||
| 520 | 3 | |a The present work was carried out with the aim to analyze the secretome of Trametes versicolor BAFC 2234 grown on tomato juice medium supplemented with copper and manganese. T. versicolor BAFC 2234 was selected among diverse wood dwelling agaricomycetes from Argentina by its ability to cause a strong white rot on hardwood and in addition to show high tolerance toward phenolic compounds. A considerable number of the identified proteins were related to the degradation/modification of lignocelluloses. Hydrolases, peroxidases and phenoloxidases were the most abundant enzymes produced under the above-mentioned culture conditions. The lignin-modifying oxidoreductases laccase, manganese peroxidase (MnP) and versatile peroxidase (VP) were successfully purified - the latter for the first time from T. versicolor. The native VP protein has a molecular mass of 45. kDa and an isoelectric point of pH 3.7. The study clearly shows that complex plant-based media being rich in phenolics, such as tomato juice, can stimulate the secretion of a broad set of extracellular lignocellulolytic enzymes. Using such natural products as fungal culture media may give the opportunity to investigate plant biomass decomposition as well as the biodegradation of organic pollutants in an environment close to nature. © 2013 Elsevier B.V. |l eng | |
| 536 | |a Detalles de la financiación: German Academic Exchange Service London | ||
| 536 | |a Detalles de la financiación: Deutscher Akademischer Austauschdienst | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: European Geosciences Union, KBBE-2010-4-265397 | ||
| 536 | |a Detalles de la financiación: We gratefully acknowledge the support from the German Academic Exchange Service (DAAD) , the European Union (integrated project PEROXICATS, KBBE-2010-4-265397) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) . We thank also our co-workers M. Kluge, L. Papinutti, M. Pecyna, M. Poraj-Kobielska, T. Arnstadt, C. Liers, F. Hahn, R. Reina Prego and A. Kluttig, for scientific support, useful comments and discussions, as well as U. Schneider and M. Brandt for their technical assistance. | ||
| 593 | |a TU Dresden, International Institute Zittau, Department of Bio- and Environmental Sciences, Markt 23, 02763 Zittau, Germany | ||
| 593 | |a University of Buenos Aires, Faculty of Exact and Natural Sciences, Department of Biodiversity and Experimental Biology, Intendente Güiraldes 2160, Ciudad Universitaria, C1428EGA, Argentina | ||
| 593 | |a Interfaculty Institute for Genetics and Functional Genomics, Department of Functional Genomics, Ernst Moritz Arndt University of Greifswald, Greifswald, Germany | ||
| 690 | 1 | 0 | |a LACCASE |
| 690 | 1 | 0 | |a MANGANESE PEROXIDASE |
| 690 | 1 | 0 | |a SECRETOME |
| 690 | 1 | 0 | |a TRAMETES VERSICOLOR |
| 690 | 1 | 0 | |a VERSATILE PEROXIDASE |
| 690 | 1 | 0 | |a LACCASES |
| 690 | 1 | 0 | |a MANGANESE PEROXIDASE |
| 690 | 1 | 0 | |a SECRETOME |
| 690 | 1 | 0 | |a TRAMETES VERSICOLOR |
| 690 | 1 | 0 | |a VERSATILE PEROXIDASE |
| 690 | 1 | 0 | |a BIODEGRADATION |
| 690 | 1 | 0 | |a FRUITS |
| 690 | 1 | 0 | |a HARDWOODS |
| 690 | 1 | 0 | |a LIGNIN |
| 690 | 1 | 0 | |a PROTEINS |
| 690 | 1 | 0 | |a PURIFICATION |
| 690 | 1 | 0 | |a ENZYMES |
| 690 | 1 | 0 | |a COPPER |
| 690 | 1 | 0 | |a FUNGAL PROTEIN |
| 690 | 1 | 0 | |a HYDROLASE |
| 690 | 1 | 0 | |a LACCASE |
| 690 | 1 | 0 | |a LIGNOCELLULOSE |
| 690 | 1 | 0 | |a MANGANESE |
| 690 | 1 | 0 | |a MANGANESE PEROXIDASE |
| 690 | 1 | 0 | |a MONOPHENOL MONOOXYGENASE |
| 690 | 1 | 0 | |a PEROXIDASE |
| 690 | 1 | 0 | |a PHENOL DERIVATIVE |
| 690 | 1 | 0 | |a SECRETOME |
| 690 | 1 | 0 | |a UNCLASSIFIED DRUG |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CARBON SOURCE |
| 690 | 1 | 0 | |a CULTURE MEDIUM |
| 690 | 1 | 0 | |a DEGRADATION |
| 690 | 1 | 0 | |a ELUTION |
| 690 | 1 | 0 | |a ENZYME PURIFICATION |
| 690 | 1 | 0 | |a ENZYME SYNTHESIS |
| 690 | 1 | 0 | |a EXTRACELLULAR SPACE |
| 690 | 1 | 0 | |a FRUIT JUICE |
| 690 | 1 | 0 | |a ISOELECTRIC POINT |
| 690 | 1 | 0 | |a MOLECULAR WEIGHT |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a POLYACRYLAMIDE GEL ELECTROPHORESIS |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a PROTEIN ANALYSIS |
| 690 | 1 | 0 | |a PROTEIN SECRETION |
| 690 | 1 | 0 | |a TOMATO |
| 690 | 1 | 0 | |a TRAMETES VERSICOLOR |
| 690 | 1 | 0 | |a BIODEGRADATION |
| 690 | 1 | 0 | |a ENZYMES |
| 690 | 1 | 0 | |a FRUITS |
| 690 | 1 | 0 | |a HARDWOODS |
| 690 | 1 | 0 | |a LACCASE |
| 690 | 1 | 0 | |a LIGNINS |
| 690 | 1 | 0 | |a PROTEINS |
| 690 | 1 | 0 | |a PURIFICATION |
| 690 | 1 | 0 | |a LACCASE |
| 690 | 1 | 0 | |a MANGANESE PEROXIDASE |
| 690 | 1 | 0 | |a SECRETOME |
| 690 | 1 | 0 | |a TRAMETES VERSICOLOR |
| 690 | 1 | 0 | |a VERSATILE PEROXIDASE |
| 690 | 1 | 0 | |a CULTURE MEDIA |
| 690 | 1 | 0 | |a HYDROLASES |
| 690 | 1 | 0 | |a LYCOPERSICON ESCULENTUM |
| 690 | 1 | 0 | |a MONOPHENOL MONOOXYGENASE |
| 690 | 1 | 0 | |a OXIDOREDUCTASES |
| 690 | 1 | 0 | |a PEROXIDASE |
| 690 | 1 | 0 | |a TRAMETES |
| 651 | 4 | |a ARGENTINA | |
| 650 | 1 | 7 | |2 spines |a PH |
| 700 | 1 | |a Kellner, H. | |
| 700 | 1 | |a Levin, L. | |
| 700 | 1 | |a Jehmlich, N. | |
| 700 | 1 | |a Hofrichter, M. | |
| 700 | 1 | |a Ullrich, R. | |
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