Immunoidentification of CRF-like material in the intermaxillary glands during Bufo arenarum development

The presence of corticotropin-releasing factor like material in the intermaxillary glands was studied by immunocytochemical techniques during the metamorphosis of Bufo arenarum. The intermaxillary glands appeared at stage XV (midprometamorphosis) with CRF-like material slightly immunoreactive. These...

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Autor principal: Miranda, L.A
Otros Autores: Affanni, J.M
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Elsevier Ltd 2000
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024 7 |2 cas  |a corticotropin releasing factor, 9015-71-8, 178359-01-8, 79804-71-0, 86297-72-5, 86784-80-7 
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030 |a TICEB 
100 1 |a Miranda, L.A. 
245 1 0 |a Immunoidentification of CRF-like material in the intermaxillary glands during Bufo arenarum development 
260 |b Elsevier Ltd  |c 2000 
270 1 0 |m Miranda, L.A.; Instituto de Invest. Biotecnologicas, Instituto Tecnologico de Chascomus, Camino de Circunvalacion Laguna, Chascomus, Pcia de Buenos Aires, Argentina; email: lmiranda@intech.gov.ar 
506 |2 openaire  |e Política editorial 
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520 3 |a The presence of corticotropin-releasing factor like material in the intermaxillary glands was studied by immunocytochemical techniques during the metamorphosis of Bufo arenarum. The intermaxillary glands appeared at stage XV (midprometamorphosis) with CRF-like material slightly immunoreactive. These glands are located posterior to the premaxillae and between the nasal capsules in the roof of the mouth and are formed of alveoli or tubules. During metamorphic climax, corticotropin-releasing factor-like material was identified strongly immunostained at the apices of the secretory cells. It was observed that collecting ducts of the gland open to the anterior palatal surface suggesting that the secretion could be ingested by tadpoles. Our results clearly showed that ir-CRF-like material present in the intermaxillary glands is ingested by tadpoles during metamorphosis and could play an important role during amphibian development. (C) 2000 Harcourt Publishers Ltd.  |l eng 
536 |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas, PEI No 0344/98 
536 |a Detalles de la financiación: We wish to thank Dr Robert Denver (Michigan University, USA) for generously providing the different antisera used in this study. This work is supported by a grant from CONICET (PEI No 0344/98) 
593 |a Inst. Invest. Biotecnologicas/I., Camino de Circunvalacion Laguna, KM6(7130) Chascomús, Pcia de Buenos Aires, Argentina 
593 |a Inst. Neurociencia (INEUCI-C., Fac. de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina 
690 1 0 |a AMPHIBIA 
690 1 0 |a CORTICOTROPIN-RELEASING FACTOR 
690 1 0 |a INTERMAXILLARY GLANDS 
690 1 0 |a METAMORPHOSIS 
690 1 0 |a CORTICOTROPIN RELEASING FACTOR 
690 1 0 |a AMPHIBIA 
690 1 0 |a ANIMAL EXPERIMENT 
690 1 0 |a ANIMAL TISSUE 
690 1 0 |a ARTICLE 
690 1 0 |a DEVELOPMENTAL BIOLOGY 
690 1 0 |a FEMALE 
690 1 0 |a METAMORPHOSIS 
690 1 0 |a NONHUMAN 
690 1 0 |a PROTEIN ANALYSIS 
690 1 0 |a PROTEIN EXPRESSION 
690 1 0 |a SUBMANDIBULAR GLAND 
690 1 0 |a TISSUE DISTRIBUTION 
690 1 0 |a TOAD 
690 1 0 |a AMPHIBIA 
690 1 0 |a ANIMALIA 
690 1 0 |a ANURA 
690 1 0 |a BUFO ARENARUM 
690 1 0 |a VERTEBRATA 
700 1 |a Affanni, J.M. 
773 0 |d Elsevier Ltd, 2000  |g v. 32  |h pp. 148-152  |k n. 2  |p Tissue Cell  |x 00408166  |t Tissue and Cell 
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