Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation

In this work we characterized the infection of a primary culture of rat osteoblastic lineage cells (OBCs) with measles virus (MeV) and the effect of infection on cell differentiation and maturation. Infection of OBCs with MeV led to high titers of infectivity released early after infection. Also, an...

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Autor principal: Ayala-Peña, V.
Otros Autores: Santillán, G., Scolaro, L.
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Academic Press Inc. 2014
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Acceso en línea:Registro en Scopus
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024 7 |2 cas  |a 2 morpholino 8 phenylchromone, 154447-36-6; adenosine triphosphate, 15237-44-2, 56-65-5, 987-65-5; alkaline phosphatase, 9001-78-9; phosphatidylinositol 3 kinase, 115926-52-8; procollagen C proteinase, 68651-95-6; protein, 67254-75-5; protein kinase B, 148640-14-6; adenosine 5' o (3 thiotriphosphate), 35094-46-3 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
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100 1 |a Ayala-Peña, V. 
245 1 0 |a Experimental in vitro infection of rat osteoblasts with measles virus stimulates osteogenic differentiation 
260 |b Academic Press Inc.  |c 2014 
270 1 0 |m Scolaro, L.; Laboratorio de Virología, Departamento de Química Biológica, Universidad de Buenos Aires, Pabellón 2, Piso 4, Argentina 
506 |2 openaire  |e Política editorial 
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504 |a Urist, M.R., Bone: Formation by autoinduction (1965) Science, 150, pp. 893-899 
504 |a Ayala-Peña, V.B., Scolaro, L.A., Santillán, G.E., ATP and UTP stimulate bone morphogenetic protein-2, -4 and -5 gene expression and mineralization by rat primary osteoblasts involving PI3K/AKTpathway (2013) Exp. Cell Res., 1, pp. 2028-2036 
504 |a Csomor, P., Liktor, B., Liktor, B., Szekanecz, Z., Sziklai, I., Karosi, T., Expression of bone morphogenetic protein 2, 4, 5, and 7 correlates with histological activity of otosclerotic foci (2012) Acta Otolaryngol., 132, pp. 624-631 
504 |a Schrauwen, I., Thys, M., Vanderstraeten, K., Fransen, E., Dieltjens, N., Huyghe, J.R., Ealy, M., Van Camp, G., Association of bone morphogenetic proteins with otosclerosis (2008) J. Bone Miner. Res., 23, pp. 507-516 
504 |a Chole, R.A., McKenna, M., Pathophysiology of otosclerosis (2001) Ontol. Neurotol., 22, pp. 249-257 
504 |a Stankovic, K.M., Adachi, O., Tsuji, K., Kristiansen, A.G., Adams, J.C., Rosen, V., Differences in gene expression between the otic capsule and other bones (2010) Hear. Res., 265, pp. 83-89 
504 |a Karosi, T., Sziklai IEtiopathogenesis of otosclerosis (2010) Eur. Arch. Otorhinolaryngol., 267, pp. 1337-1349 
504 |a Schrauwen, I., Van Camp, G., The etiology of otosclerosis: A combination of genes and environment (2010) Laryngoscope, 120, pp. 1195-1202 
504 |a Niedermeyer, H.P., Gantumur, T., Neubert, W.J., Arnold, W., Measles virus and otosclerosis (2007) Adv. Otorhinolaryngol., 65, pp. 86-92 
504 |a Karosi, T., Jókay, I., Kónya, J., Petkó, M., Szabó, L.Z., Sziklai, I., Expression of measles virus receptors in otosclerotic, non-otosclerotic and in normal stapes footplates (2007) Eur. Arch. Otorhinolaryngol., 264, pp. 607-613 
504 |a Linero, F.N., Scolaro, L.A., Participation of the phosphatidylinositol 3-kinase/AKTpathway in Junín virus replication in vitro (2009) Virus Res., 145, pp. 166-170 
504 |a Gardner, C., Burke, C.W., Tesfay, M.Z., Glass, P.J., Klimstra, W.B., Ryman, K.D., Eastern and Venezuelan equine encephalitis viruses differ in their ability to infect dendritic cells and macrophages: Impact of altered cell tropism on pathogenesis (2008) J. Virol., 82, pp. 10634-10646 
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504 |a Chen, W., Foo, S.S., Rulli, N.E., Taylor, A., Sheng, K.C., Herrero, L.J., Herring, B.L., Mahalingam, S., Arthritogenic alphaviral infection perturbs osteoblast function and triggers pathologic bone loss (2014) Proc. Natl. Acad. Sci., 111, pp. 6040-6045 
504 |a Liu, W., Butts, S., Kim, H., Frenz, D.A., Negative regulation of otic capsule chondrogenesis: It can make you Smad (2007) Ann. N. Y. Acad. Sci., 1116, pp. 141-148 
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504 |a McKenna, M.J., Kristiansen, A.G., Bartley, M.L., Rogus, J.J., Haines, J.L., Association of COL1A1 and otosclerosis: Evidence for a shared genetic etiology with mild osteogenesis imperfecta (1998) Am. J. Otol., 19, pp. 604-610 
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504 |a Carsillo, M., Kim, D., Niewiesk, S., Role of AKT kinase in measles virus replication (2010) J. Virol., 84, pp. 2180-2183 
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504 |a Niewiesk, S., Schneider-Schaulies, J., Ohnimus, H., Jassoy, C., Schneider-Schaulies, S., Diamond, L., Logan, J.S., Ter Meulen, V., CD46 expression does not overcome the intracellular block of measles virus replication in transgenic rats (1997) J. Virol., 71, pp. 7969-7973 
504 |a Schlender, J., Schnorr, J., Cattomen, T., Cattaneo, R., Billeter, M.A., Ter Meulen, V., Schneider-Schaulies, S., Surface interaction of measles virus glycoproteins is necessary and sufficient for the induction of proliferative inhibition of human peripheral blood mononuclear cells (1996) Proc. Natl. Acad. Sci., 93, pp. 13194-13199 
520 3 |a In this work we characterized the infection of a primary culture of rat osteoblastic lineage cells (OBCs) with measles virus (MeV) and the effect of infection on cell differentiation and maturation. Infection of OBCs with MeV led to high titers of infectivity released early after infection. Also, analysis of mRNAs corresponding to osteogenic differentiation markers like alkaline phosphatase (ALP), bone sialo-protein (BSP) and bone morphogenetic proteins (BMPs) 1-4-5-7 in OBCs revealed higher values (2-75-fold of increment) for infected cells in comparison with uninfected controls. Differentiation of OBCs in osteogenic medium prior to infection influenced the level of stimulation induced by MeV. Furthermore, treatment of OBCs with Ly294002, a PI3K/AKT inhibitor, increased viral titers, whereas treatment with 10 μM or 100 μM ATPγS diminished MeV multiplication. In addition, increments of osteogenic differentiation markers induced by MeV infection were not modified either by treatment with Ly294002 or ATPγS. These data provide the first evidence demonstrating that MeV can infect osteoblasts in vitro leading to osteoblastic differentiation, a key feature in bone pathogenic processes like otosclerosis. © 2014 Elsevier Inc.  |l eng 
536 |a Detalles de la financiación: Universidad Nacional del Sur, 20020110100128BA 
536 |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas, PIP 112-200901-00551, PGI 24/B211, 11220090100443CO 
536 |a Detalles de la financiación: Universidad de Buenos Aires 
536 |a Detalles de la financiación: V.A.P. is a fellowship from Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET). G.S. and L.S. are members of the Research Career from CONICET (Argentina). This research was supported by Grants PIP 112-200901-00551 and 11220090100443CO from CONICET to G.S. and L.S., respectively, and PGI 24/B211 from Universidad Nacional del Sur to G.S. and 20020110100128BA from Universidad de Buenos Aires to L.S. 
593 |a Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional Del sur, San Juan 670, (B8000ICN), Bahía Blanca, Argentina 
593 |a Laboratorio de Virología, Departamento de Química Biológica, Universidad de Buenos Aires, Pabellón 2, Piso 4, Buenos Aires, 1428, Argentina 
690 1 0 |a BONE MORPHOGENETIC PROTEINS 
690 1 0 |a DIFFERENTIATION 
690 1 0 |a MEASLES VIRUS 
690 1 0 |a OSTEOBLASTS 
690 1 0 |a OTOSCLEROSIS 
690 1 0 |a 2 MORPHOLINO 8 PHENYLCHROMONE 
690 1 0 |a ADENOSINE TRIPHOSPHATE 
690 1 0 |a ALKALINE PHOSPHATASE 
690 1 0 |a BONE MORPHOGENETIC PROTEIN 4 
690 1 0 |a BONE MORPHOGENETIC PROTEIN 5 
690 1 0 |a BONE SIALO PROTEIN 
690 1 0 |a MESSENGER RNA 
690 1 0 |a OSTEOGENIC PROTEIN 1 
690 1 0 |a PHOSPHATIDYLINOSITOL 3 KINASE 
690 1 0 |a PROCOLLAGEN C PROTEINASE 
690 1 0 |a PROTEIN 
690 1 0 |a PROTEIN KINASE B 
690 1 0 |a UNCLASSIFIED DRUG 
690 1 0 |a 2-(4-MORPHOLINYL)-8-PHENYL-4H-1-BENZOPYRAN-4-ONE 
690 1 0 |a ADENOSINE 5' O (3 THIOTRIPHOSPHATE) 
690 1 0 |a ADENOSINE 5'-O-(3-THIOTRIPHOSPHATE) 
690 1 0 |a ADENOSINE TRIPHOSPHATE 
690 1 0 |a ALKALINE PHOSPHATASE 
690 1 0 |a BONE MORPHOGENETIC PROTEIN 
690 1 0 |a BONE MORPHOGENETIC PROTEIN 4 
690 1 0 |a CHROMONE DERIVATIVE 
690 1 0 |a DRUG DERIVATIVE 
690 1 0 |a MORPHOLINE DERIVATIVE 
690 1 0 |a PHOSPHATIDYLINOSITOL 3 KINASE 
690 1 0 |a ANIMAL CELL 
690 1 0 |a ANIMAL EXPERIMENT 
690 1 0 |a ANIMAL MODEL 
690 1 0 |a ARTICLE 
690 1 0 |a BONE DEVELOPMENT 
690 1 0 |a CELL DIFFERENTIATION 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a EXPERIMENTAL INFECTION 
690 1 0 |a IN VITRO STUDY 
690 1 0 |a MEASLES 
690 1 0 |a MEASLES VIRUS 
690 1 0 |a NONHUMAN 
690 1 0 |a OSTEOBLAST 
690 1 0 |a PROTEIN EXPRESSION 
690 1 0 |a RAT 
690 1 0 |a STIMULATION 
690 1 0 |a VIRUS LOAD 
690 1 0 |a ANIMAL 
690 1 0 |a ARTICLE 
690 1 0 |a BIOSYNTHESIS 
690 1 0 |a BONE 
690 1 0 |a CELL CULTURE 
690 1 0 |a DIFFERENTIATION 
690 1 0 |a DRUG ANTAGONISM 
690 1 0 |a DRUG EFFECT 
690 1 0 |a METABOLISM 
690 1 0 |a OTOSCLEROSIS 
690 1 0 |a PHYSIOLOGY 
690 1 0 |a VIROLOGY 
690 1 0 |a VIRUS REPLICATION 
690 1 0 |a BONE MORPHOGENETIC PROTEINS 
690 1 0 |a DIFFERENTIATION 
690 1 0 |a MEASLES VIRUS 
690 1 0 |a OSTEOBLASTS 
690 1 0 |a OTOSCLEROSIS 
690 1 0 |a ADENOSINE TRIPHOSPHATE 
690 1 0 |a ALKALINE PHOSPHATASE 
690 1 0 |a ANIMALS 
690 1 0 |a BONE AND BONES 
690 1 0 |a BONE MORPHOGENETIC PROTEIN 4 
690 1 0 |a CELL DIFFERENTIATION 
690 1 0 |a CELLS, CULTURED 
690 1 0 |a CHROMONES 
690 1 0 |a MEASLES VIRUS 
690 1 0 |a MORPHOLINES 
690 1 0 |a OSTEOBLASTS 
690 1 0 |a OTOSCLEROSIS 
690 1 0 |a PHOSPHATIDYLINOSITOL 3-KINASES 
690 1 0 |a RATS 
690 1 0 |a VIRUS REPLICATION 
650 1 7 |2 spines  |a OSTEOGENESIS 
700 1 |a Santillán, G. 
700 1 |a Scolaro, L. 
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