Modulating surface density of proteins via caged surfaces and controlled light exposure

Mostrar otras versiones (1)

We demonstrate the possibility of tuning the degree of functionalization of a surface using photoactivatable chemistries and controlled light exposure. A photosensitive organosilane with a protected amine terminal group and a tetraethyleneglycol spacer was synthesized. A o-nitrobenzyl cage was used...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autor principal: Álvarez, M.
Otros Autores: Alonso, J.M, Filevich, O., Bhagawati, M., Etchenique, R., Piehler, J., Del Campo, A.
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2011
Acceso en línea:Registro en Scopus
DOI
Handle
Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central Dr. Luis F. Leloir (FCEN) de Universidad de Buenos Aires
LEADER 10528caa a22015137a 4500
001 PAPER-23707
003 AR-BaUEN
005 20230518205526.0
008 190411s2011 xx ||||fo|||| 00| 0 eng|d
024 7 |2 scopus  |a 2-s2.0-79952582954 
024 7 |2 cas  |a protein, 67254-75-5; Photosensitizing Agents; Proteins 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a LANGD 
100 1 |a Álvarez, M. 
245 1 0 |a Modulating surface density of proteins via caged surfaces and controlled light exposure 
260 |c 2011 
270 1 0 |m Del Campo, A.; Max-Planck-Institut für Polymerforschung, Ackermannweg 10, 55128 Mainz, Germany; email: delcampo@mpip-mainz.mpg.de 
506 |2 openaire  |e Política editorial 
504 |a Yuan, W.F., Jiang, G.Y., Wang, J.X., Wang, G.J., Song, Y.L., Jiang, L., (2006) Macromolecules, 39, p. 1300 
504 |a Auernheimer, J., Dahmen, C., Hersel, U., Bausch, A., Kessler, H., (2005) J. Am. Chem. Soc., 127, p. 16107 
504 |a Dattilo, D., Armelao, L., Fois, G., Mistura, G., Maggini, M., (2007) Langmuir, 23, p. 12945 
504 |a Dulcey, C.S., Georger, J.H., Krauthamer, V., Stenger, D.A., Fare, T.L., Calvert, J.M., (1991) Science, 252, p. 551 
504 |a Tarlov, M.J., Burgess, D.R.F., Gillen, G., (1993) J. Am. Chem. Soc., 115, p. 5305 
504 |a Westcott, N.P., Yousaf, M.N., (2008) Langmuir, 24, p. 2261 
504 |a Brandow, S.L., Chen, S., Aggarwal, R., Dulcey, C.S., Calvert, J.M., Dressick, W.J., (1999) Langmuir, 15, p. 5429 
504 |a Monsathaporn, S., Effenberger, F., (2004) Langmuir, 20, p. 10375 
504 |a Ito, Y., (2006) Biotechnol. Prog., 22, p. 924 
504 |a Kado, Y., Mitsuishi, M., Miyashita, T., (2005) Adv. Mater., 17, p. 1857 
504 |a Kanoh, N., Kumashiro, S., Simizu, S., Kondoh, Y., Hatakeyama, S., Tashiro, H., Osada, H., (2003) Angew Chem Int Edit, 42, p. 5584 
504 |a White, M.A., Johnson, J.A., Koberstein, J.T., Turro, N.J., (2006) J. Am. Chem. Soc., 128, p. 11356 
504 |a Alonso, J.M., Reichel, A., Piehler, J., Del Campo, A., (2008) Langmuir, 24, p. 448 
504 |a Del Campo, A., Boos, D., Spiess, H.W., Jonas, U., (2005) Angew Chem Int Edit, 44, p. 4707 
504 |a Fodor, S.P.A., Read, J.L., Pirrung, M.C., Stryer, L., Lu, A.T., Solas, D., (1991) Science, 251, p. 767 
504 |a Petersen, S., Alonso, J.M., Specht, A., Duodu, P., Goeldner, M., Del Campo, A., (2008) Angew. Chem., Int. Ed., 47, p. 3192 
504 |a Pirrung, M.C., Fallon, L., McGall, G., (1998) J. Org. Chem., 63, p. 241 
504 |a Pirrung, M.C., Huang, C.Y., (1996) Bioconjugate Chem, 7, p. 317 
504 |a Stegmaier, P., Alonso, J.M., Del Campo, A., (2008) Langmuir, 24, p. 11872 
504 |a Stegmaier, P., Del Campo, A., (2009) Chemphyschem, 10, p. 357 
504 |a Bochet, C.G., (2002) J. Chem. Soc.-Perkin Trans. 1, p. 125 
504 |a Pillai, V.N.R., (1980) Synthesis, p. 1 
504 |a Mayer, G., Heckel, A., (2006) Angew Chem Int Edit, 45, p. 4900 
504 |a Sundberg, S.A., Barrett, R.W., Pirrung, M., Lu, A.L., Kiangsoontra, B., Holmes, C.P., (1995) J. Am. Chem. Soc., 117, p. 12050 
504 |a Fodor, S.P.A., Rava, R.P., Huang, X.H.C., Pease, A.C., Holmes, C.P., Adams, C.L., (1993) Nature, 364, p. 555 
504 |a Pirrung, M.C., (2002) Angew Chem Int Edit, 41, p. 1277 
504 |a Pellois, J.P., Wang, W., Gao, X.L., (2000) J Comb Chem, 2, p. 355 
504 |a McGall, G., Labadie, J., Brock, P., Wallraff, G., Nguyen, T., Hinsberg, W., (1996) P Natl Acad Sci USA, 93, p. 13555 
504 |a Pease, A.C., Solas, D., Sullivan, E.J., Cronin, M.T., Holmes, C.P., Fodor, S.P.A., (1994) P Natl Acad Sci USA, 91, p. 5022 
504 |a Gao, X.L., Yu, P.L., Leproust, E., Sonigo, L., Pellois, J.P., Zhang, H., (1998) J. Am. Chem. Soc., 120, p. 12698 
504 |a Singh-Gasson, S., Green, R.D., Yue, Y.J., Nelson, C., Blattner, F., Sussman, M.R., Cerrina, F., (1999) Nat. Biotechnol., 17, p. 974 
504 |a McGall, G.H., Barone, A.D., Diggelmann, M., Fodor, S.P.A., Gentalen, E., Ngo, N., (1997) J. Am. Chem. Soc., 119, p. 5081 
504 |a Li, S.W., Bowerman, D., Marthandan, N., Klyza, S., Luebke, K.J., Garner, H.R., Kodadek, T., (2004) J. Am. Chem. Soc., 126, p. 4088 
504 |a Vossmeyer, T., Deionno, E., Heath, J.R., (1997) Angew. Chem., Int. Ed., 36, p. 1080 
504 |a Vossmeyer, T., Jia, S., Deionno, E., Diehl, M.R., Kim, S.H., Peng, X., Alivisatos, A.P., Heath, J.R., (1998) J. Appl. Phys., 84, p. 3664 
504 |a Jonas, U., Del Campo, A., Kruger, C., Glasser, G., Boos, D., (2002) P Natl Acad Sci USA, 99, p. 5034 
504 |a Millaruelo, M., Eng, L.M., Merting, M., Pilch, B., Oertel, U., Opitz, J., Sieczkowska, B., Voit, B., (2006) Langmuir, 22, p. 9446 
504 |a Lee, K.N., Shin, D.S., Lee, Y.S., Kim, Y.K., (2003) Journal of Micromechanics and Microengineering, 13, p. 18 
504 |a Bhagawati, M., Lata, S., Tampe, R., Piehler, J., (2010) J. Am. Chem. Soc., 132, p. 5932 
504 |a Grunwald, C., Schulze, K., Reichel, A., Weiss, V.U., Blaas, D., Piehler, J., Wiesmuller, K.H., Tampe, R., (2010) P Natl Acad Sci USA, 107, p. 6146 
504 |a Dillmore, W.S., Yousaf, M.N., Mrksich, M., (2004) Langmuir, 20, p. 7223 
504 |a Ryan, D., Parviz, B.A., Linder, V., Semetey, V., Sia, S.K., Su, J., Mrksich, M., Whitesides, G.M., (2004) Langmuir, 20, p. 9080 
504 |a Hower, J.C., He, Y., Bernards, M.T., Jiang, S.Y., (2006) J. Chem. Phys., 125, p. 214704 
504 |a Morra, M., (2000) J Biomat Sci-Polym e, 11, p. 547 
504 |a Alonso, J.M., Reichel, A., Piehler, J., Del Campo, A., (2008) Langmuir, 24, p. 448 
504 |a Lata, S., Piehler, J., (2005) Anal. Chem., 77, p. 1096 
504 |a Bramblett, A.L., Boeckl, M.S., Hauch, K.D., Ratner, B.D., Sasaki, T., Rogers, J.W., (2002) Surf. Interface Anal., 33, p. 506 
504 |a Caruso, F., Kurth, D.G., Volkmer, D., Koop, M.J., Muller, A., (1998) Langmuir, 14, p. 3462 
504 |a Brecht, A., Gauglitz, G., (1995) Biosens. Bioelectron., 10, p. 923 
504 |a Hanel, C., Gauglitz, G., (2002) Anal. Bioanal. Chem., 372, p. 91 
504 |a Schmitt, H.M., Brecht, A., Piehler, J., Gauglitz, G., (1997) Biosens. Bioelectron., 12, p. 809 
504 |a Love, J.C., Estroff, L.A., Kriebel, J.K., Nuzzo, R.G., Whitesides, G.M., (2005) Chem. Rev., 105, p. 1103 
504 |a Dubois, L.H., Nuzzo, R.G., (1992) Annu. Rev. Phys. Chem., 43, p. 437 
504 |a Bochet, C.G., (2001) Angew Chem Int Edit, 40, p. 2071 
504 |a Piehler, J., Brecht, A., Gauglitz, G., (1996) Anal. Chem., 68, p. 139 
504 |a Reichel, A., Schaible, D., Al Furoukh, N., Cohen, M., Schreiber, G., Piehler, J., (2007) Anal. Chem., 79, p. 8590 
520 3 |a We demonstrate the possibility of tuning the degree of functionalization of a surface using photoactivatable chemistries and controlled light exposure. A photosensitive organosilane with a protected amine terminal group and a tetraethyleneglycol spacer was synthesized. A o-nitrobenzyl cage was used as the photoremovable group to cage the amine functionality. Surfaces with phototunable amine densities were generated by controlled irradiation of silica substrates modified with the photosensitive anchor. Protein layers with different densities could be obtained by successive coupling and assembly steps. Protein surface concentrations were quantified by reflectance interference. Our results demonstrate that the protein density correlates with the photogenerated ligand density. The density control was proved over four coupling steps (biotin, SAv, BTtris-NTA, MBP, or GFP), indicating that the interactions between underlying layer and soluble targets are highly specific and the immobilized targets at the four levels maintain their full functionality. Protein micropatterns with a gradient of protein density were also obtained. © 2011 American Chemical Society.  |l eng 
593 |a Max-Planck-Institut für Polymerforschung, Ackermannweg 10, 55128 Mainz, Germany 
593 |a Max Planck Institut für Metallforschung, Heisenbergstrasse 3, 70569 Stuttgart, Germany 
593 |a Departamento de Quimica Inorganica, Analitica y Quimica Fisica, INQUIMAE, Universidad de Buenos Aires, Ciudad Universitaria Pabellón 2 Piso 3, C1428EHA, Buenos Aires, Argentina 
593 |a Universität Osnabrück, Barbarastrasse 11, 49076 Osnabrück, Germany 
593 |a CIC NanoGUNE Consolider, Tolosa Hiribidea, 76, 20018 San Sebastián, Spain 
690 1 0 |a AMINE FUNCTIONALITY 
690 1 0 |a DEGREE OF FUNCTIONALIZATION 
690 1 0 |a DENSITY CONTROL 
690 1 0 |a LIGAND DENSITY 
690 1 0 |a LIGHT EXPOSURE 
690 1 0 |a ORGANOSILANES 
690 1 0 |a PHOTO-TUNABLE 
690 1 0 |a PHOTOACTIVATABLE 
690 1 0 |a PROTEIN DENSITY 
690 1 0 |a PROTEIN LAYERS 
690 1 0 |a PROTEIN MICROPATTERNS 
690 1 0 |a PROTEIN SURFACE 
690 1 0 |a SILICA SUBSTRATE 
690 1 0 |a SURFACE DENSITY 
690 1 0 |a TERMINAL GROUPS 
690 1 0 |a UNDERLYING LAYERS 
690 1 0 |a LIGHT SENSITIVE MATERIALS 
690 1 0 |a ORGANIC COMPOUNDS 
690 1 0 |a PHOTOSENSITIVITY 
690 1 0 |a SILICA 
690 1 0 |a PROTEINS 
690 1 0 |a PHOTOSENSITIZING AGENT 
690 1 0 |a PROTEIN 
690 1 0 |a ARTICLE 
690 1 0 |a CHEMISTRY 
690 1 0 |a LIGHT 
690 1 0 |a PHOTOCHEMISTRY 
690 1 0 |a SURFACE PROPERTY 
690 1 0 |a SYNTHESIS 
690 1 0 |a LIGHT 
690 1 0 |a PHOTOCHEMICAL PROCESSES 
690 1 0 |a PHOTOSENSITIZING AGENTS 
690 1 0 |a PROTEINS 
690 1 0 |a SURFACE PROPERTIES 
700 1 |a Alonso, J.M. 
700 1 |a Filevich, O. 
700 1 |a Bhagawati, M. 
700 1 |a Etchenique, R. 
700 1 |a Piehler, J. 
700 1 |a Del Campo, A. 
773 0 |d 2011  |g v. 27  |h pp. 2789-2795  |k n. 6  |p Langmuir  |x 07437463  |t Langmuir 
856 4 1 |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-79952582954&doi=10.1021%2fla104511x&partnerID=40&md5=0a7266098f2153f5ea18634ccbf01d54  |y Registro en Scopus 
856 4 0 |u https://doi.org/10.1021/la104511x  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_07437463_v27_n6_p2789_Alvarez  |y Handle 
856 4 0 |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07437463_v27_n6_p2789_Alvarez  |y Registro en la Biblioteca Digital 
961 |a paper_07437463_v27_n6_p2789_Alvarez  |b paper  |c PE 
962 |a info:eu-repo/semantics/article  |a info:ar-repo/semantics/artículo  |b info:eu-repo/semantics/publishedVersion 
963 |a VARI 
999 |c 84660 

Ejemplares similares