Cadmium modulates NADPH oxidase activity and expression in sunflower leaves

The production of reactive oxygen species (ROS) and the ways by which ROS are generated are very important facts related to heavy metal toxicity in plants. In this work, superoxide anion (O2·-) generation diminished in cadmium treated sunflower (Helianthus annuus L.) leaf discs, and this reduction w...

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Autor principal: Groppa, M.D
Otros Autores: Ianuzzo, M.P, Rosales, E.P, Vázquez, S.C, Benavides, M.P
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Kluwer Academic Publishers 2012
Acceso en línea:Registro en Scopus
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100 1 |a Groppa, M.D. 
245 1 0 |a Cadmium modulates NADPH oxidase activity and expression in sunflower leaves 
260 |b Kluwer Academic Publishers  |c 2012 
270 1 0 |m Groppa, M. D.; Cátedra de Química Biológica Vegetal, Departamento de Química Biológica, Universidad de Buenos Aires, Junín 956, 1113 Buenos Aires, Argentina; email: mgroppa@ffyb.uba.ar 
506 |2 openaire  |e Política editorial 
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520 3 |a The production of reactive oxygen species (ROS) and the ways by which ROS are generated are very important facts related to heavy metal toxicity in plants. In this work, superoxide anion (O2·-) generation diminished in cadmium treated sunflower (Helianthus annuus L.) leaf discs, and this reduction was time and Cd-concentration dependent. In line with these findings, we observed that NADPH-dependent oxidase activity was significantly inhibited by 0. 1 and 0. 5 mM Cd2+ treatments and the expression of the NADPH oxidase putative gene related to O2·- synthesis in sunflower leaves was 83 % inhibited by 0. 1 mM CdCl2 and almost completely depleted by 0. 5 mM CdCl2. © 2012 Springer Science+Business Media B.V.  |l eng 
536 |a Detalles de la financiación: Universidad de Buenos Aires, B017 
536 |a Detalles de la financiación: Received 28 December 2009, accepted 25 January 2011. Abbreviations: DPI - diphenyl iodonium; NBT - nitroblue tetrazolium; PMSF - phenylmethylsulphonylfluoride; ROS - reactive oxygen species; SOD - superoxide dismutase. Acknowledgements: This work was supported by the University of Buenos Aires (Project B017). M.D. Groppa, S. Vázquez and M.P. Benavides are also researchers of the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). * Corresponding author; fax: (+54) 11 4508-3645, e-mail: mgroppa@ffyb.uba.ar 
593 |a Cátedra de Química Biológica Vegetal, Departamento de Química Biológica, Universidad de Buenos Aires, Junín 956, 1113 Buenos Aires, Argentina 
593 |a Cátedra de Microbiología Industrial y Biotecnología, Departamento de Microbiología, Inmunología y Biotecnologia Facultad de Farmacia y Bioquimica Universidad de Buenos Aires, Junín 956, 1113 Buenos Aires, Argentina 
690 1 0 |a HELIANTHUS ANNUUS 
690 1 0 |a REACTIVE OXYGEN SPECIES 
690 1 0 |a SUPEROXIDE ANION 
690 1 0 |a HELIANTHUS 
690 1 0 |a HELIANTHUS ANNUUS 
700 1 |a Ianuzzo, M.P. 
700 1 |a Rosales, E.P. 
700 1 |a Vázquez, S.C. 
700 1 |a Benavides, M.P. 
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