Progesterone effects on neuronal ultrastructure and expression of microtubule-associated protein 2 (MAP2) in bats with acute spinal cord injury

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(1) Following acute spinal cord injury, progesterone modulates several molecules essential for motoneuron function, although the morphological substrates for these effects are unknown. (2) The present study analyzed morphological changes in motoneurons distal to the lesion site from rats with or wit...

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Autor principal: González, S.L
Otros Autores: López-Costa, J.J, Labombarda, F., Deniselle, M.C.G, Guennoun, R., Schumacher, M., De Nicola, A.F
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2009
Materias:
proluton, Schering, Argentina
Acceso en línea:Registro en Scopus
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Biblioteca Central Dr. Luis F. Leloir (FCEN) de Universidad de Buenos Aires
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024 7 |2 cas  |a progesterone, 57-83-0; Microtubule-Associated Proteins; Mtap2 protein, rat; Progesterone, 57-83-0 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a CMNED 
100 1 |a González, S.L. 
245 1 0 |a Progesterone effects on neuronal ultrastructure and expression of microtubule-associated protein 2 (MAP2) in bats with acute spinal cord injury 
260 |c 2009 
270 1 0 |m De Nicola, A. F.; Instituto de Biologia y Medicina Experimental, Obligado 2490, Buenos Aires 1428, Argentina; email: denicola@dna.uba.ar 
506 |2 openaire  |e Política editorial 
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520 3 |a (1) Following acute spinal cord injury, progesterone modulates several molecules essential for motoneuron function, although the morphological substrates for these effects are unknown. (2) The present study analyzed morphological changes in motoneurons distal to the lesion site from rats with or without progesterone treatment. We employed electron microscopy to study changes in nucleus and cytoplasm and immunohistochemistry for the microtubule-associated protein 2 (MAP2) for changes in cytoskeleton. (3) After spinal cord injury, the nucleoplasm appeared more finely dispersed resulting in reduced electron opacity and the nucleus adopted an eccentric position. Changes of perikarya included dissolution of Nissl bodies and dissociation of polyribosomes (chromatolysis). After progesterone treatment for 3 days, the deafferented motoneurons now presented a clumped nucleoplasm, a better-preserved rough endoplasmic reticulum and absence of chromatolysis. Progesterone partially prevented development of nuclear eccentricity. Whereas 50% of injured motoneurons showed nuclear eccentricity, only 16% presented this phenotype after receiving progesterone. Additionally, injured rats showed reduced immunostaining for MAP2 in dendrites, pointing to cytoskeleton abnormalities, whereas progesterone treatment attenuated the injury-induced loss of MAP2. (4) Our data indicated that progesterone maintained in part neuronal ultrastructure, attenuated chromatolysis, and preclude the loss of MAP2, suggesting a protective effect during the early phases of spinal cord injury. © 2008 Springer Science+Business Media, LLC.  |l eng 
536 |a Detalles de la financiación: Universidad de Buenos Aires, M808, M022 
536 |a Detalles de la financiación: Inter-American Development Bank, PICT 2004 # 25610 
536 |a Detalles de la financiación: National Council for Scientific Research 
536 |a Detalles de la financiación: Fondo para la Investigación Científica y Tecnológica 
536 |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas, PIP 5542 
536 |a Detalles de la financiación: Acknowledgments The authors acknowledge the support of FONCYT (BID OC AR PICT 2004 # 25610), the National Research Council of Argentina (CONICET, PIP 5542), and the University of Buenos Aires (M022 and M808). We deeply thank Ms. Mariana López Ravasio for electron microscopic techniques and Juan Pablo Corazza for help with photography. 
593 |a Laboratory of Neuroendocrine Biochemistry, Instituto de Biología y Medicina Experimental, Obligado 2490, Buenos Aires 1428, Argentina 
593 |a Department of Human Biochemistry, Faculty of Medicine, University of Buenos Aires, Buenos Aires, Argentina 
593 |a Instituto de Biología Celular y Neurociencias Prof. Eduardo de Robertis, Faculty of Medicine, University of Buenos Aires, Buenos Aires, Argentina 
593 |a UMR 788 INSERM, University Paris 11: (Steroids Neuroprotection and Neuroregeneration), Kremlin-Bicêtre 94276, France 
593 |a Instituto de Biologia y Medicina Experimental, Obligado 2490, Buenos Aires 1428, Argentina 
690 1 0 |a CHROMATOLYSIS 
690 1 0 |a ELECTRON MICROSCOPY 
690 1 0 |a MAP2 
690 1 0 |a MOTONEURON 
690 1 0 |a NEUROPROTECTION 
690 1 0 |a PROGESTERONE 
690 1 0 |a SPINAL CORD INJURY 
690 1 0 |a MICROTUBULE ASSOCIATED PROTEIN 2 
690 1 0 |a MICROTUBULE PROTEIN 
690 1 0 |a PROGESTERONE 
690 1 0 |a ANIMAL CELL 
690 1 0 |a ANIMAL EXPERIMENT 
690 1 0 |a ANIMAL MODEL 
690 1 0 |a ARTICLE 
690 1 0 |a CELL ULTRASTRUCTURE 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a CYTOPLASM 
690 1 0 |a CYTOSKELETON 
690 1 0 |a DENDRITIC SPINE 
690 1 0 |a ELECTRON MICROSCOPY 
690 1 0 |a IMMUNOHISTOCHEMISTRY 
690 1 0 |a MALE 
690 1 0 |a MOTONEURON 
690 1 0 |a NEUROMODULATION 
690 1 0 |a NEUROPROTECTION 
690 1 0 |a NISSL SUBSTANCE 
690 1 0 |a NONHUMAN 
690 1 0 |a NUCLEUS ACCUMBENS 
690 1 0 |a PERIKARYON 
690 1 0 |a POLYSOME 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a RAT 
690 1 0 |a ROUGH ENDOPLASMIC RETICULUM 
690 1 0 |a SPINAL CORD INJURY 
690 1 0 |a THERAPY EFFECT 
690 1 0 |a TREATMENT DURATION 
690 1 0 |a TREATMENT OUTCOME 
690 1 0 |a TREATMENT RESPONSE 
690 1 0 |a ACUTE DISEASE 
690 1 0 |a ANIMALS 
690 1 0 |a CELL NUCLEOLUS 
690 1 0 |a IMMUNOHISTOCHEMISTRY 
690 1 0 |a MALE 
690 1 0 |a MICROTUBULE-ASSOCIATED PROTEINS 
690 1 0 |a MOTOR NEURONS 
690 1 0 |a NEURONS 
690 1 0 |a PROGESTERONE 
690 1 0 |a RATS 
690 1 0 |a RATS, SPRAGUE-DAWLEY 
690 1 0 |a SPINAL CORD INJURIES 
690 1 0 |a RATTUS 
653 0 0 |a proluton, Schering, Argentina 
700 1 |a López-Costa, J.J. 
700 1 |a Labombarda, F. 
700 1 |a Deniselle, M.C.G. 
700 1 |a Guennoun, R. 
700 1 |a Schumacher, M. 
700 1 |a De Nicola, A.F. 
773 0 |d 2009  |g v. 29  |h pp. 27-39  |k n. 1  |p Cell. Mol. Neurobiol.  |x 02724340  |t Cellular and Molecular Neurobiology 
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856 4 0 |u https://doi.org/10.1007/s10571-008-9291-0  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_02724340_v29_n1_p27_Gonzalez  |y Handle 
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999 |c 84186 

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