Mixed nicotinic-muscarinic properties of the α9 nicotinic cholinergic receptor
The rat α9 nicotinic acetylcholine receptor (nAChR) was expressed in Xenopus laevis oocytes and tested for its sensitivity to a wide variety of cholinergic compounds. Acetylcholine (ACh), carbachol, choline and methylcarbachol elicited agonist-evoked currents, giving maximal or near maximal response...
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Elsevier Ltd
2000
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| LEADER | 19308caa a22016697a 4500 | ||
|---|---|---|---|
| 001 | PAPER-2244 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203138.0 | ||
| 008 | 190411s2000 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0033814975 | |
| 024 | 7 | |2 cas |a acetylcholine, 51-84-3, 60-31-1, 66-23-9; atropine, 51-55-8, 55-48-1; bethanechol, 590-63-6, 674-38-4, 91609-06-2; carbachol, 462-58-8, 51-83-2; choline, 123-41-1, 13232-47-8, 1927-06-6, 4858-96-2, 62-49-7, 67-48-1; cytisine, 485-35-8; dicholine suberate, 3810-71-7, 7262-79-5; dihydro beta erythroidine, 23255-54-1; epibatidine, 140111-52-0, 148152-66-3, 152378-30-8; gallamine, 153-76-4; mecamylamine, 60-40-2, 826-39-1; methylcarbachol, 14721-69-8; methyllycaconitine, 21019-30-7, 72629-98-2; muscarine, 300-54-9; nicotine, 54-11-5; phenyltrimethylammonium, 138-24-9, 3426-74-2; pilocarpine, 148-72-1, 54-71-7, 92-13-7; pirenzepine, 28797-61-7, 29868-97-1; tetramethylammonium, 51-92-3; [4 [(3 chlorophenyl)carbamoyloxy] 2 butynyl]trimethylammonium, 55-45-8 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a NEPHB | ||
| 100 | 1 | |a Verbitsky, M. | |
| 245 | 1 | 0 | |a Mixed nicotinic-muscarinic properties of the α9 nicotinic cholinergic receptor |
| 260 | |b Elsevier Ltd |c 2000 | ||
| 270 | 1 | 0 | |m Belen Elgoyhen, A.; Istituto de Investigaciones, Consejo Nac. Invest. Cientificas, Universidad de Buenos Aires, Vuelta de Obligado 2490, Buenos Aires 1428, Argentina; email: elgoyhen@dna.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 520 | 3 | |a The rat α9 nicotinic acetylcholine receptor (nAChR) was expressed in Xenopus laevis oocytes and tested for its sensitivity to a wide variety of cholinergic compounds. Acetylcholine (ACh), carbachol, choline and methylcarbachol elicited agonist-evoked currents, giving maximal or near maximal responses. Both the nicotinic agonist suberyldicholine as well as the muscarinic agonists McN-A-343 and methylfurtrethonium behaved as weak partial agonists of the receptor. Most classical cholinergic compounds tested, being either nicotinic (nicotine, epibatidine, cytisine, methyllycaconitine, mecamylamine, dihydro-β-erythroidine), or muscarinic (muscarine, atropine, gallamine, pilocarpine, bethanechol) agonists and antagonists, blocked the recombinant α9 receptor. Block by nicotine, epibatidine, cytisine, methyllycaconitine and atropine was overcome at high ACh concentrations, suggesting a competitive type of block. The present results indicate that α9 displays mixed nicotinic-muscarinic features that resemble the ones described for the cholinergic receptor of cochlear outer hair cells (OHCs). We suggest that α9 contains the structural determinants responsible for the pharmacological properties of the native receptor. Copyright (C) 2000 Elsevier Science Ltd. |l eng | |
| 536 | |a Detalles de la financiación: Agencia Nacional de Promoción Científica y Tecnológica | ||
| 536 | |a Detalles de la financiación: Howard Hughes Medical Institute | ||
| 536 | |a Detalles de la financiación: Fundación Antorchas | ||
| 536 | |a Detalles de la financiación: National Organization for Hearing Research Foundation | ||
| 536 | |a Detalles de la financiación: This work was supported by an International Research Scholar grant from the Howard Hughes Medical Institute, the National Organization for Hearing Research (USA), Fundación Antorchas and Agencia Nacional de Promoción Cientı́fica y Tecnológica (Argentina). | ||
| 593 | |a Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad de Buenos Aires, Vuelta de Obligado 2490, Buenos Aires 1428, Argentina | ||
| 593 | |a Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires 1428, Argentina | ||
| 690 | 1 | 0 | |a COCHLEA |
| 690 | 1 | 0 | |a HAIR CELLS |
| 690 | 1 | 0 | |a ION CHANNELS |
| 690 | 1 | 0 | |a MUSCARINIC RECEPTORS |
| 690 | 1 | 0 | |a NEUROTRANSMITTER RECEPTORS |
| 690 | 1 | 0 | |a NICOTINIC RECEPTORS |
| 690 | 1 | 0 | |a ACETYLCHOLINE |
| 690 | 1 | 0 | |a ATROPINE |
| 690 | 1 | 0 | |a BETHANECHOL |
| 690 | 1 | 0 | |a CARBACHOL |
| 690 | 1 | 0 | |a CHOLINE |
| 690 | 1 | 0 | |a CHOLINERGIC RECEPTOR STIMULATING AGENT |
| 690 | 1 | 0 | |a CYTISINE |
| 690 | 1 | 0 | |a DICHOLINE SUBERATE |
| 690 | 1 | 0 | |a DIHYDRO BETA ERYTHROIDINE |
| 690 | 1 | 0 | |a EPIBATIDINE |
| 690 | 1 | 0 | |a GALLAMINE |
| 690 | 1 | 0 | |a ION CHANNEL |
| 690 | 1 | 0 | |a MECAMYLAMINE |
| 690 | 1 | 0 | |a METHYLCARBACHOL |
| 690 | 1 | 0 | |a METHYLLYCACONITINE |
| 690 | 1 | 0 | |a MUSCARINE |
| 690 | 1 | 0 | |a MUSCARINIC AGENT |
| 690 | 1 | 0 | |a MUSCARINIC RECEPTOR |
| 690 | 1 | 0 | |a MUSCARINIC RECEPTOR BLOCKING AGENT |
| 690 | 1 | 0 | |a NEUROTRANSMITTER RECEPTOR |
| 690 | 1 | 0 | |a NICOTINE |
| 690 | 1 | 0 | |a NICOTINIC AGENT |
| 690 | 1 | 0 | |a NICOTINIC RECEPTOR |
| 690 | 1 | 0 | |a NICOTINIC RECEPTOR BLOCKING AGENT |
| 690 | 1 | 0 | |a PHENYLTRIMETHYLAMMONIUM |
| 690 | 1 | 0 | |a PILOCARPINE |
| 690 | 1 | 0 | |a PIRENZEPINE |
| 690 | 1 | 0 | |a TETRAMETHYLAMMONIUM |
| 690 | 1 | 0 | |a [4 [(3 CHLOROPHENYL)CARBAMOYLOXY] 2 BUTYNYL]TRIMETHYLAMMONIUM |
| 690 | 1 | 0 | |a ANIMAL CELL |
| 690 | 1 | 0 | |a BINDING SITE |
| 690 | 1 | 0 | |a COCHLEA |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a DRUG RECEPTOR BINDING |
| 690 | 1 | 0 | |a ELECTROPHYSIOLOGY |
| 690 | 1 | 0 | |a HAIR CELL |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a OOCYTE |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a REVIEW |
| 690 | 1 | 0 | |a XENOPUS LAEVIS |
| 653 | 0 | 0 | |a mcn-a-343, RBI, United States |
| 700 | 1 | |a Rothlin, C.V. | |
| 700 | 1 | |a Katz, E. | |
| 700 | 1 | |a Belén Elgoyhen, A. | |
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