Electrochemical behavior of polyphenol oxidase immobilized in self-assembled structures layer by layer with cationic polyallylamine
We report here a novel bioelectrode based on self-assembled multilayers of polyphenol oxidase intercalated with cationic polyallylamine built up on a thiol-modified gold surface. We use an immobilization strategy previously described by Hodak J. et al. (Langmuir 1997, 13, 2708-2716) Quartz crystal m...
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2000
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| LEADER | 07070caa a22008657a 4500 | ||
|---|---|---|---|
| 001 | PAPER-2239 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203138.0 | ||
| 008 | 190411s2000 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0034332643 | |
| 024 | 7 | |2 cas |a Catechol Oxidase, 1.10.3.1; Enzymes, Immobilized; Indicators and Reagents; Polyamines; polyallylamine, 30551-89-4 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a ANCHA | ||
| 100 | 1 | |a Forzani, E.S. | |
| 245 | 1 | 0 | |a Electrochemical behavior of polyphenol oxidase immobilized in self-assembled structures layer by layer with cationic polyallylamine |
| 260 | |c 2000 | ||
| 270 | 1 | 0 | |m Solis, V.M.; Departamento de Fisicoquimica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Ciudad Universitaria, 5000 Cordoba, Argentina; email: vsolis@fisquim.fcq.unc.edu.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Lvov, Y., Essler, F., Decher, G., (1993) J. Phys. Chem., 97, pp. 13773-13777 | ||
| 504 | |a Lvov, Y., Decher, G., Mohwald, H., (1993) Langmuir, 9, pp. 481-486 | ||
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| 504 | |a Decher, G., Lehr, B., Lowack, K., Lvov, Y., Schmitt, J., (1994) Biosens. Bioelectron., 9, pp. 677-684 | ||
| 504 | |a Lvov, Y., Haas, H., Decher, G., Mohwald, H., Mikhailov, A., Mtchedlishvily, B., Morgunona, E., Vainshtein, B., (1994) Langmuir, 10, pp. 4232-4236 | ||
| 504 | |a Lvov, Y., Ariga, K., Ichinose, I., Kunitake, T., (1995) J. Am. Chem. Soc., 117, pp. 6117-6123 | ||
| 504 | |a Onda, M., Lvov, Y., Ariga, K., Kunitake, T., (1996) Biotechnol. Bioeng., 51, pp. 163-167 | ||
| 504 | |a Hodak, J., Etchenique, R., Calvo, E.J., Singhal, K., Bartlett, P.N., (1997) Langmuir, 13, pp. 2708-2716 | ||
| 504 | |a Laurent, D., Schelenoff, J.B., (1997) Langmuir, 13, pp. 1552-1557 | ||
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| 504 | |a Forzani, E.S., Rivas, G.A., Solis, V.M., (1995) J. Electroanal. Chem., 382, pp. 33-40 | ||
| 504 | |a Forzani, E.S., Rivas, G.A., Solis, V.M., (1997) J. Electroanal. Chem., 435, pp. 77-84 | ||
| 504 | |a Forzani, E.S., Rivas, G.A., Solis, V.M., (1999) J. Electroanal. Chem., 461, pp. 174-183 | ||
| 504 | |a Technical information of Sigma Chemical Co.: Tyrosinase from mushroom, Prod. No. T7755; Calvo, E.J., Etchenique, R., Barttlet, P.N., Singhal, K., Santamaria, C., (1997) Faraday Discuss., 107, pp. 141-157 | ||
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| 504 | |a Forzani, E.S., (1999), Doctoral Thesis, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba; Desprez, V., Labbe, P., (1996) J. Electroanal. Chem., 415, pp. 191-195 | ||
| 520 | 3 | |a We report here a novel bioelectrode based on self-assembled multilayers of polyphenol oxidase intercalated with cationic polyallylamine built up on a thiol-modified gold surface. We use an immobilization strategy previously described by Hodak J. et al. (Langmuir 1997, 13, 2708-2716) Quartz crystal microbalance with electroacustic impedance experiments were carried oat to follow quantitatively the multilayer film formation. The response of the self-assembly polyphenol oxidase-polyallylamine electrodes toward different metabolically related catecholamines was studied, to evaluate enzyme kinetics. For the analyzed compounds, only dopamine and its metabolite Dopac gave catalytic currents at applied potential close to 0 V. These responses were proportional to the number of polyphenol oxidase-immobilized layers and were also controlled by the enzymatic reaction. The combination of microgravimetric and electrochemical techniques allowed us to determine the kinetic enzymatic constants, showing that the decomposition rate for the enzyme-substrate complex is slower than the enzymatic reoxidation step. |l eng | |
| 593 | |a INFIQC, Departamento de Fisicoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Pabellón, Argentina | ||
| 593 | |a INQUIMAE, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Argentina | ||
| 593 | |a AR-1428, Ciudad Universitaria, Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a ALLYLAMINE DERIVATIVE |
| 690 | 1 | 0 | |a CATECHOL OXIDASE |
| 690 | 1 | 0 | |a CATION |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a ELECTROCHEMICAL ANALYSIS |
| 690 | 1 | 0 | |a ELECTROCHEMISTRY |
| 690 | 1 | 0 | |a ELECTRODE |
| 690 | 1 | 0 | |a IMMOBILIZATION |
| 690 | 1 | 0 | |a SURFACE PROPERTY |
| 690 | 1 | 0 | |a CATECHOL OXIDASE |
| 690 | 1 | 0 | |a ELECTROCHEMISTRY |
| 690 | 1 | 0 | |a ENZYMES, IMMOBILIZED |
| 690 | 1 | 0 | |a INDICATORS AND REAGENTS |
| 690 | 1 | 0 | |a POLYAMINES |
| 651 | 4 | |a ARGENTINA | |
| 700 | 1 | |a Solís, V.M. | |
| 700 | 1 | |a Calvo, E.J. | |
| 773 | 0 | |d 2000 |g v. 72 |h pp. 5300-5307 |k n. 21 |p Anal. Chem. |x 00032700 |w (AR-BaUEN)CENRE-146 |t Analytical Chemistry | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034332643&doi=10.1021%2fac0003798&partnerID=40&md5=91de19758cb7f97f2d1f374b55e87ab8 |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1021/ac0003798 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00032700_v72_n21_p5300_Forzani |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v72_n21_p5300_Forzani |y Registro en la Biblioteca Digital |
| 961 | |a paper_00032700_v72_n21_p5300_Forzani |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 963 | |a VARI | ||
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