Are antigen test kits efficient for detecting heartworm-infected dogs at the southern distribution limit of the parasite in South America? Preliminary results
The aim of this study was to evaluate the performance of commercial heartworm antigen tests in dogs harbouring Dirofilaria immitis microfilariae near its distribution limit in South America. A total of 4934 blood samples of adult dogs from Southern Greater Buenos Aires were examined to detect circul...
Guardado en:
| Autor principal: | |
|---|---|
| Otros Autores: | , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
2008
|
| Materias: | |
| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| Sumario: | The aim of this study was to evaluate the performance of commercial heartworm antigen tests in dogs harbouring Dirofilaria immitis microfilariae near its distribution limit in South America. A total of 4934 blood samples of adult dogs from Southern Greater Buenos Aires were examined to detect circulating microfilariae in the buffy coat interface between December 2005 and April 2006. Microfilariae were detected in 88 (1.8%) blood samples and all the microfilariae observed were identified as D. immitis by acid phosphatase stain technique. In a first trial, 69 (78.4%) out of the 88 serum were positive by Speed® Diro. Then, a new test was performed over 25 microfilariae-positive serum samples randomly selected among the 88 previously tested samples and using simultaneously Speed® Diro, Witness® Dirofilaria and Snap® 3dx. This second trial showed identical results for the three different tests, in which 19 (76%) samples were positive. Therefore, more than 20% of microfilaremic dogs were antigen negative. The main hypothesis that could explain our finding is a low worm burden in the study area. According to our preliminary results, it is highly recommendable the complementary use of antigen tests and other procedures to obtain an accurate diagnostic near the distribution limit of the parasite. © 2007 Elsevier Ltd. All rights reserved. |
|---|---|
| Bibliografía: | Aranda, C., Panyella, O., Eritja, R., Castella, J., Canine filariasis. Importance and transmission in the Baix Lobregat area, Barcelona (Spain) (1998) Veterinary Parasitology, 77, pp. 267-275 Atkins, C.E., Comparison of results of three commercial heartworm antigen test kits in dogs with low heartworm burdens (2003) Journal of the American Veterinary Medical Association, 222, pp. 1221-1223 Casiraghi, M., Bazzocchi, C., Mortarino, M., Ottina, E., Genchi, C., A simple molecular method for discriminating common filarial nematodes of dogs (Canis familiaris) (2006) Veterinary Parasitology, 141, pp. 368-372 Chalifoux, L., Hunt, R.D., Histochemical differentiation of Dirofilaria immitis and Dipetalonema reconditum (1971) Journal of the American Veterinary Medical Association, 158, pp. 601-605 Courtney, C.H., Cornell, J.A., Evaluation of heartworm immunodiagnostic tests (1990) Journal of the American Veterinary Medical Association, 197, pp. 724-729 Courtney, C.H., Zeng, Q.Y., Relationship between microfilaria count and sensitivity of the direct smear for diagnosis of canine dirofilariosis (2001) Veterinary Parasitology, 94, pp. 199-204 Genchi, C., Venco, L., Genchi, M., Guideline for the laboratory diagnosis of canine and feline Dirofilaria infections (2007) Mappe Parassitologiche, 8, pp. 139-144 Goodwin, J., The serologic diagnosis of heartworm infection in dogs and cats (1998) Clinical Techniques in Small Animal Practice, 13, pp. 83-87 IGM, 1998. Atlas Geográfico de la República Argentina. Instituto Geográfico Militar, Buenos Aires, 95 pp; INDEC, 2001. Censo Nacional de Población, Hogares y Viviendas 2001. Instituto Nacional de Estadísticas y Censos (INDEC), Ministerio de Economía y Producción, República Argentina; Kaiser, L., Williams, J., Dirofilaria immitis: worm burden and pulmonary artery proliferation in dogs from Michigan (United States) (2004) Veterinary Parasitology, 124, pp. 125-129 Klotins, K.C., Martin, S.W., Bonnett, B.N., Peregrine, A.S., Canine heartworm testing in Canada: are we being effective? (2000) Canadian Veterinary Journal, 41, pp. 929-937 Labbé, J., Castronuovo, A.., Labbé, C., Velásquez, J., 1995. Morbilidad de Dirofilaria immitis en canes de los partidos de Lanus y Lomas de Zamora. In: Primer Congreso Latinoamericano de Zoonosis, Buenos Aires, p. 187; Martini, M., Capelli, G., Poglayen, G., Bertotti, F., Turilli, C., The validity of some haematological and elisa methods for the diagnosis of canine heartworm disease (1996) Veterinary Research Communications, 20, pp. 331-339 Miller, M.W., Canine heartworm disease (1998) Clinical Techniques in Small Animal Practice, 13, pp. 113-118 Nelson, C.T., McCall, J.W., Rubin, S.B., Buzhardt, L.F., Doiron, D.W., Graham, W., Longhofer, S.L., Paul, A., 2005 Guidelines for the diagnosis, prevention and management of heartworm (Dirofilaria immitis) infection in dogs (2005) Veterinary Parasitology, 133, pp. 255-266 Rishniw, M., Barr, S.C., Simpson, K.W., Frongillo, M.F., Franz, M., Alpizar, J.L.D., Discrimination between six species of canine microfilariae by a single polymerase chain reaction (2006) Veterinary Parasitology, 135, pp. 303-314 Tarello, W., 2001. Importance in the dog of concentration tests for the diagnosis of heartworm disease in non-endemic areas. Vet On-Line 2, available at <http://www.priory.com/vet/cardioworm.htm/>; Vezzani, D., Carbajo, A.E., Spatial and temporal transmission risk of Dirofilaria immitis in Argentina (2006) International Journal for Parasitology, 26, pp. 1463-1472 Vezzani, D., Eiras, D.F., Wisnivesky, C., Dirofilariasis in Argentina: historical review and first report of Dirofilaria immitis in a natural mosquito population (2006) Veterinary Parasitology, 136, pp. 259-273 |
| ISSN: | 00345288 |
| DOI: | 10.1016/j.rvsc.2007.08.001 |