Glucocorticoid-induced apoptosis: A simple set of laboratory experiments
Here, we present a laboratory experiment for students of human biology and medicine. In this experiment the students have the opportunity of measuring one of the hallmarks of programmed cell death, which is DNA fragmentation. The biological system that we examine here is the glucocorticoid-induced a...
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2000
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| LEADER | 06567caa a22007577a 4500 | ||
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| 001 | PAPER-2207 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203136.0 | ||
| 008 | 190411s2000 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0034492971 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 100 | 1 | |a Vicent, G.P. | |
| 245 | 1 | 0 | |a Glucocorticoid-induced apoptosis: A simple set of laboratory experiments |
| 260 | |c 2000 | ||
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Majno, G., Joris, I., Apoptosis, oncosis, and necrosis, an overview of cell death (1995) Amer. J. Pathol., 146 (1), pp. 3-15 | ||
| 504 | |a Wyllie, A.H., Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation (1980) Nature, 284 (5756), pp. 555-556 | ||
| 504 | |a Oldenburg, N.B., Evans-Storms, R.B., Cidlowski, J.A., In vivo resistance to glucocorticoid-induced apoptosis in rat thymocytes with normal steroid receptor function in vitro (1997) Endocrinology, 138 (2), pp. 810-818 | ||
| 504 | |a Raff, M., Cell suicide for beginners (1998) Nature, 396 (6707), pp. 119-122 | ||
| 504 | |a Beato, M., Herrlich, P., Schutz, G., Steroid hormone receptors; many actors in search of a plot (1995) Cell, 83 (6), pp. 851-857 | ||
| 504 | |a Vicent, G.P., Monteserin, M.C., Veleiro, A.S., Burton, G., Lantos, C.P., Galigniana, M.D., 21-Hydroxy-6, 19-oxidoprogesterone; a novel synthetic steroid with specific antiglucocorticoid properties in the rat (1997) Molecular Pharmacol., 52 (4), pp. 749-753 | ||
| 504 | |a Agarwal, A.K., The antiglucocorticoid action of mifepristone (1996) Pharmacol. Therap., 70 (3), pp. 183-213 | ||
| 504 | |a Nilsson, B., Ferno, M., von Schoultz, B., Estrogen and progesterone receptors in the human thymus (1990) Gynecol. Obst. Invest., 29 (4), pp. 289-291 | ||
| 504 | |a Bottenstein, J., Hayashi, I., Hutchings, S., Masui, H., Mather, J., McClure, D.B., Ohasa, S., Wu, R., The growth of cells in serum-free hormone-supplemented media (1979) Meth. Enzymol., 58, pp. 94-109 | ||
| 504 | |a Reul, J.M., Pearce, P.T., Funder, J.W., Krozowski, Z.S., Type I and type II corticosteroid receptor gene expression in the rat; effect of adrenalectomy and dexamethasone administration (1989) Molecular Endocrinol., 3 (10), pp. 1674-1680 | ||
| 504 | |a Isohashi, F., Tsukanaka, K., Terada, M., Nakanishi, Y., Tani, S., Sakamoto, Y., Restoration by adrenalectomy of weight and binding capacity of glucorcorticoid receptors of the thymus in AH 130 tumor-bearing rats (1978) Cancer Res., 38, pp. 4243-4245 | ||
| 504 | |a Canadian Council on Animal Care (Eds.), Guide to the Care and Use of Experimental Animals, Otawa, Ontario, 1984; Herrmann, M., Lorenz, H.M., Voll, R., Grunke, M., Woith, W., Kalden, J.R., Arapid and simple method for the isolation of apoptotic DNA fragments (1994) Nucl. Acids Res., 22 (24), pp. 5506-5507 | ||
| 504 | |a Vicent, G.P., Pecci, A., Ghini, A., Piwien-Pilipuk, G., Veleiro, A.S., Burton, G., Lantos, C.P., Galigniana, M.D., The glucocorticoid properties of the synthetic steroid pregnan-1, 4-diene-11b-ol-3, 20-dione (DHOP) are not enterely correlated with the steroid binding to the glucocorticoid receptor (1999) Mol. Cell. Endocrinol., 149, pp. 207-219 | ||
| 504 | |a Vicent, G.P., Burton, G., Ghini, A., Lantos, C.P., Galigniana, M.D., Influence of calf serum on glucocorticoid-responses of certain progesterone derivatives (1998) J. Steroid Biochem. Mol. Biol., 66 (4), pp. 211-216 | ||
| 504 | |a Galigniana, M.D., Vicent, G.P., Burton, G., Lantos, C.P., Features of the shuttle pair beta 11-hydroxyprogesterone-11-ketoprogesterone (1997) Steroids, 62 (4), pp. 358-364 | ||
| 504 | |a Cidlowski, J.A., King, K.L., Evans-Storms, R.B., Montague, J.W., Bortner, C.D., Hughes, F.M.J., The biochemistry and molecular biology of glucocorticoid-induced apoptosis in the immune system (1996) Recent Prog. Hormone Res., 51, pp. 457-490 | ||
| 504 | |a Evans-Storms, R.B., Cidlowski, J.A., Regulation of apoptosis by steroid hormones (1995) J. Steroid Biochem. Mol. Biol., 53 (1-6), pp. 1-8 | ||
| 520 | 3 | |a Here, we present a laboratory experiment for students of human biology and medicine. In this experiment the students have the opportunity of measuring one of the hallmarks of programmed cell death, which is DNA fragmentation. The biological system that we examine here is the glucocorticoid-induced apoptosis in the thymus. The set of experiments that we propose to perform and discuss are relatively simple and can be rapidly carried out in a course for undergraduates. This practice also allows the students to familiarize themselves with basic pharmacology, compare in vivo vs. in vitro assays and acquire skills in animal handling. The organisation of the practical work requires 4-5h of experimental training in each of two days. Copyright © 2001 IUBMB. |l eng | |
| 593 | |a Departamento De Química Biológica, Facultad De Ciencias Exactas Y Naturales, Universidad De Buenos Aires Y PRHOM (CONICET), Pabellón II Ciudad Universitaria, 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a CORTICOSTERONE |
| 690 | 1 | 0 | |a DEXAMETHASONE |
| 690 | 1 | 0 | |a DNA FRAGMENT |
| 690 | 1 | 0 | |a GLUCOCORTICOID |
| 690 | 1 | 0 | |a GLUCOCORTICOID RECEPTOR |
| 690 | 1 | 0 | |a MIFEPRISTONE |
| 690 | 1 | 0 | |a PROGESTERONE |
| 690 | 1 | 0 | |a TESTOSTERONE |
| 690 | 1 | 0 | |a ANIMAL CELL |
| 690 | 1 | 0 | |a APOPTOSIS |
| 690 | 1 | 0 | |a CONCENTRATION RESPONSE |
| 690 | 1 | 0 | |a CONFERENCE PAPER |
| 690 | 1 | 0 | |a DRUG EFFECT |
| 690 | 1 | 0 | |a MALE |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a RAT |
| 690 | 1 | 0 | |a THYMOCYTE |
| 690 | 1 | 0 | |a THYMUS |
| 690 | 1 | 0 | |a ANIMALIA |
| 653 | 0 | 0 | |a ru 38486, Sigma, United States |
| 700 | 1 | |a Lanuza, G.M. | |
| 700 | 1 | |a Romero, D.G. | |
| 700 | 1 | |a Di Croce, L. | |
| 700 | 1 | |a Pecci, A. | |
| 773 | 0 | |d 2000 |g v. 28 |h pp. 307-312 |k n. 6 |p Biochem. Mol. Biol. Educ. |x 14708175 |w (AR-BaUEN)CENRE-1893 |t Biochemistry and Molecular Biology Education | |
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| 856 | 4 | 0 | |u https://doi.org/10.1016/S1470-8175(00)00025-4 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_14708175_v28_n6_p307_Vicent |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_14708175_v28_n6_p307_Vicent |y Registro en la Biblioteca Digital |
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