Preliminary characterization of bacteriocins from Lactococcus lactis, Enterococcus faecium and Enterococcus mundtii strains isolated from turbot (Psetta maxima)

The aim of this work was the characterization of new strains of lactic acid bacteria (LAB) from farmed fish and with potential application as biopreservatives against both Listeria monocytogenes and Staphylococcus aureus. Twenty-five strains of LAB isolated from the muscle of farmed turbot were inve...

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Autor principal: Campos, C.A
Otros Autores: Rodríguez, O., Calo-Mata, P., Prado, M., Barros-Velázquez, J.
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2006
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030 |a FORIE 
100 1 |a Campos, C.A. 
245 1 0 |a Preliminary characterization of bacteriocins from Lactococcus lactis, Enterococcus faecium and Enterococcus mundtii strains isolated from turbot (Psetta maxima) 
260 |c 2006 
270 1 0 |m Barros-Velázquez, J.; Department of Analytical Chemistry, Nutrition and Food Science, School of Veterinary Sciences, University of Santiago de Compostela, E-27002 Lugo, Spain; email: jbarros@lugo.usc.es 
506 |2 openaire  |e Política editorial 
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504 |a Ben-Gigirey, B., Vieites-Baptista De Sousa, J.M., Villa, T.G., Barros-Velázquez, J., Characterization of biogenic amine-producing Stenotrophomonas maltophilia strains isolated from white muscle of fresh and frozen albacore tuna (2000) International Journal of Food Microbiology, 57, pp. 19-31 
504 |a Blom, H., Katia, T., Nissen, H., Holo, H., Characterization, production and purification of carnocin H, a bacteriocin produced by Carnobacterium 377 (2001) Current Microbiology, 43, pp. 227-231 
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504 |a Coventry, M.J., Gordon, J.B., Wilcock, A., Amarc, K., Davidson, B.E., Hickey, M.W., Detection of bacteriocins of lactic acid bacteria isolated from foods and comparison with pediocin and nisin (1997) Journal of Applied Microbiology, 83, pp. 248-258 
504 |a Duffes, F., Leroi, F., Boyaval, P., Dousset, X., Inhibition of Listeria monocytogenes by Carnobacterium spp strains in a simulated cold smoked fish system stored at 4°C (1999) International Journal of Food Microbiology, 47, pp. 33-42 
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504 |a Huss, H.H., Jeppesen, V.F., Johansen, C., Gram, L., Biopreservation of fish products. a review of recent approaches and results (1995) Journal of Aquatic Food Products Technology, 42, pp. 5-26 
504 |a Lee, H.J., Joo, Y.L., Park, C.H., Kim, S.H., Hwang, I., Ahn, J.G., Purification and characterization of a bacteriocin produced by L. lactis subsp. lactis H559 isolated from kimchi (1999) Journal of Bioscience and Bioengineering, 88, pp. 153-159 
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504 |a Lyhs, U., Björkroth, J., Korkeala, H., Characterization of lactic acid bacteria from spoiled vacuum-packaged, cold-smoked rainbow trout using ribotyping (1999) International Journal of Food Microbiology, 52, pp. 77-84 
504 |a Marrug, J.D., Bacteriocins, their role in developing natural products (1991) Food Biotechnology, 5, pp. 305-312 
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504 |a Pilet, M.F., Dousset, X., Barre, R., Novel, G., Desmazeaud, M., Piard, J.C., Evidence for two bacteriocins produced by Carnobacterium piscicola and Carnobacterium divergens isolated from fish and active against Listeria monocytogenes (1995) Journal of Food Protection, 58, pp. 256-262 
504 |a Ringøí, E., Gatesoupe, F., Lactic acid bacteria: A review (1998) Aquaculture, 160, pp. 177-203 
504 |a Rodriguez, O., Velázquez, J.B., Ojea, A., Piñeiro, C., Aubourg, S.P., Evaluation of sensory and microbiological changes and identification of proteolytic bacteria during the iced storage of farmed turbot (Psetta maxima) (2003) Journal of Food Science, 68, pp. 2764-2770 
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504 |a Velázquez, J.B., Jiménez, A., Chomón, B., Villa, T.G., Incidence and transmission of antibiotic resistance in Campylobacter jejuni and Campylobacter coli strains isolated in Spain (1995) Journal of Antimicrobial Chemotherapy, 35, pp. 173-178 
504 |a Yamazaki, K., Suzuki, M., Kawai, Y., Inque, N., Montville, T., Inhibition of Listeria monocytogenes in cold-smoked salmon by Carnobacterium piscicola CS526 isolated from frozen surimi (2003) Journal of Food Protection, 66, pp. 1420-1425 
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520 3 |a The aim of this work was the characterization of new strains of lactic acid bacteria (LAB) from farmed fish and with potential application as biopreservatives against both Listeria monocytogenes and Staphylococcus aureus. Twenty-five strains of LAB isolated from the muscle of farmed turbot were investigated. Genetic identification of the bacteriocin-producing LAB strains was performed by means of a PCR method using novel BAL1/BAL2 16S ribosomal-RNA-targeted primers. Maximum bacteriocin production by Lactococcus lactis ssp. lactis USC-39, Enterococcus faecium USC-46 and Enterococcus mundtii USC-51 was detected in the stationary phase of growth. Both acidification and the production of hydrogen peroxide by LAB were ruled out as the source of the inhibition. In contrast, the antimicrobial activity of all three LAB strains was inactivated by the addition of proteinase K, thus confirming the proteinaceous nature of the inhibition. The activity against L. monocytogenes was maintained in the 3.5-5.5 or 3.5-6.5 pH range, depending on the LAB strain. Likewise, inhibition of S. aureus strains was observed in the 3.5-4.5 and in the 3.5-5.5 pH ranges, depending on the LAB strain and on the S. aureus strain tested. Bacteriocin activity was stable in all three strains after heating the cell-free extract for 60 min at 100°C, or even for 15 min at 121°C, in all the three LAB strains. The acidic and heat-resistant bacteriocins produced by the three LAB strains isolated from turbot, able to inhibit the growth of both L. monocytogenes and S. aureus may find application as biopreservatives in fermented and/or heated food products. © 2005 Elsevier Ltd. All rights reserved.  |l eng 
536 |a Detalles de la financiación: Xunta de Galicia, PGIDT01MAR40202PR 
536 |a Detalles de la financiación: University of South China 
536 |a Detalles de la financiación: Ministerio de Asuntos Exteriores y de Cooperación, MAEC 
536 |a Detalles de la financiación: This work was supported by a project granted by the Secretaría Xeral de I + D from the Xunta de Galicia (Project PGIDT01MAR40202PR). The authors acknowledge the financial assistance of a grant from the Ministerio de Asuntos Exteriores of Spain which supported Dr. C.A. Campos visit to our laboratory at USC. The authors want to thank Dr. F. Barros for his cooperation in the sequencing studies. 
593 |a Department of Industries, University of Buenos Aires, Ciudad Universitaria, (1428) Buenos Aires, Argentina 
593 |a Department of Analytical Chemistry, Nutrition and Food Science, School of Veterinary Sciences, University of Santiago de Compostela, E-27002 Lugo, Spain 
593 |a College of Biotechnology, Institute of Aquaculture, University of Santiago de Compostela, E-15782 Santiago, Spain 
690 1 0 |a BACTERIOCIN 
690 1 0 |a BIOPRESERVATION 
690 1 0 |a ENTEROCOCCUS 
690 1 0 |a FISH 
690 1 0 |a LAB 
690 1 0 |a LACTOCOCCUS 
690 1 0 |a LISTERIA MONOCYTOGENES 
690 1 0 |a STAPHYLOCOCCUS AUREUS 
690 1 0 |a CARBOXYLIC ACIDS 
690 1 0 |a ENZYME INHIBITION 
690 1 0 |a FERMENTATION 
690 1 0 |a FISHERIES 
690 1 0 |a FOOD PRODUCTS 
690 1 0 |a PH EFFECTS 
690 1 0 |a BACTERIOCIN 
690 1 0 |a BIOPRESERVATION 
690 1 0 |a ENTEROCOCCUS 
690 1 0 |a FISH 
690 1 0 |a LAB 
690 1 0 |a LACTOCOCCUS 
690 1 0 |a LISTERIA MONOCYTOGENES 
690 1 0 |a STAPHYLOCOCCUS AUREUS 
690 1 0 |a BACTERIA 
690 1 0 |a BACTERIA (MICROORGANISMS) 
690 1 0 |a ENTEROCOCCUS 
690 1 0 |a ENTEROCOCCUS FAECIUM 
690 1 0 |a ENTEROCOCCUS MUNDTII 
690 1 0 |a LACTOCOCCUS 
690 1 0 |a LACTOCOCCUS LACTIS 
690 1 0 |a LISTERIA MONOCYTOGENES 
690 1 0 |a SCOPHTHALMUS MAXIMUS 
690 1 0 |a STAPHYLOCOCCUS AUREUS 
650 1 7 |2 spines  |a GENES 
700 1 |a Rodríguez, O. 
700 1 |a Calo-Mata, P. 
700 1 |a Prado, M. 
700 1 |a Barros-Velázquez, J. 
773 0 |d 2006  |g v. 39  |h pp. 356-364  |k n. 3  |p Food Res. Int.  |x 09639969  |w (AR-BaUEN)CENRE-1760  |t Food Research International 
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