Decreased protein tyrosine phosphorylation and membrane fluidity in spermatozoa from infertile men with varicocele
Varicocele is a prevalent pathology among infertile men. The mechanisms linking this condition to infertility, however, are poorly understood. Our previous work showed a relationship between sperm functional quality and the ability of spermatozoa to respond to capacitating conditions with increased...
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2006
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| LEADER | 19861caa a22017057a 4500 | ||
|---|---|---|---|
| 001 | PAPER-21711 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518205311.0 | ||
| 008 | 190411s2006 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-33750312315 | |
| 024 | 7 | |2 cas |a protein tyrosine kinase, 80449-02-1; Protein-Tyrosine Kinases, EC 2.7.1.112 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a MREDE | ||
| 100 | 1 | |a Buffone, M.G. | |
| 245 | 1 | 0 | |a Decreased protein tyrosine phosphorylation and membrane fluidity in spermatozoa from infertile men with varicocele |
| 260 | |c 2006 | ||
| 270 | 1 | 0 | |m Doncel, G.F.; CONRAD Program, Department of Obstetrics, Eastern Virginia Medical School, 601 Colley Ave, Norfolk, VA 23507, United States; email: DoncelGF@evms.edu |
| 506 | |2 openaire |e Política editorial | ||
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| 520 | 3 | |a Varicocele is a prevalent pathology among infertile men. The mechanisms linking this condition to infertility, however, are poorly understood. Our previous work showed a relationship between sperm functional quality and the ability of spermatozoa to respond to capacitating conditions with increased membrane fluidity and protein tyrosine phosphorylation. Given the reported association between varicocele, oxidative stress, and sperm dysfunction, we hypothesized that spermatozoa from infertile patients with varicocele might have a combined defect at the level of membrane fluidity and protein tyrosine phosphorylation. Semen samples from infertile patients with and without grade II/III left varicocele were evaluated for motion parameters (computer-assisted semen analysis [CASA]), hyperactivation (CASA), incidence and intensity of protein tyrosine phosphorylation (phosphotyrosine immunofluorescence and western blotting), and membrane fluidity (Laurdan fluorometry), before and after a capacitating incubation (6 hr at 37°C in Ham's F10/BSA, 5% CO2). Spermatozoa from varicocele samples presented a decreased response to the capacitating challenge, showing significantly lower motility, hyperactivation, incidence and intensity of tyrosine phosphorylation, and membrane fluidity. The findings reported in this article indicate that the sperm dysfunction associated to infertile varicocele coexists with decreased sperm plasma membrane fluidity and tyrosine phosphorylation. These deficiencies represent potential new pathophysiological mechanisms underlying varicocele-related infertility. © 2006 Wiley-Liss, Inc. |l eng | |
| 593 | |a Laboratorio de Estudios en Reproducción (LER), Buenos Aires, Argentina | ||
| 593 | |a Centro de Estudios en Ginecología y Reproducción (CEGYR), Buenos Aires, Argentina | ||
| 593 | |a Departamento de Química Biológica, IQUIFIB (CONICET-UBA), Universidad de Buenos Aires, Buenos Aires, Argentina | ||
| 593 | |a Servicio de Urología, Hospital Francés, Buenos Aires, Argentina | ||
| 593 | |a Department of Obstetrics, Jones Institute for Reproductive Medicine, Eastern Virginia Medical School, Norfolk, VA, United States | ||
| 593 | |a CONRAD Program, Department of Obstetrics, Eastern Virginia Medical School, 601 Colley Ave, Norfolk, VA 23507, United States | ||
| 690 | 1 | 0 | |a CAPACITATION |
| 690 | 1 | 0 | |a HUMAN SPERMATOZOA |
| 690 | 1 | 0 | |a HYPERACTIVATION |
| 690 | 1 | 0 | |a MEMBRANE PROPERTIES |
| 690 | 1 | 0 | |a SEMEN PATHOLOGY |
| 690 | 1 | 0 | |a SIGNAL TRANSDUCTION |
| 690 | 1 | 0 | |a PROTEIN TYROSINE KINASE |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CELL MEMBRANE FLUIDITY |
| 690 | 1 | 0 | |a CLINICAL ARTICLE |
| 690 | 1 | 0 | |a COMPUTER ANALYSIS |
| 690 | 1 | 0 | |a ENZYME PHOSPHORYLATION |
| 690 | 1 | 0 | |a HUMAN |
| 690 | 1 | 0 | |a HUMAN CELL |
| 690 | 1 | 0 | |a IMMUNOFLUORESCENCE |
| 690 | 1 | 0 | |a INCIDENCE |
| 690 | 1 | 0 | |a MALE |
| 690 | 1 | 0 | |a MALE INFERTILITY |
| 690 | 1 | 0 | |a PATHOPHYSIOLOGY |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a SEMEN ANALYSIS |
| 690 | 1 | 0 | |a SPERMATOZOON |
| 690 | 1 | 0 | |a SPERMATOZOON CAPACITATION |
| 690 | 1 | 0 | |a SPERMATOZOON MOTILITY |
| 690 | 1 | 0 | |a VARICOCELE |
| 690 | 1 | 0 | |a WESTERN BLOTTING |
| 690 | 1 | 0 | |a ADULT |
| 690 | 1 | 0 | |a CELL MEMBRANE |
| 690 | 1 | 0 | |a HUMANS |
| 690 | 1 | 0 | |a INFERTILITY, MALE |
| 690 | 1 | 0 | |a MALE |
| 690 | 1 | 0 | |a MEMBRANE FLUIDITY |
| 690 | 1 | 0 | |a PHOSPHORYLATION |
| 690 | 1 | 0 | |a PROTEIN-TYROSINE KINASES |
| 690 | 1 | 0 | |a SPERM MOTILITY |
| 690 | 1 | 0 | |a SPERMATOZOA |
| 690 | 1 | 0 | |a VARICOCELE |
| 700 | 1 | |a Brugo-Olmedo, S. | |
| 700 | 1 | |a Calamera, J.C. | |
| 700 | 1 | |a Verstraeten, S.V. | |
| 700 | 1 | |a Urrutia, F. | |
| 700 | 1 | |a Grippo, L. | |
| 700 | 1 | |a Corbetta, J.P. | |
| 700 | 1 | |a Doncel, G.F. | |
| 773 | 0 | |d 2006 |g v. 73 |h pp. 1591-1599 |k n. 12 |p Mol. Reprod. Dev. |x 1040452X |t Molecular Reproduction and Development | |
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