A refined molecular karyotype for the reference strain of the Trypanosoma cruzi genome project (clone CL Brener) by assignment of chromosome markers
We present a useful refinement of the molecular karyotype of clone CL Brener, the reference clone of the Trypanosoma cruzi Genome Project. The assignment of 210 genetic markers (142 expressed sequence tags (ESTs), seven cDNAs, 32 protein-coding genes, eight sequence tagged sites (STSs), 21 repetitiv...
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Elsevier
2003
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| 100 | 1 | |a Porcile, P.E. | |
| 245 | 1 | 2 | |a A refined molecular karyotype for the reference strain of the Trypanosoma cruzi genome project (clone CL Brener) by assignment of chromosome markers |
| 260 | |b Elsevier |c 2003 | ||
| 270 | 1 | 0 | |m Da Silveira, J.F.; Depto. Microbiol., Imunologia P., Escola Paulista de Medicina, UNIFESP, R. Botucatu 862, CEP 04023-062 São Paulo, Brazil; email: franco@ecb.epm.br |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Tibayrenc, M., Ward, P., Moya, A., Ayala, F.J., Natural populations of Trypanosoma cruzi, the agent of Chagas disease, have a complex multi-clonal structure (1986) Proc. Natl. Acad. Sci. USA, 83, pp. 115-119 | ||
| 504 | |a Ullu, E., Tschudi, C., Günzl, A., Trans-splicing in trypanosomatid protozoa (1996) Molecular Biology of Parasitic Protozoa, pp. 115-119. , D.F. Smith, & M. Parsons. Oxford: IRL Press | ||
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| 504 | |a Wincker, P., Murto-Dovales, A.C., Goldenberg, S., Nucleotide sequence of a representative member of a Trypanosoma cruzi dispersed gene family (1992) Mol. Biochem. Parasitol., 55, pp. 217-220 | ||
| 520 | 3 | |a We present a useful refinement of the molecular karyotype of clone CL Brener, the reference clone of the Trypanosoma cruzi Genome Project. The assignment of 210 genetic markers (142 expressed sequence tags (ESTs), seven cDNAs, 32 protein-coding genes, eight sequence tagged sites (STSs), 21 repetitive sequences) to the chromosomal bands separated by pulsed field gel electrophoresis (PFGE) identified 61 chromosome-specific markers, two size-polymorphic chromosomes and seven linkage groups. Fourteen new repetitive elements were isolated in this work and mapped to the chromosomal bands. We found that at least ten repetitive elements can be mapped to each chromosomal band, which may render the whole genome sequence assembly a difficult task. To construct the integrated map of chromosomal band XX, we used yeast artificial chromosome (YAC) overlapping clones and a variety of probes (i.e. known gene sequences, ESTs, STSs generated from the YAC ends). The total length covered by the YAC contig was approximately 1.3 Mb, covering 37% of the entire chromosome. We found some degree of polymorphism among YACs derived from band XX. These results are in agreement with data from phylogenetic analysis of T. cruzi which suggest that clone CL Brener is a hybrid genotype [Mol. Biochem. Parasitol. 92 (1998) 253; Proc. Natl. Acad. Sci. USA 98 (2001) 7396]. The physical map of the chromosomal bands, together with the isolation of specific chromosomal markers, will contribute in the global effort to sequence the nuclear genome of this parasite. © 2003 Elsevier Science B.V. All rights reserved. |l eng | |
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires | ||
| 536 | |a Detalles de la financiación: Fundação de Amparo à Pesquisa do Estado de São Paulo | ||
| 536 | |a Detalles de la financiación: Howard Hughes Medical Institute | ||
| 536 | |a Detalles de la financiación: Fondo para la Investigación Científica y Tecnológica | ||
| 536 | |a Detalles de la financiación: This work was supported by FAPESP (Brazil), CNPq/PADCT (Brazil), FONCYT- SecCyt – University of Buenos Aires (Argentina), CYTED – Subprogram of Biotechnology (Spain), WHO/Special Program for Research and Training in Tropical Diseases. The research of M.J.L. was supported in part by an International Research Scholars grant from the Howard Hughes Medical Institute. | ||
| 593 | |a Depto. Microbiol., Imunologia P., Escola Paulista de Medicina, UNIFESP, R. Botucatu 862, CEP 04023-062 São Paulo, Brazil | ||
| 593 | |a Deto. de Bioquim. e Biol. Molec., FIOCRUZ, Av. Brasil 4365, CEP 21.045-900 Rio de Janeiro, Brazil | ||
| 593 | |a Departamento de Medicina Tropical, FIOCRUZ, Av. Brasil 4365, CEP 21.045-900 Rio de Janeiro, Brazil | ||
| 593 | |a Inst. de Biofis. Carlos Chagas Filho, Univ. Federal do Rio de Janeiro, CEP 21949-900 Rio de Janeiro, Brazil | ||
| 593 | |a Inst. Invest. Ing. Genet. Biol. M., Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a CHROMOSOME SPECIFIC MARKER |
| 690 | 1 | 0 | |a EXPRESSED SEQUENCE TAG |
| 690 | 1 | 0 | |a LINKAGE GROUP |
| 690 | 1 | 0 | |a REPETITIVE SEQUENCE |
| 690 | 1 | 0 | |a YEAST ARTIFICIAL CHROMOSOME CONTIG |
| 690 | 1 | 0 | |a COMPLEMENTARY DNA |
| 690 | 1 | 0 | |a CONTIG |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CHROMOSOMAL LOCALIZATION |
| 690 | 1 | 0 | |a CHROMOSOME BAND |
| 690 | 1 | 0 | |a CHROMOSOME MARKER |
| 690 | 1 | 0 | |a EXPRESSED SEQUENCE TAG |
| 690 | 1 | 0 | |a GENE MAPPING |
| 690 | 1 | 0 | |a GENE SEQUENCE |
| 690 | 1 | 0 | |a GENETIC CODE |
| 690 | 1 | 0 | |a GENETIC LINKAGE |
| 690 | 1 | 0 | |a GENETIC MARKER |
| 690 | 1 | 0 | |a GENOME |
| 690 | 1 | 0 | |a KARYOTYPE |
| 690 | 1 | 0 | |a MOLECULAR CLONING |
| 690 | 1 | 0 | |a MOLECULAR PHYLOGENY |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a NUCLEOTIDE REPEAT |
| 690 | 1 | 0 | |a NUCLEOTIDE SEQUENCE |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a PULSED FIELD GEL ELECTROPHORESIS |
| 690 | 1 | 0 | |a SEQUENCE TAGGED SITE |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a UNINDEXED SEQUENCE |
| 690 | 1 | 0 | |a YEAST ARTIFICIAL CHROMOSOME |
| 690 | 1 | 0 | |a TRYPANOSOMA |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 700 | 1 | |a Santos, M.R.M. | |
| 700 | 1 | |a Souza, R.T. | |
| 700 | 1 | |a Verbisck, N.V. | |
| 700 | 1 | |a Brandão, A. | |
| 700 | 1 | |a Urmenyi, T. | |
| 700 | 1 | |a Silva, R. | |
| 700 | 1 | |a Rondinelli, E. | |
| 700 | 1 | |a Lorenzi, H. | |
| 700 | 1 | |a Levin, M.J. | |
| 700 | 1 | |a Degrave, W. | |
| 700 | 1 | |a Da Silveira, J.F. | |
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