Integration of expression vectors into the ribosomal locus of Trypanosoma cruzi
The expression vectors of the protozoan parasite Trypanosoma cruzi pRIBOTEX and pTREX harbor a ribosomal promoter that improves gene expression and clone selection. Interestingly, the solely presence of this 810 bp long sequence leads to the integration of these vectors into the ribosomal locus, eve...
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| Formato: | Capítulo de libro |
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| Publicado: |
Elsevier
2003
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 08213caa a22009137a 4500 | ||
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| 001 | PAPER-21525 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518205300.0 | ||
| 008 | 190411s2003 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0037498106 | |
| 024 | 7 | |2 Molecular Sequence Numbers |a GENBANK: L13926, U89787 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a GENED | ||
| 100 | 1 | |a Lorenzi, H.A. | |
| 245 | 1 | 0 | |a Integration of expression vectors into the ribosomal locus of Trypanosoma cruzi |
| 260 | |b Elsevier |c 2003 | ||
| 270 | 1 | 0 | |m Levin, M.J.; Lab. Biol. Molec. Enfermedad Chagas, INGEBI - CONICET, University of Buenos Aires, Vuelta de Obligado 2490 2P, 1428 Buenos Aires, Argentina; email: mlevin@dna.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Belli, S.I., Chromatin remodeling during the life cycle of trypanosomatids (2000) Int. J. Parasitol., 30, pp. 679-687 | ||
| 504 | |a Cano, M.I., Gruber, A., Vazquez, M., Cortes, A., Levin, M.J., Gonzalez, A., Degrave, W., Ramirez, J.L., Molecular karyotype of clone CL Brener chosen for the Trypanosoma cruzi genome project (1995) Mol. Biochem. Parasitol., 71, pp. 273-278 | ||
| 504 | |a Dietrich, P., Soares, M.B., Affonso, M.H., Floeter-Winter, L.M., The Trypanosoma cruzi ribosomal RNA-encoding gene: Analysis of promoter and upstream intergenic spacer sequences (1993) Gene, 125, pp. 103-107 | ||
| 504 | |a Fernandez, A.P., Nelson, K., Beverley, S.M., Evolution of nuclear ribosomal RNAs in kinetoplastid protozoa: Perspectives on the age and origins of parasitism (1993) Proc. Natl. Acad. Sci. USA, 90, pp. 11608-11612 | ||
| 504 | |a Hecker, H., Betschart, B., Burri, M., Schlimme, W., Functional morphology of trypanosome chromatin (1995) Parasitol. Today, 11, pp. 79-83 | ||
| 504 | |a Kelly, J., Ward, H., Miles, M., Kendall, G., A shuttle vector which facilitates the expression of transfected genes in Trypanosoma cruzi and Leishmania (1992) Nucleic Acids Res., 20, pp. 3963-3969 | ||
| 504 | |a Lin, Y., Keil, R., Mutations affecting RNA polymerase I-stimulated exchange and rDNA recombination in yeast (1991) Genetics, 127, pp. 31-38 | ||
| 504 | |a Martínez-Calvillo, S., Hernández, R., Trypanosoma cruzi ribosomal DNA: Mapping of a putative distal promoter (1994) Gene, 142, pp. 243-247 | ||
| 504 | |a Martínez-Calvillo, S., López, I., Hernández, R., PRIBOTEX expression vector: A pTEX derivative for a rapid selection of Trypanosoma cruzi transfectants (1997) Gene, 199, pp. 71-76 | ||
| 504 | |a Patnaik, P., Fang, X., Cross, G., The region encompassing the procyclic acidic repetitive protein (PARP) gene promoter plays a role in plasmid DNA replication in Trypanosoma brucei (1994) Nucleic Acids Res., 22, pp. 4111-4118 | ||
| 504 | |a Porto, R.M., Rogério Amino, R., Quartim Elias, M.C., Faria, M., Schenkman, S., Histone H1 is phosphorylated in non-replicating and infective forms of Trypanosoma cruzi (2002) Mol. Biochem. Parasitol., 119, pp. 265-271 | ||
| 504 | |a Stewart, S.E., Roeder, G., Transcription by RNA polymerase I stimulates mitotic recombination in Saccharomyces cerevisiae (1989) Mol. Cell. Biol., 8, pp. 3464-3472 | ||
| 504 | |a Tyler-Cross, R., Short, S., Floeter-Winter, L., Buck, G., Transient expression mediated by the Trypanosoma cruzi rRNA promoter (1995) Mol. Biochem. Parasitol., 72, pp. 23-31 | ||
| 504 | |a Vanhamme, L., Pays, E., Control of gene expression in trypanosomes (1995) Microbiol. Rev., 59, pp. 223-240 | ||
| 504 | |a Vázquez, M., Levin, M.J., Functional analysis of the intergenic regions of TcP2b gene loci allowed the construction of an improved Trypanosoma cruzi expression vector (1999) Gene, 239, pp. 217-225 | ||
| 504 | |a Voelkel-Meiman, K., Keil, R., Roeder, G., Recombination-stimulating sequences in yeast ribosomal DNA correspond to sequences regulating transcription by RNA polymerase I (1987) Cell, 48, pp. 1071-1079 | ||
| 504 | |a Wen, L.M., Xu, P., Benegal, G., Carvaho, M.R., Butler, D.R., Buck, G.A., Trypanosoma cruzi: Exogenously regulated gene expression (2001) Exp. Parasitol., 97, pp. 196-204 | ||
| 520 | 3 | |a The expression vectors of the protozoan parasite Trypanosoma cruzi pRIBOTEX and pTREX harbor a ribosomal promoter that improves gene expression and clone selection. Interestingly, the solely presence of this 810 bp long sequence leads to the integration of these vectors into the ribosomal locus, even though circular plasmids are poorly recombinogenic. Initially, it was suggested that a 174 bp long ribosomal-specific repeat element present in the ribosomal promoter region could be responsible for the genetic exchange. On the contrary, we demonstrate that recombination of pTREX occurs within a 86 bp long region located 120 bp downstream the transcription start point (tsp1) of the ribosomal promoter, and it does not depend on the presence of the ribosomal repeat. We also determined that a 291 bp segment encompassing the tsp1 and the 86 bp long recombination region contains all necessary signals to drive transcription and complete recombination into the rRNA locus. Finally, we demonstrate that the integration of pTREX derived plasmids into the nuclear genome occurs within the first 5 h post-transfection, and that non-integrated copies are rapidly degraded. © 2003 Elsevier Science B.V. All rights reserved. |l eng | |
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires, 01/IX-17 | ||
| 536 | |a Detalles de la financiación: Howard Hughes Medical Institute | ||
| 536 | |a Detalles de la financiación: Fondo para la Investigación Científica y Tecnológica, PICT 01-06803 | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: We would like to thank Maria de los Angeles Curto and graduate student Ignacio Schor for their technical assistance. This work was supported by grants from T. cruzi Genome Project of the University of Buenos Aires; Project 01/IX-17; UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases; Proyecto Genoma de T. cruzi of Centro Argentino-Brasileño de Biotecnologı́a (CABBIO), CONICET, and FONCYT project PICT 01-06803. In addition, the work of MJL was partially supported by an International Research Scholar grant from the Howard Hughes Medical Institute, Chevy Chase, MD, USA. | ||
| 593 | |a Lab. Biol. Molec. Enfermedad Chagas, INGEBI - CONICET, University of Buenos Aires, Vuelta de Obligado 2490 2P, 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a EXPRESSION VECTOR |
| 690 | 1 | 0 | |a RECOMBINATION |
| 690 | 1 | 0 | |a RIBOSOMAL PROMOTER |
| 690 | 1 | 0 | |a TRANSFECTION |
| 690 | 1 | 0 | |a PLASMID VECTOR |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a EXPRESSION VECTOR |
| 690 | 1 | 0 | |a GENE LOCATION |
| 690 | 1 | 0 | |a GENE LOCUS |
| 690 | 1 | 0 | |a GENETIC RECOMBINATION |
| 690 | 1 | 0 | |a GENETIC TRANSFECTION |
| 690 | 1 | 0 | |a GENOME |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a NUCLEOTIDE REPEAT |
| 690 | 1 | 0 | |a NUCLEOTIDE SEQUENCE |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a PROMOTER REGION |
| 690 | 1 | 0 | |a RIBOSOME |
| 690 | 1 | 0 | |a RNA RECOMBINATION |
| 690 | 1 | 0 | |a TIME |
| 690 | 1 | 0 | |a TRANSCRIPTION INITIATION SITE |
| 690 | 1 | 0 | |a TRANSCRIPTION REGULATION |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a PROTOZOA |
| 690 | 1 | 0 | |a TRYPANOSOMA |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 700 | 1 | |a Vazquez, M.P. | |
| 700 | 1 | |a Levin, M.J. | |
| 773 | 0 | |d Elsevier, 2003 |g v. 310 |h pp. 91-99 |k n. 1-2 |p Gene |x 03781119 |w (AR-BaUEN)CENRE-4803 |t Gene | |
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