Angiotensin II phosphorylation of extracellular signal-regulated kinases in rat anterior pituitary cells
We studied the effects of ANG II on extracellular signal-regulated kinase (ERK)1/2 phosphorylation in rat pituitary cells. ANG II increased ERK phosphorylation in a time- and concentration-dependent way. Maximum effect was obtained at 5 min at a concentration of 10-100 nM. The effect of 100 nM ANG I...
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2003
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| 001 | PAPER-21461 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518205255.0 | ||
| 008 | 190411s2003 xx ||||fo|||| 00| 0 eng|d | ||
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| 100 | 1 | |a Suárez, C. | |
| 245 | 1 | 0 | |a Angiotensin II phosphorylation of extracellular signal-regulated kinases in rat anterior pituitary cells |
| 260 | |c 2003 | ||
| 270 | 1 | 0 | |m Becu-Villalobos, D.; Inst. de Biol. Med. Exp.-CONICET, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina; email: dbecu@dna.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Kunert-Radek, J., Pawlikowski, M., Angiotensin II stimulation of the rat pituitary tumoral cell proliferation in vitro (1992) Biochem Biophys Res Commun, 183, pp. 27-30 | ||
| 504 | |a Leduc, I., Haddad, P., Giasson, E., Meloche, S., Involvement of a tyrosine kinase pathway in the growth-promoting effects of angiotensin II on aortic smooth muscle cells (1995) Mol Pharmacol, 48, pp. 582-592 | ||
| 504 | |a Lenkei, Z., Nuyt, A.M., Grouselle, D., Corvol, P., Llorens-Cortes, C., Identification of endocrine cell population expressing the AT 1B subtype of angiotensin II receptors in anterior pituitary (1999) Endocrinology, 140, pp. 472-477 | ||
| 504 | |a Luttrell, L.M., Daaka, Y., Lefkowitz, R.J., Regulation of tyrosine kinase cascades by G-protein-coupled receptors (1999) Curr Opin Cell Biol, 11, pp. 177-183 | ||
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| 504 | |a Molloy, C.J., Taylor, D.S., Weber, H., Angiotensin II stimulation of rapid protein tyrosine phosphorylation and protein kinase activation in rat aortic smooth muscle cells (1993) J Biol Chem, 268, pp. 7338-7345 | ||
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| 504 | |a Murasawa, S., Mori, Y., Nozawa, Y., Masaki, H., Maruyama, K., Tsutsumi, Y., Shibasaki, Y., Matsubara, H., Role of calcium-sensitive tyrosine kinase Pyk2/CAKβ/RAFTK in angiotensin II-induced Ras/ERK signaling (1998) Hypertension, 32, pp. 668-675 | ||
| 504 | |a Naftilan, A.J., Pratt, R.E., Eldrige, C.S., Lin, H.L., Dzau, V.J., Angiotensin II induces c-fos expression in smooth muscle cells via transcriptional control (1989) Hypertension, 13, pp. 706-711 | ||
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| 520 | 3 | |a We studied the effects of ANG II on extracellular signal-regulated kinase (ERK)1/2 phosphorylation in rat pituitary cells. ANG II increased ERK phosphorylation in a time- and concentration-dependent way. Maximum effect was obtained at 5 min at a concentration of 10-100 nM. The effect of 100 nM ANG II was blocked by the AT1 antagonist DUP-753, by the phospholipase C (PLC) inhibitor U-73122, and by the MAPK kinase (MEK) antagonist PD-98059. The ANG II-induced increase in phosphorylated (p)ERK was insensitive to pertussis toxin blockade and PKC depletion or inhibition. The effect was also abrogated by chelating intracellular calcium with BAPTA-AM or TMB-8 by depleting intracellular calcium stores with a 30-min pretreatment with EGTA and by pretreatment with herbimycin A and PP1, two c-Src tyrosine kinase inhibitors. It was attenuated by AG-1478, an inhibitor of epidermal growth factor receptor (EGFR) activation. Therefore, in the rat pituitary, the increase of pERK is a Gq- and PLC-dependent process, which involves an increase in intracellular calcium and activation of a c-Src tyrosine kinase, transactivation of the EGFR, and the activation of MEK. Finally, the response of ERK activation by ANG II is altered in hyperplastic pituitary cells, in which calcium mobilization evoked by ANG II is also modified. |l eng | |
| 593 | |a Inst. de Biol. Med. Exp.-CONICET, 1428 Buenos Aires, Argentina | ||
| 593 | |a Inst. de Biol. Med. Exp.-CONICET, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina | ||
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| 700 | 1 | |a Díaz-Torga, G. | |
| 700 | 1 | |a Gonzalez-Iglesias, A. | |
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| 700 | 1 | |a Becu-Villalobos, D. | |
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