Isolation of a Δ7-cholesterol desaturase from Tetrahymena thermophila
Cell-free preparations of Tetrahymena thermophila catalyze the direct desaturation of cholesterol to Δ7-dehydrocholesterol (provitamin D3). The activity was isolated in the microsomal fraction from Tetrahymena homogenates. Δ7-Desaturase activity was stimulated fivefold by the addition of 6 mM ATP. O...
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| Formato: | Capítulo de libro |
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Springer Verlag
2000
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 07253caa a22009977a 4500 | ||
|---|---|---|---|
| 001 | PAPER-21041 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518205228.0 | ||
| 008 | 190411s2000 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0034181755 | |
| 024 | 7 | |2 cas |a adenosine triphosphate, 15237-44-2, 56-65-5, 987-65-5; nicotinamide adenine dinucleotide, 53-84-9; nicotinamide adenine dinucleotide phosphate, 53-59-8; reduced nicotinamide adenine dinucleotide, 58-68-4; reduced nicotinamide adenine dinucleotide phosphate, 53-57-6; Cholesterol, 57-88-5; Culture Media; lathosterol delta-5-dehydrogenase, EC 1.3.3.2; Oxidoreductases Acting on CH-CH Group Donors, EC 1.3.-; Oxidoreductases, EC 1.- | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a AMBID | ||
| 100 | 1 | |a Valcarce, G. | |
| 245 | 1 | 0 | |a Isolation of a Δ7-cholesterol desaturase from Tetrahymena thermophila |
| 260 | |b Springer Verlag |c 2000 | ||
| 270 | 1 | 0 | |m Florin-Christensen, J.; Dept. of Vet. Microbiol./Pathology, Washington State University, Pullman, WA 99164-7040, United States; email: jfc@vetmed.wsu.edu |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Bertram, J., Erwin, J., Isolation of a stearoyl CoA desaturase from Tetrahymena thermophila (1981) J Protozool, 28, pp. 127-131 | ||
| 504 | |a Bligh, E.G., Dyer, W.J., A rapid method of total lipid extraction and purification (1959) Can J Biochem Physiol, 37, pp. 911-917 | ||
| 504 | |a Casabuono, A., Pomilio, A.B., Linear, steroidal and triterpene esters, and steryl glycosides from Festuca argentina (1997) Lipids, 32, pp. 205-209 | ||
| 504 | |a Christensen, S.T., Schousboe, P., Nielsen, D., Sondergaard, G., Rasmussen, L., Cell multiplication in Tetrahymena setosa and Tetrahymena thermophila in synthetic nutrient medium. Effects of ethanol, cholesterol and extracellular medium (1993) Acta Protozool, 32, pp. 151-156 | ||
| 504 | |a Conner, R.L., Mallory, F.B., Landrey, J.R., Lyengar, C.W.L., The convertion of cholesterol to Δ5,7,22 cholestatrien-3β-ol by Tetrahymena pyriformis (1969) J Biol Chem, 244, pp. 2325-2333 | ||
| 504 | |a Conner, R.L., Landrey, J.R., Joseph, J.M., Nes, W.R., The steric requirements for sterol inhibition of tetrahymanol biosynthesis (1978) Lipids, 13, pp. 692-696 | ||
| 504 | |a Conner, R.L., Landrey, J.R., Czarkowski, N., The effect of specific sterols on cell size and fatty acid composition in Tetrahymena pyriformis W (1983) J Protozool, 29, pp. 105-109 | ||
| 504 | |a Ferguson, K.A., Davis, F.M., Conner, R.L., Landrey, J.R., Mallory, F.B., Effect of sterol replacement in vivo on the fatty acid composition of Tetrahymena (1975) J Biol Chem, 250, pp. 6998-7005 | ||
| 504 | |a Guyer, W., Bloch, K., The sterol requirement of Tetrahymena setosa HZ-1 (1983) J Protozool, 30, pp. 625-629 | ||
| 504 | |a Holz G.G., Jr., Conner, R.L., The composition, metabolism and roles of lipids in Tetrahymena (1973) Biology of Tetrahymena, pp. 97-122. , Elliot AM (ed). Dowden, Hutchinson & Ross, Stroudsberg, Pennsylvania | ||
| 504 | |a Koll, M., Erwin, J.A., The effect of dietary sterol on the activity of fatty acid desaturases isolated from Tetrahymena setosa (1990) J Protozool, 37, pp. 229-237 | ||
| 504 | |a Mallory, F.B., Conner, R.L., Dehydrogenation and dealkylation of various sterols by Tetrahymena pyriformis (1971) Lipids, 6, pp. 149-153 | ||
| 504 | |a Nes, W.R., Joseph, J.M., Landrey, J.R., Behzadan, Sh., Conner, R.L., Steric effects at C-20 and C-24 on the metabolism of sterols by Tetrahymena pyriformis (1981) J Lipid Res, 22, pp. 770-777 | ||
| 504 | |a Wilton, D.C., The effect of cholesterol on ubiquinone and tetrahymanol biosynthesis in Tetrahymena pyriformis (1983) Biochem J, 216, pp. 203-206 | ||
| 504 | |a Zander, J.M., Caspi, E., The stereospecificity of the cholesterol Δ22 dehydrogenase of Tetrahymena pyriformis and the origin of the C-22 protons of cholesterol (1970) J Biol Chem, 245, pp. 1082-1087 | ||
| 520 | 3 | |a Cell-free preparations of Tetrahymena thermophila catalyze the direct desaturation of cholesterol to Δ7-dehydrocholesterol (provitamin D3). The activity was isolated in the microsomal fraction from Tetrahymena homogenates. Δ7-Desaturase activity was stimulated fivefold by the addition of 6 mM ATP. Other cofactors assayed, including NAD, NADP, NADH or NADPH, had no significant effect. The activity was found in microsomes prepared from stationary-phase cultures of the ciliate, grown either with or without added cholesterol, thus indicating that it is constitutively expressed in T. thermophila cells. |l eng | |
| 593 | |a Dept. of Indust. Microbiol./B., School of Pharmacy and Biochemistry, University of Buenos Aires, Junin 956 (1113) Buenos Aires, Argentina | ||
| 593 | |a Dept. of Vet. Microbiol./Pathology, Washington State University, Pullman, WA 99164-7040, United States | ||
| 593 | |a Institute of Neuroscience, INEUCI-CONICET, Ciudad Universitaria, 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a ADENOSINE TRIPHOSPHATE |
| 690 | 1 | 0 | |a DELTA 7 CHOLESTEROL DESATURASE |
| 690 | 1 | 0 | |a NICOTINAMIDE ADENINE DINUCLEOTIDE |
| 690 | 1 | 0 | |a NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE |
| 690 | 1 | 0 | |a PROTOZOAL PROTEIN |
| 690 | 1 | 0 | |a REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE |
| 690 | 1 | 0 | |a REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE |
| 690 | 1 | 0 | |a UNCLASSIFIED DRUG |
| 690 | 1 | 0 | |a CHOLESTEROL |
| 690 | 1 | 0 | |a LATHOSTEROL DELTA 5 DEHYDROGENASE |
| 690 | 1 | 0 | |a LATHOSTEROL DELTA-5-DEHYDROGENASE |
| 690 | 1 | 0 | |a OXIDOREDUCTASE |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a BIOTECHNOLOGY |
| 690 | 1 | 0 | |a BIOTRANSFORMATION |
| 690 | 1 | 0 | |a CHEMICAL REACTION KINETICS |
| 690 | 1 | 0 | |a DAIRY INDUSTRY |
| 690 | 1 | 0 | |a ENZYME ACTIVITY |
| 690 | 1 | 0 | |a ENZYME ISOLATION |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a TETRAHYMENA THERMOPHILA |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a CULTURE MEDIUM |
| 690 | 1 | 0 | |a ENZYMOLOGY |
| 690 | 1 | 0 | |a GROWTH, DEVELOPMENT AND AGING |
| 690 | 1 | 0 | |a ISOLATION AND PURIFICATION |
| 690 | 1 | 0 | |a METABOLISM |
| 690 | 1 | 0 | |a MICROSOME |
| 690 | 1 | 0 | |a CILIOPHORA |
| 690 | 1 | 0 | |a PROTOZOA |
| 690 | 1 | 0 | |a TETRAHYMENA THERMOPHILA |
| 690 | 1 | 0 | |a TETRAHYMENA THERMOPHILA |
| 690 | 1 | 0 | |a ANIMALS |
| 690 | 1 | 0 | |a CHOLESTEROL |
| 690 | 1 | 0 | |a CULTURE MEDIA |
| 690 | 1 | 0 | |a MICROSOMES |
| 690 | 1 | 0 | |a OXIDOREDUCTASES |
| 690 | 1 | 0 | |a OXIDOREDUCTASES ACTING ON CH-CH GROUP DONORS |
| 690 | 1 | 0 | |a TETRAHYMENA THERMOPHILA |
| 700 | 1 | |a Florin-Christensen, J. | |
| 700 | 1 | |a Nudel, C. | |
| 773 | 0 | |d Springer Verlag, 2000 |g v. 53 |h pp. 591-595 |k n. 5 |p Appl. Microbiol. Biotechnol. |x 01757598 |w (AR-BaUEN)CENRE-173 |t Applied Microbiology and Biotechnology | |
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| 856 | 4 | 0 | |u https://doi.org/10.1007/s002530051661 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_01757598_v53_n5_p591_Valcarce |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01757598_v53_n5_p591_Valcarce |y Registro en la Biblioteca Digital |
| 961 | |a paper_01757598_v53_n5_p591_Valcarce |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 81994 | ||