An improved microbial synthesis of purine nucleosides

E. coli BL21 synthesized purine nucleosides from pyrimidine ones. A 94% yield of adenosine from uridine was reached within 1 h.

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Autor principal: Lewkowicz, E.S
Otros Autores: Martínez, N., Rogert, M.C, Porro, S., Iribarren, A.M
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2000
Acceso en línea:Registro en Scopus
DOI
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
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100 1 |a Lewkowicz, E.S. 
245 1 3 |a An improved microbial synthesis of purine nucleosides 
260 |c 2000 
270 1 0 |m Lewkowicz, E.S.; Universidad Nacional de Quilmes, R. S. Pena 180, (1876) Bernal, Buenos Aires, Argentina; email: elewko@unq.ed.ar 
506 |2 openaire  |e Política editorial 
504 |a Agrawal, S., Iyer, R.P., Modified oligonucleotides as therapeutic and diagnostic agents (1995) Curr. Opin. Biotech., 6, pp. 12-19 
504 |a Hanrahan, J., Hutchinson, D., The enzymatic synthesis of antiviral agents (1992) J. Biotechnol., 23, pp. 193-210 
504 |a Iribarren, A.M., Sproat, B.S., Neuner, P., Sulston, I., Ryder, U., Lamond, A.I., 2'-O-alkyl oligoribonucleotides as antisense probes (1990) Proc. Natl. Acad. Sci. USA, 87, pp. 7747-7751 
504 |a Krenitzky, T., Koszalka, G., Purine nucleoside synthesis, an efficient method employing nucleoside phosphorylases (1981) Biochemistry, 20, pp. 3615-3621 
504 |a Krenitsky, T., Koszalka, G., Tuttle, J., Rideout, J., Elion, G., An enzymatic synthesis of purine D-arabinonucleosides (1981) Carbohydrate Res., 97, pp. 139-146 
504 |a Ling, F., Inoue, Y., Kimura, A., Purification and characterization of a novel nucleoside phosphorylase from a Klebsiella sp. and its use in the enzymatic production of adenine arabinoside (1990) Appl. Environ. Microbiol., 56, pp. 3830-3834 
504 |a Murakami, K., Shirasaka, T., Yoshioka, H., Kojima, E., Aoki, S., Ford, H., Driscoll, J., Mitsuya, H., Escherichia coli mediated biosynthesis and in vitro anti-HIV activity of lipophilic 6-halo-2',3'-dideoxypurine nucleosides (1991) J. Med. Chem., 34, pp. 1606-1612 
504 |a Pal, S., Nair, V., Enzymatic synthesis of thymidine using bacterial whole cells and isolated purine nucleoside phosphorylase (1997) Biotechnol. Lett., 19, pp. 147-158 
504 |a Perigaud, C., Gosselin, G., Imbach, J.L., Nucleosides analogues as chemoterapeutic agents: A review (1992) Nucleosides Nucleotides, 11, pp. 903-945 
504 |a Utagawa, T., Enzymatic preparation of nucleoside antibiotics (1999) J. Mol. Catal. B: Enzymatic, 6, pp. 215-222 
504 |a Utagawa, T., Morisawa, H., Yoshinaga, F., Yamazaki, A., Mitsugi, K., Hirose, Y., Microbiological synthesis of adenine arabinoside (1985) Agric. Biol. Chem., 49, pp. 1053-1058 
504 |a Yokozeki, K., Shirae, H., Kobayashi, K., Shiragami, H., Irie, Y., (1990), Method of Producing 2',3'-dideoxyinosine. US Patent No. 4,970,148 
520 3 |a E. coli BL21 synthesized purine nucleosides from pyrimidine ones. A 94% yield of adenosine from uridine was reached within 1 h.  |l eng 
536 |a Detalles de la financiación: Universidad Nacional de Quilmes 
536 |a Detalles de la financiación: Secretaría de Ciencia y Técnica, Universidad de Buenos Aires 
536 |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas 
536 |a Detalles de la financiación: We thank Universidad Nacional de Quilmes, Consejo Nacional de Investigaciones Científicas y Técnicas, Secretaría de Ciencia y Técnica de la Nación, from Argentine and International Centre for Genetic Engineering and Biology, Trieste, Italy for financial support. We are also grateful to Dr J.V. Sinisterra Gago for his fruitful advices. AMI and SP are research members of CONICET. 
593 |a Universidad Nacional de Quilmes, R. S. Peña 180, (1876) Bernal, Buenos Aires, Argentina 
593 |a INGEBI, CONICET, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina 
690 1 0 |a BIOTRANSFORMATIONS 
690 1 0 |a ESCHERICHIA COLI 
690 1 0 |a NUCLEOSIDE PHOSPHORYLASES 
690 1 0 |a PURINE NUCLEOSIDES 
690 1 0 |a NUCLEOSIDE DERIVATIVE 
690 1 0 |a ANTINEOPLASTIC ACTIVITY 
690 1 0 |a ANTIVIRAL ACTIVITY 
690 1 0 |a ARTICLE 
690 1 0 |a BIOTRANSFORMATION 
690 1 0 |a ESCHERICHIA COLI 
690 1 0 |a NONHUMAN 
690 1 0 |a PURINE SYNTHESIS 
690 1 0 |a ESCHERICHIA COLI 
690 1 0 |a NEGIBACTERIA 
700 1 |a Martínez, N. 
700 1 |a Rogert, M.C. 
700 1 |a Porro, S. 
700 1 |a Iribarren, A.M. 
773 0 |d 2000  |g v. 22  |h pp. 1277-1280  |k n. 16  |p Biotechnol. Lett.  |x 01415492  |w (AR-BaUEN)CENRE-148  |t Biotechnology Letters 
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856 4 0 |u https://doi.org/10.1023/A:1005689116502  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_01415492_v22_n16_p1277_Lewkowicz  |y Handle 
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