Early diagnosis of recurrence of Trypanosoma cruzi infection by polymerase chain reaction after heart transplantation of a chronic chagas' heart disease patient

Heart transplantation is contraindicated as an effective treatment for end-stage Chagas' heart disease because of post-operative recurrence of Trypanosoma cruzi infection and reactivation of disease after immunosupression. In a follow-up study of a heart transplanted patient with Chagas' d...

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Autor principal: Schijman, A.G
Otros Autores: Vigliano, C., Burgos, J., Favaloro, R., Perrone, S., Laguens, R., Levin, M.J
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2000
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
LEADER 06952caa a22009257a 4500
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005 20230518205216.0
008 190411s2000 xx ||||fo|||| 00| 0 eng|d
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030 |a JHLTE 
100 1 |a Schijman, A.G. 
245 1 0 |a Early diagnosis of recurrence of Trypanosoma cruzi infection by polymerase chain reaction after heart transplantation of a chronic chagas' heart disease patient 
260 |c 2000 
270 1 0 |m Levin, M.J.; Inst. Invest. en Ingeniera Genetica, Biologa Molecular, Vuelta de Obligado 2490, Buenos Aires 1428, Argentina; email: mlevin@dna.uba.ar 
506 |2 openaire  |e Política editorial 
504 |a Elizari, M.V., Chagasic myocardiopathy, historical perspective (1999) Medicina (B Aires), 59 (SUPPL. 2), pp. 25-40 
504 |a De Carvalho, V.B., Sousa, E.F., Vila, J.H., Heart transplantation in Chagas' disease. 10 Years after the initial experience (1996) Circulation, 94 (8), pp. 1815-1817 
504 |a Bocchi, E.A., Bellotti, G., Mocelin, A.O., Heart transplantation for chronic Chagas' heart disease (1996) Ann Thorac Surg, 61 (6), pp. 1727-1733 
504 |a Riarte, A., Luna, C., Sabatiello, R., Chagas' disease in patients with kidney transplants: 7 Years of experience. 1989-1996 (1999) Clin Infect Dis, 29 (3), pp. 561-567 
504 |a Billingham, M.E., Cary, N.R.B., Hammond, M.E., A working formulation for the standardization of nomenclature in the diagnosis of heart and lung rejection. Heart Rejection Study Group (1990) J Heart Transplant, 9, pp. 587-593 
504 |a Avila, H.A., Sigman, D.S., Cohen, L.M., Millikan, R.C., Simpson, L., Polymerase chain reaction amplification of Trypanosoma cruzi kinetoplast minicircle DNA isolated from whole blood lysates: Diagnosis of chronic Chagas' disease (1991) Mol Biochem Parasitol, 48 (2), pp. 211-221 
504 |a Brandariz, S., Schijman, A., Vigliano, C., Detection of Trypanosoma cruzi DNA from archival heart tissue of a chronic Chagas' disease reference case (1995) Lancet, 346, pp. 1370-1371 
504 |a Gonzalez, A., Prediger, E., Huecas, M.E., Nogueira, N., Lizard, P.M., Minichromosomal repetitive DNA in trypanosoma cruzi. Its use in a high-sensitivity parasite detection assay (1984) Proc Natl Acad Sci USA, 81, pp. 3356-3360 
520 3 |a Heart transplantation is contraindicated as an effective treatment for end-stage Chagas' heart disease because of post-operative recurrence of Trypanosoma cruzi infection and reactivation of disease after immunosupression. In a follow-up study of a heart transplanted patient with Chagas' disease, we prospectively evaluated the usefulness of the polymerase chain reaction (PCR) for early diagnosis of reactivation. We monitored post-operative recurrence of Trypanosoma cruzi infection with microscopic observation of the parasite in peripheral blood (Strout's method), endomyocardial biopsies (EMBs), skin lesions, and 2 PCR assays, based on the amplification of specific T cruzi kinetoplastid and nuclear DNA sequences. During follow-up, parasite DNA was amplified in blood samples and EMB sections 41 days before we observed patent parasitemia and cutaneous manifestations of reactivation, proving that PCR is much more sensitive than direct microscopic observation for early diagnosis of disease reactivation in heart-transplanted Chagas' disease patients.  |l eng 
536 |a Detalles de la financiación: Universidad de Buenos Aires, UBA 
536 |a Detalles de la financiación: John Simon Guggenheim Memorial Foundation 
536 |a Detalles de la financiación: PEI 107-97 
536 |a Detalles de la financiación: Fondo para la Investigación Científica y Tecnológica, FonCyT, TW 18 (1998-2000 
536 |a Detalles de la financiación: Fondo para la Investigación Científica y Tecnológica, FonCyT, PICT 02030 
536 |a Detalles de la financiación: Fondo para la Investigación Científica y Tecnológica, FonCyT, BID 802-OC-AR 
536 |a Detalles de la financiación: International Atomic Energy Agency, IAEA, RLA 6/026 
536 |a Detalles de la financiación: This work was supported in part by Roemmers Foundation, CONICET PEI 107-97, FONCYT Project BID 802-OC-AR, PICT 02030; the Research Project TW 18 (1998-2000) of the University of Buenos Aires; and by WHO/TDR and the International Atomic Energy Agency, through the Latin American Technical Research Project RLA 6/026. Mariano Jorge Levine is fellow of the John Simon Guggenheim Memorial Foundation. This article is dedicated to the memory of Dr. René Favaloro. 
593 |a Instituto De Investigaciones En Ingeniería Genética Y Biología Molecular, Buenos Aires, Argentina 
593 |a Instituto De Cardiología Y Cirugía Cardiovascular, Fundación Favaloro, Buenos Aires, Argentina 
593 |a Instituto De Investigaciones En Ingeniería Genética Y Biología Molecular, Vuelta de Obligado 2490, Buenos Aires, 1428, Argentina 
690 1 0 |a ARTICLE 
690 1 0 |a CASE REPORT 
690 1 0 |a CHAGAS DISEASE 
690 1 0 |a HEART MUSCLE BIOPSY 
690 1 0 |a HEART TRANSPLANTATION 
690 1 0 |a HUMAN 
690 1 0 |a HUMAN TISSUE 
690 1 0 |a POLYMERASE CHAIN REACTION 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a RECURRENT INFECTION 
690 1 0 |a TRYPANOSOMA CRUZI 
690 1 0 |a TRYPANOSOMIASIS 
690 1 0 |a ANIMALS 
690 1 0 |a BIOPSY 
690 1 0 |a CHAGAS CARDIOMYOPATHY 
690 1 0 |a ENDOCARDIUM 
690 1 0 |a FEMALE 
690 1 0 |a FOLLOW-UP STUDIES 
690 1 0 |a GRAFT REJECTION 
690 1 0 |a HEART TRANSPLANTATION 
690 1 0 |a HUMANS 
690 1 0 |a MIDDLE AGED 
690 1 0 |a MYOCARDIUM 
690 1 0 |a POLYMERASE CHAIN REACTION 
690 1 0 |a POSTOPERATIVE COMPLICATIONS 
690 1 0 |a PREDICTIVE VALUE OF TESTS 
690 1 0 |a PROSPECTIVE STUDIES 
690 1 0 |a RECURRENCE 
690 1 0 |a TRYPANOSOMA CRUZI 
700 1 |a Vigliano, C. 
700 1 |a Burgos, J. 
700 1 |a Favaloro, R. 
700 1 |a Perrone, S. 
700 1 |a Laguens, R. 
700 1 |a Levin, M.J. 
773 0 |d 2000  |g v. 19  |h pp. 1114-1117  |k n. 11  |p J. Heart Lung Transplant.  |x 10532498  |t Journal of Heart and Lung Transplantation 
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856 4 0 |u https://doi.org/10.1016/S1053-2498(00)00168-6  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_10532498_v19_n11_p1114_Schijman  |y Handle 
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