How does hexachlorobenzene treatment affect liver uroporphyrinogen decarboxylase?
The aims of the present work were: (1) to investigate whether the strong decrease of liver uroporphyrinogen decarboxylase (UroD) activity observed in experimental porphyria cutanea tarda is due to alteration of the enzymatic protein and (2) to improve the knowledge about the normal liver enzyme. Wit...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
2001
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 13139caa a22013097a 4500 | ||
|---|---|---|---|
| 001 | PAPER-20627 | ||
| 003 | AR-BaUEN | ||
| 005 | 20240904173001.0 | ||
| 008 | 190411s2001 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0034990683 | |
| 024 | 7 | |2 cas |a Antigens; Fungicides, Industrial; Hexachlorobenzene, 118-74-1; Uroporphyrinogen Decarboxylase, EC 4.1.1.37 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a IJBBF | ||
| 100 | 1 | |a Chaufan, G. | |
| 245 | 1 | 4 | |a How does hexachlorobenzene treatment affect liver uroporphyrinogen decarboxylase? |
| 260 | |c 2001 | ||
| 270 | 1 | 0 | |m San Martín de Viale, L.C.; Departamento de Quimica Biologica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina; email: smartin@qb.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Mauzerall, D., Granick, S., Porphyrin biosynthesis in erythrocytes. Uroporphyrinogen and its decarboxylase (1958) J. Biol. Chem., 232, pp. 1141-1162 | ||
| 504 | |a San Martín de Viale, L.C., Ríos de Molina, M.C., Wainstok de Calmanovici, R., Tomio, J.M., Porphyrins and porphyrinogen carboxylyase in hexachlorobenzene-induced porphyria (1977) Biochem. J., 168, pp. 393-400 | ||
| 504 | |a De Salamanca, R.E., Batlle, A.M.D.C., Chinarro, S., Stella, A.M., Wider, E.A., Estudio comparativo de la Porfiria Experimental inducida por hexaclorobenceno y la Porfiria cutánea Tardía Humana (1985) An. Med. Int., 2, pp. 319-329 | ||
| 504 | |a Elder, G.H., Porphyria cutanea tarda (1998) Semin. Liver Dis., 18, pp. 67-75 | ||
| 504 | |a García, R.C., San Martín de Viale, L.C., Tomio, M.J., Grinstein, M., Porphyrin biosynthesis. Porphyrinogen carboxy-lyase from avian erythrocytes. Further properties (1973) Biochem. Biophys. Acta, 209, pp. 203-210 | ||
| 504 | |a Tomio, J.M., García, R.C., San Martín de Viale, L.C., Grinstein, M., Porphyrin biosynthesis. Porphyrinogen carboxy-lyase from avian erythrocytes. Purification and properties (1970) Biochem. Biophys. Acta, 198, pp. 353-363 | ||
| 504 | |a De Verneuil, H., Grandchamp, B., Nordmann, Y., Some kinetic properties of human red cell uroporphyrinogen decarboxylase (1980) Biochem. Biophys. Acta, 611, pp. 174-186 | ||
| 504 | |a Straka, J.G., Kushner, J.P., Purification and characterization of bovine hepatic uroporphyrinogen decarboxylase (1983) Biochemistry, 22, pp. 4664-4672 | ||
| 504 | |a Ríos de Molina, M.C., Billi, S.C., San Martín de Viale, L.M., Does feeding of hexachlorobenzene promote structural changes in rat-liver porphyrinogen carboxy-lyase? (1986) Hexachlorobenzene Proceeding of an International Symposium, 77, pp. 481-486. , IARC Scientific Publications | ||
| 504 | |a Billi de Catabbi, S., Ríos de Molina, M.C., San Martín de Viale, L.C., Studies on the active center of rat liver porphyrinogen carboxy-lyase in-vivo effect of hexachlorobenzene (1991) Int. J. Biochem., 23, pp. 675-679 | ||
| 504 | |a Kawanishi, S., Seki, Y., Sano, S., Uroporphyrinogen decarboxylase. Purification, properties and inhibition by polychlorinated biphenyl isomers (1983) J. Biol. Chem., 258, pp. 4285-4292 | ||
| 504 | |a Elder, G.H., Tovey, J.A., Sheppard, D.M., Purification of uroporphyrinogen decarboxylase from human erythrocytes (1983) Biochem. J., 215, pp. 46-55 | ||
| 504 | |a Koopman, G.E., Juknat de Geralnik, A.A., Batlle, A.M.D.C., Porphyrin biosynthesis in Rhodopseudomonas palustris - V. Purification of porphyrinogen decarboxylase and some unusual properties (1986) Int. J. Biochem., 18, pp. 935-944 | ||
| 504 | |a Wyckoff, E.E., Phillips, D.J., Sowa, A.M., Franklin, M.R., Kushner, J.P., Mutational analysis of human uroporphyrinogen decarboxylase (1996) Biochem. Biophys. Acta, 1298, pp. 194-204 | ||
| 504 | |a Franklin, M.R., Phillips, J.D., Kushner, J.P., Cytochrome P450 induction, uroporphyrinogen decarboxylase depression, porphyrins accumulation and excretion, and gender influence in a 3-week rat model of Porphyria Cutanea Tarda (1997) Toxicol. Appl. Pharmacol., 147, pp. 289-299 | ||
| 504 | |a Ríos de Molina, M.C., Wainstok de Calmanovici, R., San Martín de Viale, L.C., Investigation of the presence of porphyrinogen carboxy-lyase inhibitor in liver rats intoxicated with hexachlorobenzene (1980) Int. J. Biochem., 12, pp. 1027-1032 | ||
| 504 | |a Cantoni, L., Dal Fiume, D., Ruggieri, R., Decarboxylation of uroporphyrinogen I and III in 2,3,7,8-tetrachlorodibenzo-p-dioxin induced porphyria in mice (1984) Int. J. Biochem., 16, pp. 561-565 | ||
| 504 | |a Smith, A.G., Francis, J.E., Chemically induced formation of an inhibitor of hepatic uroporphyrinogen decarboxylase in inbred mice with an iron overload (1987) Biochem. J., 246, pp. 221-226 | ||
| 504 | |a Francis, J.E., Smith, A.G., Oxidation of uroporphyrinogen by hydroxyl radicals. Evidence of non porphyrin products as potential inhibitors of uroporphyrinogen decarboxylase (1988) FEBS Lett., 233, pp. 311-314 | ||
| 504 | |a De Mateis, F., Harvey, C., Reed, C., Hempenius, R., Increased oxidation of uroporphyrinogen by an inducible liver microsomal system (1988) Biochem. J., 250, pp. 161-169 | ||
| 504 | |a Lambrecht, R.W., Jacobs, J.M., Sinclair, P.R., Sinclair, J.F., Inhibition of uroporphyrinogen decarboxylase activity. The role of cytochrome P-450 mediated uroporphyrinogen oxidation (1990) Biochem. J., 269, pp. 437-441 | ||
| 504 | |a Billi de Catabbi, S., Wainstok de Calmanovici, R., Minutolo, C., Aldonatti, C., San Martín de Viale, L.C., Porphyria induced hepatic porphyrinogen carboxy-lyase inhibitor and its interaction with the active site (s) of the enzyme (1999) Biochem. Mol. Biol. Int., 47, pp. 945-956 | ||
| 504 | |a Hindmarsh, J.T., Enzyme heterogeneity in the porphyrias (1990) Clin. Biochem., 23, pp. 371-374 | ||
| 504 | |a Mylchreest, E., Charbonneau, M., Studies on the mechanism of uroporphyrinogen decarboxylase inhibition in hexachlorobenzene-induced porphyria in the female rat (1997) Toxicol. Appl. Pharmacol., 145, pp. 23-33 | ||
| 504 | |a De Verneuil, H., Sassa, S., Kappas, A., Purification and properties of uroporphyrinogen decarboxylase from human erythrocytes. A single enzyme catalyzing the four sequential decarboxylations of uroporphyrinogen I and III (1983) J. Biol. Chem., 258, pp. 2454-2460 | ||
| 504 | |a Mukerji, S.K., Pimstone, N.R., Uroporphyrinogen decarboxylase from human erythrocytes: Purification, complete separation and partial characterization of two isoenzymes (1992) Int. J. Biochem., 24, pp. 105-119 | ||
| 504 | |a Phillips, J.D., Whitby, F.G., Kushner, J.P., Hill, C.P., Characterization and crystallization of human uroporphyrinogen decarboxylase (1997) Prot. Sci., 6, pp. 1343-1346 | ||
| 504 | |a Ríos de Molina, M.C., Chaufan, G., Iglesias, S., Billi de Catabbi, S., San Martín de Viale, L.C., Porphyrinogen carboxylase. Studies on the existence of isoenzymes (1996) Acta Physiol. Pharmacol. Ther. Latinoam., 46, pp. 265-275 | ||
| 504 | |a Bradford, M.M., A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal. Biochem., 72, pp. 248-254 | ||
| 504 | |a Billi de Catabbi, S., San Martín de Viale, L.C., Studies on the active center(s) of rat liver porphyrinogen carboxy-lyase. In-vivo effect of hexachlorobenzene on decarboxylation site(s) of porphyrinogens (1994) Int. J. Biochem., 26, pp. 595-600 | ||
| 504 | |a Elder, G.H., Sheppard, D.M., Immunoreactive uroporphyrinogen decarboxylase is unchanged in porphyria caused by TCDD and hexachlorobenzene (1982) Biochem. Biophys. Res. Commun., 109, pp. 113-120 | ||
| 520 | 3 | |a The aims of the present work were: (1) to investigate whether the strong decrease of liver uroporphyrinogen decarboxylase (UroD) activity observed in experimental porphyria cutanea tarda is due to alteration of the enzymatic protein and (2) to improve the knowledge about the normal liver enzyme. With these purposes, several physicochemical studies for enzymatic characterization were carried out comparatively on the 12-fold purified liver enzyme of both normal and hexachlorobenzene porphyric rat. The study shows that the enzyme from porphyric rats has a higher activation energy, lower reactivity index and lower optimum pH than the normal one. In addition, it did not reach the Vmax at any of the substrate concentrations assayed (up to 28 μM uroporphyrinogen III), while the normal enzyme reached the plateau around 14 μM. The porphyric enzyme appears to be more protected than the normal against the inhibitory action of several metals, particularly Cu2+ and Pb2+, and against thermal inactivation. Zn2+ did not affect enzymatic activity, whereas Cu2+, Hg2+, Fe2+, Pb2+, and Cd2+ lowered the activities of both normal and porphyric enzyme in a dose-related way. It was also observed that the larger the atomic radius in its hydrated state, the lower the effect of the metal. Neither glutathione nor dithiothreitol significantly altered enzymatic activity in the range of concentrations assayed. β-Mercaptoethanol had diverse effects, as regards both the concentration assayed and the enzymatic sample used. Assays with cystine showed a dual behaviour of both normal and porphyric enzymatic activity. Western blots for both preparations revealed a single band (65 kDa) with a similar intensity. This study show that hexachlorobenzene treatment modifies the physicochemical properties of liver UroD leading to a sharp decrease of its activity, without affecting its antigenic reactivity probably as a consequence of changes at the conformational level promoted by the binding of its reported inhibitor. © 2001 Elsevier Science Ltd. |l eng | |
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: This work was partially supported by grants from the Consejo Nacional de Investigaciones Cientı́ficas y Técnicas (CONICET) and from the University of Buenos Aires. We thank Mr. G. Taminelli for his collaboration in some of the experiments, and Mrs. C. Aldonatti (Technical Assistance Career member of the CONICET) for her technical assistance. G. Chaufan has a fellowship from the CONICET, and L.C. San Martı́n de Viale and M.C. Rı́os de Molina are Scientific Research Career members of the CONICET. | ||
| 593 | |a Laboratorio De Porfirias Experimentales Y Metabolismo del Hemo, Departamento De Química Biológica, Universidad De Buenos Aires, Buenos Aires, Argentina | ||
| 593 | |a O'Higgins 4332, Capital, C.P. 1429, Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a ACTIVE SITE |
| 690 | 1 | 0 | |a ANTIGENIC DETERMINANTS |
| 690 | 1 | 0 | |a HEXACHLOROBENZENE |
| 690 | 1 | 0 | |a PORPHYRIA CUTANEA TARDA |
| 690 | 1 | 0 | |a UROPORPHYRINOGEN DECARBOXYLASE |
| 690 | 1 | 0 | |a CADMIUM |
| 690 | 1 | 0 | |a COPPER ION |
| 690 | 1 | 0 | |a CYSTINE |
| 690 | 1 | 0 | |a DITHIOTHREITOL |
| 690 | 1 | 0 | |a GLUTATHIONE |
| 690 | 1 | 0 | |a HEXACHLOROBENZENE |
| 690 | 1 | 0 | |a LEAD |
| 690 | 1 | 0 | |a LIVER ENZYME |
| 690 | 1 | 0 | |a MERCAPTOETHANOL |
| 690 | 1 | 0 | |a MERCURY |
| 690 | 1 | 0 | |a UROPORPHYRINOGEN DECARBOXYLASE |
| 690 | 1 | 0 | |a ZINC ION |
| 690 | 1 | 0 | |a ANIMAL EXPERIMENT |
| 690 | 1 | 0 | |a ANIMAL MODEL |
| 690 | 1 | 0 | |a ANTIGENICITY |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a ATOM |
| 690 | 1 | 0 | |a CONCENTRATION RESPONSE |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a DRUG ACTIVITY |
| 690 | 1 | 0 | |a ENZYME ACTIVITY |
| 690 | 1 | 0 | |a ENZYME ANALYSIS |
| 690 | 1 | 0 | |a ENZYME BINDING |
| 690 | 1 | 0 | |a ENZYME CONFORMATION |
| 690 | 1 | 0 | |a ENZYME INHIBITION |
| 690 | 1 | 0 | |a HYDRATION |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PHYSICAL CHEMISTRY |
| 690 | 1 | 0 | |a PORPHYRIA CUTANEA TARDA |
| 690 | 1 | 0 | |a RAT |
| 690 | 1 | 0 | |a ANIMALS |
| 690 | 1 | 0 | |a ANTIGENS |
| 690 | 1 | 0 | |a FEMALE |
| 690 | 1 | 0 | |a FUNGICIDES, INDUSTRIAL |
| 690 | 1 | 0 | |a HEXACHLOROBENZENE |
| 690 | 1 | 0 | |a HYDROGEN-ION CONCENTRATION |
| 690 | 1 | 0 | |a LIVER |
| 690 | 1 | 0 | |a RATS |
| 690 | 1 | 0 | |a RATS, WISTAR |
| 690 | 1 | 0 | |a TEMPERATURE |
| 690 | 1 | 0 | |a UROPORPHYRINOGEN DECARBOXYLASE |
| 650 | 1 | 7 | |2 spines |a PH |
| 700 | 1 | |a Ríos de Molina, María del Carmen | |
| 700 | 1 | |a San Martín de Viale, L.C. | |
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