Different functions of two alarm substances in the honeybee

In the honeybee, isopentyl acetate and 2-heptanone are described as alarm substances. We asked whether both substances have a similar role by testing the effect of their exposure on the appetitive proboscis extension reflex and on the aversive stinging reflex. In the appetitive context of sucrose st...

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Autor principal: Balderrama, N.
Otros Autores: Núñez, J., Guerrieri, F., Giurfa, M.
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2002
Acceso en línea:Registro en Scopus
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024 7 |2 scopus  |a 2-s2.0-0036667084 
024 7 |2 cas  |a 2 heptanone, 110-43-0; 2-heptanone, 110-43-0; Acetates; Ketones; Pheromones 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a JCPAD 
100 1 |a Balderrama, N. 
245 1 0 |a Different functions of two alarm substances in the honeybee 
260 |c 2002 
270 1 0 |m Giurfa, M.; Laboratoire de Cognition Animale, Université Paul-Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex 4, France; email: giurfa@cict.fr 
506 |2 openaire  |e Política editorial 
504 |a Boch, R., Rothenbühler, W.C., Defensive behaviour and production of alarm pheromone in honeybees (1974) J Apic Res, 13, pp. 217-221 
504 |a Boch, R., Shearer, D.A., 2-Heptanone, 10-hydroxy-transdec-2-enoic acid in the mandibular glands of worker honeybees of different ages (1967) Z Vergl Physiol, 54, pp. 1-11 
504 |a Boch, R., Shearer, D.A., Chemical releasers of alarm behaviour in the honey bee (1971) J Insect Physiol, 17, pp. 2277-2285 
504 |a Boch, R., Shearer, D.A., Stone, B.C., Identification of iso-amyl acetate as an active component in the sting pheromone of the honeybees (1962) Nature, 195, pp. 1018-1020 
504 |a Boch, R., Shearer, D.A., Petrasovits, A., Efficacies of two alarm substances of the honey bee (1970) J Insect Physiol, 16, pp. 17-24 
504 |a Butler, C.G., Mandibular gland pheromone of worker honey bee (1966) Nature, 212, p. 530 
504 |a Crewe, R.M., Hastings, H., Production of pheromones by workers of Apis mellifera adansonii (1976) J Apic Res, 15, pp. 149-154 
504 |a Fanselow, M.S., Pavlovian conditioning, negative feedback, and blocking: Mechanisms that regulate association formation (1998) Neuron, 20, pp. 625-627 
504 |a Free, J.B., (1987) Pheromones of Social Bees, , Comstock, New York 
504 |a Free, J.B., Simpson, J., The alerting pheromones of the honeybee (1968) Z Vergl Physiol, 61, pp. 361-365 
504 |a Giurfa, M., The repellent scent mark of the honeybee Apis mellifera ligustica and its role as communication cue during foraging (1993) Insect Soc, 40, pp. 59-67 
504 |a Giurfa, M., Núñez, J.A., Honeybees mark with scent and reject recently visited flowers (1992) Oecologia, 89, pp. 113-117 
504 |a Giurfa, M., Núñez, J.A., Visual modulation of a scent marking activity in the honeybee Apis mellifera L. (1993) Naturwissenschaften, 80, pp. 376-379 
504 |a Kuwabara, M., Bildung des bedingten reflexes von pavlovs typus bei der honigbiene, Apis mellifica (1957) J Fac Sci Hokkaido Univ VI Zool, 13, pp. 458-464 
504 |a Maschwitz, U.W., Gefahrenalarmstoffe und gefahrenalarmierung bei sozialen hymenopteren (1964) Z Vergl Physiol, 47, pp. 596-655 
504 |a Núñez, J.A., Sammelbienen markieren versiegte futterquellen durch duft (1967) Naturwissenschaften, 54, pp. 322-323 
504 |a Núñez, J.A., Almeida, L., Balderrama, N., Giurfa, M., Alarm pheromone induces stress analgesia via an opioid system in the honeybee (1998) Physiol Behav, 63, pp. 75-80 
504 |a Shearer, D.A., Boch, R., 2-Heptanone in the mandibular gland secretion of the honeybee (1965) Nature, 206, p. 530 
504 |a Simpson, J., The salivary glands of Apis mellifera and their significance in caste determination (1961) Atti V Congresso UIEIS, 10, pp. 173-188. , Pavia 
504 |a Simpson, J., Repellency of the mandibular gland scent of worker honey bees Nature, 203, pp. 531-532 
504 |a Vallet, A., Cassier, P., Lensky, Y., Ontogeny of the fine structure of the mandibular glands of the honeybee (Apis mellifera L.) workers and the pheromonal activity of 2-heptanone (1991) J Insect Physiol, 37, pp. 789-804 
504 |a Zar, J.H., (1984) Biostatistical Analysis, 2nd Edn., , Prentice Hall, New Jersey 
520 3 |a In the honeybee, isopentyl acetate and 2-heptanone are described as alarm substances. We asked whether both substances have a similar role by testing the effect of their exposure on the appetitive proboscis extension reflex and on the aversive stinging reflex. In the appetitive context of sucrose stimulation no differences were found between isopentyl acetate and 2-heptanone. Small amounts of isopentyl acetate or 2-heptanone (3 μl of 1:9 dilution) yielded a response similar to that of a non-exposed control. Larger amounts of both substances (125 μl of 1:9 dilutions) as well as mixtures led to a decrease of responsiveness to sucrose. In the aversive context of electrical stimulation, significant differences between isopentyl acetate and 2-heptanone were found. Exposure to a small amount of isopentyl acetate (3 μl of 1:9 dilution) or to a large amount of 2-heptanone (125 μl of 1:9 dilution) led to an increase of responsiveness to the electric shock. Larger quantities of isopentyl acetate (125 μl of 1:9 dilution) decreased the responsiveness to the shock. 2-Heptanone never decreased the responsiveness to the shock. Our results indicate that isopentyl acetate and 2-heptanone have different functions even if both are capable of evoking deterrent responses in a defensive context.  |l eng 
536 |a Detalles de la financiación: Consejo de Desarrollo Científico y Humanístico, Universidad Central de Venezuela 
536 |a Detalles de la financiación: Alexander von Humboldt-Stiftung 
536 |a Detalles de la financiación: Universidad Central de Venezuela, 01.38.3313/94 
536 |a Detalles de la financiación: Université Toulouse III - Paul Sabatier 
536 |a Detalles de la financiación: Acknowledgements The authors thank J.C. Sandoz, C. Reiseman, and two anonymous referees for comments and suggestions on the manuscript. N. Balderrama thanks the Consejo de Desarrollo Científico y Humanístico (CDCH), UCV, Venezuela (grant 01.38.3313/94), J.A. Núñez the Alexander von Humboldt-Stiftung, and F. Guerrieri and M. Giurfa the Procope Funding Program and funding ASUPS from the Université Paul Sabatier. F. Guerrieri was supported by a José A. Estenssoro fellowship of the Fundación YPF-Argentina. The experiments comply with the Principles of Health, publication No. 86-23, revised 1985 of the National Institute of Health and the current laws of Venezuela, where the experiments were performed. 
593 |a Inst. de Zool. Agrícola, Facultad de Agronomia, Universidad Central de Venezuela, Aptdo. Postal 4579, Maracay 2101, Aragua, Venezuela 
593 |a Laboratory of Insect Physiology, Department of Biological Sciences, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina 
593 |a Laboratoire de Cognition Animale, Université Paul-Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex 4, France 
690 1 0 |a 2-HEPTANONE 
690 1 0 |a HONEYBEE 
690 1 0 |a ISOPENTYL ACETATE 
690 1 0 |a PROBOSCIS EXTENSION REFLEX 
690 1 0 |a STINGING REFLEX 
690 1 0 |a 2 HEPTANONE 
690 1 0 |a 2-HEPTANONE 
690 1 0 |a ACETIC ACID DERIVATIVE 
690 1 0 |a KETONE 
690 1 0 |a PHEROMONE 
690 1 0 |a AGGRESSION 
690 1 0 |a ANIMAL 
690 1 0 |a APPETITE 
690 1 0 |a ARTICLE 
690 1 0 |a BEE 
690 1 0 |a CONDITIONING 
690 1 0 |a DRUG EFFECT 
690 1 0 |a MOUTH 
690 1 0 |a PHYSIOLOGY 
690 1 0 |a REFLEX 
690 1 0 |a ACETATES 
690 1 0 |a AGGRESSION 
690 1 0 |a ANIMALS 
690 1 0 |a APPETITE 
690 1 0 |a BEES 
690 1 0 |a CONDITIONING (PSYCHOLOGY) 
690 1 0 |a KETONES 
690 1 0 |a MOUTH 
690 1 0 |a PHEROMONES 
690 1 0 |a REFLEX 
700 1 |a Núñez, J. 
700 1 |a Guerrieri, F. 
700 1 |a Giurfa, M. 
773 0 |d 2002  |g v. 188  |h pp. 485-491  |k n. 6  |p J. Comp. Physiol. A Neuroethol. Sens. Neural. Behav. Physiol.  |x 03407594  |w (AR-BaUEN)CENRE-233  |t Journal of Comparative Physiology A: Neuroethology, Sensory, Neural, and Behavioral Physiology 
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