Expression and structure-function analysis of DE, a sperm cysteine-rich secretory protein that mediates gamete fusion
Rat sperm epididymal glycoprotein DE belongs to the cysteine-rich secretory protein (CRISP) family and participates in sperm-egg fusion through its binding to complementary sites on the egg surface. To investigate the molecular mechanisms underlying the role of DE in gamete fusion, in the present wo...
Guardado en:
| Autor principal: | |
|---|---|
| Otros Autores: | , , , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
Society for the Study of Reproduction
2002
|
| Materias: | |
| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 15640caa a22014777a 4500 | ||
|---|---|---|---|
| 001 | PAPER-20288 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518205141.0 | ||
| 008 | 190411s2002 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0036785275 | |
| 024 | 7 | |2 cas |a biotin, 58-85-5; disulfide, 16734-12-6; dithiothreitol, 3483-12-3; maleimide, 541-59-3; thiol derivative, 13940-21-1 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a BIREB | ||
| 100 | 1 | |a Ellerman, D.A. | |
| 245 | 1 | 0 | |a Expression and structure-function analysis of DE, a sperm cysteine-rich secretory protein that mediates gamete fusion |
| 260 | |b Society for the Study of Reproduction |c 2002 | ||
| 270 | 1 | 0 | |m Cuasnicú, P.S.; Inst. de Biol./Medicina Experimental, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina; email: cuasnicu@dna.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Myles, D.G., Molecular mechanisms of sperm-egg membrane binding and fusion in mammals (1993) Dev Biol, 158, pp. 35-45 | ||
| 504 | |a Evans, J.P., Sperm disintegrins, egg integrins, and other cell adhesion molecules of mammalian gamete plasma membrane interactions (1999) Front Biosci, 4, pp. D114-D131 | ||
| 504 | |a Cameo, M.S., Blaquier, J.A., Androgen-controlled specific proteins in rat epididymis (1976) J Endocr, 69, pp. 317-324 | ||
| 504 | |a Garberi, J.C., Kohane, A.C., Cameo, M.S., Blaquier, J.A., Isolation and characterization of specific rat epididymal proteins (1979) Mol Cell Endocrinol, 13, pp. 73-82 | ||
| 504 | |a Garberi, J.C., Fontana, J.D., Blaquier, J.A., Carbohydrate composition of specific rat epididymal protein (1982) Int J Androl, 5, pp. 619-626 | ||
| 504 | |a Kohane, A.C., Cameo, M.S., Piñeiro, L., Garberi, J.C., Blaquier, J.A., Distribution and site of production of specific proteins in the rat epididymis (1980) Biol Reprod, 23, pp. 181-187 | ||
| 504 | |a Kohane, A.C., Gonzalez Echeverria, F., Piñeiro, L., Blaquier, J.A., Interaction of proteins of epididymal origin with spermatozoa (1980) Biol Reprod, 23, pp. 737-742 | ||
| 504 | |a Rochwerger, L., Cuasnicu, P.S., Redistribution of a rat sperm epididymal glycoprotein after in vivo and in vitro capacitation (1992) Mol Reprod Dev, 31, pp. 34-41 | ||
| 504 | |a Cohen, D.J., Rochwerger, L., Ellerman, D.A., Morgenfeld, M., Busso, D., Cuasnicu, P.S., Relationship between the association of rat epididymal protein DE with spermatozoa and the behavior and function of the protein (2000) Mol Reprod Dev, 56, pp. 180-188 | ||
| 504 | |a Cuasnicú, P.S., Conesa, D., Rochwerger, L., Potential contraceptive use of an epididymal protein that participates in fertilization (1990) Gamete Interaction. Prospects for Immunocontraception, pp. 143-153. , Alexander NJ, Griffin D, Spieler JM, Waites GMH (eds.). New York: Wiley-Liss | ||
| 504 | |a Rochwerger, L., Cohen, D.J., Cuasnicú, P.S., Mammalian sperm-egg fusion: The rat egg has complementary sites for a sperm protein that mediates gamete fusion (1992) Dev Biol, 153, pp. 83-90 | ||
| 504 | |a Cohen, D.J., Munuce, M.J., Cuasnicú, P.S., Mammalian sperm-egg fusion: The development of rat oolemma fusibility during oogenesis involves the appearance of binding sites for sperm protein "DE" (1996) Biol Reprod, 55, pp. 200-206 | ||
| 504 | |a Johnson, M.H., Eager, D., Muggleton-Harris, A., Grave, H.M., Mosaicism in organisation of concanavalin A receptors on surface membranes of mouse eggs (1975) Nature, 257, pp. 321-322 | ||
| 504 | |a Ebensperger, C., Barros, C., Changes at the hamster oocyte surface from germinal vesicle stage to ovulation (1984) Gamete Res, 9, pp. 387-397 | ||
| 504 | |a Mizuki, N., Kasahara, M., Mouse submandibular glands express an androgen-regulated transcript encoding an acidic epididymal glycoprotein-like molecule (1992) Mol Cell Endocrinol, 89, pp. 25-32 | ||
| 504 | |a Eberspaecher, U., Roosterman, D., Krätzschmar, J., Haendler, B., Habenicht, U.F., Becker, A., Quensel, C., Kratzschmar, J., Mouse androgen-dependent epididymal glycoprotein CRISP-1 (DE/AEG): Isolation, biochemical characterization, and expression in recombinant form (1995) Mol Reprod Dev, 42, pp. 157-172 | ||
| 504 | |a Hayashi, M., Fujimoto, S., Takano, H., Ushiki, T., Abe, K., Ishikura, H., Yoshida, M., Kasahara, M., Characterization of a human glycoprotein with potential role in sperm-egg fusion: cDNA cloning, immunohistochemical localization, and chromosomal assignment of the gene (AEGL1) (1996) Genomics, 32, pp. 367-374 | ||
| 504 | |a Kratzschmar, J., Haendler, B., Eberspaecher, U., Roosterman, D., Donner, P., Schleuning, W.D., The human cysteine-rich secretory protein (CRISP) family. Primary structure and tissue distribution of CRISP-1, CRISP-2 and CRISP-3 (1996) Eur J Biochem, 236, pp. 827-836 | ||
| 504 | |a Cohen, D.J., Ellerman, D.A., Cuasnicú, P.S., Mammalian sperm-egg fusion: Evidence that epididymal protein DE plays a role in mouse gamete fusion (2000) Biol Reprod, 63, pp. 462-468 | ||
| 504 | |a Cohen, D.J., Ellerman, D.A., Busso, D., Morgenfeld, M., Piazza, A., Hayashi, M., Young, E.T., Cuasnicu, P.S., Evidence that human epididymal protein ARP plays a role in gamete fusion through complementary sites on the surface of the human egg (2001) Biol Reprod, 65, pp. 1000-1005 | ||
| 504 | |a Kasahara, M., Gutknecht, J., Brew, K., Spurr, N., Goodfellow, P.N., Cloning and mapping of a testis-specific gene with sequence similarity to a sperm-coating glycoprotein gene (1989) Genomics, 5, pp. 527-534 | ||
| 504 | |a Foster, J.A., Gerton, G.L., Autoantigen 1 of the guinea pig sperm acrosome is the homologue of mouse Tpx-1 and human TPX1 and is a member of the cysteine-rich secretory protein (CRISP) family (1996) Mol Reprod Dev, 44, pp. 221-229 | ||
| 504 | |a Maeda, T., Sakashita, M., Ohba, Y., Nakanishi, Y., Molecular cloning of the rat Tpx-1 responsible for the interaction between spermatogenic and Sertoli cells (1998) Biochem Biophys Res Commun, 248, pp. 140-146 | ||
| 504 | |a Magdaleno, L., Gasset, M., Varea, J., Schambony, A.M., Urbanke, C., Raida, M., Topfer-Petersen, E., Calvete, J.J., Biochemical and conformational characterisation of HSP-3, a stallion seminal plasma protein of the cysteine-rich secretory protein (CRISP) family (1997) FEBS Lett, 420, pp. 179-185 | ||
| 504 | |a Schambony, A., Gentzel, M., Wolfes, H., Raida, M., Neumann, U., Topfer-Petersen, E., Equine CRISP-3: Primary structure and expression in the male genital tract (1998) Biochim Biophys Acta, 1387, pp. 206-216 | ||
| 504 | |a Haendler, B., Kratzschmar, J., Theuring, F., Schleuning, W.D., Transcripts for cysteine-rich secretory protein-1 (CRISP-1; DE/AEG) and the novel related CRISP-3 are expressed under androgen control in the mouse salivary gland (1993) Endocrinology, 133, pp. 192-198 | ||
| 504 | |a Pfisterer, P., Konig, H., Hess, J., Lipowsky, G., Haendler, B., Schleuning, W.D., Wirth, T., CRISP-3, a protein with homology to plant defense proteins, is expressed in mouse B cells under the control of Oct2 (1996) Mol Cell Biol, 16, pp. 6160-6168 | ||
| 504 | |a Kjeldsen, L., Cowland, J.B., Johnsen, A.H., Borregaard, N., SGP28, a novel matrix glycoprotein in specific granules of human neutrophils with similarity to a human testis-specific gene product and a rodent sperm-coating glycoprotein (1996) FEBS Lett, 380, pp. 246-250 | ||
| 504 | |a Mochca-Morales, J., Martin, B.M., Possani, L.D., Isolation and characterization of helothermine, a novel toxin from Heloderma horridum horridum (Mexican beaded lizard) venom (1990) Toxicon, 28, pp. 299-309 | ||
| 504 | |a Morrissette, J., Kratzschmar, J., Haendler, B., El-Hayek, R., Mochca-Morales, J., Martin, B.M., Jitandrakumar, R.P., Possani, L.D., Primary structure and properties of helothermine, a peptide that blocks ryanodine receptors (1995) Biophys J, 68, pp. 2280-2288 | ||
| 504 | |a Chang, T.Y., Mao, S.H., Guo, Y.W., Cloning and expression of a cysteinerich venom protein from Trimeresurus mucrosquamatus (Taiwan habu) (1997) Toxicon, 35, pp. 879-888 | ||
| 504 | |a Brooks, D.E., Means, A.R., Wright, E.J., Singh, S.P., Tiver, K.K., Molecular cloning of the cDNA for androgen-dependent sperm-coating glycoproteins secreted by the rat epididymis (1986) Eur J Biochem, 161, pp. 13-18 | ||
| 504 | |a Kaplan, R., Kraicer, P.F., Effect of elevated calcium concentration on fertilization of rat oocytes in vitro (1978) Gamete Res, 1, pp. 281-285 | ||
| 504 | |a Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685 | ||
| 504 | |a Towbin, H., Staehelin, T., Gordon, J., Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications (1979) Proc Natl Acad Sci U S A, 76, pp. 4350-4360 | ||
| 504 | |a Kohane, A.C., Piñeiro, L., Blaquier, J.A., Androgen-controlled synthesis of specific protein in the rat epididymis (1983) Endocrinology, 112, pp. 1590-1596 | ||
| 504 | |a Makrides, S.C., Strategies for achieving high-level expression of genes in Escherichia coli (1996) Microbiol Rev, 60, pp. 512-538 | ||
| 504 | |a Hannig, G., Makrides, S.C., Strategies for optimizing heterologous protein expression in Escherichia coli (1998) Trends Biotechnol, 16, pp. 54-60 | ||
| 504 | |a Georgiou, G., Valax, P., Expression of correctly folded proteins in Escherichia coli (1996) Curr Opin Biotechnol, 7, pp. 190-197 | ||
| 504 | |a Baneyx, F., Recombinant protein expression in Escherichia coli (1999) Curr Opin Biotechnol, 10, pp. 411-421 | ||
| 504 | |a Parodi, A.J., Role of N-oligosaccharide endoplasmic reticulum processing reactions in glycoprotein folding and degradation (2000) Biochem J, 348 (PART 1), pp. 1-13 | ||
| 504 | |a Marquardt, T., Helenius, A., Misfolding and aggregation of newly synthesized proteins in the endoplasmic reticulum (1992) J Cell Biol, 117, pp. 505-513 | ||
| 504 | |a Brooks, D.E., Influence of incubation conditions, tunicamycin and castration on incorporation of [3H]mannose and [3H]fucose into rat epididymal glycoproteins in vitro (1983) J Reprod Fertil, 67, pp. 97-105 | ||
| 504 | |a O'Bryan, M.K., Loveland, K.L., Herszfeld, D., McFarlane, J.R., Hearn, M.T., De Kretser, D.M., Identification of a rat testis-specific gene encoding a potential rat outer dense fibre protein (1998) Mol Reprod Dev, 50, pp. 313-322 | ||
| 504 | |a Bardwell, J.C., Building bridges: Disulphide bond formation in the cell (1994) Mol Microbiol, 14, pp. 199-205 | ||
| 504 | |a Yanagimachi, R., Huang, T.T.F., Fleming, A.D., Kosower, N.S., Nicolson, G.L., Dithiothreitol, a disulfide-reducing agent, inhibits capacitation, acrosome reaction, and interaction with eggs by guinea pig spermatozoa (1983) Gamete Res, 7, pp. 145-154 | ||
| 504 | |a Ikawa, M., Wada, I., Kominami, K., Watanabe, D., Toshimori, K., Nishimune, Y., Okabe, M., The putative chaperone calmegin is required for sperm fertility (1997) Nature, 387, pp. 607-611 | ||
| 504 | |a Perez Martinez, S., Conesa, D., Cuasnicd, P.S., Potential contraceptive use of epididymal proteins: Evidence for the participation of specific antibodies against rat epididymal protein DE in male and female fertility inhibition (1995) J Reprod Immunol, 29, pp. 31-45 | ||
| 504 | |a Ellerman, D.A., Brantua, V.S., Martinez, S.P., Cohen, D.J., Conesa, D., Cuasnicu, P.S., Potential contraceptive use of epididymal proteins: Immunization of male rats with epididymal protein DE inhibits sperm fusion ability (1998) Biol Reprod, 59, pp. 1029-1036 | ||
| 520 | 3 | |a Rat sperm epididymal glycoprotein DE belongs to the cysteine-rich secretory protein (CRISP) family and participates in sperm-egg fusion through its binding to complementary sites on the egg surface. To investigate the molecular mechanisms underlying the role of DE in gamete fusion, in the present work we expressed DE in a prokaryotic system, and examined the relevance of carbohydrates and disulfide bonds for the biological activity of the protein. Immunofluorescence and sperm-egg fusion assays carried out in the presence of recombinant DE (recDE) revealed that this protein exhibits the ability to bind to the DE-egg binding sites and to inhibit gamete fusion, as does native DE (nDE). Comparison of the proteins indicated, however, that the inhibitory ability of recDE was significantly lower than that of nDE. This difference would not be due to the lack of carbohydrates in the bacterially expressed protein because enzymatically deglycosylated nDE was as able as the untreated protein to inhibit gamete fusion. To examine whether disulfide bridges are involved in DE activity, the presence of sulfhydryls in nDE and recDE was evaluated by the biotin-maleimide technique. Results indicated that, unlike nDE, in which all cysteines are involved in disulfide bonds, recDE contains free thiol groups. Subsequent experiments showed that reduction of nDE with dithiothreitol significantly decreased the ability of the protein to inhibit gamete fusion. Together, these results indicate that whereas carbohydrates do not have a role in DE-mediated gamete fusion, disulfide bridges are required for full biological activity of the protein. To our knowledge, this is the first study reporting the relevance of structural components for the function of a CRISP member. |l eng | |
| 593 | |a Inst. de Biol./Medicina Experimental, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a EPIDIDYMIS |
| 690 | 1 | 0 | |a FERTILIZATION |
| 690 | 1 | 0 | |a OVUM |
| 690 | 1 | 0 | |a SPERM |
| 690 | 1 | 0 | |a BIOTIN |
| 690 | 1 | 0 | |a CARBOHYDRATE |
| 690 | 1 | 0 | |a CYSTEINE RICH SECRETORY PROTEIN |
| 690 | 1 | 0 | |a DISULFIDE |
| 690 | 1 | 0 | |a DITHIOTHREITOL |
| 690 | 1 | 0 | |a GLYCOPROTEIN DE |
| 690 | 1 | 0 | |a MALEIMIDE |
| 690 | 1 | 0 | |a RECOMBINANT PROTEIN |
| 690 | 1 | 0 | |a RECOMBINANT PROTEIN DE |
| 690 | 1 | 0 | |a SECRETORY PROTEIN |
| 690 | 1 | 0 | |a THIOL DERIVATIVE |
| 690 | 1 | 0 | |a UNCLASSIFIED DRUG |
| 690 | 1 | 0 | |a ANIMAL CELL |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a BINDING SITE |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a DEGLYCOSYLATION |
| 690 | 1 | 0 | |a DISULFIDE BOND |
| 690 | 1 | 0 | |a DRUG ACTIVITY |
| 690 | 1 | 0 | |a ENZYME GLYCOSYLATION |
| 690 | 1 | 0 | |a EPIDIDYMIS |
| 690 | 1 | 0 | |a FEMALE |
| 690 | 1 | 0 | |a FERTILITY |
| 690 | 1 | 0 | |a FERTILIZATION |
| 690 | 1 | 0 | |a GAMETE |
| 690 | 1 | 0 | |a IMMUNOFLUORESCENCE |
| 690 | 1 | 0 | |a INHIBITION KINETICS |
| 690 | 1 | 0 | |a MALE |
| 690 | 1 | 0 | |a MOLECULAR MECHANICS |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a PROKARYOTE |
| 690 | 1 | 0 | |a PROTEIN EXPRESSION |
| 690 | 1 | 0 | |a RAT |
| 690 | 1 | 0 | |a SPERM |
| 690 | 1 | 0 | |a STRUCTURE ACTIVITY RELATION |
| 690 | 1 | 0 | |a STRUCTURE ANALYSIS |
| 690 | 1 | 0 | |a ANIMALIA |
| 690 | 1 | 0 | |a PROKARYOTA |
| 650 | 1 | 7 | |2 spines |a FUSION |
| 700 | 1 | |a Da Ros, V.G. | |
| 700 | 1 | |a Cohen, D.J. | |
| 700 | 1 | |a Busso, D. | |
| 700 | 1 | |a Morgenfeld, M.M. | |
| 700 | 1 | |a Cuasnicú, P.S. | |
| 773 | 0 | |d Society for the Study of Reproduction, 2002 |g v. 67 |h pp. 1225-1231 |k n. 4 |p Biol. Reprod. |x 00063363 |t Biology of Reproduction | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036785275&doi=10.1095%2fbiolreprod67.4.1225&partnerID=40&md5=859fb9c1b112915f2d1e998a38b98f65 |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1095/biolreprod67.4.1225 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00063363_v67_n4_p1225_Ellerman |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063363_v67_n4_p1225_Ellerman |y Registro en la Biblioteca Digital |
| 961 | |a paper_00063363_v67_n4_p1225_Ellerman |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 81241 | ||