Ribosomal DNA variation in the grasshopper, Dichroplus elongatus

We report an RFLP analysis of ribosomal DNA variation in natural populations of the grasshopper, Dichroplus elongatus, previously analyzed for mitochondrial DNA variation. DNA samples were digested with five restriction enzymes, BamHI, EcoRI, HindIII, PstI, and XbaI. BamHI was the only enzyme that s...

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Autor principal: Clemente, M.
Otros Autores: Remis, M.I, Vilardi, J.C
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2002
Acceso en línea:Registro en Scopus
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024 7 |2 scopus  |a 2-s2.0-1842832983 
024 7 |2 cas  |a DNA, Ribosomal 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a GENOE 
100 1 |a Clemente, M. 
245 1 0 |a Ribosomal DNA variation in the grasshopper, Dichroplus elongatus 
260 |c 2002 
270 1 0 |m Clemente, M.; Instituto de Ingeniería Genética Y Biología Molecular (INGEBI-CONICET), Vuelta de Obligado 2490, Buenos Aires, 1428, Argentina; email: clemente@dna.uba.ar 
506 |2 openaire  |e Política editorial 
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520 3 |a We report an RFLP analysis of ribosomal DNA variation in natural populations of the grasshopper, Dichroplus elongatus, previously analyzed for mitochondrial DNA variation. DNA samples were digested with five restriction enzymes, BamHI, EcoRI, HindIII, PstI, and XbaI. BamHI was the only enzyme that showed no variation. The remaining enzymes showed fragment size variation at both intra- and interpopulation levels. Stepwise regression analysis revealed that the average number of length variants per individual is significantly associated with altitude. Moreover, the same analysis indicated that the frequency of some restriction variants exhibits a significant regression on both geographic and climatic variables. The intra- and interpopulation variability of rDNA was analysed by Lynch's and Hedrick's similarity indices using presence or absence of a fragment and band intensities, respectively. The corresponding neighbour-joining (N-J) trees based on Lynch's and Hedrick's genetic distances resulted in similar topologies. However, these trees were not in agreement with the N-J dendrogram obtained from mtDNA data previously reported by Clemente et al. (2000). The disagreement between mtDNA and rDNA phenograms along with the observed correlation between rDNA variability and geographical and climatic variables suggest some form of selection, besides genetic drift and migration, is involved in the pattern of rDNA variation. © 2002 NRC.  |l eng 
593 |a Laboratorio de Genética de Poblaciones, Facultad de Cs. Exactas Y Naturales, Ciudad Universitaria, Buenos Aires, 1428, Argentina 
593 |a Instituto de Ingeniería Genética Y Biología Molecular (INGEBI-CONICET), Vuelta de Obligado 2490, Buenos Aires, 1428, Argentina 
593 |a Consejo Nacional de Investigaciones Científicas Y Técnicas, Argentina 
690 1 0 |a DICHROPLUS ELONGATUS 
690 1 0 |a ENVIRONMENTAL VARIABLES 
690 1 0 |a RFLP 
690 1 0 |a RIBOSOMAL DNA 
690 1 0 |a DICHROPLUS ELONGATUS 
690 1 0 |a RIBOSOME DNA 
690 1 0 |a ANIMAL 
690 1 0 |a ARTICLE 
690 1 0 |a GENETICS 
690 1 0 |a GRASSHOPPER 
690 1 0 |a RESTRICTION FRAGMENT LENGTH POLYMORPHISM 
690 1 0 |a SOUTHERN BLOTTING 
690 1 0 |a ANIMALS 
690 1 0 |a BLOTTING, SOUTHERN 
690 1 0 |a DNA, RIBOSOMAL 
690 1 0 |a GRASSHOPPERS 
690 1 0 |a POLYMORPHISM, RESTRICTION FRAGMENT LENGTH 
700 1 |a Remis, M.I. 
700 1 |a Vilardi, J.C. 
773 0 |d 2002  |g v. 45  |h pp. 1125-1133  |k n. 6  |p Genome  |x 08312796  |w (AR-BaUEN)CENRE-592  |t Genome 
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