Binding of 125I-prolactin to spermatozoa from normospermic and asthenospermic men
Scatchard analysis of prolactin binding sites (PRL-BS) from ejaculated spermatozoa showed a single population of binding sites (apparent association constant: 2.51 ± 0.186 nmol/l-1) with 0.317 ± 0.0743 fmol/106 sperm binding sites. Different pools of spermatozoa were incubated with increasing concen...
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| Otros Autores: | , , , , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
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Springer International Publishing
1997
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 09941caa a22013457a 4500 | ||
|---|---|---|---|
| 001 | PAPER-20188 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518205134.0 | ||
| 008 | 190411s1997 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0031282989 | |
| 024 | 7 | |2 cas |a human growth hormone, 12629-01-5; iodine 125, 14158-31-7, 22822-81-7; prolactin, 12585-34-1, 50647-00-2, 9002-62-4; recombinant follitropin, 1219693-73-8, 146479-72-3, 1359819-75-2; tetracosactide, 16960-16-0; Adrenocorticotropic Hormone, 9002-60-2; Follicle Stimulating Hormone, 9002-68-0; Human Growth Hormone, 12629-01-5; Iodine Radioisotopes; Prolactin, 9002-62-4 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a JEIND | ||
| 100 | 1 | |a Luthy, I.A. | |
| 245 | 1 | 0 | |a Binding of 125I-prolactin to spermatozoa from normospermic and asthenospermic men |
| 260 | |b Springer International Publishing |c 1997 | ||
| 270 | 1 | 0 | |m Luthy, I.A.; Inst. Biologia Medicina Experimental, Obligado 2490, (1428) Buenos Aires, Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Shah, G.V., Desai, R.B., Sheth, A.R., Effect of prolactin on metabolism of human spermatozoa (1976) Fertil. Steril., 27, p. 1292 | ||
| 504 | |a Velázquez-Ramírez, A., Vilar-Rojas, C., Hicks, J.J., Similar effects of prolactin and dbcAMP upon human spermatozoa metabolism (1980) Int. J. Androl., 3, p. 23 | ||
| 504 | |a Sheth, A.R., Shah, G.V., Effect of prolactin on tetracycline binding to human spermatozoa (1978) Fertil. Steril., 29, p. 431 | ||
| 504 | |a Pierrepoint, C.G., John, B.M., Groom, G.V., Wilson, D.W., Gow, J.G., Prolactin and testosterone levels in the plasma of fertile and infertile men (1978) J. Endocrinol., 76, p. 171 | ||
| 504 | |a Gonzales, G.F., Garcia-Hjarles, M., Velazquez, G., Coyotupa, J., Seminal prolactin and its relationship to sperm motility in men (1989) Fertil. Steril., 51, p. 498 | ||
| 504 | |a Sheth, A.R., Mugatwala, P.P., Shah, G.V., Rao, S.S., Occurrence of prolactin in human semen (1975) Fertil. Steril., 26, p. 905 | ||
| 504 | |a Stovall, D., Shabanowitz, R., The effect of prolactin on human sperm capacitation and acrosome reaction (1991) Fertil. Steril., 56, p. 960 | ||
| 504 | |a Aiman, J., McAsey, M., Harms, L., Serum and seminal plasma prolactin concentrations in men with normozoospermia, oligospermia or azoospermia (1988) Fertil. Steril., 49, p. 133 | ||
| 504 | |a Tash, J.S., Means, A.R., Regulation of protein phosphorylation by cyclic adenosine monophosphate and calcium (1982) Biol. Reprod., 26, p. 745 | ||
| 504 | |a Costlow, M.E., McGuire, W.L., Autoradiographic localization of the binding of 125I-labelled prolactin to rat tissues in vitro (1977) J. Endocrinol., 75, p. 221 | ||
| 504 | |a Wahlström, T., Huhtaniemi, I., Hovatta, O., Seppälä, M., Localization of luteinizing hormone, follicle-stimulating hormone, prolactin, and their receptors in human and rat testis using immunohistochemistry and radioreceptor assay (1983) J. Clin. Endocrinol. Metab., 57, p. 825 | ||
| 504 | |a Charreau, E.H., Attramadal, A., Torjessen, P.A., Purvis, K., Calandra, R., Hansson, V., Prolactin binding in rat testis: Specific receptors in interstitial cells (1977) Mol. Cell. Endocrinol., 6, p. 303 | ||
| 504 | |a Ouhtit, A., Morel, G., Kelly, P.A., Visualization of gene expression of short and long forms of prolactin receptor in rat reproductive tissues (1993) Biol. Reprod., 49, p. 528 | ||
| 504 | |a Hashimoto, H., Izumi, S., Mori, C., Nakane, P., Hoshino, K., Immunohistochemical observation of prolactin receptor in rat epididymal spermatozoa by backscattered electron imaging (1988) Prolactin Gene Family and Its Receptors - Molecular Biology to Clinical Problems. Excerpta Medica International Congress Series, p. 341. , Hoshino K. (Ed.), Elsevier Science Publishers B.V. (Biomedical Division), Amsterdam | ||
| 504 | |a Hondo, E., Kurohmaru, M., Sakai, S., Ogawa, K., Hayashi, Y., Prolactin expression in rat spermatogenic cells (1995) Biol. Reprod., 52, p. 1284 | ||
| 504 | |a (1993) WHO Laboratory Manual for the Examination of Human Semen and Semen-cervical Mucus Interactions, Ed. 3, , The press syndicate of the University of Cambridge, Cambridge | ||
| 504 | |a Luthy, I.A., Calandra, R.S., Ontogeny of the sex steroid and prolactin receptors in the male rat adrenal gland (1984) Experientia, 40, p. 1002 | ||
| 504 | |a Löugren, T., Hermmilä, I., Pettersson, K., Halomen, P., Time-resolved fluorometry in immunoassays (1985) Alternative Immunoassays, p. 203. , Collins W.P., (Ed.), John Wiley & Sons, Ltd, England | ||
| 504 | |a Dowdy, S., Wearden, S., (1983) Distribution of Paired Variables. Statistics for Research, p. 201. , John Wiley & Sons, New York | ||
| 504 | |a Scherrer, B., Analyse de variance àplusieurs critères de classification (1984) Biostatistique, p. 727. , Morin G. (Ed.), Chicoutimi, Canada | ||
| 504 | |a Dowdy, S., Wearden, S., (1983) Student's t Distribution. Statistics for Research, p. 173. , John Wiley & Sons, New York | ||
| 504 | |a Scatchard, G., The attraction of proteins for small molecules and ions (1949) Ann. N.Y. Acad. Sci., 51, p. 660 | ||
| 504 | |a Calvo, J.C., Finocchiaro, L., Luthy, I., Charreau, E.H., Calandra, R.S., Engström, B., Hansson, V., Specific prolactin binding in the rat adrenal gland: Its characterization and hormonal regulation (1981) J. Endocrinol., 89, p. 317 | ||
| 504 | |a Boutin, J.M., Jolicoeur, C., Okamura, H., Gagnon, J., Edery, M., Shirota, M., Banville, Kelly, P.A., Cloning and expression of the rat prolactin receptor, a member of the growth hormone prolactin receptor gene family (1988) Cell, 53, p. 69 | ||
| 520 | 3 | |a Scatchard analysis of prolactin binding sites (PRL-BS) from ejaculated spermatozoa showed a single population of binding sites (apparent association constant: 2.51 ± 0.186 nmol/l-1) with 0.317 ± 0.0743 fmol/106 sperm binding sites. Different pools of spermatozoa were incubated with increasing concentrations of several hormones. There was a decrease in [125I]-oPRL binding with purified ovine prolactin (oPRL) and human growth hormone (hGH) which was not observed in the presence of synthetic ACTH and recombinant FSH, suggesting that binding was hormone specific. When the patient's samples were analyzed using the single point assay at saturation concentration, asthenospermic patients showed significantly higher concentration of binding sites compared to normospermic ones. Both groups of patients displayed similar PRL levels in seminal plasma measured by DELFIA. Moreover, individual values of PRL levels in seminal plasma did not correlate with PRL-BS concentrations. We thus conclude that [125I]-oPRL binding to ejaculated spermatozoa was hormone specific and with similar parameters as seen in other target tissues. PRL-BS concentration in asthenospermic patients was significantly higher than in normospermic but this was not due to different levels of PRL in seminal plasma. |l eng | |
| 593 | |a Inst. de Biol. y Med. Experimental, Hospital Carlos G. Durand, Buenos Aires, Argentina | ||
| 593 | |a Div. Endocrinología, Hospital Carlos G. Durand, Buenos Aires, Argentina | ||
| 593 | |a Cátedra de Endocrinol., Fac. Ciencias Exactas, Universidad Nacional de la Plata, La Plata, Argentina | ||
| 593 | |a Inst. de Biol. y Med. Experimental, Obligado 2490, (1428) Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a ASTHENOSPERMIC |
| 690 | 1 | 0 | |a NORMOSPERMIC |
| 690 | 1 | 0 | |a PROLACTIN RECEPTOR |
| 690 | 1 | 0 | |a REPRODUCTION |
| 690 | 1 | 0 | |a SPERMATOZOA |
| 690 | 1 | 0 | |a HUMAN GROWTH HORMONE |
| 690 | 1 | 0 | |a IODINE 125 |
| 690 | 1 | 0 | |a PROLACTIN |
| 690 | 1 | 0 | |a RECOMBINANT FOLLITROPIN |
| 690 | 1 | 0 | |a TETRACOSACTIDE |
| 690 | 1 | 0 | |a CORTICOTROPIN |
| 690 | 1 | 0 | |a DIAGNOSTIC AGENT |
| 690 | 1 | 0 | |a FOLLITROPIN |
| 690 | 1 | 0 | |a HUMAN GROWTH HORMONE |
| 690 | 1 | 0 | |a PROLACTIN |
| 690 | 1 | 0 | |a RADIOACTIVE IODINE |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a ASTHENOSPERMIA |
| 690 | 1 | 0 | |a BINDING SITE |
| 690 | 1 | 0 | |a CLINICAL ARTICLE |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a HORMONE BINDING |
| 690 | 1 | 0 | |a HUMAN |
| 690 | 1 | 0 | |a HUMAN CELL |
| 690 | 1 | 0 | |a MALE |
| 690 | 1 | 0 | |a SCATCHARD PLOT |
| 690 | 1 | 0 | |a SEMINAL PLASMA |
| 690 | 1 | 0 | |a SPERMATOZOON |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a BINDING COMPETITION |
| 690 | 1 | 0 | |a CHEMISTRY |
| 690 | 1 | 0 | |a COHORT ANALYSIS |
| 690 | 1 | 0 | |a COMPARATIVE STUDY |
| 690 | 1 | 0 | |a MALE INFERTILITY |
| 690 | 1 | 0 | |a METABOLISM |
| 690 | 1 | 0 | |a PATHOLOGY |
| 690 | 1 | 0 | |a SHEEP |
| 690 | 1 | 0 | |a SPERM |
| 690 | 1 | 0 | |a SPERMATOZOON |
| 690 | 1 | 0 | |a ADRENOCORTICOTROPIC HORMONE |
| 690 | 1 | 0 | |a ANIMALS |
| 690 | 1 | 0 | |a BINDING SITES |
| 690 | 1 | 0 | |a BINDING, COMPETITIVE |
| 690 | 1 | 0 | |a COHORT STUDIES |
| 690 | 1 | 0 | |a FOLLICLE STIMULATING HORMONE |
| 690 | 1 | 0 | |a HUMAN GROWTH HORMONE |
| 690 | 1 | 0 | |a HUMANS |
| 690 | 1 | 0 | |a INFERTILITY, MALE |
| 690 | 1 | 0 | |a IODINE RADIOISOTOPES |
| 690 | 1 | 0 | |a MALE |
| 690 | 1 | 0 | |a PROLACTIN |
| 690 | 1 | 0 | |a SHEEP |
| 690 | 1 | 0 | |a SPERMATOZOA |
| 650 | 1 | 7 | |2 spines |a SEMEN |
| 700 | 1 | |a Mormandi, E. | |
| 700 | 1 | |a Aszpis, S. | |
| 700 | 1 | |a Vázquez, S.M. | |
| 700 | 1 | |a Maccallini, G. | |
| 700 | 1 | |a Levalle, O. | |
| 700 | 1 | |a Calandra, R.S. | |
| 773 | 0 | |d Springer International Publishing, 1997 |g v. 20 |h pp. 635-639 |k n. 11 |p J. Endocrinol. Invest. |x 03914097 |t Journal of Endocrinological Investigation | |
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| 856 | 4 | 0 | |u https://doi.org/10.1007/BF03348024 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_03914097_v20_n11_p635_Luthy |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03914097_v20_n11_p635_Luthy |y Registro en la Biblioteca Digital |
| 961 | |a paper_03914097_v20_n11_p635_Luthy |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 81141 | ||