Improved bile-acid thin-layer chromatography to identify feces of neotropical carnivores

A modified thin-layer chromatography was used to identify carnivore feces based on their bile-acid patterns. Our method is safer and makes the identification of bile acids more objective. We applied the technique to feces of 2 sympatric canids in the Neotropics, the culpeo (Pseudalopex culpaeus) and...

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Autor principal: Capurro, A.F
Otros Autores: Novaro, A.J, Travaini, A., Romero, M.S
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Wiley-Blackwell 1997
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030 |a JWMAA 
100 1 |a Capurro, A.F. 
245 1 0 |a Improved bile-acid thin-layer chromatography to identify feces of neotropical carnivores 
260 |b Wiley-Blackwell  |c 1997 
270 1 0 |m Capurro, A.F.; Departamento de Investigacion, Universidad de Belgrano, Zabala 1851 Piso 12 Box 11, 1426 Buenos Aires, Argentina 
506 |2 openaire  |e Política editorial 
504 |a Aldred, D.R., (1980) Biochemical Identification of Carnivore Scats, 16p. , M.S. Thesis, Mississippi State Univ., Mississippi State, Miss 
504 |a Clinite, E.W., (1981) Biochemical Analysis of Mountain Hon and Bobcat Scats: Difference between Species and Sex, 49p. , M.S. Thesis, San Jose State Univ., San José, Calif 
504 |a Daner, D.A., Dodd, N., Comparison of coyote and grey fox scat diameters (1982) J. Wildl. Manage., 46, pp. 240-241 
504 |a Fernandez, G., Corley, J.C., Capurro, A.F., Identification of cougar and jaguar feces through bile acid thin-layer chromatography (1996) J. Wildl. Manage., 61, pp. 506-510 
504 |a Haslewood, G.A., (1967) Bile Salts, 116p. , Methuen and Co., London, U.K 
504 |a Jimenez, J.E., Yañez, J.L., Jaksic, F.M., Inability of thin-layer chromatography to distinguish feces from congeneric foxes by their bile acid contents (1996) Acta Theriol., 41, pp. 211-215 
504 |a Johnson, M.K., Aldred, D.R., Feces, bue acids and forbearers (1981) Proc. Worldwide Furbearer Conf., 2, pp. 1143-1150. , Appalachian Environ. Lab 
504 |a Clinite, E.W., Kutilek, M.J., Biochemical identification of bobcat scats (1981) Proc. Bobcat Res. Conf. Natl. Wildl. Fed. Sci. and Tech. Ser., 6, pp. 92-96 
504 |a Belden, R.C., Aldred, D.R., Differentiation of mountain lion and bobcat scats (1984) J. Wildl. Manage., 48, pp. 239-244 
504 |a Clark, T.W., Schroeder, M.H., Richardson, L., Fecal bile acids of black-footed ferrets (1986) Great Basin Nat., 8, pp. 141-144 
504 |a Kritchevsky, D., Martak, D.S., Rothblat, G.H., Detection of bile acids in thin-layer chromatography (1963) Anal. Biochem., 5, pp. 388-392 
504 |a Lin, D.S., Connor, W.E., Napton, L.K., Heizer, R.F., The steroids of 2000-year-old human coprolites (1978) J. Lipids Res., 19, pp. 215-221 
504 |a Major, M., Johnson, M.K., Davis, W.S., Kellogg, T.F., Identifying scats by recovery of bile acids (1980) J. Wildl. Manage., 44, pp. 290-293 
504 |a Medel, R., Jaksic, F.M., Ecología de los cánidos sudamericanos: Una revisión (1988) Revista Chilena de Hist. Nat., 61, pp. 67-79 
504 |a Quinn, T., Jackman, W.R., Influence of diet on detection of fecal bile acids by thin-layer chromatography (1994) J. Wildl. Manage., 58, pp. 295-299 
504 |a Taber, A.B., Novaro, A.J., Neris, N., Colman, F.H., The food habits of sympatric jaguar and puma in the Paraguayan Chaco (1997) Biotropica, 29, pp. 202-210 
504 |a Watt, M., (1987) A Scatological Analysis of Parasites and Food Habits of Jaguar (Panthera Onca) in the Cockscomb Basin of Belize, 90p. , M.S. Thesis, University of Toronto, Ont., Canada 
504 |a Weaver, J.L., Fritz, S.H., Comparison of coyote and wolf scat diameters (1979) J. Wildl. Manage., 43, pp. 786-788 
504 |a Young, C., (1978) The Effect of Cecectomy on the Fecal Steroid Excretion of the Conventional Rat, 82p. , M.S. Thesis, Mississippi State University, Mississippi State, Miss 
520 3 |a A modified thin-layer chromatography was used to identify carnivore feces based on their bile-acid patterns. Our method is safer and makes the identification of bile acids more objective. We applied the technique to feces of 2 sympatric canids in the Neotropics, the culpeo (Pseudalopex culpaeus) and South American gray fox (P. griseus), and describe bile-acid profiles of 7 other species from the region. Presence of chenodeoxycholic acid and hyodeoxycholic acids in feces of culpeo foxes allowed us to distinguish them from gray-fox feces with 85% accuracy. The method is most appropriate for carnivore species that consume small amounts of fiber and occur in dry habitats.  |l eng 
593 |a Departamento de Biología, Fac. de Ciencias Exactes y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina 
593 |a Depto.de Quimica Organica, Fac. de Ciencias Exactes y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina 
593 |a Departamento de Investigación, Universidad de Belgrano, Zabala 1851 Piso 12 Box 11, 1426 Buenos Aires, Argentina 
593 |a CONICET- Depto. Cs. Basicas, E., Universidad Nacional de Luján, CC 221, 6700 Luján, Argentina 
593 |a 201 Newins-Zeigler Hall, University of Florida, Gainesville, FL 32611, United States 
593 |a Estac. Biológica Doñana, Pabellón del Perú, Avenida María Luisa s/n, 41013 Sevilla, Spain 
690 1 0 |a CANIDS 
690 1 0 |a CULPEO 
690 1 0 |a FECAL BILE ACIDS 
690 1 0 |a FOXES 
690 1 0 |a NEOTROPICAL CARNIVORES 
690 1 0 |a PSEUDALOPEX CULPAEUS 
690 1 0 |a PSEUDALOPEX GRISEUS 
690 1 0 |a STEROID 
690 1 0 |a THIN-LAYER CHROMATOGRAPHY 
690 1 0 |a BILE ACID 
690 1 0 |a BILE ACOD 
690 1 0 |a FAECES 
690 1 0 |a IDENTIFICATION 
690 1 0 |a THIN-LAYER CHROMATOGRAPHY 
690 1 0 |a CANIDAE 
690 1 0 |a PSEUDALOPEX CULPAEUS 
690 1 0 |a PSEUDALOPEX CULPAEUS 
690 1 0 |a PSEUDALOPEX GRISEUS 
690 1 0 |a PSEUDALOPEX GRISEUS 
690 1 0 |a UROCYON CINEREOARGENTEUS 
690 1 0 |a VERTEBRATA 
651 4 |a SOUTH AMERICA 
651 4 |a SOUTH AMERICAN GRAY FOX 
700 1 |a Novaro, A.J. 
700 1 |a Travaini, A. 
700 1 |a Romero, M.S. 
773 0 |d Wiley-Blackwell, 1997  |g v. 61  |h pp. 1424-1427  |k n. 4  |p J. Wildl. Manage.  |x 0022541X  |w (AR-BaUEN)CENRE-3198  |t Journal of Wildlife Management 
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856 4 0 |u https://doi.org/10.2307/3802146  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_0022541X_v61_n4_p1424_Capurro  |y Handle 
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