A general strategy for isolation of endothelial cells from murine tissues: Characterization of two endothelial cell lines from the murine lung and subcutaneous sponge implants
A rapid, reproducible method for the isolation of murine endothelial cells (ECs) has been developed. Murine ECs were highly enriched by collagenase digestion of mechanically minced lung and subcutaneous sponge implants followed by specific selection with rat anti-mouse CD31 (ie, PECAM- 1) monoclonal...
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Lippincott Williams and Wilkins
1997
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| LEADER | 08876caa a22008057a 4500 | ||
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| 001 | PAPER-20104 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518205130.0 | ||
| 008 | 190411s1997 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-1842360394 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a ATVBF | ||
| 100 | 1 | |a Dong, Q.G. | |
| 245 | 1 | 2 | |a A general strategy for isolation of endothelial cells from murine tissues: Characterization of two endothelial cell lines from the murine lung and subcutaneous sponge implants |
| 260 | |b Lippincott Williams and Wilkins |c 1997 | ||
| 270 | 1 | 0 | |m Vecchi, A.; Ist. Ricerche Farmacol. Mario Negri, via Eritrea 62, 20157 Milano, Italy; email: vecchi@irfmn.mnegri.it |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Mantovani, A., Bussolino, F., Dejana, E., Cytokinc regulation of cndothelial cell function (1992) FASEB J, 6, pp. 2591-2599 | ||
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| 504 | |a Garlanda, C., Parravicini, C., Sironi, M., De Rossi, M., De Wainstok Calmanovici, R., Carozzi, F., Bussolino, F., Vecchi, A., Progressive growth in immunodeficient mice and host cell recruitment by mouse endothelial cells transformed by polyoma middle-sized T antigen: Implications for the pathogenesis of opportunistic vascular tumors (1994) Proc Natl Acad Sci U S A, 91, pp. 7291-7295 | ||
| 504 | |a Bocchietto, E., Guglielmetti, A., Silvagno, F., Taraboletti, G., Pescarmona, G.P., Mantovani, A., Bussolino, F., Proliferative and migratory responses of murine microvascular endothelial cells to granulocyte-colony-stimulating factor (1993) J Cell Physiol, 155, pp. 89-95 | ||
| 504 | |a Hahne, M., Jager, U., Isenmann, S., Hallmann, R., Vestweber, D., Five tumor necrosis factor-inducible cell adhesion mechanisms on the surface of mouse endothelioma cells mediate the binding of leukocytes (1993) J Cell Biol, 121, pp. 655-664 | ||
| 504 | |a O'Connell, K.A., Edidin, M., A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells (1990) J Immunol, 144, pp. 521-525 | ||
| 504 | |a Obeso, J., Weber, J., Auerbach, R., Methods in laboratory investigation: A hemangioendothelioma-derived cell line: its use as a model for study of endothelial cell biology (1990) Lab Invest, 63, pp. 259-269 | ||
| 504 | |a Sato, N., Sato, T., Takahashi, S., Kikuchi, K., Establishment of murine endothelial cell lines that develop angiosarcomas in vivo: Brief demonstration of a proposed animal model for Kaposi's sarcoma (1986) Cancer Res, 46, pp. 362-366 | ||
| 504 | |a Toda, K.-L., Tsujioka, K., Maruguchi, Y., Ishii, K., Miyachi, Y., Kuribayashi, K., Imamura, S., Establishment and characterization of a turnorisenic murine vascular endothelial cell line (F-2) (1990) Cancer Res, 50, pp. 5526-5530 | ||
| 504 | |a Dubois, N.A., Kolpack, L.C., Wang, R., Azizkhan, R.G., Bautch, V.L., Isolation and characterization of an established endothelial cell line from transgenic mouse hemangiomas (1991) Exp Cell Res, 196, pp. 302-313 | ||
| 504 | |a Auerbach, R., Lu, W.C., Pardon, E., Gumkowski, F., Kaminska, G., Kaminski, M., Specificity of adhesion between murine tumor cells and capillary endothelium: An in vitro correlate of preferential metastasis in vivo (1987) Cancer Res, 47, pp. 1492-1496 | ||
| 504 | |a Plendl, J., Hartwell, L., Auerbach, R., Organ-specific change in Dolichos biflorus lectin binding by myocardial endothelial cells during in vitro cultivation (1993) In Vitro Cell Dev Biol, 29 A, pp. 25-31 | ||
| 504 | |a Gumkowski, F., Kaminska, G., Kaminski, M., Morrissey, L.W., Auerbach, R., Heterogeneity of mouse vascular endothelium: In vitro studies of lymphatic, large blood vessel and microvascular endothelial cells (1996) Blood Vessels, 24, pp. 11-23 | ||
| 504 | |a Modzelewski, R.A., Davies, P., Watkins, S.C., Auerbach, R., Chang, M.J., Johnson, C.S., Isolation and identification of fresh tumor-derived endothelial cells from a murine RIF-1 fibrosarcoma (1994) Cancer Res, 54, pp. 336-339 | ||
| 504 | |a DeBault, L.E., Kahn, L.E., Frommes, S.P., Cancilla, P.A., Cerebral microvessels and derived cells in tissue culture: Isolation and preliminary characterization (1979) In Vitro, 15, pp. 473-487 | ||
| 504 | |a Sahdgun, G., Moore, S.A., Fabry, Z., Schelper, R.L., Hart, M.N., Purification of murine endothelial cell cultures by flow cytometry using fluorcscein-labeled Griffonia simplicifolia agglutinin (1989) Am J Pathol, 134, pp. 1227-1232 | ||
| 504 | |a Jackson, C.J., Garbett, P.K., Nissen, B., Schriebcr, L., Binding of human endothelium to Ulex europaeus I-coaled Dynabeads: Application to the isolation of microvascular endothelium (1990) J Cell Sci, 96, pp. 257-262 | ||
| 520 | 3 | |a A rapid, reproducible method for the isolation of murine endothelial cells (ECs) has been developed. Murine ECs were highly enriched by collagenase digestion of mechanically minced lung and subcutaneous sponge implants followed by specific selection with rat anti-mouse CD31 (ie, PECAM- 1) monoclonal antibody-coated magnetic beads (Dynabeads). Pure EC populations were isolated from primary cultures by a second cycle of immunomagnetic selection. The cells from the lung were then cloned by a limiting-dilution method to exclude the possibility of nonendothelial cell contamination. Of the 300 cells plated, 29 clones (≃10%) were obtained. The clones were positive for CD31 as measured by flow cytometry, and one clone from the lungs (1G11) and the cells from sponge implants (designated as SIECs) were then subjected to subsequent culture in vitro for 40 and 30 passages (up to 5 months), respectively. Characterization was performed on cells between passage 3 and 10. Both cell types formed contact-inhibited monolayers on gelatin and capillary-like 'tubes' on Matrigel. However, 1G11 cells exhibited a 'cobblestone' morphology, whereas SIECs had a fibroblast-like appearance at confluence. By flow cytometry and enzyme-linked immunosorbent assay, these cells constitutively expressed CD31. VE-cadherin (cadherin-5), CD34. [CAM-1, VCAM-1, and P-selectin. After stimulation with 30 ng/mL of tumor necrosis factor-α, the cells became positive for E-selectin (at 4 hours poststimulation) and the expression of ICAM-1, VCAM-1, and P-selectin was upregulated (after 24 hours of stimulation). The presence of VE-cadherin in 1G11 cells and SIECs was confirmed by fluorescence microscopy and Northern blot analysis. The phenotype and morphology of both cell types were stable during 5 months of culture and there was no evidence of overgrowth by contaminating cells. Taken together, the approach outlined herein may provide a general strategy for the isolation and culture of ECs from a variety of murine tissues. The general strategy outlined here is simple, effective, and flexible, allowing the inclusion of further positive or negative selection steps. |l eng | |
| 593 | |a Ist. Ric. Farmacologiche Mario Negri, Milan, Italy | ||
| 593 | |a Departamento de Quimica Biologica, Universidad de Buenos Aires, Buenos Aires, Argentina | ||
| 593 | |a Ist. Ric. Farmacologiche Mario Negri, via Eritrea 62, 20157 Milano, Italy | ||
| 690 | 1 | 0 | |a CD31 |
| 690 | 1 | 0 | |a CD34 |
| 690 | 1 | 0 | |a ENDOTHELIAL CELL LINES |
| 690 | 1 | 0 | |a MOUSE |
| 690 | 1 | 0 | |a VE-CADHERIN |
| 690 | 1 | 0 | |a ANIMAL TISSUE |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a ATHEROSCLEROSIS |
| 690 | 1 | 0 | |a CELL ADHESION |
| 690 | 1 | 0 | |a CELL CULTURE |
| 690 | 1 | 0 | |a CELL ISOLATION |
| 690 | 1 | 0 | |a MOUSE |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a VASCULAR ENDOTHELIUM |
| 700 | 1 | |a Bernasconi, S. | |
| 700 | 1 | |a Lostaglio, S. | |
| 700 | 1 | |a Wainstok De Calmanovici, R. | |
| 700 | 1 | |a Martin-Padura, I. | |
| 700 | 1 | |a Breviario, F. | |
| 700 | 1 | |a Garlanda, C. | |
| 700 | 1 | |a Ramponi, S. | |
| 700 | 1 | |a Mantovani, A. | |
| 700 | 1 | |a Vecchi, A. | |
| 773 | 0 | |d Lippincott Williams and Wilkins, 1997 |g v. 17 |h pp. 1599-1604 |k n. 8 |p ARTERIOSCLER. THROMB. VASC. BIOL. |x 10795642 |t Arteriosclerosis, Thrombosis, and Vascular Biology | |
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| 856 | 4 | 0 | |u https://doi.org/10.1161/01.ATV.17.8.1599 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_10795642_v17_n8_p1599_Dong |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10795642_v17_n8_p1599_Dong |y Registro en la Biblioteca Digital |
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| 999 | |c 81057 | ||