Immunization with recombinant Trypanosoma cruzi ribosomal P2β protein induces changes in the electrocardiogram of immunized mice
Molecular expression cloning techniques revealed that patients with severe chronic Chagas heart disease showed a strong humoral response against the cloned C-terminal portion of the Trypanosoma cruzi ribosomal P2β protein, previously named JL5. The main linear epitope of this polypeptide was mapped...
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1997
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| LEADER | 13305caa a22013097a 4500 | ||
|---|---|---|---|
| 001 | PAPER-20073 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518205128.0 | ||
| 008 | 190411s1997 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0343924502 | |
| 024 | 7 | |2 cas |a Antibodies, Protozoan; Autoantibodies; Carrier Proteins; L12E protein, Trypanosoma cruzi; maltose-binding protein; Protozoan Proteins; Recombinant Fusion Proteins; Ribosomal Proteins | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a FIMIE | ||
| 100 | 1 | |a Lopez Bergami, P. | |
| 245 | 1 | 0 | |a Immunization with recombinant Trypanosoma cruzi ribosomal P2β protein induces changes in the electrocardiogram of immunized mice |
| 260 | |c 1997 | ||
| 270 | 1 | 0 | |m Levin, M.J.; IIIGBM, INGEBI, Obligado 2490, 1428 Buenos Aires, Argentina |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Cabeza Meckert, P.M., Chambó, J., Laguens, R., Presence of cells producing antiheart autoantibodies in the inflammatory infiltrate of chronic chagasic myocarditis (1991) Clin. Immunol. Immunopathol., 60, pp. 137-144 | ||
| 504 | |a Rassi, A., Luquetti, A., Rassi, A.J., Rassi, S., Rassi, A.G., Chagas disease, clinical features (1992) Chagas Disease: Its Impact on Transfusion and Clinical Medicine, pp. 81-101. , Wendel, S., Brener, Z., Camargo, M.E. and Rassi, A., Eds. ISBT Brasil '92-SBHH (Sociedade Brazileira de Hematologia e Hemoterapia), Sao Paulo, Brazil | ||
| 504 | |a Laguens, R.P., Cabeza Meckert, P., Basombrío, M., Chambo, G., Cossio, P., Arana, R., Gelpi, R., Chronic infection of the mouse with Trypanosoma cruzi: An experimental model for Chagas disease (1980) Medicina, 40 (1 SUPPL.), pp. 33-39. , Buenos Aires | ||
| 504 | |a Andrade, S.G., Sadigursky, M., The conduction system of the heart in mice chronically infected with Trypanosoma cruzi: Histopathological lesions and electrocardiographic correlations (1987) Mem. Inst. Osw. Cruz., 82 (1), pp. 59-66 | ||
| 504 | |a Postam, M., Bailey, J., Dvorak, J., McDaniel, J., Pottala, E., Studies of Trypanosoma cruzi clones in inbred mice. III. Histopathological and electrocardiographical responses to chronic infection (1987) Am. J. Trop. Med. Hyg., 37 (3), pp. 541-549 | ||
| 504 | |a Levin, M.J., Mesri, E., Benarous, R., Levitus, G., Schijman, A., Levy-Yeyati, P., Chiale, P., Segura, E.L., Identification of major Trypanosoma cruzi antigenic determinants in chronic Chagas' heart disease (1989) Am. J. Trop. Med. Hyg., 41, pp. 530-539 | ||
| 504 | |a Levin, M.J., Levitus, G., Kerner, N., Lafón, S., Schijman, A., Levy-Yeyati, P., Finkielstein, C., Hontebeyrie-Joskowicz, M., Autoantibodies in Chagas heart disease: Possible markers of severe heart complaint (1990) Mem. Inst. Oswaldo Cruz, 85, pp. 539-543 | ||
| 504 | |a Levin, M.J., Molecular mimicry and Chagas heart disease: High anti-R13 autoantibody levels are markers of severe heart complaint (1991) Res. Immunol., 142, pp. 157-159 | ||
| 504 | |a Schijman, A.G., Dusetti, N.J., Vazquez, M.P., Lafon, S., Levy-Yeyati, P., Levin, M.J., Nucleotide cDNA and complete deduced amino acid sequence of a Trypanosoma cruzi P ribosomal protein (P-JL5) (1990) Nucleic Acids Res., 18, p. 3399 | ||
| 504 | |a Schijman, A., Vásquez, M., Ben Dov, C., Ghío, S., Lorenzi, H., Levin, M.J., Cloning and sequence analysis of the TcP2β cDNA variants of Trypanosoma cruzi (1995) Biochim. Biophys. Acta, 1264, pp. 15-18 | ||
| 504 | |a Mesri, E.A., Levitus, G., Hontebeyrie-Joskowicz, M., Van Regenmortel, M.H.V., Levin, M.J., A major Trypanosoma cruzi antigenic determinant in Chagas heart disease shares homology with the systemic lupus erythematosus ribosomal P protein epitope (1990) J. Clin. Microbiol., 28, pp. 1219-1224 | ||
| 504 | |a Levitus, G., Hontebeyrie-Joskowicz, M., Van Regenmortel, M.H.V., Levin, M.J., Humoral autoimmune response to ribosomal P proteins in chronic Chagas heart disease (1991) Clin. Exp. Immunol., 85, pp. 413-417 | ||
| 504 | |a Kaplan, D., (1995) Antígenos, Epítopes y Autoepítopes en la Infección por Trypanosoma Cruzi: Autoinmunidad en la Enfermedad de Chagas vs. Autoinmunidad Sistémica, , PhD Thesis, University of Buenos Aires, Buenos Aires | ||
| 504 | |a Levin, M.J., Kaplan, D., Ferrari, I., Arteman, P., Vazquez, M., Panebra, A., Humoral autoimmune response in Chagas disease: Trypanosoma cruzi ribosomal antigens (1993) FEMS Immunol. Med. Microbiol., 7, pp. 205-211 | ||
| 504 | |a Aznar, C., Lopez Bergami, P., Brandariz, S., Mariette, C., Liegeard, P., De Deus Alves, M.C., Luna Barreiro, E., Hontebeyrie-Joskowicz, M., Prevalence of anti-R13 antibodies in human Trypanosoma cruzi infection (1995) FEMS Immunol. Med. Microbiol., 12, pp. 231-238 | ||
| 504 | |a Ruiz, A.M., Esteva, M., Cabeza Meckert, P., Laguens, R.P., Segura, E.L., Protective immunity and pathology induced by inoculation of mice with different subcellular fractions of Trypanosoma cruzi (1985) Acta Trop., 42, pp. 299-309 | ||
| 504 | |a Levy Yeyati, P., (1992) Antígenos del Estadío Trypomastigote Sanguíneo de la Cepa RA1 de Trypanosoma Cruzi y de la Cepa Boitron de Leishmania Braziliensis Braziliensis: Respuesta Humoral, Anti-HSP70 en la Enfermedad de Chagas y Leishmaniasis, , PhD Thesis, University of Buenos Aires, Buenos Aires | ||
| 504 | |a Levy Yeyati, P., Bonnefoy, S., Mirkin, G., Debrabant, A., Lafon, S., Panebra, A., Gonzalez-Cappa, E., Levin, M.J., The 70-kDa heat-shock protein is a major antigenic determinant in human Trypanosoma cruzi/Leishmania braziliensis braziliensis mixed infection (1991) Immunol. Lett., 31, pp. 27-34 | ||
| 504 | |a Levin, M.J., Rossi, R., Levitus, G., Mesri, E., Bonnefoy, S., Kerner, N., Hontebeyrie-Joskowicz, M., The cloned C-terminal region of a Trypanosoma cruzi P ribosomal P protein harbors two antigenic determinants (1990) Immunol. Lett., 24, pp. 69-74 | ||
| 504 | |a Celentano, A., Gonzalez Cappa, E., In vivo macrophage function in experimental infection with Trypanosoma cruzi subpopulations (1993) Acta Trop., 55, pp. 171-180 | ||
| 504 | |a Teixeira, A.R.L., Santos-Buch, C.A., The immunology of experimental Chagas disease. I. Preparation of Trypanosoma cruzi antigens and humoral antibody response to these antigens (1974) J. Immunol., 113, pp. 859-869 | ||
| 504 | |a Teixeira, A.R.L., Teixeira, M.L., Santos-Buch, C.A., The immunology of experimental Chagas disease. IV. Production of lesions in rabbits similar to those of chronic Chagas disease in man (1975) Am. J. Pathol., 80, pp. 163-179 | ||
| 504 | |a Lopetegui, R., Sosa Miatello, C., La Via, M.I., Antígenos ribosomales de Trypanosoma cruzi. Efecto de ribonucleasa sobre su reactividad antigenica (1980) Medicina, 40, pp. 91-96. , Buenos Aires | ||
| 504 | |a Lopetegui, R., Sosa Miatello, C., Fiorotto, E.R., Trypanosoma cruzi and mouse myocardium ribosomes. Antibody detection in experimental Chagas infection (1983) Comm. Biol., 2, pp. 79-85 | ||
| 504 | |a Lopetegui, R., Sosa Miatello, C., Fiorotto, E.R., Anti-ribosome humoral antibodies elicited in Trypanosoma cruzi infected mice (1983) Comm. Biol., 2, pp. 195-202 | ||
| 504 | |a Sosa Miatello, C., Fiorotto, E.R., Ribosomal antibody response in rabbits and mice infected with Trypanosoma cruzi (1989) Rev. Argent. Microbiol., 32, pp. 141-145 | ||
| 504 | |a Hines, J.J., Weissbach, H., Brot, N., Elkon, K., Anti-P autoantibody production requires P1/P2 as immunogens but is not driven by exogenous self-antigen in MRL mice (1991) J. Immunol., 146, pp. 3386-3395 | ||
| 504 | |a Bestetti, R.B., Role of parasites in the pathogenesis of Chagas' cardiomyopathy (1996) Lancet, 347, pp. 913-914 | ||
| 504 | |a Campos De Carvalho, A.C., Levin, M.J., Farias De Oliveira, S., Nascimento, J.H.M., Pedrosa, R.C., Lopez Bergami, P., Ferrari, I., Masuda, M.O., Possible involvement of anti-ribosomal Tc P protein response in the cardiac conduction disturbances of chronic Chagas disease (1995) Mem. Inst. Osw. Cruz., 90 (1 SUPPL.), p. 151 | ||
| 520 | 3 | |a Molecular expression cloning techniques revealed that patients with severe chronic Chagas heart disease showed a strong humoral response against the cloned C-terminal portion of the Trypanosoma cruzi ribosomal P2β protein, previously named JL5. The main linear epitope of this polypeptide was mapped to the 13 C-terminal amino acid sequence EEEDDDMGFGLFD (named R13), which is almost identical to the mammalian ribosomal P consensus sequence EESDDDMGFGLFD (named H13). Enzyme-linked immunosorbent assay measurements demonstrated that sera from patients with chronic Chagas heart disease presented a very specific anti-P humoral response with high anti- R13, but low H13 antibody levels. We attempted to develop an animal model that would reproduce, at least partially, two features of the human infection: (1) the serological pattern of the anti-P response, and (2) specific cardiac symptoms. To this effect, mice were immunized with T. cruzi P2β recombinant protein. Immunization reproduced the typical anti-P antibody profile defined for chronic infections, but did not induce cardiac inflammatory lesions. However, it altered significantly the electrocardiograms of immunized mice. It is suggested that this assay represents a functional test for assessing the biological activity of antibodies against T. cruzi ribosomal P protein on cardiac muscle. |l eng | |
| 536 | |a Detalles de la financiación: 936018 AR;ARG/B7-3011/94/28A | ||
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires, Ex 283 | ||
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires | ||
| 536 | |a Detalles de la financiación: Ministère des Affaires Etrangères | ||
| 536 | |a Detalles de la financiación: This work was supported by the French-Argentinean Cooperation Program (CNRS-CONICET, Ambassade de France en Argentine), Ministère des Affaires Etrangeres, Paris, France), Fundación Antorchas, Universidad de Buenos Aires Ex 283, a grant of the Comisión de Investigaciones Cientı́ficas de la Provincia de Buenos Aires, Argentina and European Commission contract No. 936018 AR;ARG/B7-3011/94/28A. | ||
| 593 | |a Inst. Invest. Ing. Genet. y Biol. M., Obligado 2490, 1428 Buenos Aires, Argentina | ||
| 593 | |a Inst. Cardiol. y Cirugia Cardiovasc., Fundación Favaloro, Belgrano 1746, 1093 Buenos Aires, Argentina | ||
| 593 | |a Comn. Invest. Cie. Provincia B., Buenos Aires, Argentina | ||
| 593 | |a Deutsches RheumaForschungsZentrum, Monbijoustraße 2A, D-10117 Berlin, Germany | ||
| 593 | |a Inst. de Biologie Moléculaire, Cellulaire UPR 9021 CNRS, 67084 Strasbourg Cedex, France | ||
| 690 | 1 | 0 | |a CHAGAS DISEASE |
| 690 | 1 | 0 | |a ELECTROCARDIOGRAM |
| 690 | 1 | 0 | |a FUNCTIONAL ASSAY |
| 690 | 1 | 0 | |a HEART |
| 690 | 1 | 0 | |a IMMUNIZATION |
| 690 | 1 | 0 | |a RIBOSOMAL P PROTEIN |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a ANIMAL MODEL |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CHAGAS DISEASE |
| 690 | 1 | 0 | |a ELECTROCARDIOGRAM |
| 690 | 1 | 0 | |a ENZYME LINKED IMMUNOSORBENT ASSAY |
| 690 | 1 | 0 | |a HUMAN |
| 690 | 1 | 0 | |a HUMORAL IMMUNITY |
| 690 | 1 | 0 | |a IMMUNIZATION |
| 690 | 1 | 0 | |a MOUSE |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a ANIMALS |
| 690 | 1 | 0 | |a ANTIBODIES, PROTOZOAN |
| 690 | 1 | 0 | |a AUTOANTIBODIES |
| 690 | 1 | 0 | |a CARRIER PROTEINS |
| 690 | 1 | 0 | |a ELECTROCARDIOGRAPHY |
| 690 | 1 | 0 | |a HEART |
| 690 | 1 | 0 | |a HUMANS |
| 690 | 1 | 0 | |a IMMUNIZATION |
| 690 | 1 | 0 | |a MICE |
| 690 | 1 | 0 | |a MICE, INBRED BALB C |
| 690 | 1 | 0 | |a MYOCARDIUM |
| 690 | 1 | 0 | |a PROTOZOAN PROTEINS |
| 690 | 1 | 0 | |a RECOMBINANT FUSION PROTEINS |
| 690 | 1 | 0 | |a RIBOSOMAL PROTEINS |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 700 | 1 | |a Cabeza Meckert, P. | |
| 700 | 1 | |a Kaplan, D. | |
| 700 | 1 | |a Levitus, G. | |
| 700 | 1 | |a Elias, F. | |
| 700 | 1 | |a Quintana, F. | |
| 700 | 1 | |a Van Regenmortel, M. | |
| 700 | 1 | |a Laguens, R. | |
| 700 | 1 | |a Levin, M.J. | |
| 773 | 0 | |d 1997 |g v. 18 |h pp. 75-85 |k n. 1 |p FEMS IMMUNOL. MED. MICROBIOL. |x 09288244 |w (AR-BaUEN)CENRE-4726 |t FEMS Immunology and Medical Microbiology | |
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| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_09288244_v18_n1_p75_LopezBergami |y Handle |
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