Rat harderian gland porphobilinogen deaminase: Characterization studies and regulatory action of protoporphyrin IX
Properties of purified porphobilinogen deaminase (PBG-D; EC 4.3.1.8) from rat harderian gland are here presented. The enzyme behaves as a monomer of M(r) 38 ± 2 kDa and is optimally active at pH 8.0-8.2. Its activation energy, determined by an Arrhenius plot, is 76.1 kJ/mol. Initial velocity studies...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
Academic Press Inc.
1997
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 09030caa a22011777a 4500 | ||
|---|---|---|---|
| 001 | PAPER-20022 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518205124.0 | ||
| 008 | 190411s1997 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0031282308 | |
| 024 | 7 | |2 cas |a 5,5' dithiobis(2 nitrobenzoic acid), 69-78-3; cysteine, 4371-52-2, 52-89-1, 52-90-4; n ethylmaleimide, 128-53-0; porphobilinogen deaminase, 9036-47-9, 9074-91-3; protoporphyrin, 553-12-8; uroporphyrin, 18273-06-8, 26316-36-9, 553-18-4, 607-14-7 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a ABBIA | ||
| 100 | 1 | |a Cardalda, C.A. | |
| 245 | 1 | 0 | |a Rat harderian gland porphobilinogen deaminase: Characterization studies and regulatory action of protoporphyrin IX |
| 260 | |b Academic Press Inc. |c 1997 | ||
| 270 | 1 | 0 | |m Batlle, A.Viamonte 1881, 10 Piso, 1656 Buenos Aires, Argentina; email: cipyp@alad.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Kennedy, G.Y., (1970) Comp. Biochem. Physiol., 36, pp. 21-36 | ||
| 504 | |a Sakai, T., (1981) Arch. Histol. Jpn., 44, pp. 299-333 | ||
| 504 | |a Payne, A.P., (1994) J. Anat., 185, pp. 1-49 | ||
| 504 | |a Spike, R.C., Johnston, H.S., McGadey, J., Moore, M.R., Thompson, G.G., Payne, A.P., (1986) J. Anat., 145, pp. 67-77 | ||
| 504 | |a Tomio, J.M., Grinstein, M., (1968) Eur. J. Biochem., 6, pp. 80-83 | ||
| 504 | |a Carriere, R., (1985) Anat. Rec., 213, pp. 496-504 | ||
| 504 | |a Spike, R.C., Payne, A.P., Thompson, G.G., Moore, M.R., (1990) Biochim. Biophys. Acta, 1034, pp. 1-3 | ||
| 504 | |a Webb, S.M., Hoffman, R.A., Puig-Domingo, M.L., Reiter, R.J., (1992) Harderian Glands: Porphyrin Metabolism, Behavioral and Endocrine Effects, , Berlin/New York: Springer Verlag | ||
| 504 | |a Moore, M.R., Thompson, G.G., Payne, A.P., McGadey, J., (1980) Int. J. Biochem., 12, pp. 501-504 | ||
| 504 | |a Lin, W.L., Nadakavukaren, M.J., (1982) Comp. Biochem. Physiol., 71, pp. 539-540 | ||
| 504 | |a Elder, G.H., (1976) Essays Med. Biochem., 2, pp. 75-114 | ||
| 504 | |a Jordan, P.M., Warren, M.J., (1987) FEBS Lett., 225, pp. 87-92 | ||
| 504 | |a Hart, G.J., Miller, A.D., Leeper, F.G., Battersby, A.R., (1987) J. Chem. Soc. Chem. Commun., pp. 1762-1765 | ||
| 504 | |a Miller, A.D., Hart, G.J., Packman, L.C., Battersby, A.R., (1988) Biochem. J., 254, pp. 915-918 | ||
| 504 | |a Gellerfors, P.L., Saltzgaber-Muller, J., Douglas, M.G., (1986) Biochem. J., 240, pp. 673-677 | ||
| 504 | |a Thomas, S.D., Jordan, P.M., (1986) Nucleic Acids Res., 14, pp. 6215-6226 | ||
| 504 | |a Alefounder, P.A., Abell, C., Battersby, A.R., (1988) Nucleic Acids Res., 16, p. 9871 | ||
| 504 | |a Raich, N., Romeo, P.H., Dubart, A., Beaupain, D., Cohen-Sola, I.M., Goossens, M., (1986) Nucleic Acids Res., 15, pp. 5955-5968 | ||
| 504 | |a Beaumont, C., Porcher, C., Picat, C., Nordmann, Y., Grandchamp, B., (1989) J. Biol. Chem., 264, pp. 14829-14834 | ||
| 504 | |a Sharif, A.L., Smith, A.G., Abell, C., (1989) Eur. J. Biochem., 184, pp. 353-359 | ||
| 504 | |a Witty, M., Wallace-Cook, A., Albrecht, H., Spano, A., Michel, H., Shabanowitz, J., Hunt, D., Smith, A.G., (1993) Plant Physiol., 103, pp. 139-147 | ||
| 504 | |a Lim, S.H., Witty, M., Wallace-Cook, A.D., Ilag, L.Y., Smith, A.G., (1994) Plant Mol. Biol., 26, pp. 863-872 | ||
| 504 | |a Sancovich, H.A., Ferramola, A.M., Batlle, A.M., Del, C., Grinstein, M., (1970) Methods in Enzymology, pp. 220-222. , H. Tabor, & C.W. Tabor. New York/London: Academic Press | ||
| 504 | |a Rimington, C., (1960) Biochem. J., 75, pp. 620-623 | ||
| 504 | |a Seubert, S., Seubert, A., (1989) Merck Spectr., 2, pp. 8-9 | ||
| 504 | |a Lim, C.K., Rideout, J.M., Wright, D.J., (1983) Biochem. J., 211, pp. 435-438 | ||
| 504 | |a Bradford, M.M., (1976) Anal. Biochem., 72, pp. 248-254 | ||
| 504 | |a Williams, D.C., Morgan, C.S., McDonald, E., Battersby, A.R., (1981) Biochem. J., 193, pp. 301-310 | ||
| 504 | |a Shoi, Y., Nagamine, M., Kuraki, M., Sasa, T., (1980) Biochim. Biophys. Acta, 616, pp. 300-309 | ||
| 504 | |a Spano, A.J., Timko, M.P., (1991) Biochim. Biophys. Acta, 1076, pp. 29-36 | ||
| 504 | |a Jones, R.M., Jordan, P.M., (1994) Biochem. J., 299, pp. 895-902 | ||
| 504 | |a Juknat, A.A., Doernemann, D., Senger, H., (1994) Planta, 193, pp. 123-130 | ||
| 504 | |a Anderson, P.M., Desnick, R.J., (1980) J. Biol. Chem., 255, pp. 1993-1999 | ||
| 504 | |a Williams, D.C., (1984) Biochem. J., 217, pp. 675-683 | ||
| 504 | |a Battersby, A.R., Fookes, C.J.R., Hart, G., Matcham, G.W.J., Pandey, P.S., (1983) J. Chem. Soc. Perkin Trans. I, pp. 3041-3047 | ||
| 504 | |a Elliot, K.R., Tipton, K.F., (1974) Biochem. J., 141, pp. 789-805 | ||
| 504 | |a Cleland, W.W., (1963) Biochim. Biophys. Acta, 67, pp. 104-172 | ||
| 504 | |a Battersby, A.R., Fookes, C.J.R., Matcham, G.W.J., McDonald, E., Hollenstein, R., (1983) J. Chem. Soc. Perkin Trans. I, pp. 3031-3040 | ||
| 504 | |a Tipton, K.F., Dixon, H.B.F., (1979) Methods Enzymol., 63, pp. 183-234 | ||
| 504 | |a Hart, G.J., Miller, A.D., Battersby, A.R., (1988) Biochem. J., 252, pp. 909-912 | ||
| 504 | |a Jordan, P.M., Warren, M.J., Williams, H.J., Stolowich, N.J., Roessner, C.A., Grant, S.K., Scott, A.I., (1988) FEBS Lett., 235, pp. 189-193 | ||
| 504 | |a Lander, M., Pitt, A.R., Alefounder, P.R., Bardy, D., Abell, C., Battersby, A., (1991) Biochem. J., 275, pp. 447-452 | ||
| 504 | |a Jordan, P.M., Woodcock, S.C., (1991) Biochem. J., 280, pp. 445-449 | ||
| 504 | |a Meissner, P., Adams, P., Kirsch, R., (1993) J. Clin. Invest., 91, pp. 1436-1444 | ||
| 520 | 3 | |a Properties of purified porphobilinogen deaminase (PBG-D; EC 4.3.1.8) from rat harderian gland are here presented. The enzyme behaves as a monomer of M(r) 38 ± 2 kDa and is optimally active at pH 8.0-8.2. Its activation energy, determined by an Arrhenius plot, is 76.1 kJ/mol. Initial velocity studies showed a linear progress curve for uroporphyringen I formation and a hyperbolic dependence of the initial rate on substrate concentration, indicating the existence of a sequential displacement mechanism. Apparent kinetic constants, K(m) and V(m), calculated at 37°C and pH 8.0 were 1.1 μM and 170 pmol/min mg, respectively. The pH dependence of the apparent kinetic parameters revealed the ionization of residues with pK(A)/(ES) and pK(B)/(ES) of 7.4 ± 0.1 and 8.6 ± 0.1, respectively, and a pK(E) value of 8.0 ± 0.1. Incubation of PBG-D with 5.0 mM N-ethylmaleimide and 5.0 mM 5,5'- dithiobis(2-nitrobenzoic acid) at pH 8.0 led to inhibitions of 70 and 50%, respectively. The effect of pH, as well as the effect of thiol reagents, on enzyme activity strongly suggests the involvement of cysteine residue(s) in the mechanism of uroporphyrinogen I biosynthesis, in both the catalytic reaction and the substrate binding. Rat harderian gland PBG-D activity decreased with increasing concentrations of protoporphyrin IX, reaching a 40% inhibition at the in vivo concentration of the porphyrin and 7 μM PBG. Even at saturating concentrations of substrate, inhibition by protoporphyrin was not completely reversed. So, accumulated porphyrin may act as an regulator of PBG-D activity in rat harderian gland. |l eng | |
| 593 | |a Ctro. de Invest. Sobre Porfirinas Y., Departamento de Quimica Biologica, Univ. de Buenos Aires Cd. Univ., 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a CYSTEINE RESIDUE(S) |
| 690 | 1 | 0 | |a PORPHOBILINOGEN DEAMINASE |
| 690 | 1 | 0 | |a PORPHOBILINOGEN DEAMINASE REGULATION |
| 690 | 1 | 0 | |a PROTOPORPHYRIN IX |
| 690 | 1 | 0 | |a RAT HARDERIAN GLAND |
| 690 | 1 | 0 | |a 5,5' DITHIOBIS(2 NITROBENZOIC ACID) |
| 690 | 1 | 0 | |a CYSTEINE |
| 690 | 1 | 0 | |a N ETHYLMALEIMIDE |
| 690 | 1 | 0 | |a PORPHOBILINOGEN DEAMINASE |
| 690 | 1 | 0 | |a PROTOPORPHYRIN |
| 690 | 1 | 0 | |a UROPORPHYRIN |
| 690 | 1 | 0 | |a ANIMAL TISSUE |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CATALYSIS |
| 690 | 1 | 0 | |a ENZYME ACTIVITY |
| 690 | 1 | 0 | |a ENZYME ANALYSIS |
| 690 | 1 | 0 | |a ENZYME KINETICS |
| 690 | 1 | 0 | |a ENZYME PURIFICATION |
| 690 | 1 | 0 | |a ENZYME SUBSTRATE |
| 690 | 1 | 0 | |a HARDER GLAND |
| 690 | 1 | 0 | |a MALE |
| 690 | 1 | 0 | |a MOLECULAR WEIGHT |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a RAT |
| 700 | 1 | |a Juknat, A.A. | |
| 700 | 1 | |a Princ, F.G. | |
| 700 | 1 | |a Batlle, A. | |
| 773 | 0 | |d Academic Press Inc., 1997 |g v. 347 |h pp. 69-77 |k n. 1 |p ARCH. BIOCHEM. BIOPHYS. |x 00039861 |w (AR-BaUEN)CENRE-1377 |t Archives of Biochemistry and Biophysics | |
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| 856 | 4 | 0 | |u https://doi.org/10.1006/abbi.1997.0310 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00039861_v347_n1_p69_Cardalda |y Handle |
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