Resistance Against Pepper Severe Mosaic Potyvirus in Transgenic Tobacco Plants

The coat protein gene of the pepper severe mosaic poty-virus was introduced into tobacco plants. Several transgenic lines were assayed for virus resistance under greenhouse conditions. Line 232, showing higher levels of resistance than other transgenic plants, was studied in further detail. Upon cha...

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Autor principal: Rabinowicz, P.D
Otros Autores: Bravo-Almonacid, F.F, Lampasona, S., Rodríguez, F., Gracia, O., Mentaberry, A.N
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Blackwell Verlag GmbH Berlin 1998
Acceso en línea:Registro en Scopus
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030 |a JPHYE 
100 1 |a Rabinowicz, P.D. 
245 1 0 |a Resistance Against Pepper Severe Mosaic Potyvirus in Transgenic Tobacco Plants 
260 |b Blackwell Verlag GmbH Berlin  |c 1998 
270 1 0 |m Mentaberry, A.N.; Inst. Invest. Ing. Genet. Biol. M., Vuelta de Obligado 2490, 1428, Buenos Aires, Argentina 
506 |2 openaire  |e Política editorial 
504 |a Audy, P., Palukaitis, P., Slack, S., Zaitlin, M., Replicase mediated resistance to potato virus Y in transgenic plants (1994) Mol. Plant Microbe Interact., 7, pp. 15-22 
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504 |a Beachy, R., Loesch-Fries, S., Turner, N., Coat protein-mediated resistance against virus infection (1990) Ann. Rev. Phytopathol., 28, pp. 451-474 
504 |a Clark, M.F., Adams, A.N., Characteristics of the microplate method of enzyme-linked immunoabsorbent assay for the detection of plant viruses (1977) J. Gen. Virol., 34, pp. 475-483 
504 |a Dellaporta, S.L., Wood, J., Hicks, J.B., A plant DNA minipreparation: Version II (1983) Plant Mol Biol. Rep, 1, pp. 19-21 
504 |a Díaz, I., Moreno, R., Power, J.B., Plant regeneration from protoplasts of Capsicum annuum (1988) Plant Cell Rep., 7, pp. 210-212 
504 |a Dougherty, G., Lindbo, J.A., Smith, H.A., Parks, T.D., Swaney, S., Proebsting, W.M., RNA-mediated virus resistance in transgenic plants: Exploitation of a cellular pathway possibly involved in RNA degradation (1994) Mol. Plant Microbe Interact., 7, pp. 544-552 
504 |a Dumas De Vaulx, P., Chambonnet, R.D., Sibi, M., (1982) Variability in Plants Regenerated from Tissue Culture, p. 92. , Earle, E. D. and Y. Demarly (eds), Praeger Publishers, New York 
504 |a Feinberg, A.P., Vogelstein, B., A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity (1983) Anal Biochem., 132, pp. 6-13 
504 |a Feldman, J.M., Gracia, O., Pepper Severe Mosaic Virus: A new potyvirus from pepper in Argentina (1977) Phytopathol. Z., 89, pp. 146-160 
504 |a Fitchen, J.H., Beachy, R.N., Genetically engineered protection against viruses in transgenic plants (1993) Ann. Rev. Microbiol., 47, pp. 739-763 
504 |a Horsh, R., Fry, J., Hoffmann, N., Eicholtz, D., Rogers, S., Fraley, R., A simple and general method for transferring genes into plants (1985) Science, 227, pp. 1229-1231 
504 |a Jefferson, R.A., Kavanagh, T.A., Bevan, M.W., GUS fusions: Beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants (1987) EMBO J., 6, pp. 3901-3907 
504 |a Koncz, C., Schell, J., The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimeric genes carried by a novel type of Agrobacterium binary vector (1986) Mol. Gen. Genet., 204, pp. 383-393 
504 |a Lindbo, A., Dougherty, W.G., Untranslatable transcripts of the tobacco etch virus coat protein gene sequence can interfere with tobacco etch virus replication in transgenic plants and protoplasts (1992) Virology, 189, pp. 725-733 
504 |a Lindbo, J., Silva-Rosales, L., Dougherty, W., Pathogen derived resistance to potyviruses: Working but why? (1993) Semin. Virol., 4, pp. 369-379 
504 |a Liu, W., Parrot, W.A., Hildebrand, D.F., Collins, G.B., Williams, E.G., Agrobacterium induced gall formation in bell pepper (Capsicum annuum L.) and formation of shoot like structures expressing introduced genes (1990) Plant Cell Rep., 9, pp. 360-364 
504 |a Murashige, T., Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue culture (1962) Physiol. Plant., 15, pp. 473-497 
504 |a Ochoa-Alejo, N., Ireta-Moreno, L., Cultivar differences in shoot-forming capacity of hypocotyl tissues of chilli pepper (Capsicum annuum L.) cultured in vitro (1990) Sci. Hort., 42, pp. 21-28 
504 |a Rabinowicz, P.D., Bravo-Almonacid, F., Mentaberry, A.N., Nucleotide cDNA sequence coding for the PeSMV coat protein and the 3 region (1993) Plant Physiol., 103, p. 1023 
504 |a Riechmann, J., Lain, S., Garcia, J., Highlights and prospects of potyvirus molecular biology (1992) J. Gen. Virol., 73, pp. 1-16 
504 |a Robaglia, C., Durand-Tardiff, M., Tronchet, M., Boudazin, G., Astier-Manifacier, S., Casse-Delbart, F., Nucleotide sequence of potato virus Y (N strain) genomic RNA (1989) J. Gen. Virol., 70, pp. 935-947 
504 |a Sambrook, J., Fritsch, T.E., Maniatis, T., (1989) Molecular Cloning. A Laboratory Manual, , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY 
504 |a Smith, H., Swaney, S., Parks, D., Wernsman, E., Dougherty, W., Transgenic plants virus resistant mediated by untranslatable sense RNA: Expression, regulation, and fate of nonessential RNAs (1994) Plant Cell, 6, pp. 1441-1453 
504 |a Vardi, E., Sela, I., Edelbaum, O., Livneh, O., Kuznetsova, L., Stram, Y., Plants transformed with a cistron of potato virus Y protease (NIa) are resistant to virus-infection (1993) Proc. Natl. Acad. Sci. USA, 90, pp. 7513-7517 
520 3 |a The coat protein gene of the pepper severe mosaic poty-virus was introduced into tobacco plants. Several transgenic lines were assayed for virus resistance under greenhouse conditions. Line 232, showing higher levels of resistance than other transgenic plants, was studied in further detail. Upon challenge with PepSMV, R 2 descendants from this line were monitored for virus accumulation for a period of 45 days. Virus titre was lower in plants of the line 232 than in control plants throughout the experiment. At 14 days post-inoculation the transgenic plant line reached the maximum virus accumulation measured by ELISA. Those plants also showed a 1 week delay of virus accumulation by comparison with control plants. After reaching the peak at 14 days post-inoculation the virus titre in line 232 decreased with respect to control plants, until the end of the experiment. These results are compatible with the presence of an RNA-mediated resistance mechanism.  |l eng 
593 |a Inst. Invest. Ing. Genet. Biol. M., Vuelta de Obligado 2490, 1428, Buenos Aires, Argentina 
593 |a Estac. Exp. Agropecuaria INTA-L., Mendoza, Argentina 
593 |a Cold Spring Harbor Laboratory, PO Box 100, Cold Spring Harbor, NY 11724, United States 
700 1 |a Bravo-Almonacid, F.F. 
700 1 |a Lampasona, S. 
700 1 |a Rodríguez, F. 
700 1 |a Gracia, O. 
700 1 |a Mentaberry, A.N. 
773 0 |d Blackwell Verlag GmbH Berlin, 1998  |g v. 146  |h pp. 315-319  |k n. 7  |p J. Phytopathol.  |x 09311785  |w (AR-BaUEN)CENRE-5755  |t Journal of Phytopathology 
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