Pregnenolone and progesterone metabolism by the testes of Bufo arenarum
Sliced testis tissue from Bufo arenarum was incubated in the presence of [3H]pregnenolone. Testis fragments were also used for double isotope experiments using [3H]pregnenolone and [14C]progesterone. Specific activities were equated with the addition of radioinert pregnenolone. When yields of radiom...
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Springer Verlag
1998
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| LEADER | 11426caa a22008657a 4500 | ||
|---|---|---|---|
| 001 | PAPER-19904 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518205117.0 | ||
| 008 | 190411s1998 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0032189312 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a JCPBD | ||
| 100 | 1 | |a Canosa, L.F. | |
| 245 | 1 | 0 | |a Pregnenolone and progesterone metabolism by the testes of Bufo arenarum |
| 260 | |b Springer Verlag |c 1998 | ||
| 270 | 1 | 0 | |m Canosa, L.F.; Lab. de Endocrinologia Comparada, Departamento de Ciencias Biologicas, Facultad Ciencias Exactas/Naturales, 1428 Buenos Aires, Argentina; email: fcanosa@abiolo.bg.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Kime, D.E., Hews, E.A., Androgen biosynthesis in vitro by testes from Amphibia (1978) Gen Comp Endocrinol, 35, pp. 280-288 | ||
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| 504 | |a Pozzi, A.G., Lantos, C.P., Ceballos, N.R., Subcellular localization of 3β hydroxysteroid dehydrogenase isomerase in testis of Bufo arenarum H (1997) Gen Comp Endocrinol, 106, pp. 400-406 | ||
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| 504 | |a Slaunwhite, W.R., Sammuels, L.T., Progesterone as a precursor of testicular androgens (1956) J Biol Chem, 220, pp. 341-352 | ||
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| 504 | |a Tanabe, Y., Yano, T., Nakamura, T., Steroid hormone synthesis and secretion by testes, ovary and adrenals of embryonic and postembryonic ducks (1983) Gen Comp Endocrinol, 49, pp. 144-153 | ||
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| 504 | |a Yeung, W.S., Chen, H., Chan, S.T., The in vitro metabolism of radioactive androstenedione and testosterone by the gonads of the protogynous Monopterus albus at different sexual phases: A time-course and seasonal study (1993) Gen Comp Endocrinol, 89, pp. 313-322 | ||
| 504 | |a Wada, M., Wingfield, J.C., Gorbman, A., Correlation between blood levels of androgen and sexual behaviour in male leopard frog Rana pipiens (1976) Gen Comp Endocrinol, 29, pp. 72-77 | ||
| 504 | |a White, R.B., Thomas, P., Adrenal-kidney and gonadal steroidogenesis during sexual differentiation of a reptile with temperature-dependent sex determination (1992) Gen Comp Endocrinol, 88, pp. 10-19 | ||
| 504 | |a Wilson, J.D., Gloyna, R.E., The intranuclear metabolism of testosterone in the accessory organs of reproduction (1970) Recent Progr Horm Res, 26, pp. 309-336 | ||
| 504 | |a Wright, R.S., A reagent for the non-destructive location of steroids and some other lipophilic materials on silica gel thin layer chromatograms (1971) J Chromatogr, 59, pp. 220-221 | ||
| 520 | 3 | |a Sliced testis tissue from Bufo arenarum was incubated in the presence of [3H]pregnenolone. Testis fragments were also used for double isotope experiments using [3H]pregnenolone and [14C]progesterone. Specific activities were equated with the addition of radioinert pregnenolone. When yields of radiometabolites were analysed, pregnenolone was found to be a good precursor for C19 steroids such as dehydroepiandrosterone, 5-androsten-3β,17β diol, testosterone, 5α-dihydrotestosterone and a C21 steroid, 5α-pregnan-3,20 dione. Progesterone mainly converts to 5α-pregnan-3,20 dione, asteroid with unknown function in amphibians. The 5-ene pathway, including 5-androsten-3β,17β diol as intermediate, could be predominant for androgen biosynthesis. Testes bypass not only progesterone but also androstenedione for testosterone biosynthesis. |l eng | |
| 536 | |a Detalles de la financiación: Acknowledgements This work was supported by grants from PRHOM-CONICET and the University of Buenos Aires. The authors thank Dr. Carlos P. Lantos for the critical revision of the manuscript and for his assistance with recrystallisation technique. The authors also thank Mr. Rubén Di Paola for technical assistance. The experiments comply with the ‘‘Principles of animal care’’, publication no. 86-23, revised 1985 of the National Institute of Health and with the Argentine laws. | ||
| 593 | |a Lab. de Endocrinol. Comparada, Depto. de Ciencias Biológicas, Ciudad Universitaria, Pabellón 2, 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a ANURA |
| 690 | 1 | 0 | |a STEROID HORMONES |
| 690 | 1 | 0 | |a STEROIDOGENESIS |
| 690 | 1 | 0 | |a TESTES |
| 690 | 1 | 0 | |a TOAD |
| 690 | 1 | 0 | |a BUFO ARENARUM |
| 700 | 1 | |a Pozzi, A.G. | |
| 700 | 1 | |a Ceballos, N.R. | |
| 773 | 0 | |d Springer Verlag, 1998 |g v. 168 |h pp. 491-496 |k n. 7 |p J. Comp. Physiol. B Biochem. Syst. Environ. Physiol. |x 01741578 |w (AR-BaUEN)CENRE-234 |t Journal of Comparative Physiology - B Biochemical, Systemic, and Environmental Physiology | |
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| 856 | 4 | 0 | |u https://doi.org/10.1007/s003600050169 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_01741578_v168_n7_p491_Canosa |y Handle |
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