Ontogenic and sexual differences in pituitary GnRH receptors and intracellular Ca 2+ mobilization induced by GnRH

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The present experiments were designed in order to elucidate the participation of the developing hypophysis in determining the changing sensitivity of gonadotrophins to gonadotropin-releasing hormone (GnRH) during ontogeny in the rat. To that end, we chose two well defined developmental ages that dif...

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Detalles Bibliográficos
Autor principal: Lacau-Mengido, I.M
Otros Autores: González Iglesias, A., Lux-Lantos, V., Libertun, C., Becú-Villalobos, D.
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1998
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central Dr. Luis F. Leloir (FCEN) de Universidad de Buenos Aires
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024 7 |2 scopus  |a 2-s2.0-7144228632 
024 7 |2 cas  |a buserelin, 57982-77-1; calcium ion, 14127-61-8; gonadorelin, 33515-09-2, 9034-40-6; gonadotropin, 63231-54-9; Buserelin, 57982-77-1; Calcium, 7440-70-2; Gonadotropin-Releasing Hormone, 33515-09-2; Iodine Radioisotopes; Receptors, LHRH 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a EOCRE 
100 1 |a Lacau-Mengido, I.M. 
245 1 0 |a Ontogenic and sexual differences in pituitary GnRH receptors and intracellular Ca 2+ mobilization induced by GnRH 
260 |c 1998 
270 1 0 |m Libertun, C.Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina; email: libertun@proteus.dna.uba.ar 
506 |2 openaire  |e Política editorial 
504 |a Becu-Villalobos, D., Lacau-Mengido, I.M., Libertun, C., (1990) Cell. Mol. Neurobiol., 10, pp. 473-484 
504 |a Ojeda, S.R., Urbanski, H.F., (1994) The Physiology of Reproduction, pp. 363-409. , Knobil, E. and Neill, J. (eds.) Raven, New York 
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504 |a Ojeda, S.R., Jameson, H.E., Mc Cann, S.M., (1977) Endocrinology, 100, pp. 440-451 
504 |a Debeljuk, L., Arimura, A., Schally, A.V., (1972) Endocrinology, 90, pp. 585-589 
504 |a Aubert, M.L., Begeot, M., Winiger, B.P., Morel, G., Sizonenko, P.C., Dubois, P.M., (1985) Endocrinology, 116, pp. 1565-1576 
504 |a Hompes, P.G.A., Vermes, I., Tilders, F.J.H., Schoemaker, J., (1982) Neuroendocrinology, 35, pp. 8-12 
504 |a Jakubowski, M., Blum, M., Roberts, J.L., (1991) Endocrinology, 128, pp. 2702-2708 
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504 |a Harter, D.E., Ramirez, V.D., (1985) Neuroendocrinology, 40, pp. 476-482 
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504 |a Chan, V., Clayton, R., Knox, G., Catt, K.J., (1981) Endocrinology, 108, pp. 2086-2092 
504 |a Dorner, G., (1981) Vitam. Horm., 38, pp. 325-381 
504 |a Stojilkovic, S.S., Reinhart, J., Catt, K.J., (1994) Endocr. Rev., 15, pp. 462-499 
504 |a Ortmann, O., Stojilkovic, S.S., Cesnjaj, M., Emons, G., Catt, K.J., (1992) Endocrinology, 131, pp. 1565-1568 
504 |a Ortmann, O., Merelli, F., Stojilkovic, S.S., Schultz, K.D., Emons, G., Catt, K.J., (1994) J. Steroid Biochem. Mol. Biol., 48, pp. 47-54 
504 |a Debeljuk, L., Arimura, A., Schally, A.V., (1972) Endocrinology, 90, p. 1499 
504 |a Naish, S.J., Brawer, J., Robaire, B., Ruf, K.B., (1986) Biol. Reprod., 34, pp. 549-557 
504 |a Duncan, J.A., Dalkin, A.C., Barkan, A., Regiani, S., Marshall, J.C., (1983) Endocrinology, 113, pp. 2238-12236 
504 |a Fink, G., Henderson, S.R., (1977) J. Endocrinol., 73, pp. 157-164 
504 |a Barraclough, C.A., Turgeon, J.L., (1974) Endocrinologie Sexuelle de la Periode Perinatale, pp. 339-357. , Forest, M.G. and Bertrand, J. (eds.) INSERM, Paris 
504 |a Chiappa, S.A., Fink, G., (1977) J. Endocrinol., 73, pp. 157-164 
504 |a Chen, H.T., (1988) Mol. Cell Endocrinol., 57, pp. 33-39 
504 |a Tomic, M., Cesnjaj, M., Catt, K.J., Stojilkovic, S.S., (1994) Endocrinology, 135, pp. 1762-1771 
504 |a Nishizaki, T., Walent, J.H., Kowalchyk, J.A., Martin, T.F.J., (1992) J. Biol. Chem., 267, pp. 23972-23981 
504 |a Stojilkovic, S.S., Stutzin, A., Izumi, S.-I., Dufour, S., Torsello, A., Virmani, (1990) New Biol., 3, pp. 272-283 
504 |a Chamson-Reig, A., Lux-Lantos, V., Tesone, M., Libertun, C., (1997) Endocrine, 6, pp. 1-7 
504 |a Attardi, B., Happe, H.K., (1986) Endocrinology, 119, pp. 274-283 
504 |a Anderson, J.M., Yasumoto, T., Cronin, M.J., (1987) Life Sci., 103, pp. 249-257 
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504 |a Grynkiewicz, G., Poenie, M., Tsien, R.Y., (1985) J. Biol. Chem., 260, pp. 3440-3450 
520 3 |a The present experiments were designed in order to elucidate the participation of the developing hypophysis in determining the changing sensitivity of gonadotrophins to gonadotropin-releasing hormone (GnRH) during ontogeny in the rat. To that end, we chose two well defined developmental ages that differ markedly in sexual and ontogenic characteristics of hypophyseal sensitivity to GnRH, 15 and 30 d. In order to study sex differences and the role of early sexual organization of the hypothalamus, experiments were carried out in males, females, and neonatally androgenized females (TP females). We evaluated (1) the characteristics of pituitary GnRH receptors, and (2) associated changes in GnRH-induced mobilization of intracellular Ca 2+ (a second messenger involved in gonadotropins exocytosis). We measured binding characteristics of the GnRH analog D- Ser(TBu)6-des-Gly10-GnRH ethylamide in pituitary homogenates. We found that K(ds) did not vary among the different sex groups. Total number and concentration of receptors decreased in the female rat from 15-30 d of age, whereas in the male and TP female, receptors/pituitary increased, and the concentration/mg tissue did not change. Also, at 30 days of age, males presented higher content and concentration of receptors than females, and higher content than TP females. In order to evaluate if developmental and sexual differences in pituitary sensitivity to GnRH might be expressed through variations in the intracellular Ca 2+ signal, we studied the mobilization of intracellular Ca 2+ induced by GnRH (1 x 10 -8 to 1 x 10 - 11 M) in a suspension of dispersed pituitary cells in the six groups. In cells from 15-d-old females, Ca 2+ response was greater than in 30-d-old females at the doses of 10 -8 to 10 -10 M, indicating that in the infantile female rat activation of highly concentrated GnRH receptors is reflected in an increase in signal transduction mediated by Ca 2+. In males and in female rats androgenized at birth, there was also a decrease in the magnitude of intracellular Ca 2+ mobilization induced by GnRH (10 -8 to 10 -1 0 M) from 15-30 d of age, even though the concentration of GnRH receptors did not change in the same period. In conclusion, the present results suggest that high sensitivity to GnRH, which has been described in the female infantile rat, may be related to elevated concentration of hypophyseal receptors coupled to an increase of intracellular calcium response to GnRH, both parameters decreasing as the rat matures. In males, the greater sensitivity that has been described for GnRH at 30 d in comparison to 15 d is paralleled by an increase in the total number of GnRH receptors per pituitary (and not in their concentration), but not in an increase in the magnitude of Ca 2+ mobilization induced by GnRH. On the other hand, neonatal sexual organization of the hypothalamus is involved in the differential expression of GnRH receptors, but does not modulate mobilization of intracellular Ca 2+ induced by the decapeptide.  |l eng 
536 |a Detalles de la financiación: Universidad de Buenos Aires 
536 |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas 
536 |a Detalles de la financiación: Universidad de Buenos Aires 
536 |a Detalles de la financiación: This work was supported by Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina and Fundación Antorchas and Universidad de Buenos Aires (UBA). 
593 |a Inst. de Biol. y Med. Experimental, CONICET, UBA. V. de Obligado 2490, Buenos Aires, Argentina 
593 |a Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina 
690 1 0 |a CALCIUM 
690 1 0 |a GNRH RECEPTORS 
690 1 0 |a ONTOGENY 
690 1 0 |a PITUITARY 
690 1 0 |a SEXUAL DIFFERENCES 
690 1 0 |a BUSERELIN 
690 1 0 |a CALCIUM ION 
690 1 0 |a GONADORELIN 
690 1 0 |a GONADORELIN DERIVATIVE 
690 1 0 |a GONADORELIN RECEPTOR 
690 1 0 |a GONADOTROPIN 
690 1 0 |a ANDROGEN THERAPY 
690 1 0 |a ANIMAL CELL 
690 1 0 |a ANIMAL EXPERIMENT 
690 1 0 |a ANIMAL TISSUE 
690 1 0 |a ARTICLE 
690 1 0 |a CALCIUM MOBILIZATION 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a DISSOCIATION CONSTANT 
690 1 0 |a FEMALE 
690 1 0 |a GENE EXPRESSION 
690 1 0 |a HORMONE BINDING 
690 1 0 |a HORMONE SENSITIVITY 
690 1 0 |a HYPOPHYSIS 
690 1 0 |a HYPOTHALAMUS 
690 1 0 |a MALE 
690 1 0 |a NEWBORN 
690 1 0 |a NONHUMAN 
690 1 0 |a ONTOGENY 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a RAT 
690 1 0 |a SECOND MESSENGER 
690 1 0 |a SEX DIFFERENCE 
690 1 0 |a SIGNAL TRANSDUCTION 
690 1 0 |a AGING 
690 1 0 |a ANIMALS 
690 1 0 |a BUSERELIN 
690 1 0 |a CALCIUM 
690 1 0 |a FEMALE 
690 1 0 |a GONADOTROPIN-RELEASING HORMONE 
690 1 0 |a IODINE RADIOISOTOPES 
690 1 0 |a MALE 
690 1 0 |a PITUITARY GLAND, ANTERIOR 
690 1 0 |a RATS 
690 1 0 |a RATS, SPRAGUE-DAWLEY 
690 1 0 |a RECEPTORS, LHRH 
690 1 0 |a SEX CHARACTERISTICS 
700 1 |a González Iglesias, A. 
700 1 |a Lux-Lantos, V. 
700 1 |a Libertun, C. 
700 1 |a Becú-Villalobos, D. 
773 0 |d 1998  |g v. 8  |h pp. 177-183  |k n. 2  |p Endocrine  |x 1355008X  |t Endocrine 
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856 4 0 |u https://doi.org/10.1385/ENDO:8:2:177  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_1355008X_v8_n2_p177_LacauMengido  |y Handle 
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