Rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system: Application to the determination of glucose in serum samples
A recently introduced redox osmium complex of the type [Os(bpy)2Cl(pyX]+, where pyX corresponds to a pyridine derivative bearing an aldehyde group, and glucose oxidase [EC 1.1.3.4] were covalently immobilized on the glassy carbon electrode surface (upper cell body). This approach has been designed i...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
2001
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 06693caa a22010097a 4500 | ||
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| 001 | PAPER-1964 | ||
| 003 | AR-BaUEN | ||
| 005 | 20241007095817.0 | ||
| 008 | 190411s2001 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0035850580 | |
| 030 | |a ACACA | ||
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 100 | 1 | |a Lapierre, A.V. | |
| 245 | 1 | 0 | |a Rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system: Application to the determination of glucose in serum samples |
| 260 | |c 2001 | ||
| 270 | 1 | 0 | |m Raba, J.; Department of Analytical Chemistry, National University of San Luis, Chacabuco y Pedernera, 5700 San Luis, Argentina; email: jraba@unsl.edu.ar |
| 504 | |a Schuhmann, W., (1995) Mikrochim. Acta, 121, pp. 1-29 | ||
| 504 | |a Ghindilis, A.L., Atasanov, P., Wilkins, E., (1997) Electroanalysis, 9, pp. 661-674 | ||
| 504 | |a Raba, J., Mottola, H.A., (1995) Crit. Rev. Anal. Chem., 25 (1), pp. 1-42 | ||
| 504 | |a Bartlett, P.N., Tebbut, P., Whitaker, R.P., (1991) Progr. React. Kinet., 16, pp. 55-65 | ||
| 504 | |a Albery, W.J., Bartlett, N.P., Cass, A.E.G., Craston, D.H., Haggett, B.G.D., (1986) J. Chem. Soc., Faraday Trans., 82, pp. 1033-1058 | ||
| 504 | |a Matsumoto, K., Baeza Baeza, J.J., Mottola, H.A., (1993) Anal. Chem., 65, pp. 636-639 | ||
| 504 | |a Danilowicz, C., Cortón, E., Battaglini, F., (1998) J. Electroanal. Chem., 445, pp. 89-94 | ||
| 504 | |a Heller, A., (1992) J. Phys. Chem., 96, pp. 3579-3587 | ||
| 504 | |a Degani, Y., Heller, A., (1987) J. Phys. Chem., 91, pp. 1285-1289 | ||
| 504 | |a Degani, Y., Heller, A., (1988) J. Am. Chem. Soc., 110, pp. 2615-2620 | ||
| 504 | |a Heller, A., Degani, Y., (1988) Redox Chemistry and Interfacial Behavior of Biological Molecules, pp. 151-171. , G. Dryhurst, K. Niki (Eds.), Plenum Press, New York | ||
| 504 | |a Lapierre, A.V., Olsina, R.A., Raba, J., (1998) Anal. Chem., 70, pp. 3679-3684 | ||
| 504 | |a Lapierre, A.V., Olsina, R.A., Raba, J., (1999) Anal. Chim. Acta, 396, pp. 143-149 | ||
| 504 | |a Opekar, F., Beran, P., (1976) J. Electroanal. Chem. Interf. Electrochem., 69, pp. 1-105 | ||
| 504 | |a Kamin, R.A., Wilson, G., (1980) Anal. Chem., 52, pp. 1198-1205 | ||
| 504 | |a Wang, J., Lin, M.S., (1998) Anal. Chem., 218, pp. 281-290 | ||
| 504 | |a Richter, P., López Ruiz, B., Sánchez-Cabezudo, M., Mottola, H.A., (1996) Anal. Chem., 68, pp. 1701-1705 | ||
| 504 | |a Briggs, E.G., Haldane, J.B.S., (1925) Biochem. J., 19, pp. 338-339 | ||
| 504 | |a Alcock, S.J., Turner, A.P.F., (1994) IEEE Engineering in Medicine and Biology, pp. 319-325. , June/July | ||
| 506 | |2 openaire |e Política editorial | ||
| 520 | 3 | |a A recently introduced redox osmium complex of the type [Os(bpy)2Cl(pyX]+, where pyX corresponds to a pyridine derivative bearing an aldehyde group, and glucose oxidase [EC 1.1.3.4] were covalently immobilized on the glassy carbon electrode surface (upper cell body). This approach has been designed in a sealed cell to be used under continuous-flow/stopped-flow/continuous-flow processing. The operating characteristic of the integrated bioreactor/detector unit has been utilized in the determination of glucose in serum samples using the electroactive polymer as mediator in the re-oxidation of GOx and amperometric monitoring. The determinations are based on the rate of response under stopped-flow conditions. The trend in values of the apparent Michaelis-Menten constant confirms the validity of the approach to sensitive determination utilizing very small amounts of biocatalysts. The determination of glucose was possible with a limit of detection of 0.020 mM in the processing of as many as 30 samples per hour. © 2001 Elsevier Science B.V. All rights reserved. |l eng | |
| 536 | |a Detalles de la financiación: International Foundation for Science | ||
| 536 | |a Detalles de la financiación: Universidad Nacional de San Luis | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: The authors wish to thank the financial support from the Universidad Nacional de San Luis and the Consejo Nacional de Investigaciones Cientı́ficas y Técnicas (CONICET). One of the authors (A.V.L.) acknowledges support in the form of a fellowship from the Universidad Nacional de San Luis, Argentina. F.B. acknowledges financial support from International Foundation of Science. Dr. Monica Rivera and Dr. Daniel Decena are gratefully acknowledged for providing the human blood samples. | ||
| 593 | |a Department of Analytical Chemistry, National University of San Luis, Chacabuco y Pedernera, 5700 San Luis, Argentina | ||
| 593 | |a INQUIMAE, University of Buenos Aires, Pabellón 2, 1428 Buenos Aires, Argentina | ||
| 650 | 1 | 7 | |2 spines |a CARBON |
| 650 | 1 | 7 | |2 spines |a PH |
| 690 | 1 | 0 | |a BIOREACTOR |
| 690 | 1 | 0 | |a BIOSENSORS |
| 690 | 1 | 0 | |a FLOW SYSTEM |
| 690 | 1 | 0 | |a OSMIUM |
| 690 | 1 | 0 | |a ALDEHYDE |
| 690 | 1 | 0 | |a BLOOD EXTRACT |
| 690 | 1 | 0 | |a DIANISIDINE |
| 690 | 1 | 0 | |a FUNCTIONAL GROUP |
| 690 | 1 | 0 | |a GLUCOSE |
| 690 | 1 | 0 | |a GLUCOSE OXIDASE |
| 690 | 1 | 0 | |a OSMIUM DERIVATIVE |
| 690 | 1 | 0 | |a POLYMER |
| 690 | 1 | 0 | |a PYRIDINE DERIVATIVE |
| 690 | 1 | 0 | |a AMPEROMETRY |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a BIOCATALYST |
| 690 | 1 | 0 | |a BIOREACTOR |
| 690 | 1 | 0 | |a BLOOD SAMPLING |
| 690 | 1 | 0 | |a CARBOHYDRATE ANALYSIS |
| 690 | 1 | 0 | |a CELL VOLUME |
| 690 | 1 | 0 | |a COMPARATIVE STUDY |
| 690 | 1 | 0 | |a ELECTRODE |
| 690 | 1 | 0 | |a ELECTRON TRANSPORT |
| 690 | 1 | 0 | |a FLOW MEASUREMENT |
| 690 | 1 | 0 | |a GLUCOSE ASSAY |
| 690 | 1 | 0 | |a GLUCOSE BLOOD LEVEL |
| 690 | 1 | 0 | |a MICHAELIS CONSTANT |
| 690 | 1 | 0 | |a OXIDATION |
| 690 | 1 | 0 | |a OXIDATION REDUCTION REACTION |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a REACTION TIME |
| 690 | 1 | 0 | |a VALIDATION PROCESS |
| 700 | 1 | |a Battaglini, Fernando | |
| 700 | 1 | |a Raba, J. | |
| 773 | 0 | |d 2001 |g v. 443 |h pp. 17-24 |k n. 1 |p Anal. Chim. Acta |x 00032670 |w (AR-BaUEN)CENRE-133 |t Analytica Chimica Acta | |
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| 856 | 4 | 0 | |u https://doi.org/10.1016/S0003-2670(01)01186-2 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00032670_v443_n1_p17_Lapierre |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032670_v443_n1_p17_Lapierre |y Registro en la Biblioteca Digital |
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| 999 | |c 62917 | ||