Effects of different steroid-biosynthesis inhibitors on the testicular steroidogenesis of the toad Bufo arenarum
Testis fragments from Bufo arenarum were incubated with [7(n)-3H]pregnenolone (P5), [1,2-3H]de-hydroepiandrosterone (DHEA) and [1,2,6,7-3H]testosterone (T), and different steroid-biosynthesis inhibitors. The inhibitors used were: cyanoketone (CNK), spironolactone (SPNL) and finasteride (FIN). CNK si...
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Springer Verlag
2001
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| LEADER | 10431caa a22007457a 4500 | ||
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| 003 | AR-BaUEN | ||
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| 008 | 190411s2001 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0034867294 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a JCPBD | ||
| 100 | 1 | |a Canosa, L.F. | |
| 245 | 1 | 0 | |a Effects of different steroid-biosynthesis inhibitors on the testicular steroidogenesis of the toad Bufo arenarum |
| 260 | |b Springer Verlag |c 2001 | ||
| 270 | 1 | 0 | |m Ceballos, N.R.; Departamento de Ciencias Biologicas, Facultad de Ciencias Exactas, Ciudad Universitaria, 1428 Buenos Aires, Argentina; email: nceballo@bg.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Canosa, L.F., Pozzi, A.G., Ceballos, N.R., Pregnenolone and progesterone metabolism by testes of Bufo arenarum (1998) J Comp Physiol B, 168, pp. 491-496 | ||
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| 504 | |a Ellis, L.C., Berlinger, D.L., Sequential biotransformation of ΔD5 -pregnenolone-7α-3H and progesterone-4-14 C into androgens by mouse testes (1965) Endocrinology, 76, pp. 591-599 | ||
| 504 | |a Hall, P.F., Testicular steroid synthesis: Organization and regulation (1994), 1, pp. 1335-1443. , Knobil E, Neill J (eds) The physiology of reproduction. Raven, New York; Hall, P.F., Sozer, C.C., Eik-Nes, K.B., Formation of dehydro-epiandrosterone during in vivo and in vitro biosynthesis of testosterone by testicular tissue (1964) Endocrinology, 74, pp. 35-43 | ||
| 504 | |a Hirsch, K.S., Jones, C.D., Audia, J.E., Andersson, S., McQuaid, L., Stamm, N.B., Neubauer, B.L., Russell, D.W., LY191704: A selective, nonsteroidal inhibitor of human steroid 5-reductase type I (1993) Proc Natl Acad Sci USA, 90, pp. 5277-5281 | ||
| 504 | |a Hoschoian, J.C., Brownie, A.C., Pathway of androgen synthesis in monkey testis (1967) Steroids, 10, pp. 49-69 | ||
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| 504 | |a Lisboa, B.P., Thin-layer chromatography of steroid, sterols and related compounds (1969), 15, pp. 3-157. , Clayton RB (ed) Methods in enzymology. Academic Press, New York; Menard, R.H., Martin, H.F., Stripp, B., Gillette, J.R., Bartter, F.C., Spironolactone and cytochrome P-450: Impairment of steroid hydroxylation in the adrenal cortex (1974) Life Sci, 15, pp. 1639-1648 | ||
| 504 | |a O'Donnell, L., Stanton, P.G., Wreford, N.G., Robertson, D.M., McLachlan, R.I., Inhibition of 5 alpha-reductase activity impairs the testosterone-dependent restoration of spermiogenesis in adult rats (1996) Endocrinology, 137, pp. 2703-2710 | ||
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| 504 | |a Penning, T.M., Molecular endocrinology of hydroxysteroid dehydrogenases (1997) Endocr Rev, 18, pp. 281-305 | ||
| 504 | |a Pozzi, A.G., Lantos, C.P., Ceballos, N.R., Mitochondrial localization of 3 beta-hydroxysteroid dehydrogenase 5-ene isomerase in interrenals of the toad Bufo arenarum H (1996) Gen Comp Endocrinol, 103, pp. 176-181 | ||
| 504 | |a Pozzi, A.G., Lantos, C.P., Ceballos, N.R., Subcellular localization of 3 beta hydroxysteroid dehydrogenase isomerase in testis of Bufo arenarum H (1997) Gen Comp Endocrinol, 106, pp. 400-406 | ||
| 504 | |a Preslock, J.P., Steroidogenesis in the mammalian testis (1980) Endocr Rev, 1, pp. 132-139 | ||
| 504 | |a Preslock, J.P., Steinberger, E., Pathway of testosterone biosynthesis in the testis of the marmoset Saguinus oedipus (1976) Steroids, 28, pp. 775-784 | ||
| 504 | |a Preslock, J.P., Steinberger, E., Androgen biosynthesis by marmoset testes in vitro (1977) Gen Comp Endocrinol, 31, pp. 101-105 | ||
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| 504 | |a Takahashi, M., Luu-The, V., Labrie, F., Inhibitory effect of synthetic progestins, 4-MA and cyanoketone on human placental 3 beta-hydroxysteroid dehydrogenase/5-4-ene- isomerase activity (1990) J Steroid Biochem Mol Biol, 37, pp. 231-236 | ||
| 504 | |a Wright, R.S., A reagent for the non-destructive location of steroids and some other lipophilic materials on silica gel thin layer chromatograms (1971) J Chromatogr, 59, pp. 220-221 | ||
| 520 | 3 | |a Testis fragments from Bufo arenarum were incubated with [7(n)-3H]pregnenolone (P5), [1,2-3H]de-hydroepiandrosterone (DHEA) and [1,2,6,7-3H]testosterone (T), and different steroid-biosynthesis inhibitors. The inhibitors used were: cyanoketone (CNK), spironolactone (SPNL) and finasteride (FIN). CNK significantly increased the recovery of 3β-hydroxy-5-ene steroids while SPNL reduced the metabolism of P5 and the production of C19-steroids. The metabolism of C19-substrates was only modified by CNK, which reduced the transformation of DHEA without modifying the metabolism of T. To determine the degree of inhibition exerted by the inhibitors used, the activities of the enzymes were estimated as the percentage of their contribution to the total steroid metabolism. CNK strongly inhibited the activity of hydroxysteroid dehydrogenase/isomerase if its contribution was estimated using both P5 and DHEA. If the analysis was made considering both activities associated to cytochrome P450 17α-hydroxylase, C17-20 lyase (P450c17), it became evident that SPNL inhibited both of them. The percent contribution of 17β-hydroxysteroid dehydrogenase (17βHSD) activity diminished in the presence of CNK only if it was estimated considering P5 and DHEA metabolism. SPNL produced a significant inhibition of 17βHSD when its contribution was estimated considering P5 metabolism. However, SPNL was insufficient if DHEA or T were considered. The effect of SPNL on the contribution of 17βHSD could be due to the reduction of C19-substrates. The activity of 5α-reductase was inhibited by CNK only if results from P5 and DHEA were considered. |l eng | |
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires | ||
| 536 | |a Detalles de la financiación: National Council for Scientific Research | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: Acknowledgments This work was supported by grants from the National Research Council of Argentina (CONICET) to its Prog-rama de Regulación Hormonal y Metabólica (PRHOM), and the University of Buenos Aires. The authors wish to thank Dr. Carlos P. Lantos for the critical revision of the manuscript and Mr. Rubén Di Paola for technical assistance. The experiments comply with de ``Principles of animal care'', publication No. 86-23, revised 1985 of the National Institute of Health and also with the argentine laws. | ||
| 593 | |a Programa de Regulación Hormonal y Metabólica (PRHOM-CONICET), Departamento de Ciencias Biológicas, Universidad de Buenos Aires, Buenos Aires, Argentina | ||
| 593 | |a Laboratorio de Endocrinología Comparada, Departamento de Ciencias Biológicas, Pabellón 2. Ciudad Universitaria, 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a AMPHIBIA |
| 690 | 1 | 0 | |a ANURA |
| 690 | 1 | 0 | |a ENZYME-INHIBITION |
| 690 | 1 | 0 | |a STEROID-HORMONES |
| 690 | 1 | 0 | |a TESTES |
| 700 | 1 | |a Ceballos, N.R. | |
| 773 | 0 | |d Springer Verlag, 2001 |g v. 171 |h pp. 519-526 |k n. 6 |p J. Comp. Physiol. B Biochem. Syst. Environ. Physiol. |x 01741578 |w (AR-BaUEN)CENRE-234 |t Journal of Comparative Physiology - B Biochemical, Systemic, and Environmental Physiology | |
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