Differential expression of the members of the Asr gene family in tomato (Lycopersicon esculentum)

In this work, we continued to dissect the Asr (ABA/water stress/ripening-induced) gene family originally described in tomato. A RT-PCR-based strategy was developed to assess the organ (leaf, root and fruit) and developmental (immature and ripe fruit) specificity of expression of the three known memb...

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Autor principal: Maskin, L.
Otros Autores: Gudesblat, G.E, Moreno, J.E, Carrari, F.O, Frankel, N., Sambade, A., Rossi, M., Iusem, N.D
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2001
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Acceso en línea:Registro en Scopus
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100 1 |a Maskin, L. 
245 1 0 |a Differential expression of the members of the Asr gene family in tomato (Lycopersicon esculentum) 
260 |c 2001 
506 |2 openaire  |e Política editorial 
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520 3 |a In this work, we continued to dissect the Asr (ABA/water stress/ripening-induced) gene family originally described in tomato. A RT-PCR-based strategy was developed to assess the organ (leaf, root and fruit) and developmental (immature and ripe fruit) specificity of expression of the three known members of the Asr gene family under normal and stress conditions. Our results allow us to conclude that whereas Asr1 and Asr2 are the members of the family preferentially induced by desiccation in leaves, Asr2 is the only one activated in the roots from water-deficit-stressed plants. We also observed that expression of the three genes does not change significantly in fruit at different developmental stages, except for that of Asr2, which decreases after the breaker yellow stage. In addition, we identified a 72-amino acid polar peptide region, rich in His, Lys, Glu and Ala, which contains two internal imperfect repeats and is highly conserved in more recently discovered Asr-like proteins from other plant species exposed to different kinds of abiotic stress such as water deficit, salt, cold and/or limiting amount of light. © 2001 Elsevier Science Ireland Ltd. All rights reserved.  |l eng 
536 |a Detalles de la financiación: Universidad de Buenos Aires 
536 |a Detalles de la financiación: Agencia Nacional de Promoción Científica y Tecnológica 
536 |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas 
536 |a Detalles de la financiación: This work was funded by University of Buenos Aires (UBA), Argentina, Agencia Nacional para la Promoción Cientı́fica y Tecnológica (ANPCyT), Argentina and Consejo Nacional de Investigaciones Cientı́ficas y Técnológicas (CONICET), Argentina. Fernando O. Carrari, Laura Maskin and Gustavo E. Gudesblat hold fellowships from UBA, CONICET and Fundación Antorchas, Argentina, respectively. 
593 |a Laboratorio de Fisiología y Biología Molecular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon 2, Ciudad Universitaria, (1428) Buenos Aires, Argentina 
690 1 0 |a ABA 
690 1 0 |a ASR GENES 
690 1 0 |a FRUIT RIPENING 
690 1 0 |a LYCOPERSICON ESCULENTUM 
690 1 0 |a WATER STRESS 
690 1 0 |a AMINO ACIDS 
690 1 0 |a PLANTS (BOTANY) 
690 1 0 |a PROTEINS 
690 1 0 |a POLAR PEPTIDE REGION 
690 1 0 |a ALANINE 
690 1 0 |a GENE PRODUCT 
690 1 0 |a GLUTAMIC ACID 
690 1 0 |a HISTIDINE 
690 1 0 |a LYSINE 
690 1 0 |a PROTEIN ASR 
690 1 0 |a UNCLASSIFIED DRUG 
690 1 0 |a AMINO ACID SEQUENCE 
690 1 0 |a ARTICLE 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a DESICCATION 
690 1 0 |a FRUIT 
690 1 0 |a GENE EXPRESSION 
690 1 0 |a GENETIC CONSERVATION 
690 1 0 |a MULTIGENE FAMILY 
690 1 0 |a NONHUMAN 
690 1 0 |a PLANT GROWTH 
690 1 0 |a PLANT LEAF 
690 1 0 |a PLANT ROOT 
690 1 0 |a REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION 
690 1 0 |a STRESS 
690 1 0 |a TOMATO 
650 1 7 |2 spines  |a GENES 
700 1 |a Gudesblat, G.E. 
700 1 |a Moreno, J.E. 
700 1 |a Carrari, F.O. 
700 1 |a Frankel, N. 
700 1 |a Sambade, A. 
700 1 |a Rossi, M. 
700 1 |a Iusem, N.D. 
773 0 |d 2001  |g v. 161  |h pp. 739-746  |k n. 4  |p Plant Sci.  |x 01689452  |w (AR-BaUEN)CENRE-6512  |t Plant Science 
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