Genomic in situ hybridization (GISH) of Tripsacum dactyloides and Zea mays ssp. mays with B chromosomes

Genomic affinities between Tripsacum dactyloides (2n = 72) and Zea mays ssp. mays (2n = 20 + 5 B) were analyzed through GISH (genomic in situ hybridization) to ascertain the degree of chromosome homology between the two genera. Mitotic cells of T. dactyloides were simultaneously probed with total ge...

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Autor principal: Poggio, L.
Otros Autores: Confalonieri, V., Comas, C., Cuadrado, A., Jouve, N., Naranjo, C.A
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1999
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
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030 |a GENOE 
100 1 |a Poggio, L. 
245 1 0 |a Genomic in situ hybridization (GISH) of Tripsacum dactyloides and Zea mays ssp. mays with B chromosomes 
260 |c 1999 
270 1 0 |m Naranjo, C.A.; Inst. Fitotecnico de Santa Catalina, Universidad Nacional de La Plata, Centro de Investigaciones Geneticas, C.C. 4, 1836 Llavallol, Buenos Aires, Argentina; email: naranjo@bayersistemas.com.ar 
506 |2 openaire  |e Política editorial 
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504 |a Longley, A.E., Supernumerary chromosomes in Zea mays (1927) J. Agric. Res., 35, pp. 769-784 
504 |a Longley, A.E., Chromosomes of maize from North American Indians (1938) J. Agric. Res., 56, pp. 177-195 
504 |a Maguire, P.M., Divergence in Tripsacum and Zea chromosomes (1961) Evolution, 15, pp. 394-400 
504 |a Mangelsdorf, P.C., Reeves, R.G., Hybridization of maize, Tripsacum and Euchlaena (1931) J. Hered., 22, pp. 329-343 
504 |a Maniatis, T., Fritsch, E.F., Sambrook, J., (1982) Molecular Cloning: A Laboratory Manual, , Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 
504 |a McClintock, B., Kato-Y, T.A., Blumenschein, A., Constitución cromosómica de las razas de maíz (1981) Su Significado en la Interpretación de Relaciones Entre las Razas y Variedades en las Américas, , Colegio de Postgraduados, Chapingo, México 
504 |a Molina, M.C., Naranjo, C.A., Cytogenetic studies in the genus Zea 1. Evidence for five as the basic chromosome number (1987) Theor. Appl. Genet., 73, pp. 542-550 
504 |a Moore, G., Devos, K.M., Wang, Z., Gale, M.D., Cereal genome evolution. Grasses, line up and form a circle (1995) Curr. Biol., 5, pp. 737-739 
504 |a Naranjo, C., Poggio, L., Molina, M., Bernatene, E., Increase in multivalent frequency in F1 hybrids of Zea diploperennis × Z. perennis by colchicine treatment (1994) Hereditas, 120, pp. 241-244 
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504 |a Randolph, L.F., Types of supernumerary chromosomes in maize (1928) Anat. Res., 41, p. 102 
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520 3 |a Genomic affinities between Tripsacum dactyloides (2n = 72) and Zea mays ssp. mays (2n = 20 + 5 B) were analyzed through GISH (genomic in situ hybridization) to ascertain the degree of chromosome homology between the two genera. Mitotic cells of T. dactyloides were simultaneously probed with total genomic DNA from Z. mays ssp. mays (2n = 20) and with rDNA (pTA71). A disperse pattern of hybridization signal among all 72 chromosomes, corresponding to maize total DNA, and six strong fluorescent signals due to the rDNA probe hybridizing on 3 chromosome pairs of T. dactyloides were observed. Mitotic chromosomes from Z. mays ssp. mays (2n = 20 + 5 B) were hybridized with a maize line that lacked B chromosomes and knobs and with total DNA from T. dactyloides. The knobless line of maize hybridized intensely on all chromosomes except for some regions where the probe bound less. Tripsacum dactyloides bound intensely on one terminal region of each B chromosome and to some regions of chromosome pairs 2, 6, and 8. These regions are DAPI positive and coincide with regions that displayed lower affinity with the probe from the knobless maize line. The possible significance of these results is discussed briefly.  |l eng 
593 |a Depto. de Ciencias Biológicas, Facultad de Cie. Exact. y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina 
593 |a Inst. Fitotecnico de Santa Catalina, Fac. de Cie. Agrarias y Forestales, Universidad Nacional de La Plata, C.C. 4, 1836 Llavallol, Buenos Aires, Argentina 
593 |a Depto. de Biologia Cel. y Genetica, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain 
690 1 0 |a GENOMIC IN SITU HYBRIDIZATION 
690 1 0 |a GISH 
690 1 0 |a MAIZE B CHROMOSOMES 
690 1 0 |a TRIPSACUM DACTYLOIDES 
690 1 0 |a ZEA MAYS SSP. MAYS 
690 1 0 |a CHROMOSOMAL LOCALIZATION 
690 1 0 |a DNA PROBE 
690 1 0 |a FLUORESCENCE 
690 1 0 |a GENOME ANALYSIS 
690 1 0 |a GENOMIC IN SITU HYBRIDIZATION 
690 1 0 |a IN SITU HYBRIDIZATION 
690 1 0 |a MITOSIS 
690 1 0 |a RIBOSOMAL DNA 
690 1 0 |a TRIPSACUM DACTYLOIDES 
690 1 0 |a ZEA MAYS 
700 1 |a Confalonieri, V. 
700 1 |a Comas, C. 
700 1 |a Cuadrado, A. 
700 1 |a Jouve, N. 
700 1 |a Naranjo, C.A. 
773 0 |d 1999  |g v. 42  |h pp. 687-691  |k n. 4  |p Genome  |x 08312796  |w (AR-BaUEN)CENRE-592  |t Genome 
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856 4 0 |u https://hdl.handle.net/20.500.12110/paper_08312796_v42_n4_p687_Poggio  |y Handle 
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