Peripheral-type benzodiazepine receptors are highly concentrated in mitochondrial membranes of rat testicular interstitial cells
The binding of 3H-RO 5-4864 to the peripheral-type benzodiazepine receptors (PBZDR) in rat testicular interstitial cells (TIC) was characterized. The binding was saturable, reversible and showed a single high-affinity (K(d) = 5.02 ± 0.86 nM) class of binding sites. The maximal binding capacity (B(ma...
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| Otros Autores: | , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1990
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| Materias: | |
| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 03645caa a22005897a 4500 | ||
|---|---|---|---|
| 001 | PAPER-19405 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518205046.0 | ||
| 008 | 190411s1990 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0025173643 | |
| 024 | 7 | |2 cas |a 4' chlorodiazepam, 14439-61-3; diazepam, 439-14-5; flunitrazepam, 1622-62-4; n sec butyl 1 (2 chlorophenyl) n methyl 3 isoquinolinecarboxamide, 85532-75-8 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a NUNDA | ||
| 100 | 1 | |a Calvo, D.J. | |
| 245 | 1 | 0 | |a Peripheral-type benzodiazepine receptors are highly concentrated in mitochondrial membranes of rat testicular interstitial cells |
| 260 | |c 1990 | ||
| 270 | 1 | 0 | |m Ritta, M.N.; Inst. de Biologia/Med. Exp., Obligado 2490, 1428 Buenos Aires, Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 520 | 3 | |a The binding of 3H-RO 5-4864 to the peripheral-type benzodiazepine receptors (PBZDR) in rat testicular interstitial cells (TIC) was characterized. The binding was saturable, reversible and showed a single high-affinity (K(d) = 5.02 ± 0.86 nM) class of binding sites. The maximal binding capacity (B(max)) in crude mitochondrial fractions (77.6 ± 9.1 pmol/mg protein) represents the highest density of PBZDR in tissues thus far studied. In comparison with the crude mitochondrial fraction the subcellular fractionation of of TIC revealed a 2-fold enrichment of 3H-RO 5-4864 binding sites to the purified mitochondria (B(max) = 140 ± 23 pmol/mg protein). The ability of various drugs to displace 3H-RO 5-4864 from TIC binding sites was examined and the inhibition constants (K(i)) for RO 5-4864, PK 11195, diazepam and flunitrazepam were 3.5, 4.4, 159, and 353 nM, respectively, whereas clonazepam and RO 15-1788 were inefficient in displacing 3H-RO 5-4864 (K(i) > 10 μM). This pharmacological profile is characteristic of PBZDR described in other tissues. In conclusion, rat TIC possess a very high concentration of PBZDR primarily associated with mitochondrial membranes. |l eng | |
| 593 | |a Inst. de Biologia/Med. Exp., Obligado 2490, 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a BENZODIAZEPINE |
| 690 | 1 | 0 | |a MITOCHONDRIAL MEMBRANES |
| 690 | 1 | 0 | |a PERIPHERAL RECEPTOR |
| 690 | 1 | 0 | |a TESTICULAR INTERSTITIAL CELLS |
| 690 | 1 | 0 | |a 4' CHLORODIAZEPAM |
| 690 | 1 | 0 | |a DIAZEPAM |
| 690 | 1 | 0 | |a FLUNITRAZEPAM |
| 690 | 1 | 0 | |a N SEC BUTYL 1 (2 CHLOROPHENYL) N METHYL 3 ISOQUINOLINECARBOXAMIDE |
| 690 | 1 | 0 | |a RADIOISOTOPE |
| 690 | 1 | 0 | |a RECEPTOR |
| 690 | 1 | 0 | |a ANIMAL CELL |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a INTERSTITIUM |
| 690 | 1 | 0 | |a MALE |
| 690 | 1 | 0 | |a MITOCHONDRION |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a RAT |
| 690 | 1 | 0 | |a TESTIS |
| 690 | 1 | 0 | |a TOPICAL DRUG ADMINISTRATION |
| 653 | 0 | 0 | |a pk 11195; ro 5 4864 |
| 700 | 1 | |a Ritta, M.N. | |
| 700 | 1 | |a Calandra, R.S. | |
| 700 | 1 | |a Medina, J.H. | |
| 773 | 0 | |d 1990 |g v. 52 |h pp. 350-353 |k n. 4 |p NEUROENDOCRINOLOGY |x 00283835 |w (AR-BaUEN)CENRE-6253 |t Neuroendocrinology | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025173643&doi=10.1159%2f000125606&partnerID=40&md5=fdb4861562bc0b431d39aee0b91ea87d |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1159/000125606 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00283835_v52_n4_p350_Calvo |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283835_v52_n4_p350_Calvo |y Registro en la Biblioteca Digital |
| 961 | |a paper_00283835_v52_n4_p350_Calvo |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 80358 | ||