Purification and characterization of a β-glucosidase from the phytopathogenic fungus Fusarium oxysporum f. sp. melonis
Fusarium oxysporum f. sp. melonis produces cellulase and β-glucosidase activities in a medium with glucose and avicel as carbon source. A β-glucosidase from this crude material was purified by gel filtration and ion exchange chromatography successively. This enzyme is a unique band of protein in SDS...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
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Blackwell Publishing Ltd
1996
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 07258caa a22007457a 4500 | ||
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| 001 | PAPER-18926 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518205017.0 | ||
| 008 | 190411s1996 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0029671364 | |
| 024 | 7 | |2 cas |a beta glucosidase, 51683-43-3, 9001-22-3 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a LAMIE | ||
| 100 | 1 | |a Alconada, T.M. | |
| 245 | 1 | 0 | |a Purification and characterization of a β-glucosidase from the phytopathogenic fungus Fusarium oxysporum f. sp. melonis |
| 260 | |b Blackwell Publishing Ltd |c 1996 | ||
| 270 | 1 | 0 | |m Martinez, M.J.; Ctr.Investigaciones Biologicas (CIB), CSIC, Dept. de Microbiologia Molecular, Velazquez 144, E-28006 Madrid, Spain |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Alconada, T.M., Martínez, M.J., Purification and characterization of an extracellular endo-1,4-β-xylanase from Fusarium oxysporum f. sp. melonis (1994) FEMS Microbiological Letters, 118, pp. 305-310 | ||
| 504 | |a Guillén, F., Reyes, F., Rodríguez, J., Vázquez, C., Induction of an extracellular ccllulase system during autolysis of Alternaria alternata (1987) Transactions of the British Mycological Society, 89, pp. 35-39 | ||
| 504 | |a Hrmova, M., Petrakova, E., Biely, P., Induction of cellulose and xylan degrading enzyme systems in Aspergillus terreus by homo and heterodisaccharides composed of glucose and xylose (1991) Journal of General Microbiology, 137, pp. 541-547 | ||
| 504 | |a Martínez, M.J., Reyes, F., Lahoz, R., Cell wall plant polysaccharide-degrading enzymes in autolysis of Botrytis cinerea (1982) Transaction of the British Mycological Society, 78, pp. 395-403 | ||
| 504 | |a Martínez, M.J., Vázquez, C., Guillén, F., Reyes, F., β-Glucosidase from the celluloytic system of Alternaria alternata autolyzed cultures (1988) FEMS Microbiological Letters, 55, pp. 263-268 | ||
| 504 | |a Martínez, M.J., Alconada, T.M., Guillén, F., Vázquez, V., Reyes, F., Pectic activities from Fusarium oxysporum f. sp. melanis: Purification and characterization of an exopolygalacturonase (1991) FEMS Microbiological Letters, 81, pp. 145-150 | ||
| 504 | |a Megnegneau, B., Branchard, M., Effects of fungal culture filtrates on tissue from susceptible and resistant genotypes of muskmelon to Fusarium oxysporum f. sp. melonis (1991) Plant Science, 79, pp. 105-110 | ||
| 504 | |a Messner, R., Hagspiel, K., Kubicek, C.P., Isolation of a β-glucosidase binding and activating polysaccharide from cell walls of Trichoderma reesei (1990) Archives of Microbiology, 154, pp. 150-155 | ||
| 504 | |a Morrisey, J.H., Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivity (1981) Analytical Biochemistry, 117, pp. 307-310 | ||
| 504 | |a Oguntimein, G.B., Moo-Young, M., Production and properties of β-glucosidase by Neurospora sitophila (1991) World Journal of Microbiology and Biotechnology, 7, pp. 4-11 | ||
| 504 | |a Parr, S.R., Some kinetic properties of the β-D-glucosidase (cellobiase) in a commercial cellulase product from Penicillium funiculosum and its relevance in the hydrolysis of cellulose (1983) Enzyme of Microbiology and Technology, 5, pp. 457-462 | ||
| 504 | |a Riou, C., Freyssinet, G., Fevre, M., Purification and characterization of extracellular pectinolytic enzymes produced by Sclerotinia sclerotiorum (1992) Applied of Environmental Microbiology, 58, pp. 578-583 | ||
| 504 | |a Sengupta, S., Ghosh, A.K., Sengupta, S., Purification and characterization of a β-glucosidase (cellobiase) from a mushroom Termitomyces clypeatus (1991) Biochimica et Biophysica Acta, 1076, pp. 215-220 | ||
| 504 | |a Sharma, S., Sandhu, D.K., Bagga, P.S., Physical characterization of isoenzymcs of endo-β-1,4-glucanase and β-glucosidase from Aspergillus species (1990) FEMS Microbiological Letters, 79, pp. 99-104 | ||
| 504 | |a Shewale, J.G., Sadana, J.C., Cellulase and β-glucosidase production by a basidiomycete species (1978) Canadian Journal of Microbiology, 24, pp. 1204-1216 | ||
| 504 | |a Somogyi, M., A new reagent for the determination of sugars (1945) Journal of Biological Chemistry, 160, pp. 61-73 | ||
| 504 | |a Soni, G.L., Bhatia, I.S., Studies on the extracellular cellulase (Cx) from Fusarium oxysporum (1979) Indian Journal of Biochemistry and Biophysics, 16, pp. 223-226 | ||
| 504 | |a Srivastava, S.K., Gopalkrishnan, K.S., Ramachandran, K.B., Kinetic characterization of a crude β-D-glucosidase and Aspergillus wentii (1984) Enzyme of Microbiology and Technology, 6, pp. 508-512 | ||
| 504 | |a Wod, T.M., García-Campayo, V., Enzymology of cellulose degradation (1990) Biodegradation, 1, pp. 147-161 | ||
| 504 | |a Yoshida, N., Fukushima, T., Saito, H., Shimosaka, M., Okazaki, M., Cellulose and xylan degrading enzymes of plant pathogenic fungus Fusarium oxysporum SUB850 (1989) Agricultural and Biological Chemistry, 53, pp. 1829-1836 | ||
| 520 | 3 | |a Fusarium oxysporum f. sp. melonis produces cellulase and β-glucosidase activities in a medium with glucose and avicel as carbon source. A β-glucosidase from this crude material was purified by gel filtration and ion exchange chromatography successively. This enzyme is a unique band of protein in SDS-PAGE and isoelectric focussing. It had a molecular weight of 66,000 and a pI of 5. Using p-nitrophenyl-β-D-glucopyranoside as substrate β-glucosidase shows a K(m), of 210 μmol l -1 , an optimum pH of 5.5 and an optimum reaction temperature of 60°C, being stable in a pH range of 5-7 for 48 h at room temperature. |l eng | |
| 593 | |a Depto. de Cs. Biológicas, Facultad de Cs. Exactas y Naturales, Ciudad Universitaria, Buenos Aires, Argentina | ||
| 593 | |a Ctro. de Invest. Biológicas, CSIC, Depto. de Microbiol. Molecular, Madrid, Spain | ||
| 593 | |a Ctro. de Invest. Biológicas, Consejo Sup. de Invest. Cientificas, Depto. de Microbiologia Molecular, Velázquez 144, E-28006 Madrid, Spain | ||
| 690 | 1 | 0 | |a BETA GLUCOSIDASE |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a ENZYME ACTIVITY |
| 690 | 1 | 0 | |a ENZYME ANALYSIS |
| 690 | 1 | 0 | |a ENZYME PURIFICATION |
| 690 | 1 | 0 | |a FUSARIUM OXYSPORUM |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a TEMPERATURE |
| 690 | 1 | 0 | |a FUNGI |
| 690 | 1 | 0 | |a FUSARIUM OXYSPORUM F. SP. MELONIS |
| 690 | 1 | 0 | |a FUSARIUM SP. |
| 690 | 1 | 0 | |a MELONIS |
| 650 | 1 | 7 | |2 spines |a PH |
| 700 | 1 | |a Martínez, M.J. | |
| 773 | 0 | |d Blackwell Publishing Ltd, 1996 |g v. 22 |h pp. 106-110 |k n. 2 |p LETT. APPL. MICROBIOL. |x 02668254 |w (AR-BaUEN)CENRE-5940 |t Letters in Applied Microbiology | |
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| 856 | 4 | 0 | |u https://doi.org/10.1111/j.1472-765X.1996.tb01120.x |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_02668254_v22_n2_p106_Alconada |y Handle |
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| 999 | |c 79879 | ||